LOCUS       sequence-335769-       10664 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway destination clone of PRKACB (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10664)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-PRKACB_IDG-K
FEATURES             Location/Qualifiers
     source          1..10664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        53..157
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(158..226,227..1018)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1189..1777
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1678..1697
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1931..1948
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2065..2086
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2101..2106,2107..2124,2125..2131)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2139..2155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2144..2166
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2163..2179
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2163..2179
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2198..2218
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2200..2218
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2246..2472
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2473..2653
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2700..2825
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3318..3551
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3736..3780
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4047..4163
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4197..4217
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4217..5428
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5265..5284
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5432..5452)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5453..5462
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5465..6235
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6242..6283
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6284..6308
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7515..7539)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7569..8157
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7622..7642)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8040..8051)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8171..8670
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8206..8229)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8603..8622
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8691..9290
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8691..8710)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9187..9207
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9428..9661
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9733..9854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9770..9789)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9824..9843
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9894..10029
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10049..10068)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10050..10068)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10078..10095)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10078..10094)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10087..10109)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10236..10664
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10323..10342)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10533..10554
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
       61 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      121 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      181 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      241 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      301 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      361 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      421 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      481 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      541 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
      601 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
      661 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
      721 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
      781 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
      841 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
      901 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
      961 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1021 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1081 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1141 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1201 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1261 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1321 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1381 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1441 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1501 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1561 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1621 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1681 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1741 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1801 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1861 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     1921 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     1981 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     2041 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     2101 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     2161 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     2221 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     2281 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     2341 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     2401 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     2461 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     2521 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     2581 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     2641 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     2701 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     2761 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     2821 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     2881 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     2941 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     3001 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     3061 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     3121 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     3181 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     3241 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     3301 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     3361 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     3421 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     3481 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     3541 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     3601 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     3661 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     3721 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     3781 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     3841 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     3901 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     3961 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     4021 aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt
     4081 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     4141 caaattacaa aattcaaaat tttatcgtcc agtgtggtgg aattctgcag atatcacaac
     4201 tttgtataga aaagttggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     4261 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
     4321 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     4381 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     4441 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     4501 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     4561 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
     4621 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
     4681 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
     4741 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
     4801 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
     4861 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
     4921 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     4981 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
     5041 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca
     5101 cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg
     5161 gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg
     5221 tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg
     5281 aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat
     5341 gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt
     5401 ctggccgttt ttggcttttt tgttagacgg ccaacttttg tatacaaagt gggccaccat
     5461 gggaatcccg ctgctgaacg ctaaacagat tctgggacag ctggacggcg ggagcgtggc
     5521 agtcctgcct gtggtcgact ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa
     5581 gagtggggat gcttgcattg cagaatatca gcaggcaggg agaggaagca gagggaggaa
     5641 atggttctct ccttttggag ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg
     5701 accagcagca atcggcctgg gcccggtcat cggaattgtc atggcagaag cgctgcgaaa
     5761 gctgggagca gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa
     5821 gctggcaggc atcctggtgg agctggccgg aataacaggc gatgctgcac agatcgtcat
     5881 tggcgccggg attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca atcagggctg
     5941 gatcacactg caggaagcag ggattaacct ggacaggaat actctggccg ctatgctgat
     6001 ccgagagctg cgggcagccc tggaactgtt cgagcaggaa ggcctggctc catatctgtc
     6061 acggtgggag aagctggata acttcatcaa tagacccgtg aagctgatca ttggggacaa
     6121 agagattttc gggattagcc gggggattga taaacaggga gccctgctgc tggaacagga
     6181 cggagttatc aaaccctgga tgggcggaga aatcagtctg cggtctgccg aaaaggctag
     6241 cggcaagccc atccccaacc ccctgctggg cctggacagc accacaagtt tgtacaaaaa
     6301 agcaggctcc accatggcag cttatagaga accaccttgt aaccagtata caggtacaac
     6361 tacagctctt cagaaattgg aaggttttgc tagccggtta tttcatagac actctaaagg
     6421 tactgcacat gatcagaaaa cagctctgga aaatgacagc cttcatttct ctgagcatac
     6481 tgccttatgg gacagatcaa tgaaagagtt tctagccaaa gccaaagaag actttttgaa
     6541 aaaatgggag aatccaactc agaataatgc cggacttgaa gattttgaaa ggaaaaaaac
     6601 ccttggaaca ggttcatttg gaagagtcgt gttggtaaaa cacaaagcca ctgaacagta
     6661 ttatgccatg aagatcttag ataagcagaa ggttgttaaa ctgaagcaaa tagagcatac
     6721 tttgaatgag aaaagaatat tacaggcagt gaattttcct ttccttgttc gactggagta
     6781 tgcttttaag gataattcta atttatacat ggttatggaa tatgtccctg ggggtgagat
     6841 gttttcacat ctaagaagaa ttggaaggtt cagtgagccc catgcacggt tctatgcagc
     6901 tcagatagtg ctaacattcg agtacctcca ttcactagac ctcatctaca gagatctaaa
     6961 acctgaaaat ctcttaattg accatcaagg ctatatccag gtcacagact ttgggtttgc
     7021 caaaagagtt aaaggcagaa cttggacatt atgtggaact ccagagtatt tggctccaga
     7081 aataattctc agcaagggct acaataaggc agtggattgg tgggcattag gagtgctaat
     7141 ctatgaaatg gcagctggct atcccccatt ctttgcagac caaccaattc agatttatga
     7201 aaagattgtt tctggaaagg tccgattccc atcccacttc agttcagatc tcaaggacct
     7261 tctacggaac ctgctgcagg tggatttgac caagagattt ggaaatctaa agaatggtgt
     7321 cagtgatata aaaactcaca agtggtttgc cacgacagat tggattgcta tttaccagag
     7381 gaaggttgaa gctccattca taccaaagtt tagaggctct ggagatacca gcaactttga
     7441 tgactatgaa gaagaagata tccgtgtctc tataacagaa aaatgtgcaa aagaatttgg
     7501 tgaattttga atccacccag ctttcttgta caaagtggtg atatccagca cagtggcggc
     7561 cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7621 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7681 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7741 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7801 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7861 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7921 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     7981 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     8041 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     8101 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     8161 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     8221 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     8281 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     8341 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     8401 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     8461 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     8521 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     8581 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     8641 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt
     8701 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     8761 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     8821 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     8881 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     8941 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     9001 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     9061 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     9121 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     9181 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     9241 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
     9301 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatctcga gggcccggta
     9361 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     9421 gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact
     9481 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     9541 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     9601 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     9661 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     9721 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9781 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9841 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     9901 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     9961 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    10021 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
    10081 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
    10141 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
    10201 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
    10261 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
    10321 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
    10381 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
    10441 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
    10501 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    10561 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
    10621 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aatt
//
