LOCUS       sequence-333142-       10713 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Express 3x flag IREB2, suppressible by doxycycline addition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Terzi EM, Sviderskiy VO, Alvarez SW, Whiten GC, Possemato R
  TITLE     Iron-sulfur cluster deficiency can be sensed by IRP2 and regulates 
            iron homeostasis and sensitivity to ferroptosis independent of IRP1 
            and FBXL5.
  JOURNAL   Sci Adv. 2021 May 26;7(22). pii: 7/22/eabg4302. doi: 
            10.1126/sciadv.abg4302. Print 2021 May.
  PUBMED    34039609
REFERENCE   2  (bases 1 to 10713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-05-26"; volume: "7(22). pii: 7/22/eabg4302. doi"; pages:
            " 10.1126/sciadv.abg4302. Print 2021 May"
COMMENT     Sequence Label: pCW57.1 blast OFF 3xflag IREB2
FEATURES             Location/Qualifiers
     source          1..10713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    34..52
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    69..87
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    105..123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    141..159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    176..194
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    212..230
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     277..301
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             332..397
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        3628..4138
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4057..4075
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4148..4540
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4550..4603
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4604..5221,5222..5227,5228..5263,5264..5266,5267..5302,
                     5303..5305,5306..5341,5342..5350)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5390..5410)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5808..5819)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6024..6257
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6329..6450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6366..6385)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6420..6439
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6490..6625
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6625..6696
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6697..6765,6766..7557)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6915..6934)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7728..8316
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8217..8236
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8470..8487
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8604..8625
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8640..8645,8646..8663,8664..8670)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8678..8694
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8683..8705
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8702..8718
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8702..8718
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8737..8757
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8739..8757
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8785..9011
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9012..9192
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9239..9364
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9857..10090
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10275..10319
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10532..10649
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10703..10713,1..304)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    join(10711..10713,1..16)
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgatgtc
       61 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
      121 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttatccct
      181 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      241 aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
      301 cgcctggaga attggctagc ctcgagacat ggactacaaa gaccatgacg gtgattataa
      361 agatcatgac atcgattaca aggatgacga tgacaagatg gacgccccaa aagcaggata
      421 cgcctttgag taccttattg aaacattaaa tgacagttca cataagaagt tcttcgatgt
      481 atctaaactt ggcaccaagt atgatgttct gccttactca atacgggtct tgttggaagc
      541 tgctgtacga aattgtgatg gctttttaat gaagaaggaa gatgttatga acattttaga
      601 ctggaaaacc aaacaaagca atgttgaagt gccctttttc cctgcccgtg ttcttcttca
      661 agattttact ggaataccag caatggtgga ttttgctgct atgagggagg cagtgaaaac
      721 tcttggaggt gatcctgaga aagtccatcc tgcttgtccg acagatctta cagttgacca
      781 ttctttacaa attgacttca gtaaatgtgc aatacagaat gcaccgaacc caggcggtgg
      841 tgacctgcag aaagcaggaa agctctctcc acttaaagtg cagcctaaga agcttccctg
      901 cagaggccag actacctgcc gaggatcttg tgattctgga gaactaggcc gaaactcagg
      961 aacattttct tcgcagattg agaatacacc catcctgtgt ccttttcatt tgcaaccagt
     1021 gcctgaacct gaaacagtgt taaaaaatca agaagtagaa ttcggcagaa atcgagagag
     1081 gcttcagttt tttaagtgga gttcaagagt ttttaagaat gtggcagtga tccctcctgg
     1141 gaccgggatg gcccatcaaa taaacttaga atatttgtca agagtggttt ttgaagaaaa
     1201 agacctcctc ttcccagaca gtgtagtcgg cacagattca cacataacga tggtgaatgg
     1261 tttagggatt ctggggtggg gggttggagg cattgaaaca gaagcagtta tgcttggtct
     1321 gccagtttct cttactttac cagaggtggt tggatgtgag ttaactgggt catcaaaccc
     1381 ttttgttaca tccatagatg ttgttcttgg tattacaaag cacctcaggc aagtaggagt
     1441 ggctggaaag tttgttgagt tttttggaag tggagtttca caattatcta tagttgatcg
     1501 aactacaata gcaaacatgt gtccggaata tggtgctatc ctcagctttt tccctgttga
     1561 caatgtgaca ttaaaacatt tagaacatac aggttttagc aaagccaaac tcgaatcaat
     1621 ggaaacatac cttaaagctg tgaaattgtt tcgaaatgac cagaattctt caggagaacc
     1681 tgaatactcc caggtgatcc agattaatct gaattcaata gttccatctg ttagtggtcc
     1741 aaaaagacct caggatagag ttgctgtgac agatatgaaa agcgatttcc aggcttgctt
     1801 aaatgaaaag gttggattta aaggcttcca aattgcagct gaaaaacaaa aggatattgt
     1861 ctccattcat tatgaaggaa gtgaatataa gctgtctcat ggatcagtgg tcattgctgc
     1921 agttatcagt tgtaccaata attgcaatcc atctgtcatg cttgctgcag gtcttttggc
     1981 taaaaaggct gttgaagctg gtctgcgtgt taaaccttat ataagaacaa gtttatctcc
     2041 aggcagtggg atggttacac attacctcag ttcaagtgga gtattaccat atctaagtaa
     2101 gcttggattt gaaatcgttg gctatggatg ttcaacttgt gtgggaaata cagcaccctt
     2161 atcagacgca gttttaaatg cagtaaaaca gggtgatttg gttacctgtg gaattttatc
     2221 tggaaacaaa aattttgaag gtcgtctttg tgattgtgtt cgtgccaatt atcttgcctc
     2281 tccaccctta gtggtagctt atgccatagc aggcacagtg aatatagatt tccagacaga
     2341 acctttaggt actgacccca ccggcaagaa catttacctg catgatattt ggcctagtcg
     2401 agaagaagtt catcgagtag aggaagaaca tgttatacta tccatgttta aagcattaaa
     2461 agataaaata gaaatgggga ataaacggtg gaattcctta gaagcaccgg attcagtttt
     2521 gtttccatgg gacttaaagt ctacttatat cagatgccct tcattttttg ataaacttac
     2581 caaagagcca attgcactcc aggctattga aaatgcccat gtcttattat atttgggaga
     2641 ctctgtcaca acagatcata tatcacctgc aggaagtatc gctaggaata gtgctgccgc
     2701 taagtatttg acaaacagag gccttacccc tcgtgaattc aactcttacg gagctcgaag
     2761 aggtaatgat gctgtaatga caagaggcac ttttgcaaat atcaagcttt ttaataagtt
     2821 tattggaaaa ccagctccta aaacaattca ttttccatca ggacagacgc tagatgtatt
     2881 tgaggctgca gagctgtacc agaaagaagg tatcccactg attattttag caggaaagaa
     2941 atatggttca ggaaactcca gagactgggc tgccaaagga ccgtatttac tgggtgtgaa
     3001 agctgttttg gctgaaagtt atgaaaaaat acacaaagat catttgattg gaattggcat
     3061 agctccactt cagttccttc caggagaaaa tgcagattcc ttgggcctct ccggtagaga
     3121 aacattttct ttaacatttc ctgaagaact gtctcctgga attacattga atatacagac
     3181 aagcactgga aaagtattca gcgtgattgc ttcgtttgaa gatgatgtgg aaataacatt
     3241 atacaaacat ggaggattat taaactttgt ggcacgaaaa ttctcatagt atcgcggccg
