LOCUS       sequence-334156-        8122 bp    DNA     circular SYN 14-APR-2025
DEFINITION  pDL17 is based on conditionally replicating backbone derived from 
            pQE-30 and expresses gam, bet and exo genes under control of PrhaB 
            promoter for efficient dsDNA integration into E. coli chromosome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Bubnov DM, Yuzbashev TV, Vybornaya TV, Netrusov AI, Sineoky SP
  TITLE     Development of new versatile plasmid-based systems for 
            lambdaRed-mediated Escherichia coli genome engineering.
  JOURNAL   J Microbiol Methods. 2018 Jun 7. pii: S0167-7012(18)30368-3. doi: 
            10.1016/j.mimet.2018.06.001.
  PUBMED    29885886
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods. 2018 Jun 7. pii: S0167-7012(18)30368-3. doi: 
            10.1016/j.mimet.2018.06.001."
COMMENT     Sequence Label: pDL17
FEATURES             Location/Qualifiers
     source          1..8122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..119
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring 
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     CDS             153..569
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             575..1360
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             1357..2037
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     terminator      2038..2282
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     terminator      2443..2529
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2621..2648
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        complement(join(2683..2689,2690..2707,2708..2713))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2728..2749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(2762..3844)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3806..3825
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3845..3922)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(3969..3983,3984..3989,3990..4006,4007..4013)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    3989..4005
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4021..4037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4056..4067
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4086..4103
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4175..4192)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4346..4934)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4426..4445)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5105..5896,5897..5965))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5728..5747
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5966..6071)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      6097..6191
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(6219..7067)
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
     CDS             complement(7141..7977)
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
ORIGIN
        1 caccacaatt cagcaaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc
       61 ccattttcct gtcagtaacg agaaggtcgc gaattcaggc gctttttaga ctggtcgtaa
      121 tgaaattcgg atcccgtcga cgcttataaa aaatggatat taatactgaa actgagatca
      181 agcaaaagca ttcactaacc ccctttcctg ttttcctaat cagcccggca tttcgcgggc
      241 gatattttca cagctatttc aggagttcag ccatgaacgc ttattacatt caggatcgtc
      301 ttgaggctca gagctgggcg cgtcactacc agcagctcgc ccgtgaagag aaagaggcag
      361 aactggcaga cgacatggaa aaaggcctgc cccagcacct gtttgaatcg ctatgcatcg
      421 atcatttgca acgccacggg gccagcaaaa aatccattac ccgtgcgttt gatgacgatg
      481 ttgagtttca ggagcgcatg gcagaacaca tccggtacat ggttgaaacc attgctcacc
      541 accaggttga tattgattca gaggtataaa acgaatgagt actgcactcg caacgctggc
      601 tgggaagctg gctgaacgtg tcggcatgga ttctgtcgac ccacaggaac tgatcaccac
      661 tcttcgccag acggcattta aaggtgatgc cagcgatgcg cagttcatcg cattactgat
      721 cgttgccaac cagtacggcc ttaatccgtg gacgaaagaa atttacgcct ttcctgataa
      781 gcagaatggc atcgttccgg tggtgggcgt tgatggctgg tcccgcatca tcaatgaaaa
      841 ccagcagttt gatggcatgg actttgagca ggacaatgaa tcctgtacat gccggattta
      901 ccgcaaggac cgtaatcatc cgatctgcgt taccgaatgg atggatgaat gccgccgcga
      961 accattcaaa actcgcgaag gcagagaaat cacggggccg tggcagtcgc atcccaaacg
     1021 gatgttacgt cataaagcca tgattcagtg tgcccgtctg gccttcggat ttgctggtat
     1081 ctatgacaag gatgaagccg agcgcattgt cgaaaatact gcatacactg cagaacgtca
     1141 gccggaacgc gacatcactc cggttaacga tgaaaccatg caggagatta acactctgct
     1201 gatcgccctg gataaaacat gggatgacga cttattgccg ctctgttccc agatatttcg
     1261 ccgcgacatt cgtgcatcgt cagaactgac acaggccgaa gcagtaaaag ctcttggatt
     1321 cctgaaacag aaagccgcag agcagaaggt ggcagcatga caccggacat tatcctgcag
     1381 cgtaccggga tcgatgtgag agctgtcgaa cagggggatg atgcgtggca caaattacgg
     1441 ctcggcgtca tcaccgcttc agaagttcac aacgtgatag caaaaccccg ctccggaaag
     1501 aagtggcctg acatgaaaat gtcctacttc cacaccctgc ttgctgaggt ttgcaccggt
     1561 gtggctccgg aagttaacgc taaagcactg gcctggggaa aacagtacga gaacgacgcc
     1621 agaaccctgt ttgaattcac ttccggcgtg aatgttactg aatccccgat catctatcgc
     1681 gacgaaagta tgcgtaccgc ctgctctccc gatggtttat gcagtgacgg caacggcctt
     1741 gaactgaaat gcccgtttac ctcccgggat ttcatgaagt tccggctcgg tggtttcgag
     1801 gccataaagt cagcttacat ggcccaggtg cagtacagca tgtgggtgac gcgaaaaaat
     1861 gcctggtact ttgccaacta tgacccgcgt atgaagcgtg aaggcctgca ttatgtcgtg
     1921 attgagcggg atgaaaagta catggcgagt tttgacgaga tcgtgccgga gttcatcgaa
     1981 aaaatggacg aggcactggc tgaaattggt tttgtatttg gggagcaatg gcgatgacgc
     2041 atcctcacga taatatccgg gtaggcgcaa tcactttcgt ctactccgtt acaaagcgag
     2101 gctgggtatt tcccggcctt tctgttatcc gaaatccact gaaagcacag cggctggctg
     2161 aggagataaa taataaacga ggggctgtat gcacaaagca tcttctgttg agttaagaac
     2221 gagtatcgag atggcacata gccttgctca aattggaatc aggtttgtgc caataccagt
     2281 agaaacagac gaagaattct aggctggaga atctcgatcc ggactccctg acgccacgcc
     2341 aggcgctgga gtggatctat cggctgaaaa gtctggtgta aggatcgtgg cagacaagcc
     2401 cgtcagggcg cgtccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     2461 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     2521 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     2581 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
