LOCUS       sequence-336435-       10865 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Overexpression of RUNX1 L71P mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_L71P_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(75..95)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        80..409
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      260..395
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        457..504
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             523..897
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1027..1148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1064..1083)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1118..1137
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1197..1213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1197..1213)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1210..1232)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1221..1237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1245..1251,1252..1269,1270..1275))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1290..1311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1428..1445)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1599..2187)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1679..1698)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2358..3149,3150..3218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2981..3000
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3219..3323)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3398..3417)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3589..3968
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3970..4168
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4123..4143
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4186..4366
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4413..4538
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5031..5264
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5449..5493
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5791..5908
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6044..7222
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6275..7213
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7170..7190
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7239..7258
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7239..7257
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7321..8343
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7321..8343
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8353..8409
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9963..9996)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10021..10201
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10227..10244)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10233..10457
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10503..10865,1..66)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10590..10609)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10800..10821
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
       61 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
      121 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
      181 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
      241 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      301 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
      361 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
      421 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
      481 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
      541 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
      601 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
      661 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
      721 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
      781 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
      841 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
      901 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
      961 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     1021 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1141 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     1201 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1261 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1321 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1381 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1441 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1501 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1561 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1621 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1681 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1741 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1801 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1861 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1921 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1981 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2041 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2101 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2161 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2221 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2281 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2581 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2641 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2701 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2761 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2821 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2881 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2941 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3001 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3061 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3241 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3301 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     3361 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     3421 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     3481 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     3541 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     3601 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     3661 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3721 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3781 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3841 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     3901 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     3961 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     4021 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4081 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4141 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
     4201 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4261 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4321 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     4381 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     4441 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     4501 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     4561 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     4621 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     4681 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     4741 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     4801 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     4861 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     4921 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     4981 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     5041 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     5101 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     5161 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     5221 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     5281 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     5341 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     5401 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     5461 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     5521 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     5581 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     5641 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     5701 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     5761 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     5821 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     5881 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     5941 ttggttaatt aatcgataag ctttgcaaag atggataaag ttttaaacag agaggaatct
     6001 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     6061 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     6121 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     6181 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     6241 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     6301 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     6361 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     6421 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     6481 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     6541 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     6601 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     6661 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     6721 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     6781 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     6841 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     6901 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     6961 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     7021 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     7081 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     7141 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     7201 tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag
     7261 acccaagctg gctaggtaag cttgatatcg aattcctgca gcccggggga tcccgccacc
     7321 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     7381 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     7441 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     7501 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     7561 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     7621 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     7681 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     7741 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     7801 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     7861 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     7921 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     7981 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     8041 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     8101 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     8161 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     8221 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     8281 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     8341 gaaggatccg gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt cgaagagaat
     8401 cctggaccga tgcgtatccc cgtagatgcc agcacgagcc gccgcttcac gccgccttcc
     8461 accgcgctga gcccaggcaa gatgagcgag gcgttgccgc tgggcgcccc ggacgccggc
     8521 gctgccctgg ccggcaagct gaggagcggc gaccgcagca tggtggaggt gctggccgac
     8581 cacccgggcg agctggtgcg caccgacagc cccaacttcc cctgctccgt gctgcctacg
     8641 cactggcgct gcaacaagac cctgcccatc gctttcaagg tggtggccct aggggatgtt
     8701 ccagatggca ctctggtcac tgtgatggct ggcaatgatg aaaactactc ggctgagctg
     8761 agaaatgcta ccgcagccat gaagaaccag gttgcaagat ttaatgacct caggtttgtc
     8821 ggtcgaagtg gaagagggaa aagcttcact ctgaccatca ctgtcttcac aaacccaccg
     8881 caagtcgcca cctaccacag agccatcaaa atcacagtgg atgggccccg agaacctcga
     8941 agacatcggc agaaactaga tgatcagacc aagcccggga gcttgtcctt ttccgagcgg
     9001 ctcagtgaac tggagcagct gcggcgcaca gccatgaggg tcagcccaca ccacccagcc
     9061 cccacgccca accctcgtgc ctccctgaac cactccactg cctttaaccc tcagcctcag
     9121 agtcagatgc aggatacaag gcagatccaa ccatccccac cgtggtccta cgatcagtcc
     9181 taccaatacc tgggatccat tgcctctcct tctgtgcacc cagcaacgcc catttcacct
     9241 ggacgtgcca gcggcatgac aaccctctct gcagaacttt ccagtcgact ctcaacggca
     9301 cccgacctga cagcgttcag cgacccgcgc cagttccccg cgctgccctc catctccgac
     9361 ccccgcatgc actatccagg cgccttcacc tactccccga cgccggtcac ctcgggcatc
     9421 ggcatcggca tgtcggccat gggctcggcc acgcgctacc acacctacct gccgccgccc
     9481 taccccggct cgtcgcaagc gcagggaggc ccgttccaag ccagctcgcc ctcctaccac
     9541 ctgtactacg gcgcctcggc cggctcctac cagttctcca tggtgggcgg cgagcgctcg
     9601 ccgccgcgca tcctgccgcc ctgcaccaac gcctccaccg gctccgcgct gctcaacccc
     9661 agcctcccga accagagcga cgtggtggag gccgagggca gccacagcaa ctcccccacc
     9721 aacatggcgc cctccgcgcg cctggaggag gccgtgtgga ggccctactg actagctaag
     9781 ttagaactat ttcctggctg ttacgcgcat cgctgatgtc tactgacggg cttgttaaac
     9841 agtgaaccgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
     9901 atcgaacaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     9961 tcataacttc gtatagcata cattatacga agttatcgtc tagctggtcc ggactgtact
    10021 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
    10081 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
    10141 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
    10201 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
    10261 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
    10321 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
    10381 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
    10441 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
    10501 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    10561 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    10621 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    10681 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    10741 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    10801 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    10861 ataag
//