     3301 cgtcgaccgt tcagctgctt atgctgctcg ttgggtggca aaatcccttg ttaaaggagg
     3361 tctgtgccgg agagttcttg ttcaggtctc ttatgctatt ggagtttctc atccattatc
     3421 tatctccatt ttccattatg gtacctctca gaagagtgag agagagctat tagagattgt
     3481 gaagaagaat ttcgatctcc gccctggggt cattgtcagg gatctggatc tgaagaagcc
     3541 aatttatcag aggactgcag cctatggcca ctttggtagg gacagcttcc catgggaagt
     3601 gcccaaaaag cttaaatatt gaggtccggg gttggggttg cgccttttcc aaggcagccc
     3661 tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga
     3721 ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac
     3781 ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg
     3841 gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga
     3901 cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg
     3961 ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg
     4021 tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc
     4081 ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccagca
     4141 attcaccatg gttcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     4201 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     4261 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     4321 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     4381 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     4441 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     4501 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc cccggtgccg aaggtagagg
     4561 ttctctcctc acttgtggtg atgttgaaga aaaccctggt ccaatgtcta gactggacaa
     4621 gagcaaagtc ataaactctg ctctggaatt actcaatgaa gtcggtatcg aaggcctgac
     4681 gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa
     4741 gaacaagcgg gccctgctcg atgccctggc aatcgagatg ctggacaggc atcataccca
     4801 cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt
     4861 ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac
     4921 agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc
     4981 cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt
     5041 ggaggatcag gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat
     5101 gcccccactt ctgagacaag caattgagct gttcgaccat cagggagccg aacctgcctt
     5161 ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg
     5221 cgggccggcc gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga
     5281 cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct
     5341 ccccgggtaa ctaagtaagg atcgatccaa gatatcgtat tcttaactat gttgctcctt
     5401 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5461 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5521 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5581 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5641 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5701 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     5761 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5821 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5881 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgtt tcgcctcggc
     5941 gtccggacta gaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6001 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     6061 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6121 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6181 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6241 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     6301 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     6361 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6421 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     6481 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6541 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     6601 tagtgaggag gcttttttgg aggcctttca aatatgtatc cgctcatgag acaataaccc
     6661 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6721 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6781 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6841 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6901 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6961 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7021 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7081 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7141 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7201 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7261 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7321 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7381 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7441 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7501 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7561 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7621 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7681 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7741 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7801 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7861 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7921 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7981 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8041 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8101 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8161 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8221 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8281 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8341 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8401 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8461 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8521 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     8581 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     8641 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     8701 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     8761 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     8821 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     8881 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     8941 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     9001 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9061 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9121 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9181 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     9241 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     9301 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     9361 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     9421 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     9481 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     9541 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     9601 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     9661 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     9721 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     9781 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     9841 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     9901 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     9961 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
    10021 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
    10081 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
    10141 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
    10201 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
    10261 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
    10321 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
    10381 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
    10441 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga caattctcga
    10501 cctcgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
    10561 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    10621 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
    10681 tttggccgcg aatcgatatg tcgagtttac tcc
//