     2641 ggcctttttg cgtttctaca aactcttcct gccatggcca cacaacatac gagccggaag
     2701 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     2761 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2821 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
     2881 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
     2941 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
     3001 aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca
     3061 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
     3121 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
     3181 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
     3241 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
     3301 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg
     3361 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
     3421 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
     3481 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
     3541 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
     3601 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
     3661 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
     3721 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     3781 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     3841 tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     3901 ttttgcacca ttcgatggtg tcaacgtaaa tgcatgccgc taggcccttt cgtcttcacc
     3961 tcgagaaatc ataaaaaatt tatttgcttt gtgagcggat aacaattata atagattcaa
     4021 ttgtgagcgg ataacaattt cacacagaat tcattaaaga ggagaaatta actatgagag
     4081 gatcgcatca ccatcaccat cacggatcta tgcggtgtga aataccgcac agatgcgtaa
     4141 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4201 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4261 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4321 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4381 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4441 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4501 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4561 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4621 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4681 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4741 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     4801 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4861 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4921 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4981 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5041 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5101 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5161 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5221 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5281 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5341 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5401 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5461 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5521 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5581 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5641 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5701 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5761 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5821 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5881 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5941 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6001 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6061 ggggttccgc gcacatttcc ccgaaaagtg ccacctgact cctgttgata gatccagtaa
     6121 tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
     6181 ttggtgagaa tgacgtccgt cggcatcggc atggcgaatt aatctttctg cgaattgaga
     6241 tgacgccact ggctgggcgt catcccggtt tcccgggtaa acaccaccga aaaatagtta
     6301 ctatcttcaa agccacattc ggtcgaaata tcactgatta acaggcggct atgctggaga
     6361 agatattgcg catgacacac tctgacctgt cgcagatatt gattgatggt cattccagtc
     6421 tgctggcgaa attgctgacg caaaacgcgc tcactgcacg atgcctcatc acaaaattta
     6481 tccagcgcaa agggactttt caggctagcc gccagccggg taatcagctt atccagcaac
     6541 gtttcgctgg atgttggcgg caacgaatca ctggtgtaac gatggcgatt cagcaacatc
     6601 accaactgcc cgaacagcaa ctcagccatt tcgttagcaa acggcacatg ctgactactt
     6661 tcatgctcaa gctgaccgat aacctgccgc gcctgcgcca tccccatgct acctaagcgc
     6721 cagtgtggtt gccctgcgct ggcgttaaat cccggaatcg ccccctgcca gtcaagattc
     6781 agcttcagac gctccgggca ataaataata ttctgcaaaa ccagatcgtt aacggaagcg
     6841 taggagtgtt tatcgtcagc atgaatgtaa aagagatcgc cacgggtaat gcgataaggg
     6901 cgatcgttga gtacatgcag gccattaccg cgccagacaa tcaccagctc acaaaaatca
     6961 tgtgtatgtt cagcaaagac atcttgcgga taacggtcag ccacagcgac tgcctgctgg
     7021 tcgctggcaa aaaaatcatc tttgagaagt tttaactgat gcgccaccgt ggctacctcg
     7081 gccagagaac gaagttgatt attcgcaata tggcgtacaa atacgttgag aagattcgcg
     7141 ttattgcaga aagccatccc gtccctggcg aatatcacgc ggtgaccagt taaactctcg
     7201 gcgaaaaagc gtcgaaaagt ggttactgtc gctgaatcca cagcgatagg cgatgtcagt
     7261 aacgctggcc tcgctgtggc gtagcagatg tcgggctttc atcagtcgca ggcggttcag
     7321 gtatcgctga ggcgtcagtc ccgtttgctg cttaagctgc cgatgtagcg tacgcagtga
     7381 aagagaaaat tgatccgcca cggcatccca attcacctca tcggcaaaat ggtcctccag
     7441 ccaggccaga agcaagttga gacgtgatgc gctgttttcc aggttctcct gcaaactgct
     7501 tttacgcagc aagagcagta attgcataaa caagatctcg cgactggcgg tcgagggtaa
     7561 atcattttcc ccttcctgct gttccatctg tgcaaccagc tgtcgcacct gctgcaatac
     7621 gctgtggtta acgcgccagt gagacggata ctgcccatcc agctcttgtg gcagcaactg
     7681 attcagcccg gcgagaaact gaaatcgatc cggcgagcga tacagcacat tggtcagaca
     7741 cagattatcg gtatgttcat acagatgccg atcatgatcg cgtacgaaac agaccgtgcc
     7801 accggtgatg gtatagggct gcccattaaa cacatgaata cccgtgccat gttcgacaat
     7861 cacaatttca tgaaaatcat gatgatgttc aggaaaatcc gcctgcggga gccggggttc
     7921 tatcgccacg gacgcgttac cagacggaaa aaaatccaca ctatgtaata cggtcatact
     7981 ggcctcctga tgtcgtcaac acggcgaaat agtaatcacg aggtcaggtt cttaccttaa
     8041 attttcgacg gaaaaccacg taaaaaacgt cgatttttca agatacagcg tgaattttca
     8101 ggaaatgcgg tgagcatcac at
//
