LOCUS       sequence-335047-        9890 bp    DNA     circular SYN 14-APR-2025
DEFINITION  For bacterial expression of AsCas12a R1226A (RuvC-inhibiting 
            mutation) with an N-terminal His-MBP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Cofsky JC, Karandur D, Huang CJ, Witte IP, Kuriyan J, Doudna JA
  TITLE     CRISPR-Cas12a exploits R-loop asymmetry to form double-strand 
            breaks.
  JOURNAL   Elife. 2020 Jun 10;9. pii: 55143. doi: 10.7554/eLife.55143.
  PUBMED    32519675
REFERENCE   2  (bases 1 to 9890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-06-10"; volume: "9. pii: 55143. doi"; pages: " 
            10.7554/eLife.55143"
COMMENT     Sequence Label: pJCC_076 AsCas12a R1226A
FEATURES             Location/Qualifiers
     source          1..9890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(28..33,34..50,51..56))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(83..101)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        169..273
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(274..342,343..1134)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(492..511)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1305..1893
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1794..1813
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2047..2064
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2079..2218
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2144..2163)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2304..2326
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2320..2511)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     3969..3988
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4072..4091
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4072..4090
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4179..4201
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4221..4250
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             4260..5360
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     5334..5357
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             5415..5435
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(9491..9509)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9505..9552
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat
       61 gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      121 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      181 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      241 taaatgcttc aataacattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      301 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      361 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      421 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
      481 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
      541 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
      601 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
      661 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
      721 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
      781 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
      841 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
      901 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
      961 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     1021 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1081 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1141 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1201 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1261 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1321 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1381 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1441 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1501 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1561 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1621 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1681 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1741 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1801 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1861 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1921 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1981 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2041 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     2101 acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg
     2161 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     2221 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     2281 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     2341 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca
     2401 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg
     2461 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc
     2521 cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct
     2581 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca
     2641 actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt
     2701 cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg
     2761 gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc
     2821 gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
     2881 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
     2941 ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga
     3001 gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt
     3061 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
     3121 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
     3181 cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg
     3241 ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa
     3301 gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
     3361 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     3421 ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg
     3481 tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
     3541 gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
     3601 atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
     3661 tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc
     3721 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct
     3781 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
     3841 gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
     3901 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     3961 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     4021 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
     4081 tcactatagg gagaccacaa cggtttccct ctagtgccgg ctccggagag ctctttaatt
     4141 aagcggccgc cctgcaggac tcgagttcta gaaataattt tgtttaactt taagaaggag
     4201 atatacatat gaaatcttct caccatcacc atcaccatca ccatcaccat ggttcttcta
     4261 tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc
