LOCUS       sequence-336087-       10865 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Overexpression of RUNX1 M106R mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_M106R_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(103..123)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        108..437
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      288..423
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        485..532
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             551..925
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1055..1176
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1092..1111)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1146..1165
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1225..1241)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1225..1241)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1238..1260)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1249..1265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1273..1279,1280..1297,1298..1303))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1318..1339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1456..1473)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1627..2215)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1707..1726)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2386..3177,3178..3246))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3009..3028
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3247..3351)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3426..3445)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3617..3996
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3998..4196
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4151..4171
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4214..4394
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4441..4566
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5059..5292
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5477..5521
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5819..5936
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6072..7250
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6303..7241
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7198..7218
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7267..7286
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7267..7285
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7349..8371
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7349..8371
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8381..8437
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9991..10024)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10049..10229
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10255..10272)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10261..10485
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10531..10865,1..94)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10618..10637)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10828..10849
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
       61 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
      121 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
      181 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      241 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
      301 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
      361 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
      421 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
      481 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
      541 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
      601 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
      661 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
      721 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
      781 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
      841 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
      901 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
      961 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     1021 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     1081 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     1141 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     1201 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     1261 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     1321 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     1381 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1441 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1501 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1561 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1621 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1681 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1741 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1801 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1861 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1921 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1981 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2041 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2101 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2161 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2221 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2281 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2341 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2401 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2461 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2521 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2581 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     2641 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     2701 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2761 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2821 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2881 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2941 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3001 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3061 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3121 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3181 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3241 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3301 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3361 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     3421 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     3481 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     3541 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     3601 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     3661 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     3721 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     3781 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     3841 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     3901 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     3961 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     4021 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     4081 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     4141 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     4201 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4261 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4321 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4381 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     4441 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     4501 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     4561 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     4621 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     4681 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     4741 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     4801 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     4861 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     4921 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     4981 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     5041 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     5101 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     5161 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     5221 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     5281 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     5341 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     5401 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     5461 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     5521 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     5581 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     5641 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     5701 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     5761 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     5821 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     5881 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     5941 tttattacag ggacagcaga gatccagttt ggttaattaa tcgataagct ttgcaaagat
     6001 ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag
     6061 gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     6121 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     6181 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     6241 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     6301 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
     6361 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
     6421 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
     6481 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
     6541 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
     6601 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
     6661 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
     6721 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
     6781 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     6841 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     6901 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     6961 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     7021 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     7081 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     7141 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     7201 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaattagct
     7261 tggtactaat acgactcact atagggagac ccaagctggc taggtaagct tgatatcgaa
     7321 ttcctgcagc ccgggggatc ccgccaccat gaaaaagcct gaactcaccg cgacgtctgt
     7381 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     7441 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     7501 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     7561 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
     7621 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     7681 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     7741 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     7801 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     7861 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     7921 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     7981 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     8041 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     8101 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     8161 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     8221 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     8281 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     8341 acgccccagc actcgtccga gggcaaagga aggatccggc gcaacaaact tctctctgct
     8401 gaaacaagcc ggagatgtcg aagagaatcc tggaccgctg cgtatccccg tagatgccag
     8461 cacgagccgc cgcttcacgc cgccttccac cgcgctgagc ccaggcaaga tgagcgaggc
     8521 gttgccgctg ggcgccccgg acgccggcgc tgccctggcc ggcaagctga ggagcggcga
     8581 ccgcagcatg gtggaggtgc tggccgacca cccgggcgag ctggtgcgca ccgacagccc
     8641 caacttcctc tgctccgtgc tgcctacgca ctggcgctgc aacaagaccc tgcccatcgc
     8701 tttcaaggtg gtggccctag gggatgttcc agatggcact ctggtcactg tgagggctgg
     8761 caatgatgaa aactactcgg ctgagctgag aaatgctacc gcagccatga agaaccaggt
     8821 tgcaagattt aatgacctca ggtttgtcgg tcgaagtgga agagggaaaa gcttcactct
     8881 gaccatcact gtcttcacaa acccaccgca agtcgccacc taccacagag ccatcaaaat
     8941 cacagtggat gggccccgag aacctcgaag acatcggcag aaactagatg atcagaccaa
     9001 gcccgggagc ttgtcctttt ccgagcggct cagtgaactg gagcagctgc ggcgcacagc
     9061 catgagggtc agcccacacc acccagcccc cacgcccaac cctcgtgcct ccctgaacca
     9121 ctccactgcc tttaaccctc agcctcagag tcagatgcag gatacaaggc agatccaacc
     9181 atccccaccg tggtcctacg atcagtccta ccaatacctg ggatccattg cctctccttc
     9241 tgtgcaccca gcaacgccca tttcacctgg acgtgccagc ggcatgacaa ccctctctgc
     9301 agaactttcc agtcgactct caacggcacc cgacctgaca gcgttcagcg acccgcgcca
     9361 gttccccgcg ctgccctcca tctccgaccc ccgcatgcac tatccaggcg ccttcaccta
     9421 ctccccgacg ccggtcacct cgggcatcgg catcggcatg tcggccatgg gctcggccac
     9481 gcgctaccac acctacctgc cgccgcccta ccccggctcg tcgcaagcgc agggaggccc
     9541 gttccaagcc agctcgccct cctaccacct gtactacggc gcctcggccg gctcctacca
     9601 gttctccatg gtgggcggcg agcgctcgcc gccgcgcatc ctgccgccct gcaccaacgc
     9661 ctccaccggc tccgcgctgc tcaaccccag cctcccgaac cagagcgacg tggtggaggc
     9721 cgagggcagc cacagcaact cccccaccaa catggcgccc tccgcgcgcc tggaggaggc
     9781 cgtgtggagg ccctactgac tagctaagtt agaactattt cctggctgtt acgcgacttc
     9841 acattatcta ctgacgggct tgttaaacag tgaaccgccc gccccacgac ccgcagcgcc
     9901 cgaccgaaag gagcgcacga ccccatgcat cgaacaaaag aaaagggggg actggaaggg
     9961 ctaattcact cccaacgaag acaagatatc ataacttcgt atagcataca ttatacgaag
    10021 ttatcgtcta gctggtccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
    10081 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    10141 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    10201 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
    10261 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    10321 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    10381 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    10441 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
    10501 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
    10561 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
    10621 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
    10681 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
    10741 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
    10801 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
    10861 accct
//
