LOCUS       sequence-335765-       11510 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway destination clone of PAK6 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11510)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-PAK6_IDG-K
FEATURES             Location/Qualifiers
     source          1..11510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    137..253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    287..307
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        307..1518
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1355..1374
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1522..1542)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1543..1552
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1555..2325
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2332..2373
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2374..2398
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4451..4475)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4505..5093
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4558..4578)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4976..4987)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5107..5606
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5142..5165)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5539..5558
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5627..6226
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5627..5646)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6123..6143
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6364..6597
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6669..6790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6706..6725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6760..6779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6830..6965
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6985..7004)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6986..7004)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7014..7031)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7014..7030)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7023..7045)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7172..7627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7259..7278)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7469..7490
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7653..7757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7758..7826,7827..8618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8789..9377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9278..9297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9531..9548
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9665..9686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9701..9706,9707..9724,9725..9731)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9739..9755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9744..9766
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9763..9779
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9763..9779
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9798..9818
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9800..9818
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9846..10072
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10073..10253
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10300..10425
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10918..11151
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11336..11380
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
       61 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
      121 gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
      181 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      241 aattcaaaat tttatcgtcc agtgtggtgg aattctgcag atatcacaac tttgtataga
      301 aaagttggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga
      361 gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca
      421 ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca
      481 ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag
      541 gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac
      601 gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat
      661 ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc
      721 tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga
      781 gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg
      841 ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg
      901 ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga
      961 ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg
     1021 gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc
     1081 ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt
     1141 cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta
     1201 ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc
     1261 ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt
     1321 tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc
     1381 tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt
     1441 gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt
     1501 ttggcttttt tgttagacgg ccaacttttg tatacaaagt gggccaccat gggaatcccg
     1561 ctgctgaacg ctaaacagat tctgggacag ctggacggcg ggagcgtggc agtcctgcct
     1621 gtggtcgact ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa gagtggggat
     1681 gcttgcattg cagaatatca gcaggcaggg agaggaagca gagggaggaa atggttctct
     1741 ccttttggag ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg accagcagca
     1801 atcggcctgg gcccggtcat cggaattgtc atggcagaag cgctgcgaaa gctgggagca
     1861 gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa gctggcaggc
     1921 atcctggtgg agctggccgg aataacaggc gatgctgcac agatcgtcat tggcgccggg
     1981 attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca atcagggctg gatcacactg
     2041 caggaagcag ggattaacct ggacaggaat actctggccg ctatgctgat ccgagagctg
     2101 cgggcagccc tggaactgtt cgagcaggaa ggcctggctc catatctgtc acggtgggag
     2161 aagctggata acttcatcaa tagacccgtg aagctgatca ttggggacaa agagattttc
     2221 gggattagcc gggggattga taaacaggga gccctgctgc tggaacagga cggagttatc
     2281 aaaccctgga tgggcggaga aatcagtctg cggtctgccg aaaaggctag cggcaagccc
     2341 atccccaacc ccctgctggg cctggacagc accacaagtt tgtacaaaaa agcaggctcc
     2401 accatgttcc gcaagaaaaa gaagaaacgc cctgagatct cagcgccaca gaacttccag
     2461 caccgtgtcc acacctcctt cgaccccaaa gaaggcaagt ttgtgggcct ccccccacaa
     2521 tggcagaaca tcctggacac actgcggcgc cccaagcccg tggtggaccc ttcgcgaatc
     2581 acacgggtgc agctccagcc catgaagaca gtggtgcggg gcagcgcgat gcctgtggat
     2641 ggctacatct cggggctgct caacgacatc cagaagttgt cagtcatcag ctccaacacc
     2701 ctgcgtggcc gcagccccac cagccggcgg cgggcacagt ccctggggct gctgggggat
     2761 gagcactggg ccaccgaccc agacatgtac ctccagagcc cccagtctga gcgcactgac
     2821 ccccacggcc tctacctcag ctgcaacggg ggcacaccag caggccacaa gcagatgccg
     2881 tggcccgagc cacagagccc acgggtcctg cccaatgggc tggctgcaaa ggcacagtcc
     2941 ctgggccccg ccgagtttca gggtgcctcg cagcgctgtc tgcagctggg tgcctgcctg
     3001 cagagctccc caccaggagc ctcgcccccc acgggcacca ataggcatgg aatgaaggct
     3061 gccaagcatg gctctgagga ggcccggcca cagtcctgcc tggtgggctc agccacaggc
     3121 aggccaggtg gggaaggcag ccctagccct aagacccggg agagcagcct gaagcgcagg
     3181 ctattccgaa gcatgttcct gtccactgct gccacagccc ctccaagcag cagcaagcca
     3241 ggccctccac cacagagcaa gcccaactcc tctttccgac cgccgcagaa agacaacccc
     3301 ccaagcctgg tggccaaggc ccagtccttg ccctcggacc agccggtggg gaccttcagc
     3361 cctctgacca cttcggatac cagcagcccc cagaagtccc tccgcacagc cccggccaca
     3421 ggccagcttc caggccggtc ttccccagcg ggatcccccc gcacctggca cgcccagatc
     3481 agcaccagca acctgtacct gccccaggac cccacggttg ccaagggtgc cctggctggt
     3541 gaggacacag gtgttgtgac acatgagcag ttcaaggctg cgctcaggat ggtggtggac
     3601 cagggtgacc cccggctgct gctggacagc tacgtgaaga ttggcgaggg ctccaccggc
     3661 atcgtctgct tggcccggga gaagcactcg ggccgccagg tggccgtcaa gatgatggac