     4321 tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc
     4381 cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta
     4441 tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca
     4501 ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca
     4561 acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag
     4621 atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga
     4681 aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc
     4741 tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag
     4801 acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta
     4861 aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag
     4921 gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag
     4981 tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg
     5041 gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc
     5101 tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg
     5161 gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca
     5221 ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct
     5281 ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag
     5341 ccctgaaaga cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca
     5401 acctcgggat cgaggaaaac ctgtacttcc aatccaatgc aatgacccag ttcgaggggt
     5461 ttaccaatct gtatcaagtg agcaagacgc tgcgctttga actgatccca cagggaaaaa
     5521 ccttaaaaca tattcaagag cagggcttta tcgaagaaga taaggcccgt aatgaccatt
     5581 acaaagagtt aaagccgatt attgatcgta tctacaagac ctatgcggac cagtgcttac
     5641 aattggtaca gcttgattgg gagaacctct ctgccgccat cgattcctat cgtaaagaaa
     5701 aaactgaaga aacgcgcaac gccctgattg aagagcaggc cacctatcgt aacgcgattc
     5761 atgactattt tattggccgt acggacaatc tgacggacgc gatcaacaag cgccatgcgg
     5821 agatttacaa aggactgttt aaggctgaac tgttcaatgg taaggtcctt aaacagcttg
     5881 ggaccgtcac aacgacggaa catgaaaacg cgttattacg tagcttcgac aagtttacca
     5941 cgtatttctc cggcttttac gaaaatcgca aaaacgtttt cagtgccgag gatatttcca
     6001 ctgctatccc tcatcgcatt gtgcaagaca acttcccaaa attcaaagaa aattgtcata
     6061 tcttcacccg cttaatcacc gctgtaccgt ccctgcgtga gcatttcgaa aacgtgaaaa
     6121 aggccattgg tatcttcgtg tctacttcga ttgaggaggt attttccttt ccattctata
     6181 atcagctgct gacccagacc caaattgatc tgtacaacca gctgcttggc ggtatttctc
     6241 gtgaagcagg aaccgaaaaa atcaaagggt tgaacgaggt gcttaatctg gcaatccaga
     6301 aaaatgatga aaccgcccac atcattgctt cgttacctca tcgttttatc ccgttgttca
     6361 agcaaatttt aagtgatcgc aatacgctgt cgtttattct ggaagaattc aaaagtgatg
     6421 aagaggtaat tcagtcgttt tgcaaatata aaaccctgtt acgtaacgaa aatgtcctgg
     6481 aaacagccga ggctttgttt aacgaactga atagcattga cctgacgcat atctttatta
     6541 gccacaaaaa attagagacc atctcatcag ctctgtgcga tcattgggat acactgcgca
     6601 atgcgctgta tgaacgtcgt atttcggaat tgactggcaa aatcactaaa agcgcgaaag
     6661 agaaagtaca gcgctcgctt aaacatgaag atatcaacct gcaggagatc atcagcgccg
     6721 cgggtaaaga actgtcggag gcatttaaac agaagacgag cgagattctg tcccacgcac
     6781 atgccgcctt agaccagccg ctcccgacca ctctgaagaa acaggaagag aaagaaatcc
     6841 ttaaaagtca actggacagt ttactgggtc tctatcatct gctggattgg tttgcggtag
     6901 acgaaagcaa tgaagtggat ccggagttta gtgcccgtct gacaggaatc aagctggaaa
     6961 tggagccttc gcttagcttc tacaacaaag cccgcaatta tgccacgaaa aaaccctata
     7021 gtgtcgaaaa atttaaactc aactttcaaa tgccgaccct tgcgtcgggc tgggatgtca
     7081 acaaagaaaa aaacaacgga gctattctgt tcgttaaaaa tggtctgtac tacctgggca
     7141 tcatgccgaa acagaaaggt cgctacaaag ccctttcgtt cgagcccacg gaaaaaacaa
     7201 gcgaaggctt cgacaaaatg tactacgatt actttccgga tgcagcaaaa atgatcccga
     7261 aatgttccac acagctgaaa gccgttacag cacattttca gacgcacacc acccccatct
     7321 tactgtccaa caattttatt gaaccgctgg agattactaa agaaatttat gatttgaaca
     7381 atccggaaaa agagccaaaa aagtttcaaa ccgcctacgc taaaaaaacc ggggatcaga
     7441 aagggtaccg cgaagcgttg tgcaagtgga ttgatttcac ccgcgatttt ctcagtaaat
     7501 ataccaagac tacctcgatt gacctgagct cactgcgccc gagctctcaa tataaggatt
     7561 tgggtgagta ctatgctgaa ttaaaccctt tattgtacca catttctttt cagcgcatcg
     7621 ccgaaaagga aattatggac gcagtcgaaa ccgggaaact gtacctgttc cagatctata
     7681 ataaggactt cgccaaagga catcatggca aaccgaacct gcacaccctt tactggaccg
     7741 ggcttttctc tccggaaaat ttggcgaaaa cctcgatcaa gcttaacggt caagctgagc
     7801 tgttttaccg tccaaaatcc cgcatgaagc gcatggcgca tcgtttaggt gaaaaaatgc
     7861 tgaataagaa actgaaagat cagaaaaccc ctatcccgga taccctctac caggaactgt
     7921 atgattacgt gaaccatcgt ctctcgcatg acctgtcaga cgaagcgcgt gcgttactgc
     7981 ccaatgtaat cacaaaagaa gtttcgcatg aaattattaa agatcgtcgt tttacatctg
     8041 ataaattctt ttttcatgtt ccgatcaccc tcaactatca ggccgcaaac agtccaagta
     8101 agtttaacca gcgcgttaat gcttacctga aggaacatcc ggagactccg attattggaa
     8161 ttgatcgcgg tgaacgtaat ttgatctata tcactgtgat cgatagtacc ggtaagattc
     8221 tggagcagcg cagcttgaac acaattcaac agtttgatta tcagaaaaaa ttagacaacc
     8281 gcgaaaaaga gcgcgtggct gcccgtcagg cgtggtctgt tgtcggtacc attaaagatc
     8341 tgaagcaggg ctatctttct caggttattc acgaaattgt agatctgatg atccattatc
     8401 aggcggttgt tgtgttggag aatctcaatt tcggttttaa gagtaagcgc acaggcatcg
     8461 ctgaaaaagc agtttatcag cagtttgaaa aaatgctgat cgacaaattg aactgtttag
     8521 ttctcaaaga ttacccagcg gaaaaggtgg gcggagtgct gaatccgtac caattaacgg
     8581 atcaattcac ttccttcgca aagatgggta cccaaagcgg ctttctgttc tatgtgccgg
     8641 ccccgtatac ctcgaaaatc gatccactga cgggcttcgt agatccgttc gtgtggaaaa
     8701 ccattaaaaa tcatgaaagt cgtaaacatt ttctcgaagg cttcgacttc ctgcactacg
     8761 acgtgaaaac tggcgatttc attctgcatt ttaaaatgaa ccgcaacctt tcgtttcagc
     8821 gcggtctgcc gggctttatg ccggcttggg acattgtttt tgagaaaaat gaaacccagt
     8881 ttgatgctaa aggcactcct ttcatcgccg gtaaacgcat cgtacctgtg attgaaaacc
     8941 atcgttttac agggcgttac cgtgatttat acccggcgaa cgaattgatc gcgctgctgg
     9001 aggaaaaggg catcgttttc cgtgacggct ccaatattct gccgaaatta ctggaaaacg
     9061 acgattcaca cgcaattgat accatggtcg cactgattcg ctcagtctta cagatggcta
     9121 actctaatgc agccacagga gaagattata ttaattcgcc agtccgcgat ttgaacggtg
     9181 tttgcttcga cagccgtttt cagaatcctg aatggccgat ggacgctgat gccaacggag
     9241 cttatcatat cgccctgaaa ggccagctcc tgctgaacca cctgaaggaa agcaaagatc
     9301 tgaaattgca gaacggcatt agcaaccagg actggttagc atacatccag gaactgcgta
     9361 actaataaca ttggaagtgg ataacggatc cgcgatcgcg gcgcgccacc tggtggccgg
     9421 ccggtaccac gcgtgcgcgc tgatccggct gctaacaaag cccgaaagga agctgagttg
     9481 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     9541 aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc
     9601 catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc
     9661 aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag
     9721 cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag
     9781 gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag
     9841 gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
//
