LOCUS       sequence-334544-        9327 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Overexpresses TTYH2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9327)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_TTYH2_OE_1
FEATURES             Location/Qualifiers
     source          1..9327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        44..347
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        348..551
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     502..522
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    574..598
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2204..2228)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2230..2271
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2313..2901
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2366..2386)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2784..2795)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2973..3206
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3284..3405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3321..3340)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3375..3394
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3445..3580
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3600..3619)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3601..3619)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3629..3646)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3629..3645)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3638..3660)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3787..4242
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3874..3893)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4084..4105
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4268..4372
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4373..4441,4442..5233)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4591..4610)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5404..5992
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5893..5912
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6146..6163
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6280..6301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6316..6321,6322..6339,6340..6346)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6354..6370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6359..6381
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6378..6394
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6378..6394
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6413..6433
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6415..6433
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6461..6687
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6688..6868
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6915..7040
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7533..7766
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7951..7995
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8170..8670
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8589..8607
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8682..9077
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9137..9254
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
       61 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      121 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      181 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      241 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      301 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      361 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      421 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      481 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      541 taagcagagc tctctggcta actgtcggga tcaacaagtt tgtacaaaaa agttggcaca
      601 atgcaggcgg cgcgcgtgga ctacatcgct ccctggtggg tcgtgtggct gcacagcgtc
      661 ccgcacgtcg gcctgcgcct gcagcccgtg aacagcacct tcagccccgg cgacgagagt
      721 taccaggagt cgctgctgtt cctggggctg gtggccgccg tctgcctggg cctgaacctc
      781 atcttccttg tggcttacct ggtctgtgca tgccactgcc ggcgggacga tgcggtgcag
      841 accaagcagc accactcctg ctgcatcacc tggacggccg tggtggccgg gctcatctgc
      901 tgtgctgcgg tgggcgttgg tttctatgga aacagcgaga ccaacgatgg ggcgtaccag
      961 ctgatgtact ccttggacga tgccaaccac accttctctg ggatcgatgc tctggtttcc
     1021 ggaactaccc agaagatgaa ggtggaccta gagcagcacc tggcccggct cagtgagatc
     1081 tttgctgccc ggggcgatta cctgcagacc ctgaagttca tacagcagat ggcgggcagc
     1141 gttgttgttc agctctcagg actgcccgtg tggagggagg tcaccatgga gctgaccaag
     1201 ctatccgacc agactggcta cgtggagtac tacaggtggc tctcctacct cctgctcttt
     1261 atcctggacc tggtcatctg cctcattgcc tgcctgggac tggccaagcg ctccaagtgt
     1321 ctcctggcct cgatgctgtg ctgtggggca ctgagcctgc tcctcagttg ggcatccctg
     1381 gccgctgatg gctctgcggc agtggccacc agtgacttct gtgtggctcc tgacaccttc
     1441 atcctgaacg tcacggaggg ccagatcagc acagaggtga ctcgctacta cctgtattgc
     1501 agccagagtg gaagcagccc cttccagcag accctgacca ccttccagcg cgcacttacc
     1561 accatgcaga tccaggtcgc ggggctgctg cagtttgccg tgcccctctt ctccactgca
     1621 gaggaagacc tgcttgcaat ccagctcctg ctgaactcct cagagtccag ccttcaccag
     1681 ctgaccgcca tggtggactg ccgagggctg cacaaggatt atctggacgc tcttgctggc
     1741 atctgctacg acggcctcca gggcttgctg taccttggcc tcttctcctt cctggccgcc
     1801 ctcgccttct ccaccatgat ctgtgcaggg ccaagggcct ggaagcactt caccaccaga
     1861 aacagagact acgatgacat tgatgatgat gaccccttta acccccaagc ctggcgcatg
     1921 gcggctcaca gtcccccgag gggacagctt cacagcttct gcagctacag cagtggcctg
     1981 ggaagtcaga ccagcctgca gcccccggcc cagaccatct ccaacgcccc tgtctccgag
     2041 tacatgaacc aagccatgct ctttggtagg aacccacgct acgagaacgt gccactaatc
     2101 gggagagcct cccctccgcc tacgtactct cccagcatga gagccaccta cctgtctgtg
     2161 gcggatgagc acctgaggca ctacgggaat cagtttccag ccttgccaac tttcttgtac
     2221 aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     2281 gctagcgcta accggtggcg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     2341 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     2401 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     2461 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     2521 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     2581 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     2641 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     2701 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     2761 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     2821 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     2881 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     2941 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     3001 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     3061 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     3121 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     3181 ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca
     3241 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     3301 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3361 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     3421 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     3481 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     3541 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     3601 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     3661 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     3721 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     3781 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3841 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3901 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3961 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4021 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4081 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4141 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4201 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     4261 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4321 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4381 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4441 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4501 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4561 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4621 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4681 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4741 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4801 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4861 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4921 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4981 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5041 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5101 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5161 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5221 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5281 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5341 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5401 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5461 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5521 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5581 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5641 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5701 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5761 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5821 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5881 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5941 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6001 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6061 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6121 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6181 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     6241 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     6301 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     6361 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     6421 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
     6481 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     6541 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     6601 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     6661 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     6721 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6781 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6841 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     6901 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     6961 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     7021 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     7081 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     7141 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     7201 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     7261 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     7321 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     7381 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     7441 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     7501 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     7561 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     7621 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     7681 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     7741 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     7801 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     7861 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     7921 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     7981 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     8041 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     8101 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     8161 aggggaccct tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
     8221 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
     8281 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
     8341 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
     8401 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
     8461 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
     8521 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
     8581 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
     8641 gttgaccgaa tcaccgacct ctctccccag ggggtaccac catggccaag cctttgtctc
     8701 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg
     8761 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca
     8821 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg
     8881 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct
     8941 tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca
     9001 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg
     9061 gttatgtgtg ggagggcctg cagctgcagt agtaagaatt ctagatcttg agacaaatgg
     9121 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     9181 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     9241 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
     9301 agggggcccg gggttattaa tagtaat
//
