LOCUS       sequence-334993-        5812 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express IRF7 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5812)
  TITLE     IKK-epsilon isoform switching governs the immune response against 
            EV71 infection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: F-IRF7
FEATURES             Location/Qualifiers
     source          1..5812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        57..414
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        57..248
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(80..100)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      265..400
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             449..1243
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             449..1243
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(503..522)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1113..1132
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1431..1450)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1475..1522
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1851..2439
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2340..2359
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2614..2917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2918..3121
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3072..3092
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        3167..3185
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      3220..3229
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3229..3252
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4788..4804)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4789..4805)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4860..4879)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4861..4879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5153..5274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5190..5209)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5244..5263
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5281..5736)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5418..5439)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5630..5649
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(5763..5812,1..55)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agaatcctga
       61 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
      181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      241 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
      301 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
      361 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
      421 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
      481 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
      541 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
      601 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
      661 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
      721 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
      781 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
      841 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
      901 tcttgtcgat caggatgatc tggacgaaga acatcagggg ctcgcgccag ccgaactgtt
      961 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     1021 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     1081 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     1141 acttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     1201 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     1261 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     1321 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     1381 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
     1441 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     1501 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     1561 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     1621 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     1681 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
     1741 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     1801 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1861 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1921 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1981 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2041 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2101 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2161 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2221 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2281 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2341 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2401 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2461 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2521 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
     2581 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2641 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2701 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2761 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2821 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2881 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2941 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     3001 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     3061 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     3121 tggtttagtg aaccgtcaga tccgctagcg attacgccaa gctcgaaatt aaccctcact
     3181 aaagggaaca aaagctggag ctccaccgcg gtggcggccg ccaccatgga ttacaaggat
     3241 gacgacgata agagcccggg cggatccatg gccttggctc ctgagagggc agccccacgc
     3301 gtgctgttcg gagagtggct ccttggagag atcagcagcg gctgctatga ggggctgcag
     3361 tggctggacg aggcccgcac ctgtttccgc gtgccctgga agcacttcgc gcgcaaggac
     3421 ctgagcgagg ccgacgcgcg catcttcaag gcctgggctg tggcccgcgg caggtggccg
     3481 cctagcagca ggggaggtgg cccgcccccc gaggctgaga ctgcggagcg cgccggctgg
     3541 aaaaccaact tccgctgcgc actgcgcagc acgcgtcgct tcgtgatgct gcgggataac
     3601 tcgggggacc cggccgaccc gcacaaggtg tacgcgctca gccgggagct gtgctggcga
     3661 gaaggcccag gcacggacca gactgaggca gaggcccccg cagctgtccc accaccacag
     3721 ggtgggcccc cagggccatt cctggcacac acacatgctg gactccaagc cccaggcccc
     3781 ctccctgccc cagctggtga caagggggac ctcctgctcc aggcagtgca acagagctgc
     3841 ctggcagacc atctgctgac agcgtcatgg ggggcagatc cagtcccaac caaggctcct
     3901 ggagagggac aagaagggct tcccctgact ggggcctgtg ctggaggccc agggctccct
     3961 gctggggagc tgtacgggtg ggcagtagag acgaccccca gccccgggcc ccagcccgcg
     4021 gcactaacga caggcgaggc cgcggcccca gagtccccgc accaggcaga gccgtacctg
     4081 tcaccctccc caagcgcctg caccgcggtg caagagccca gcccaggggc gctggacgtg
     4141 accatcatgt acaagggccg cacggtgctg cagaaggtgg tgggacaccc gagctgcacg
     4201 ttcctatacg gccccccaga cccagctgtc cgggccacag acccccagca ggtagcattc
     4261 cccagccctg ccgagctccc ggaccagaag cagctgcgct acacggagga actgctgcgg
     4321 cacgtggccc ctgggttgca cctggagctt cgggggccac agctgtgggc ccggcgcatg
     4381 ggcaagtgca aggtgtactg ggaggtgggc ggacccccag gctccgccag cccctccacc
     4441 ccagcctgcc tgctgcctcg gaactgtgac acccccatct tcgacttcag agtcttcttc
     4501 caagagctgg tggaattccg ggcacggcag cgccgtggct ccccacgcta taccatctac
     4561 ctgggcttcg ggcaggacct gtcagctggg aggcccaagg agaagagcct ggtcctggtg
     4621 aagctggaac cctggctgtg ccgagtgcac ctagagggca cgcagcgtga gggtgtgtct
     4681 tccctggata gcagcagcct cagcctctgc ctgtccagcg ccaacagcct ctatgacgac
     4741 atcgagtgct tccttatgga gctggagcag cccgcctaga agcttatcga taccgtcgac
     4801 ctcgaggggg ggcccggtac cttaattaat taaggtacca ggtaagtgta cccaattcgc
     4861 cctatagtga gtcgtattac aattcactcg atcgcccttc ccaacagttg cgcagcctga
     4921 atggcgaatg gagatccaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     4981 ttgtgtattt tagattcaca gtcccaaggc tcatttcagg cccctcagtc ctcacagtct
     5041 gttcatgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     5101 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     5161 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5221 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt
     5281 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     5341 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     5401 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     5461 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     5521 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     5581 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     5641 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     5701 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     5761 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat cc
//
