LOCUS       sequence-333877-       13397 bp    DNA     circular SYN 15-APR-2025
DEFINITION  puromycin resistant lentiviral vector for expression of PAK5 K478R 
            K469R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   LaPak KM, Vroom DC, Garg AA, Guan X, Hays JL, Song JW, Burd CE
  TITLE     Melanoma-associated mutants within the serine-rich domain of PAK5 
            direct kinase activity to mitogenic pathways.
  JOURNAL   Oncotarget. 2018 May 22;9(39):25386-25401. doi: 
            10.18632/oncotarget.25356. eCollection 2018 May 22.
  PUBMED    29875996
REFERENCE   2  (bases 1 to 13397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.25356"; journalName: "Oncotarget"; date: 
            "2018-05-22- 22"; volume: "9"; issue: "39"; pages: "25386-25401"
COMMENT     Sequence Label: pLenti-3xFLAG-Puro PAK5 K478R K469R
FEATURES             Location/Qualifiers
     source          1..13397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(389..1180,1181..1249))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1012..1031
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1250..1354)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1402..1536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             1704..2337
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2384..2509
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3006..3239
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3424..3468
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3766..3883
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3948..4322)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      4319..4328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4332..4350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4342..4389)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     5407..5426
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5586..5651
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7877..8450
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8045..8062)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8272..8291
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8453..9055
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8952..8972
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9071..9659
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9124..9144)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9542..9553)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9866..10099
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10200..10217)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10206..10430
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10476..10904
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10563..10582)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10773..10794
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10913..10933)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10918..11247
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11098..11233
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11160..11179
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    12455..12576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12625..12641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12625..12641)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12638..12660)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12649..12665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12673..12679,12680..12697,12698..12703))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12718..12739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12856..12873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(13027..13397,1..218))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13107..13126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
       61 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      121 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      181 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      241 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      301 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
      361 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
      421 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
      481 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
      541 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
      601 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
      661 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
      721 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
      781 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
      841 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
      901 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
      961 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     1021 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1081 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     1141 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1201 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1261 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     1321 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     1381 acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc
     1441 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1501 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     1561 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     1621 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     1681 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     1741 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     1801 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     1861 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     1921 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     1981 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     2041 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     2101 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     2161 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     2221 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     2281 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     2341 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     2401 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     2461 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     2521 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     2581 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     2641 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     2701 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     2761 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     2821 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     2881 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     2941 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     3001 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     3061 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     3121 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     3181 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     3241 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     3301 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     3361 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     3421 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     3481 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     3541 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     3601 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     3661 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     3721 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     3781 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     3841 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     3901 cagcagagat ccagtttggt tagtaccggg cccgctctag tccggaatca gtcctgctcc
     3961 tcggccacga agtgcacgca gttgccggcc gggtcgcgca gggcgaactc ccgcccccac
     4021 ggctgctcgc cgatctcggt catggccggc ccggaggcgt cccggaagtt cgtggacacg
     4081 acctccgacc actcggcgta cagctcgtcc aggccgcgca cccacaccca ggccagggtg
     4141 ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc
     4201 acaccggcga agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc ggtccagaac
     4261 tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt caacttggcc
     4321 atggtggccc tcctatagtg agtcgtatta tactatgccg atatactatg ccgatgatta
     4381 attgtcaaca cgtgctgcag gtccgaggtt ctaggctgcc acgtcagacg aagggcgcag
     4441 cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc
     4501 ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag
     4561 ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga
     4621 ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg
     4681 tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg
     4741 ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc
     4801 gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg
     4861 cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc
     4921 gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg
     4981 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
     5041 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
     5101 ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt
