LOCUS       sequence-334568-        9276 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Overexpresses CHRND.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9276)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CHRND_OE_1
FEATURES             Location/Qualifiers
     source          1..9276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        36..339
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        340..543
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     494..514
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    566..590
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2145..2169)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2171..2212
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2254..2842
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2307..2327)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2725..2736)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2914..3147
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3225..3346
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3262..3281)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3316..3335
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3386..3521
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3541..3560)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3542..3560)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3570..3587)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3570..3586)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3579..3601)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3728..4183
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3815..3834)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4025..4046
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4209..4313
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4314..4382,4383..5174)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4532..4551)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5345..5933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5834..5853
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6087..6104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6221..6242
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6257..6262,6263..6280,6281..6287)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6295..6311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6300..6322
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6319..6335
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6319..6335
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6354..6374
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6356..6374
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6402..6628
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6629..6809
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6856..6981
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7474..7707
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7892..7936
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8111..8611
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8530..8548
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8623..9018
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9078..9195
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
       61 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      121 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      181 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      241 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      301 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      361 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      421 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      481 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      541 gctctctggc taactgtcgg gatcaacaag tttgtacaaa aaagttggca ccatggaggg
      601 gccagtgctg acactggggc tgctggctgc cctggcggtg tgtggcagct gggggctgaa
      661 cgaggaggag cggctgatcc ggcacctgtt tcaagagaag ggctacaaca aggagctccg
      721 gcccgtggca cacaaagagg agagtgtgga cgttgccctg gccctcacac tctccaacct
      781 catctccctg aaagaagttg aggagaccct cactaccaat gtgtggatag agcacggctg
      841 gacagacaac cggctgaagt ggaatgctga agaatttgga aacatcagtg tcctgcgcct
      901 ccccccggac atggtgtggc tcccagagat tgtgctggag aacaacaatg acggctcctt
      961 ccagatctcc tactcctgca acgtgcttgt ctaccactac ggcttcgtgt actggctgcc
     1021 acctgccatc ttccgctcct cctgccccat ctctgtcacc tatttcccct tcgactggca
     1081 gaactgctcc ctcaagttca gttccctcaa gtatacggcc aaagagatca ccctgagcct
     1141 gaaacaggat gccaaggaga accgcaccta ccccgtggag tggatcatca ttgatcctga
     1201 aggcttcaca gagaacgggg agtgggagat agtccaccgg ccggccaggg tcaacgtgga
     1261 ccccagagcc cctctggaca gccccagccg ccaggacatc accttctacc tcatcatccg
     1321 ccgcaagccc ctcttctaca tcatcaacat cctggtgccc tgcgtgctca tctccttcat
     1381 ggtcaacctg gtcttctacc taccggctga cagtggtgag aagacatcag tggccatctc
     1441 ggtgctcctg gctcagtctg tcttcctgct gctcatctcc aagcgtctgc ctgccacatc
     1501 catggccatc ccccttatcg gcaagttcct gctcttcggc atggtgctgg tcaccatggt
     1561 tgtggtgatc tgtgtcatcg tgctcaacat ccacttccga acacccagca cccatgtgct
     1621 gtctgagggg gtcaagaagc tcttcctgga gaccctgccg gagctcctgc acatgtcccg
     1681 cccagcagag gatggaccca gccctggggc cctggtgcgg aggagcagct ccctgggata
     1741 catctccaag gccgaggagt acttcctgct caagtcccgc agtgacctca tgttcgagaa
     1801 gcagtcagag cggcatgggc tggccaggcg cctcaccact gcacgccggc ccccagcaag
     1861 ctctgagcag gcccagcagg aactcttcaa tgagctgaag ccagctgtgg atggggcaaa
     1921 cttcattgtt aaccacatga gggaccagaa caattacaat gaggagaaag acagctggaa
     1981 ccgagtggcc cgcacagtgg accgcctctg cctgtttgtg gtgacgcctg tcatggtggt
     2041 gggcacagcc tggatcttcc tgcagggcgt ttacaaccag ccaccacccc agccttttcc
     2101 tggggacccc tactcctaca acgtgcagga caagcgcttc atcttgccaa ctttcttgta
     2161 caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg
     2221 agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     2281 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2341 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2401 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2461 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2521 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2581 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2641 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     2701 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2761 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2821 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     2881 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     2941 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     3001 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     3061 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3121 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     3181 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     3241 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3301 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     3361 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3421 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3481 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     3541 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     3601 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     3661 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     3721 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3781 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3841 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3901 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3961 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4021 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4081 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4141 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     4201 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4261 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4321 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4381 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4441 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4501 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4561 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4621 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4681 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4741 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4801 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4861 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4921 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4981 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5041 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5101 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5161 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5221 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5281 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5341 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5401 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5461 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5521 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5581 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5641 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5701 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5761 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5821 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5881 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5941 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6001 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6061 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6121 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6181 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6241 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6301 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     6361 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     6421 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     6481 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     6541 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     6601 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     6661 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6721 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6781 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     6841 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     6901 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     6961 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     7021 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7081 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7141 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7201 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7261 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     7321 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     7381 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     7441 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     7501 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     7561 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     7621 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     7681 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     7741 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     7801 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     7861 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     7921 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     7981 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     8041 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     8101 gaggggaccc ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
     8161 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
     8221 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
     8281 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
     8341 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
     8401 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
     8461 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
     8521 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
     8581 cgttgaccga atcaccgacc tctctcccca gggggtacca ccatggccaa gcctttgtct
     8641 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     8701 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     8761 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     8821 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     8881 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     8941 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     9001 ggttatgtgt gggagggcct gcagctgcag tagtaagaat tctagatctt gagacaaatg
     9061 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     9121 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     9181 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc
     9241 gagggggccc ggggttatta atagtaatca attacg
//
