LOCUS       sequence-336083-       10864 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Overexpression of RUNX1 S114L mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_S114L_Barcode
FEATURES             Location/Qualifiers
     source          1..10864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(528..1319,1320..1388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1151..1170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1389..1493)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1568..1587)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1759..2138
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2140..2338
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2293..2313
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2356..2536
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2583..2708
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3201..3434
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3619..3663
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3961..4078
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4214..5392
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4445..5383
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5340..5360
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     5409..5428
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5409..5427
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5491..6513
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5491..6513
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6523..6579
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(8132..8165)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8190..8370
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8396..8413)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8402..8626
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8672..9100
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8759..8778)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8969..8990
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9109..9129)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9114..9443
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9294..9429
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9491..9538
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9557..9931
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10061..10182
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10098..10117)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10152..10171
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10231..10247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10231..10247)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10244..10266)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10255..10271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10279..10285,10286..10303,10304..10309))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10324..10345
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10462..10479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10633..10864,1..357))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10713..10732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
       61 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      121 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      181 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      241 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      301 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      361 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
      421 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      481 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
      541 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
      601 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
      661 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
      721 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
      781 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
      841 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
      901 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
      961 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1021 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1081 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1141 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1201 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     1261 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     1321 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     1381 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1441 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     1501 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     1561 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     1621 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     1681 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     1741 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     1801 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     1861 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     1921 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     1981 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     2041 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     2101 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     2161 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     2221 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     2281 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
     2341 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2401 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2461 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2521 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     2581 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     2641 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     2701 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     2761 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     2821 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     2881 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     2941 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     3001 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     3061 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     3121 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     3181 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     3241 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     3301 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     3361 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     3421 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     3481 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     3541 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     3601 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     3661 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     3721 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     3781 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     3841 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     3901 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
     3961 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4021 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     4081 ggtttattac agggacagca gagatccagt ttggttaatt aatcgataag ctttgcaaag
     4141 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
     4201 aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     4261 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     4321 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     4381 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     4441 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     4501 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     4561 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     4621 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     4681 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     4741 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     4801 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     4861 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     4921 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     4981 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     5041 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     5101 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     5161 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     5221 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     5281 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     5341 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattag
     5401 cttggtacta atacgactca ctatagggag acccaagctg gctaggtaag cttgatatcg
     5461 aattcctgca gcccggggga tcccgccacc atgaaaaagc ctgaactcac cgcgacgtct
     5521 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     5581 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     5641 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     5701 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     5761 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     5821 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     5881 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     5941 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     6001 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     6061 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     6121 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     6181 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     6241 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     6301 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     6361 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     6421 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     6481 cgacgcccca gcactcgtcc gagggcaaag gaaggatccg gcgcaacaaa cttctctctg
     6541 ctgaaacaag ccggagatgt cgaagagaat cctggaccga tgcgtatccc cgtagatgcc
     6601 agcacgagcc gccgcttcac gccgccttcc accgcgctga gcccaggcaa gatgagcgag
     6661 gcgttgccgc tgggcgcccc ggacgccggc gctgccctgg ccggcaagct gaggagcggc
     6721 gaccgcagca tggtggaggt gctggccgac cacccgggcg agctggtgcg caccgacagc
     6781 cccaacttcc tctgctccgt gctgcctacg cactggcgct gcaacaagac cctgcccatc
     6841 gctttcaagg tggtggccct aggggatgtt ccagatggca ctctggtcac tgtgatggct
     6901 ggcaatgatg aaaactactt ggctgagctg agaaatgcta ccgcagccat gaagaaccag
     6961 gttgcaagat ttaatgacct caggtttgtc ggtcgaagtg gaagagggaa aagcttcact
     7021 ctgaccatca ctgtcttcac aaacccaccg caagtcgcca cctaccacag agccatcaaa
     7081 atcacagtgg atgggccccg agaacctcga agacatcggc agaaactaga tgatcagacc
     7141 aagcccggga gcttgtcctt ttccgagcgg ctcagtgaac tggagcagct gcggcgcaca
     7201 gccatgaggg tcagcccaca ccacccagcc cccacgccca accctcgtgc ctccctgaac
     7261 cactccactg cctttaaccc tcagcctcag agtcagatgc aggatacaag gcagatccaa
     7321 ccatccccac cgtggtccta cgatcagtcc taccaatacc tgggatccat tgcctctcct
     7381 tctgtgcacc cagcaacgcc catttcacct ggacgtgcca gcggcatgac aaccctctct
     7441 gcagaacttt ccagtcgact ctcaacggca cccgacctga cagcgttcag cgacccgcgc
     7501 cagttccccg cgctgccctc catctccgac ccccgcatgc actatccagg cgccttcacc
     7561 tactccccga cgccggtcac ctcgggcatc ggcatcggca tgtcggccat gggctcggcc
     7621 acgcgctacc acacctacct gccgccgccc taccccggct cgtcgcaagc gcagggaggc
     7681 ccgttccaag ccagctcgcc ctcctaccac ctgtactacg gcgcctcggc cggctcctac
     7741 cagttctcca tggtgggcgg cgagcgctcg ccgccgcgca tcctgccgcc ctgcaccaac
     7801 gcctccaccg gctccgcgct gctcaacccc agcctcccga accagagcga cgtggtggag
     7861 gccgagggca gccacagcaa ctcccccacc aacatggcgc cctccgcgcg cctggaggag
     7921 gccgtgtgga ggccctactg actagctaag ttagaactat ttcctggctg ttacgcgccc
     7981 tagtatgggc tactgacggg cttgttaaac agtgaaccgc ccgccccacg acccgcagcg
     8041 cccgaccgaa aggagcgcac gaccccatgc atcgaacaaa agaaaagggg gactggaagg
     8101 gctaattcac tcccaacgaa gacaagatat cataacttcg tatagcatac attatacgaa
     8161 gttatcgtct agctggtccg gactgtactg ggtctctctg gttagaccag atctgagcct
     8221 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8281 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8341 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
     8401 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     8461 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     8521 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     8581 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     8641 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     8701 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8761 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8821 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8881 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8941 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9001 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     9061 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     9121 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     9181 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9241 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     9301 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     9361 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     9421 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     9481 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     9541 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     9601 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     9661 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     9721 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     9781 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     9841 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     9901 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     9961 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
    10021 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
    10081 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    10141 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
    10201 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    10261 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    10321 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    10381 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    10441 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    10501 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10561 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10621 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10681 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10741 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10801 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10861 ggtc
//
