LOCUS       sequence-335098-        3727 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Drosophila TP10 poly(a) terminator element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pTP10
FEATURES             Location/Qualifiers
     source          1..3727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(77..96)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(78..96)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(103..120)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(103..119)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(112..134)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      260..715
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(347..366)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     557..578
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        793..897
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(898..966,967..1758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1116..1135)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1929..2517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2418..2437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2671..2688
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2805..2826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2841..2846,2847..2864,2865..2871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2879..2895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2884..2906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2903..2919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2903..2919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2937..2955
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2937..2954
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cgagaccact catcgcccaa tcgaattccc gcggccgcca tggcggccgg gagcatgcga
       61 cgtcgggccc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
      121 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      181 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      241 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      301 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      361 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      421 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      481 gatggttcac gtagtgggcc agcgccctga tagacggttt ttcgcccttt gacgttggag
      541 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
      601 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
      661 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga
      721 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg tggcactttt
      781 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      841 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      901 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      961 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1021 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1081 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1141 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1201 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1261 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1321 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1381 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1441 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1501 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1561 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcgcgc
     1621 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1681 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1741 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     1801 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1861 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1921 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1981 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2041 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2101 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2161 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2221 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2281 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2341 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2401 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2461 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2521 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2581 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2641 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     2701 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     2761 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     2821 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     2881 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctattt
     2941 aggtgacact atagaatact caagctatgc atccaacgcg ttgggagctc tcccatatgg
     3001 tcgacctgca ggcggccgcg aattcactag tgattgggcg atgagtggtc tcggcttatg
     3061 aatcgttttt aaaataacaa atcaattgtt ttataatatt cgtacgattc tttgattatg
     3121 taataaaatg tgatcattag gaagattacg aaaaatataa aaaatatgag ttctgtgtgt
     3181 ataacaaatg ctgtaaacgc cacaattgtg tttgttgcaa ataaacccat gattatttga
     3241 ttaaaattgt tgttttcttt gttcatagac aatagtgtgt tttgcctaaa cgtgtactgc
     3301 ataaactcca tgcgagtgta tagcgagcta gtggctaacg cttgccccac caaagtagat
     3361 tcgtcaaaat cctcaatttc atcaccctcc tccaagttta acatttggcc gtcggaatta
     3421 acttctaaag atgccacata atctaataaa tgaaatagag attcaaacgt ggcgtcatcg
     3481 tccgtttcga ccatttccga aaagaactcg ggcataaact ctatgatttc tctggacgtg
     3541 gtgttgtcga aactctcaaa gtacgcagtc aggaacgtgc gcgacatgtc gtcgggaaac
     3601 tcgcgcggaa acatgttgtt gtaaccgaac gggtcccata gcgccaaaac caaatctgcc
     3661 agcgtcaata gaatgagcac gatgccgaca atggagctgg cttggatagc gattcgagtt
     3721 aaccagt
//
