LOCUS       sequence-334749-        9797 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Encodes the GwCas7x3 protein only for bacterial recombinant protein 
            expression for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Ozcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, 
            Koonin EV, Abudayyeh OO, Gootenberg JS
  TITLE     Programmable RNA targeting with the single-protein CRISPR effector 
            Cas7-11.
  JOURNAL   Nature. 2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5.
  PUBMED    34489594
REFERENCE   2  (bases 1 to 9797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5."
COMMENT     Sequence Label: pDF0163 GwCas7x3 bacterial expression with
            Twi...
FEATURES             Location/Qualifiers
     source          1..9797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      77..665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     566..585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     819..836
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    851..990
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(916..935)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1076..1098
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1092..1283)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2595..3677)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3639..3658
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3678..3755)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3961..3980
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4064..4083
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4064..4082
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4083..4107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4122..4144
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4163..4180
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4190..4207
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(4220..4243,4244..4279,4280..4303)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             4304..4597
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     terminator      8375..8422
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(8597..8602,8603..8619,8620..8625))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(8652..8670)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8738..8842
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8843..8911,8912..9703)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9061..9080)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      781 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      841 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      901 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
      961 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     1021 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     1081 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
     1141 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
     1201 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     1261 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
     1321 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
     1381 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
     1441 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag
     1501 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc
     1561 cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc
     1621 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc
     1681 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg
     1741 cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg
     1801 gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag
     1861 aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca
     1921 ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt
     1981 atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa
     2041 tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc
     2101 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg
     2161 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg
     2221 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
     2281 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
     2341 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
     2401 cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga
     2461 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt
     2521 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat
     2581 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     2641 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt
     2701 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
     2761 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
     2821 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc
     2881 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg
     2941 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga
     3001 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga
     3061 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc
     3121 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
     3181 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
     3241 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
     3301 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag
     3361 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
     3421 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
     3481 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
     3541 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
     3601 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
     3661 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc
     3721 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg
     3781 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
     3841 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     3901 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     3961 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     4021 gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata
     4081 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
     4141 gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg
     4201 cggcagccat atggctagct ggagccatcc gcagtttgaa aaaggtggtg gtagcggtgg
     4261 tggttcaggt ggtagtgcat ggtcacaccc tcagtttgag aaaatgtcgg actcagaagt
     4321 caatcaagaa gctaagccag aggtcaagcc agaagtcaag cctgagactc acatcaattt
     4381 aaaggtgtcc gatggatctt cagagatctt cttcaagatc aaaaagacca ctcctttaag
