LOCUS       sequence-333278-        4193 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Constitutively expressed, chimeric transcription activator 
            comprising the AraC DNA binding domain and the UreR urea ligand 
            binding domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, 
            Swint-Kruse L
  TITLE     Allosteric regulation within the highly interconnected structural 
            scaffold of AraC/XylS homologs tolerates a wide range of amino acid 
            changes.
  JOURNAL   Proteins. 2021 Aug 8. doi: 10.1002/prot.26206.
  PUBMED    34369028
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2021 Aug 8. doi: 10.1002/prot.26206."
COMMENT     Sequence Label: UUUUA-pHG165c
FEATURES             Location/Qualifiers
     source          1..4193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(26..614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(106..125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(785..1576,1577..1645))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1408..1427
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1646..1750)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1818..1836
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     1989..2011
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2003..2020
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2004..2020
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3600..3791
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3785..3807)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3893..4033
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3948..3967
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4048..4065)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
       61 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      121 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      181 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      241 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      301 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      361 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      421 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
      481 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      541 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      601 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      661 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      721 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      781 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      841 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
      901 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
      961 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1021 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1081 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1141 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1201 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1261 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1321 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1381 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1441 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1501 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1561 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1621 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1681 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1741 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     1801 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaatccgtta
     1861 acggcattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     1921 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     1981 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttg gctgcaggtc
     2041 gacggatccc cgggaattcc accatcgaat ggtgacacca tcgaatggtg caaaaccttt
     2101 cgcggtatgg catgatagcg cccggggccc aagttcactt aaaaaggaga tcaacaatga
     2161 agcaattttc gtactgaaac atcttaatca tgctggggag ggtttctaat ggaatataaa
     2221 ttctttcttt cagaaagcca gatggtgtta aaagcgtttt atattgaaag cgcgatgata
     2281 gccatgctaa ctggcgcgaa aggaaatatc gtgatcaatg gccaatccat tgaaattgaa
     2341 agcgatgtca ccttaattat tcctaaatac agccaagttt cctgcaatat tgtgtgtaat
     2401 accgtgaagc agccgcttga gttgcacacg ttgtcgatgt cagcagaaga attacaagcg
     2461 gtatttttat tactgaaaac attgatgaag tccggtgcgc ctctgactag ccaccaaccc
     2521 atctaccata tttctccccc agaaactgtg agagataact ttagtttgct aaaacaatgc
     2581 ttaccattaa aaaaacagag tgcatcccaa gaagcattgt taatgaaaca gagcctatat
     2641 tttattttaa tggcgatata tcaagaaggt atcgatattc tgaatctttt tcgtttcaat
     2701 tacgatgaag ataatcgggt acgcgaggct tgtcagtaca tcagcgatca cctggcagac
     2761 agcaattttg atatcgccag cgtcgcacag catgtttgct tgtcgccgtc gcgtctgtca
     2821 catcttttcc gccagcagtt agggattagc gtcttaagct ggcgcgagga ccaacgtatc
     2881 agccaggcga agctgctttt gagcaccacc cggatgccta tcgccaccgt cggtcgcaat
     2941 gttggttttg acgatcaact ctatttctcg cgggtattta aaaaatgcac cggggccagc
     3001 ccgagcgagt tccgtgccgg ttgtgaagaa aaagtgaatg atgtagccgt caagttgtca
     3061 taatgagaat tcgtaataat atagctctag attaattgcg cccgttaacg gcgggcagcg
     3121 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
     3181 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
     3241 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
     3301 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
     3361 tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
     3421 attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
     3481 cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
     3541 ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag
     3601 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     3661 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     3721 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     3781 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     3841 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     3901 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     3961 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     4021 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4081 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4141 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga acc
//