     3721 ctcaggaagc agcagcgcag ggagctgctc ttcaacgagg tggtgatcat gcgggactac
     3781 cagcacttca acgtggtgga gatgtacaag agctacctgg tgggcgagga gctgtgggtg
     3841 ctcatggagt tcctgcaggg aggagccctc acagacatcg tctcccaagt caggctgaat
     3901 gaggagcaga ttgccactgt gtgtgaggct gtgctgcagg ccctggccta cctgcatgct
     3961 cagggtgtca tccaccggga catcaagagt gactccatcc tgctgaccct cgatggcagg
     4021 gtgaagctct cggacttcgg attctgtgct cagatcagca aagacgtccc taagaggaag
     4081 tccctggtgg gaacccccta ctggatggct cctgaagtga tctccaggtc tttgtatgcc
     4141 actgaggtgg atatctggtc tctgggcatc atggtgattg agatggtaga tggggagcca
     4201 ccgtacttca gtgactcccc agtgcaagcc atgaagaggc tccgggacag ccccccaccc
     4261 aagctgaaaa actctcacaa ggtctcccca gtgctgcgag acttcctgga gcggatgctg
     4321 gtgcgggacc cccaagagag agccacagcc caggagctcc tagaccaccc cttcctgctg
     4381 cagacagggc tacctgagtg cctggtgccc ctgatccagc tctaccgaaa gcagacctcc
     4441 acctgctagg acccagcttt cttgtacaaa gtggtgatat ccagcacagt ggcggccgct
     4501 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4561 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4621 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4681 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4741 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4801 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4861 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     4921 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4981 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5041 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     5101 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     5161 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     5221 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     5281 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     5341 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     5401 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     5461 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     5521 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     5581 cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa
     5641 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     5701 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     5761 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5821 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5881 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5941 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6001 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6061 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     6121 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6181 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
     6241 cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc ccggtacctt
     6301 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6361 gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     6421 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6481 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6541 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6601 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6661 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6721 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6781 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     6841 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6901 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6961 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     7021 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     7081 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     7141 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     7201 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7261 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7321 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7381 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7441 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7501 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7561 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     7621 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7681 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7741 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7801 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7861 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7921 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7981 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     8041 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8101 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8161 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8221 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8281 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8341 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8401 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8461 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8521 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8581 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8641 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8701 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8761 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8821 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8881 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8941 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9001 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9061 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9121 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9181 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9241 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9301 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9361 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9421 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9481 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9541 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9601 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     9661 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     9721 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     9781 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     9841 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     9901 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     9961 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
    10021 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
    10081 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    10141 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    10201 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
    10261 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
    10321 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    10381 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
    10441 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
    10501 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    10561 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    10621 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    10681 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    10741 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
    10801 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
    10861 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
    10921 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
    10981 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
    11041 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
    11101 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
    11161 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
    11221 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
    11281 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
    11341 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
    11401 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
    11461 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
//
