LOCUS       sequence-336089-       10865 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Overexpression of RUNX1 L102P mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_L102P_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(51..71)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        56..385
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      236..371
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        433..480
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             499..873
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1003..1124
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1040..1059)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1094..1113
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1173..1189)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1173..1189)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1186..1208)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1197..1213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1221..1227,1228..1245,1246..1251))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1266..1287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1404..1421)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1575..2163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1655..1674)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2334..3125,3126..3194))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2957..2976
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3195..3299)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3374..3393)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3565..3944
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3946..4144
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4099..4119
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4162..4342
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4389..4514
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5007..5240
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5425..5469
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5767..5884
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6020..7198
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6251..7189
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7146..7166
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7215..7234
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7215..7233
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7297..8319
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7297..8319
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8329..8385
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9939..9972)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9997..10177
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10203..10220)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10209..10433
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10479..10865,1..42)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10566..10585)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10776..10797
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
       61 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      121 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      181 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
      241 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
      301 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
      361 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
      421 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
      481 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
      541 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
      601 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
      661 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
      721 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
      781 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
      841 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
      901 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
      961 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     1021 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     1081 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     1141 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     1201 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     1261 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     1321 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1381 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1441 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1501 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1561 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1621 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1681 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1741 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1801 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1861 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1921 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1981 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     2041 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2101 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2161 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2221 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2281 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2341 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2401 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     2461 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     2521 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     2581 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     2641 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     2701 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     2761 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2821 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2881 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2941 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3001 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3061 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3121 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3181 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3241 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3301 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     3361 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     3421 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     3481 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     3541 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     3601 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     3661 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     3721 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     3781 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     3841 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     3901 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     3961 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     4021 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     4081 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     4141 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4201 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4261 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4321 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     4381 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     4441 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     4501 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     4561 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     4621 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     4681 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     4741 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     4801 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     4861 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     4921 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     4981 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     5041 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     5101 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     5161 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     5221 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     5281 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     5341 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     5401 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     5461 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     5521 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     5581 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     5641 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     5701 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
     5761 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     5821 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     5881 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaatc gataagcttt
     5941 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     6001 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     6061 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     6121 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     6181 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     6241 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     6301 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     6361 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     6421 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     6481 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     6541 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     6601 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     6661 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     6721 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     6781 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     6841 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     6901 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     6961 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     7021 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     7081 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     7141 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg
     7201 aattagcttg gtactaatac gactcactat agggagaccc aagctggcta ggtaagcttg
     7261 atatcgaatt cctgcagccc gggggatccc gccaccatga aaaagcctga actcaccgcg
     7321 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     7381 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     7441 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     7501 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     7561 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     7621 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     7681 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     7741 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     7801 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     7861 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     7921 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     7981 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     8041 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     8101 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     8161 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     8221 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     8281 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaag gatccggcgc aacaaacttc
     8341 tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatgcg tatccccgta
     8401 gatgccagca cgagccgccg cttcacgccg ccttccaccg cgctgagccc aggcaagatg
     8461 agcgaggcgt tgccgctggg cgccccggac gccggcgctg ccctggccgg caagctgagg
     8521 agcggcgacc gcagcatggt ggaggtgctg gccgaccacc cgggcgagct ggtgcgcacc
     8581 gacagcccca acttcctctg ctccgtgctg cctacgcact ggcgctgcaa caagaccctg
     8641 cccatcgctt tcaaggtggt ggccctaggg gatgttccag atggcactcc ggtcactgtg
     8701 atggctggca atgatgaaaa ctactcggct gagctgagaa atgctaccgc agccatgaag
     8761 aaccaggttg caagatttaa tgacctcagg tttgtcggtc gaagtggaag agggaaaagc
     8821 ttcactctga ccatcactgt cttcacaaac ccaccgcaag tcgccaccta ccacagagcc
     8881 atcaaaatca cagtggatgg gccccgagaa cctcgaagac atcggcagaa actagatgat
     8941 cagaccaagc ccgggagctt gtccttttcc gagcggctca gtgaactgga gcagctgcgg
     9001 cgcacagcca tgagggtcag cccacaccac ccagccccca cgcccaaccc tcgtgcctcc
     9061 ctgaaccact ccactgcctt taaccctcag cctcagagtc agatgcagga tacaaggcag
     9121 atccaaccat ccccaccgtg gtcctacgat cagtcctacc aatacctggg atccattgcc
     9181 tctccttctg tgcacccagc aacgcccatt tcacctggac gtgccagcgg catgacaacc
     9241 ctctctgcag aactttccag tcgactctca acggcacccg acctgacagc gttcagcgac
     9301 ccgcgccagt tccccgcgct gccctccatc tccgaccccc gcatgcacta tccaggcgcc
     9361 ttcacctact ccccgacgcc ggtcacctcg ggcatcggca tcggcatgtc ggccatgggc
     9421 tcggccacgc gctaccacac ctacctgccg ccgccctacc ccggctcgtc gcaagcgcag
     9481 ggaggcccgt tccaagccag ctcgccctcc taccacctgt actacggcgc ctcggccggc
     9541 tcctaccagt tctccatggt gggcggcgag cgctcgccgc cgcgcatcct gccgccctgc
     9601 accaacgcct ccaccggctc cgcgctgctc aaccccagcc tcccgaacca gagcgacgtg
     9661 gtggaggccg agggcagcca cagcaactcc cccaccaaca tggcgccctc cgcgcgcctg
     9721 gaggaggccg tgtggaggcc ctactgacta gctaagttag aactatttcc tggctgttac
     9781 gcgtgtcccg tacacctact gacgggcttg ttaaacagtg aaccgcccgc cccacgaccc
     9841 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg aacaaaagaa aaggggggac
     9901 tggaagggct aattcactcc caacgaagac aagatatcat aacttcgtat agcatacatt
     9961 atacgaagtt atcgtctagc tggtccggac tgtactgggt ctctctggtt agaccagatc
    10021 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    10081 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    10141 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
    10201 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
    10261 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
    10321 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
    10381 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
    10441 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
    10501 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
    10561 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
    10621 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
    10681 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
    10741 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
    10801 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
    10861 ctatt
//
