LOCUS       sequence-333364-        4596 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 SARS-CoV-1 NSP13_nostop
FEATURES             Location/Qualifiers
     source          1..4596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1939..2038)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2055..2074)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2056..2074)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2079..2095)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2079..2095)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2526..3317
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3410..3998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3899..3918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4152..4169
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4328..4355)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4447..4533)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4582..4596,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4596,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagttgg catggctgta ggtgcttgtg tattgtgcaa ttcacagact tcacttcgtt
      181 gcggtgcctg tattaggagg ccattcctat gttgcaagtg ctgctatgac catgtcattt
      241 caacatcaca caaattagtg ttgtctgtta atccctatgt ttgcaatgcc ccaggttgtg
      301 atgtcactga tgtgacacaa ctgtatctag gaggtatgag ctattattgc aagtcacata
      361 agcctcccat tagttttcca ttatgtgcta atggtcaggt ttttggttta tacaaaaaca
      421 catgtgtagg cagtgacaat gtcactgact tcaatgcgat agcaacatgt gattggacta
      481 atgctggcga ttacatactt gccaacactt gtactgagag actcaagctt ttcgcagcag
      541 aaacgctcaa agccactgag gaaacattta agctgtcata tggtattgcc actgtacgcg
      601 aagtactctc tgacagagaa ttgcatcttt catgggaggt tggaaaacct agaccaccat
      661 tgaacagaaa ctatgtcttt actggttacc gtgtaactaa aaatagtaaa gtacagattg
      721 gagagtacac ctttgaaaaa ggtgactatg gtgatgctgt tgtgtacaga ggtactacga
      781 catacaagtt gaatgttggt gattactttg tgttgacatc tcacactgta atgccactta
      841 gtgcacctac tctagtgcca caagagcact atgtgagaat tactggcttg tacccaacac
      901 tcaacatctc agatgagttt tctagcaatg ttgcaaatta tcaaaaggtc ggcatgcaaa
      961 agtactctac actccaagga ccacctggta ctggtaagag tcattttgcc atcggacttg
     1021 ctctctatta cccatctgct cgcatagtgt atacggcatg ctctcatgca gctgttgatg
     1081 ccctatgtga aaaggcatta aaatatttgc ccatagataa atgtagtaga atcatacctg
     1141 cgcgtgcgcg cgtagagtgt tttgataaat tcaaagtgaa ttcaacacta gaacagtatg
     1201 ttttctgcac tgtaaatgca ttgccagaaa caactgctga cattgtagtc tttgatgaaa
     1261 tctctatggc tactaattat gacttgagtg ttgtcaatgc tagacttcgt gcaaaacact
     1321 acgtctatat tggcgatcct gctcaattac cagccccccg cacattgctg actaaaggca
     1381 cactagaacc agaatatttt aattcagtgt gcagacttat gaaaacaata ggtccagaca
     1441 tgttccttgg aacttgtcgc cgttgtcctg ctgaaattgt tgacactgtg agtgctttag
     1501 tttatgacaa taagctaaaa gcacacaagg ataagtcagc tcaatgcttc aaaatgttct
     1561 acaaaggtgt tattacacat gatgtttcat ctgcaatcaa cagacctcaa ataggcgttg
     1621 taagagaatt tcttacacgc aatcctgctt ggagaaaagc tgtttttatc tcaccttata
     1681 attcacagaa cgctgtagct tcaaaaatct taggattgcc tacgcagact gttgattcat
     1741 cacagggttc tgaatatgac tatgtcatat tcacacaaac tactgaaaca gcacactctt
     1801 gtaatgtcaa ccgcttcaat gtggctatca caagggcaaa aattggcatt ttgtgcataa
     1861 tgtctgatag agatctttat gacaaactgc aatttacaag tctagaaata ccacgtcgca
     1921 atgtggctac attacaatac ccaactttct tgtacaaagt tggcattata agaaagcatt
     1981 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     2041 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     2101 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2161 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     2221 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     2281 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     2341 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     2401 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     2461 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     2521 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     2581 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     2641 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     2701 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2761 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2821 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2881 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2941 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     3001 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     3061 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     3121 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     3181 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     3241 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     3301 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     3361 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3421 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3481 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3541 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3601 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3661 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3721 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3781 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3841 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3901 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3961 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4021 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4081 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4141 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     4201 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     4261 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     4321 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     4381 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     4441 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     4501 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
     4561 taacgctagc atggatgttt tcccagtcac gacgtt
//
