LOCUS       sequence-33679-e         605 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-iV
FEATURES             Location/Qualifiers
     source          1..605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(17..25,26..28,29..603)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(190..209)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     419..436
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 cgcccttgct caccatggtt gtggccatat tatcatcgtg tttttcaaag gaaaaccacg
       61 tccccgtggt tcggggggcc tagacgtttt tttaacctcg actaaacaca tgtaaagcat
      121 gtgcaccgag gccccagatc agatcccata caatggggta ccttctgggc atccttcagc
      181 cccttgttga atacgcttga ggagagccat ttgactcttt ccacaactat ccaactcaca
      241 acgtggcact ggggttgtgc cgcctttgca ggtgtatctt atacacgtgg cttttggccg
      301 cagaggcacc tgtcgccagg tggggggttc cgctgcctgc aaagggtcgc tacagacgtt
      361 gtttgtcttc aagaagcttc cagaggaact gcttccttca cgacattcaa cagaccttgc
      421 attcctttgg cgagagggga aagaccccta ggaatgctcg tcaagaagac agggccaggt
      481 ttccgggccc tcacattgcc aaaagacggc aatatggtgg aaaataacat atagacaaac
      541 gcacaccggc cttattccaa gcggcttcgg ccagtaacgt tagggggggg ggagggagag
      601 ggggg
//