     5161 gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg
     5221 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
     5281 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
     5341 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
     5401 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
     5461 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
     5521 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac gctagcggat
     5581 ccatggacta caaagaccat gacggtgatt ataaagatca tgatatcgat tacaaggatg
     5641 acgatgacaa gctagcgatg tttgggaaga aaaagaaaaa gattgaaata tctggcccgt
     5701 ccaactttga acacagggtt catactgggt ttgatccaca agagcagaag tttaccggcc
     5761 ttccccagca gtggcacagc ctgttagcag atacggccaa caggccaaag cctatggtgg
     5821 acccttcatg catcacaccc atccagctgg ctcctatgaa gacaatcgtt agaggaaaca
     5881 aaccctgcaa ggaaacctcc atcaacggcc tgctagagga ttttgacaac atctcggtga
     5941 ctcgctccaa ctccctaagg aaagaaagcc cacccacccc agatcaggga gcctccagcc
     6001 acggtccagg ccacgcggaa gaaaatggct tcatcacctt ctcccagtat tccagcgaat
     6061 ccgatactac tgctgactac acgaccgaaa agtacaggga gaagagtctc tatggagatg
     6121 atctggatcc gtattataga ggcagccacg cagccaagca aaatgggcac gtaatgaaaa
     6181 tgaagcacgg ggaggcctac tattctgagg tgaagccttt gaaatccgat tttgccagat
     6241 tttctgccga ttatcactca catttggact cactgagcaa accaagtgaa tacagtgacc
     6301 tcaagtggga gtatcagaga gcctcgagta gctcccctct ggattattca ttccaattca
     6361 caccttctag aactgcaggg accagcgggt gctccaagga gagcctggcg tacagtgaaa
     6421 gtgaatgggg acccagcctg gatgactatg acaggaggcc aaagtcttcg tacctgaatc
     6481 agacaagccc tcagcccacc atgcggcaga ggtccaggtc aggctcggga ctccaggaac
     6541 cgatgatgcc atttggagca agtgcattta aaacccatcc ccaaggacac tcctacaact
     6601 cctacaccta ccctcgcttg tccgagccca caatgtgcat tccaaaggtg gattacgatc
     6661 gagcacagat ggtcctcagc cctccactgt cagggtctga cacctacccc aggggccctg
     6721 ccaaactacc tcaaagtcaa agcaaatcgg gctattcctc aagcagtcac cagtacccgt
     6781 ctgggtacca caaagccacc ttgtaccatc acccctccct gcagagcagt tcgcagtaca
     6841 tctccacggc ttcctacctg agctccctca gcctctcatc cagcacctac ccgccgccca
     6901 gctggggctc ctcctccgac cagcagccct ccagggtgtc ccatgaacag tttcgggcgg
     6961 ccctgcagct ggtggtcagc ccaggagacc ccagggaata cttggccaac tttatcaaaa
     7021 tcggggaagg ctcaaccggc atcgtatgca tcgccaccga gaaacacaca gggaaacaag
     7081 ttgcagtgag gagaatggac ctccggaagc aacagagacg agaactgctt ttcaatgagg
     7141 tcgtgatcat gcgggattac caccatgaca atgtggttga catgtacagc agctaccttg
     7201 tcggcgatga gctctgggtg gtcatggagt ttctagaagg tggtgccttg acagacattg
     7261 tgactcacac cagaatgaat gaagaacaga tagctactgt ctgcctgtca gttctgagag
     7321 ctctctccta ccttcataac caaggagtga ttcacaggga cataaaaagt gactccatcc
     7381 tcctgacaag cgatggccgg ataaagttgt ctgattttgg tttctgtgct caagtttcca
     7441 aagaggtgcc gaagaggaaa tcattggttg gcactcccta ctggatggcc cctgaggtga
     7501 tttctaggct accttatggg acagaggtgg acatctggtc cctcgggatc atggtgatag
     7561 aaatgattga tggcgagccc ccctacttca atgagcctcc cctccaggcg atgcggagga
     7621 tccgggacag tttacctcca agagtgaagg acctacacaa ggtttcttca gtgctccggg
     7681 gattcctaga cttgatgttg gtgagggagc cctctcagag agcaacagcc caggaactcc
     7741 tcggacatcc attcttaaaa ctagcaggtc caccgtcttg cattgtcccc ctcatgagac
     7801 aatacaggca tcactgaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga
     7861 gttaacgcaa attccgcccc tctccctccc ccccccccta acgttactgg ccgaagccgc
     7921 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     7981 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     8041 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     8101 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     8161 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     8221 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     8281 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     8341 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     8401 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata ccatggccac
     8461 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
     8521 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
     8581 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     8641 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     8701 cgaagcgggg gcggtgttcg ccgagatcgg ctcgcgcatg gccgagttga gcggttcccg
     8761 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     8821 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     8881 cgtcgtgctc cccggagtgg aggcggccga gcgcgctggg gtgcccgcct tcctggagac
     8941 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     9001 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagttcg
     9061 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     9121 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     9181 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     9241 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     9301 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     9361 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     9421 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     9481 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     9541 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     9601 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     9661 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca
     9721 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     9781 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     9841 cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat
     9901 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     9961 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
    10021 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
    10081 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
    10141 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gtttaaaccc gctgatcagc
    10201 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
    10261 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
    10321 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
    10381 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
    10441 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
    10501 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
    10561 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
    10621 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
    10681 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
    10741 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    10801 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
    10861 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
    10921 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    10981 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
    11041 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
    11101 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
    11161 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
    11221 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
    11281 tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
    11341 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
    11401 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
    11461 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
    11521 gaagtgcttg acattgggga attaattcag cgagagcctg acctattgca tctcccgccg
    11581 tgcacagggt gtcacgtcgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
    11641 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
    11701 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
    11761 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
    11821 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
    11881 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
    11941 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
    12001 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
    12061 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
    12121 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
    12181 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
    12241 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
    12301 cactcgtccg agggcaaagg aatagcacgt gctacgagat ttcgattcca ccgccgcctt
    12361 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    12421 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
    12481 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    12541 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
    12601 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    12661 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    12721 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    12781 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    12841 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    12901 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    12961 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    13021 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    13081 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    13141 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    13201 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    13261 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    13321 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    13381 cactggtaac aggatta
//