     4441 aaggctgatg gaagcgttcg ctaaaagaca gggtaaggaa atggactcct taagattctt
     4501 gtacgacggt attagaatcc aagctgatca gacccctgaa gatttggaca tggaggataa
     4561 cgatattatt gaggctcaca gagaacagat tggtggatcc atgagcaatc agacccgttg
     4621 gattattgaa ggcaccctgg aactgattac accgctgcat attggcaccg gtctggatga
     4681 aaaagaacgt gatgaaaata aagaaacccg ttggctggaa gcagttgcac tggatcataa
     4741 aggtcagccg tatattccgg gtgcaagcct gaaaggtgca ctgcgtgcac tggcaaaacg
     4801 tcatgatttt cgtaacctgt tcgataacaa agaagtggac ggcgattttg ttcgtcaggc
     4861 agaatttctg agcgcatggt gtgttccgga taccgataaa ggtcgtctga ttcagcctcg
     4921 tgttgcaatt gatcgtgtta ccggcaccgc acaggataaa aaactgtttc agacacgtct
     4981 gattactccg ggtacacgtt ttgcaatgaa aattgttgtt cagaacgccg tggaaaacga
     5041 aattgccgat ctgctgggtc tgctgaatct gctgccggat gatccgcagt ttagcctggg
     5101 tgcatatgcc aatcaaggtc agggtcgtgt tcagtggttt ggtaaaattc agacccgctg
     5161 ctttggtatt aatgaagcaa aagcatggta tgaagagatc cgcaaagatg aaagcaaatg
     5221 ttggaccgca tttgccaaac cgaaaaatgt tagcacccct ccgacaccgg caaaagaagc
     5281 acagctgacc ctgccgctga acctggcatt tcataccccg tttctggtta aacaggcagg
     5341 tattaaagca gatgatgcag atgcagttcc gcgtcgtacc catgatgata aaattgttct
     5401 gcctgcaagc agcctgcgtg gtcgtctgcg tacccagagc gaacgtattc tgcgcaccct
     5461 gggttgtgaa acaccgcagg gtcataccgc accggcatat cgtaaaggcc agccgcatga
     5521 tgatctggca gttctgctgt ttggtgcagc aggttggcgt ggtattgttc agaccagcga
     5581 ttgtattgtt gaggacaaaa gcattaaaac ccgtcgtcat gaaatgctgg ccattgatcg
     5641 ctttacaggt ggtggtaaag atggtgccaa attcaatgtt gattatgtgg aatgtccgac
     5701 gctggcaggt aaactgagcc tggatctggc acgtctgaaa aatgcaaaac tgaaaggcgg
     5761 aaaagatgca ctgctgcctg cactgggtct gatgaccctg atgctgcgtg atctggccga
     5821 aggtgatatt ccgtttggtt atggtattag caaaggttat ggtcagtgtc gtgccagcag
     5881 cgcattaggt gattgggctg aactgctgaa acagcatctg ggtgcagata gcgcagatac
     5941 caccgttcag gccctgcgtg aatatctggg taatccgaaa ggtcaagaac tgaaactgga
     6001 tcctccgagc gcagatgcaa cccaggcagg cgttccggca cagcagaatg cagcaaaaac
     6061 ccaggcgcag ggtgcacaag aaaaatttca taatccgtat cactttatcc cgctgagcaa
     6121 accggatatt agccagtggc ctgaaccgca gaaactgacc gaaaaaggtc atagccatga
     6181 tcgttatgcc agcctgagcg gtcgtattgt ttgtcgtctg accacacaga caccgctgtt
     6241 tattggtagc gaacagacca caccgaccaa tccgcaggca ccgaaaagcc tgcatccgtt
     6301 taaactgaat aatggtctgg caattccggc aacctcactg cgtggtatga ttagcagcct
     6361 gtttgaaagc gttagcaata gcaattttcg tgtgctggat gagaaaacct atagcatgcg
     6421 taaaaccatg cagcagagcc tgagtgcaat gggtcgcatt gtgcgtcatg atcagaaact
     6481 gtacctgctg cctctgacac tgccgacgct gcctcagggt ccgcatggcg tttatgatct
     6541 gggtgaaaaa tggtcagcag ttttcgattg gcagcctccg cctctgcgta tctattttga
     6601 tccgcctccg cgtcgcacct atcagagcca gcagccgtgt tatatgaaac tgagcaccgt
     6661 gaaatatagc gaaagcaatc cgaatcagat tatcgcaggc gaaaacctgg gtgccctgcg
     6721 ttttccgcgt ggtaatcaga atacccagtt cctgattggt cagtcaaatc aggatgaatg
     6781 cccgattaca caggccgaat atgcacagaa aagcgaagat gaacgtaacg aatataccag
     6841 cggttgggtt cgtaccctgg tgaaaccggg tcgtgatctg cctcgtagcg ttaaacatca
     6901 tgtttttctg ccggacgtgt ttattgatgc accgcctccg gttaatgatc tgtatccgat
     6961 tccggatagc gttattcagc gttttcacga tctggcggat caggttctgg caagcatgaa
     7021 tctgaaaccg gaagaaattg ttgatagcac caatctgctt ccgtatacac cggttggtcg
     7081 tcgtagcgat agcgattgtc gtgatacccg tctgcaagcc ggtgatctga tcttttttga
     7141 tattgatcct ccgctgcatc caggtgaaaa aagccagatt accgaaatta gctttagcag
     7201 catttggcgt agcggtattg gcaaagacca tctgctgaca acaccggatc tgcttaccaa
     7261 ttttgatgtt aatctgcagc ctcatggtat gcctggtcgc acccagagcc tgtcaccagc
     7321 cgaactgtta tttggtctgg ttggcaccca gaatgatcag gcaaccaccg catatgcagg
     7381 taaagttcgt attggttttg gtctgccgga agaaggccat aatccgcgtc tggatgcacg
     7441 tattaccctg aaagaactga gcagcccgaa accgcctagt ccggcactgt attttcgcaa
     7501 aaaaagcggt aaagacgagt atgtgagcaa agcaaatctg gcagataagc cggaagatta
     7561 tattctgcgt ggccgtaaaa tgtatctgca tgcatggcgt aaaaatgaac aggttgttga
     7621 actgagcgat accggtcatg atggtggtgt tcgtccgcct tgggttagca aatttgatga
     7681 aagtgcagat gaaggcaata aacgtcgtgt tagcattgaa ccgattgcaa aagatgagag
     7741 cttttatttc gaagtggatt tccataatct gagccgtacc gaactggccc agctgtgtgc
     7801 aaccctgtat cctaatgaaa aatttgaaca tcgtcttggc atgggtaaac cgttaggtct
     7861 gggtagtatc aaaattactc cgctgagcct gtttctggtg aatcgtagcc agcgttatgc
     7921 aaccgatggt ctggataaac cgcgttatca tgcagtttgg cataccggta cagcaagcga
     7981 accgcgttgg cctgatcact tacagcgtga acagcagggt attgcatttg aaggtgtgag
     8041 caccgcaccg accgttatga gcctggcagc cgaagccaaa gttagtgatg atgttaaacg
     8101 tgcgctggaa ctgctgggca atccggatga aattaccgtt ccggttcatt atccgcagct
     8161 gcataatggc ctgatggaaa gcaaacagtt tgattggttt gtgcagaacg ataaaagcgg
     8221 tcgcgatcag cctgcaaata atcgtcagca tctgagcagc tttaccaaag ataccgaaaa
     8281 actggaaccg ctgattcgta ttatgcgtcg ttaagcggcc gcactcgagg cccgaaagga
     8341 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     8401 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc ccgcaagagg
     8461 cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg
     8521 atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac
     8581 tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg
     8641 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     8701 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     8761 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     8821 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     8881 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     8941 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     9001 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     9061 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     9121 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     9181 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     9241 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     9301 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     9361 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     9421 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     9481 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     9541 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     9601 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     9661 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     9721 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     9781 gatccttttt gataatc
//
