LOCUS       sequence-335766-       10730 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway destination clone of PIP4K2C (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10730)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-PIP4K2C_IDG-K
FEATURES             Location/Qualifiers
     source          1..10730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    87..203
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    237..257
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        257..1468
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1305..1324
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1472..1492)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1493..1502
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1505..2275
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2282..2323
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2324..2348
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3621..3645)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3675..4263
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3728..3748)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4146..4157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4277..4776
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4312..4335)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4709..4728
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4797..5396
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4797..4816)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5293..5313
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5534..5767
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5839..5960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5876..5895)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5930..5949
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6000..6135
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6155..6174)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6156..6174)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6184..6201)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6184..6200)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6193..6215)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6342..6797
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6429..6448)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6639..6660
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6823..6927
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6928..6996,6997..7788)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7959..8547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8448..8467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8701..8718
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8835..8856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8871..8876,8877..8894,8895..8901)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8909..8925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8914..8936
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8933..8949
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8933..8949
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8968..8988
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8970..8988
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9016..9242
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9243..9423
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9470..9595
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10088..10321
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10506..10550
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
       61 aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt
      121 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      181 caaattacaa aattcaaaat tttatcgtcc agtgtggtgg aattctgcag atatcacaac
      241 tttgtataga aaagttggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
      301 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
      361 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
      421 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
      481 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
      541 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
      601 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
      661 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
      721 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
      781 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
      841 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
      901 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
      961 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     1021 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
     1081 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca
     1141 cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg
     1201 gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg
     1261 tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg
     1321 aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat
     1381 gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt
     1441 ctggccgttt ttggcttttt tgttagacgg ccaacttttg tatacaaagt gggccaccat
     1501 gggaatcccg ctgctgaacg ctaaacagat tctgggacag ctggacggcg ggagcgtggc
     1561 agtcctgcct gtggtcgact ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa
     1621 gagtggggat gcttgcattg cagaatatca gcaggcaggg agaggaagca gagggaggaa
     1681 atggttctct ccttttggag ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg
     1741 accagcagca atcggcctgg gcccggtcat cggaattgtc atggcagaag cgctgcgaaa
     1801 gctgggagca gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa
     1861 gctggcaggc atcctggtgg agctggccgg aataacaggc gatgctgcac agatcgtcat
     1921 tggcgccggg attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca atcagggctg
     1981 gatcacactg caggaagcag ggattaacct ggacaggaat actctggccg ctatgctgat
     2041 ccgagagctg cgggcagccc tggaactgtt cgagcaggaa ggcctggctc catatctgtc
     2101 acggtgggag aagctggata acttcatcaa tagacccgtg aagctgatca ttggggacaa
     2161 agagattttc gggattagcc gggggattga taaacaggga gccctgctgc tggaacagga
     2221 cggagttatc aaaccctgga tgggcggaga aatcagtctg cggtctgccg aaaaggctag
     2281 cggcaagccc atccccaacc ccctgctggg cctggacagc accacaagtt tgtacaaaaa
     2341 agcaggctcc accatggcgt cctcctcggt cccaccagcc acggtatcgg cggcgacagc
     2401 aggccccggc ccaggtttcg gcttcgcctc caagaccaag aagaagcatt tcgtgcagca
     2461 gaaggtgaag gtgttccggg cggccgaccc gctggtgggt gtgttcctgt ggggcgtagc
     2521 ccactcgatc aatgagctca gccaggtgcc tcccccggtg atgctgctgc cagatgactt
     2581 taaggccagc tccaagatca aggtcaacaa tcaccttttc cacagggaaa atctgcccag
     2641 tcatttcaag ttcaaggagt attgtcccca ggtcttcagg aacctccgtg atcgatttgg
     2701 cattgatgac caagattact tggtgtccct tacccgaaac ccccccagcg aaagtgaagg
     2761 cagtgatggt cgcttcctta tctcctacga tcggactctg gtcatcaaaa aagtatccag
     2821 tgaggacatt gctgacatgc atagcaacct ctccaactat caccagtaca ttgtgaagtg
     2881 ccatggcaac acgcttctgc cccagttcct ggggatgtac cgagtcagtg tggacaacga
     2941 agacagctac atgcttgtga tgcgcaatat gtttagccac cgtcttcctg tgcacaggaa
     3001 gtatgacctc aagggttccc tagtgtcccg ggaagccagc gataaggaaa aggttaaaga
     3061 attgcccacc cttagggata tggactttct caacaagaac cagaaagtat atattggtga
     3121 agaggagaag aaaatatttc tggagaagct gaagagagat gtggagtttc tagtgcagct
     3181 gaagatcatg gactacagcc ttctgctagg catccacgac atcattcggg gctctgaacc
     3241 agaggaggaa gcgcccgtgc gggaggatga gtcagaggtg gatggggact gcagcctgac
     3301 tggacctcct gctctggtgg gctcctatgg cacctcccca gagggtatcg gaggctacat
     3361 ccattcccat cggcccctgg gcccaggaga gtttgagtcc ttcattgatg tctatgccat
     3421 ccggagtgct gaaggagccc cccagaagga ggtctacttc atgggcctca ttgatatcct
     3481 tacacagtat gatgccaaga agaaagcagc tcatgcagcc aaaactgtca agcatggggc
     3541 tggggcagag atctctactg tccatccgga gcagtatgct aagcgattcc tggattttat
     3601 taccaacatc tttgcctagg acccagcttt cttgtacaaa gtggtgatat ccagcacagt
     3661 ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3721 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3781 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3841 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3901 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3961 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4021 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     4081 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4141 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4201 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4261 cgcctggaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     4321 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     4381 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     4441 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     4501 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     4561 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     4621 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     4681 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     4741 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga
     4801 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     4861 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     4921 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     4981 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     5041 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     5101 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     5161 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     5221 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     5281 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     5341 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     5401 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc
     5461 ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5521 gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct
     5581 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5641 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5701 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5761 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     5821 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5881 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5941 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     6001 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6061 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     6121 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     6181 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     6241 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     6301 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     6361 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6421 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6481 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6541 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6601 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6661 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6721 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6781 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6841 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6901 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6961 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7021 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7081 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7141 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7201 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7261 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7321 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7381 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7441 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7501 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7561 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7621 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7681 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7741 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7801 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7861 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7921 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7981 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8041 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8101 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8161 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8221 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8281 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8341 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8401 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8461 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8521 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8581 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8641 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8701 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8761 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     8821 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     8881 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     8941 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     9001 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     9061 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     9121 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     9181 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     9241 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     9301 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     9361 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     9421 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     9481 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     9541 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     9601 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     9661 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     9721 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     9781 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     9841 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     9901 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     9961 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
    10021 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
    10081 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
    10141 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
    10201 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
    10261 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
    10321 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
    10381 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
    10441 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
    10501 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
    10561 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
    10621 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
    10681 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
//
