LOCUS       sequence-335815-       10574 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway destination clone of STK32C (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10574)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-STK32C_IDG-K
FEATURES             Location/Qualifiers
     source          1..10574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     192..211
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             280..879
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(280..299)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     776..796
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1017..1250
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1322..1443
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1359..1378)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1413..1432
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1483..1618
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1638..1657)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1639..1657)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1667..1684)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1667..1683)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1676..1698)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1825..2280
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1912..1931)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2122..2143
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2306..2410
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2411..2479,2480..3271)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3442..4030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3931..3950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4184..4201
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4318..4339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4354..4359,4360..4377,4378..4384)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4392..4408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4397..4419
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4416..4432
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4416..4432
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4451..4471
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4453..4471
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4499..4725
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4726..4906
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4953..5078
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5571..5804
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5989..6033
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6300..6416
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6450..6470
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6470..7681
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7518..7537
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7685..7705)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      7706..7715
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7718..8488
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8495..8536
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8537..8561
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9678..9702)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9732..10320
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9785..9805)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10203..10214)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(10334..10574,1..259)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10369..10392)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
       61 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
      121 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
      181 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
      241 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
      301 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
      361 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
      421 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
      481 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
      541 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
      601 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
      661 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
      721 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
      781 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
      841 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
      901 cgcccgaccg aaaggagcgc acgaccccat gcatctcgag ggcccggtac ctttaagacc
      961 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     1021 agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     1081 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1141 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1201 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     1261 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     1321 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1381 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1441 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     1501 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     1561 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     1621 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     1681 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     1741 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     1801 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     1861 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     1921 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     1981 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     2041 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     2101 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     2161 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     2221 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     2281 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2341 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     2401 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     2461 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     2521 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2581 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2641 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2701 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2761 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2821 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2881 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2941 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3001 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3061 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3121 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3181 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3241 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3301 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3361 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3421 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3481 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3541 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3601 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3661 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3721 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3781 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3841 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3901 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3961 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4021 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4081 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4141 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4201 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     4261 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     4321 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     4381 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     4441 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     4501 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     4561 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     4621 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     4681 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     4741 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4801 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4861 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     4921 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     4981 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     5041 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     5101 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     5161 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     5221 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     5281 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     5341 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     5401 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     5461 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     5521 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     5581 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     5641 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     5701 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     5761 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     5821 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     5881 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     5941 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     6001 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     6061 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     6121 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     6181 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     6241 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     6301 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     6361 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     6421 tccagtgtgg tggaattctg cagatatcac aactttgtat agaaaagttg gcctccgcgc
     6481 cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac
     6541 gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc
     6601 tcataagact cggccttaga accccagtat cagcagaagg acattttagg acgggacttg
     6661 ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc
     6721 cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag
     6781 gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg
     6841 tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt
     6901 tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta
     6961 gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg
     7021 cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa
     7081 caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg
     7141 gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt
     7201 tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc
     7261 cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc
     7321 cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt
     7381 tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc
     7441 ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac
     7501 ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt
     7561 gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt
     7621 tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga
     7681 cggccaactt ttgtatacaa agtgggccac catgggaatc ccgctgctga acgctaaaca
     7741 gattctggga cagctggacg gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa
     7801 tcagtacctg ctggatcgaa tcggcgagct gaagagtggg gatgcttgca ttgcagaata
     7861 tcagcaggca gggagaggaa gcagagggag gaaatggttc tctccttttg gagctaacct
     7921 gtacctgagt atgttttggc gcctgaagcg gggaccagca gcaatcggcc tgggcccggt
     7981 catcggaatt gtcatggcag aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa
     8041 atggcccaat gacctgtatc tgcaggatag aaagctggca ggcatcctgg tggagctggc
     8101 cggaataaca ggcgatgctg cacagatcgt cattggcgcc gggattaacg tggctatgag
     8161 gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca ctgcaggaag cagggattaa
     8221 cctggacagg aatactctgg ccgctatgct gatccgagag ctgcgggcag ccctggaact
     8281 gttcgagcag gaaggcctgg ctccatatct gtcacggtgg gagaagctgg ataacttcat
     8341 caatagaccc gtgaagctga tcattgggga caaagagatt ttcgggatta gccgggggat
     8401 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
     8461 agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca accccctgct
     8521 gggcctggac agcaccacaa gtttgtacaa aaaagcaggc tccaccatgt acgccatgaa
     8581 gtacatgaac aagcagcagt gcatcgagcg cgacgaggtc cgcaacgtct tccgggagct
     8641 ggagatcctg caggagatcg agcacgtctt cctggtgaac ctctggtact ccttccagga
     8701 cgaggaggac atgttcatgg tcgtggacct gctactgggc ggggacctgc gctaccacct
     8761 gcagcagaac gtgcagttct ccgaggacac ggtgaggctg tacatctgcg agatggcact
     8821 ggctctggac tacctgcgcg gccagcacat catccacaga gatgtcaagc ctgacaacat
     8881 tctcctggat gagagaggac atgcacacct gaccgacttc aacattgcca ccatcatcaa
     8941 ggacggggag cgggcgacgg cattagcagg caccaagccg tacatggctc cggagatctt
     9001 ccactctttt gtcaacggcg ggaccggcta ctccttcgag gtggactggt ggtcggtggg
     9061 ggtgatggcc tatgagctgc tgcgaggatg gaggccctat gacatccact ccagcaacgc
     9121 cgtggagtcc ctggtgcagc tgttcagcac cgtgagcgtc cagtatgtcc ccacgtggtc
     9181 caaggagatg gtggccttgc tgcggaagct cctcgctgtg aaccccgagc accggctctc
     9241 cagcctccag gacgtgcagg cagccccggc gctggccggc gtgctgtggg accacctgag
     9301 cgagaagagg gtggagccgg gcttcgtgcc caacaaaggc cgtctgcact gcgaccccac
     9361 ctttgagctg gaggagatga tcctggagtc caggcccctg cacaagaaga agaagcgcct
     9421 ggccaagaac aagtcccggg acaacagcag ggacagctcc cagtccgaga atgactatct
     9481 tcaagactgc ctcgatgcca tccagcaaga cttcgtgatt tttaacagag aaaagctgaa
     9541 gaggagccag gacctcccga gggagcctct ccccgcccct gagtccaggg atgctgcgga
     9601 gcctgtggag gacgaggcgg aacgctccgc cctgcccatg tgcggcccca tttgcccctc
     9661 ggccgggagc ggctaggacc cagctttctt gtacaaagtg gtgatatcca gcacagtggc
     9721 ggccgctcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     9781 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     9841 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     9901 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     9961 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
    10021 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
    10081 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
    10141 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
    10201 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
    10261 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
    10321 ctggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta
    10381 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca
    10441 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact
    10501 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa
    10561 atggaagtag cacg
//
