LOCUS       sequence-336898-       11189 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Drosophila transgene expression under QUAS/QF control, PhiC31 
            transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11189)
  TITLE     McCabe lab pBID2 plasmids 2021
REFERENCE   2  (bases 1 to 11189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBID2_QUAS>Gateway
FEATURES             Location/Qualifiers
     source          1..11189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4792..4825
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4838..5268
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5712..5836
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5861..5866,5867..5884,5885..5891)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5945..6604
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6011..6030)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6946..7251
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7130..7149
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7292..7416)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    8113..8247
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8150..8169)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8204..8223
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    8249..8679
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    8713..8782
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(9001..9019)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9040..9056)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9040..9056)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9053..9075)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9064..9080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9088..9094,9095..9112,9113..9118))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9133..9154
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9271..9288)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9442..10030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9522..9541)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10201..10992,10993..11061))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10824..10843
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11062..11166)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 tccaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag cataacttcg
     4801 tataatgtat gctatacgaa gttatgctag cggatcctgg ccacgtaata agtgtgcgtt
     4861 gaatttattc gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta
     4921 tcaaaaaata agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg
     4981 tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa
     5041 acaaattttc gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa
     5101 atacattgca taccctcttt taataaaaaa tattgcatac gttgacgaaa caaattttcg
     5161 ttgcataccc aataaaagat tattatattg catacctttt cttgccatac catttagccg
     5221 atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa ccaacaacag atccaagctg
     5281 gccgcggctc gataagcttg tcactgggtc agtgggtaat cgcttatcct cggataaaca
     5341 attatcctca cgggtaatcg cttatccgct cgggtaatcg cttatcctcg ggtaatcgct
     5401 tatccttggg taatcgctta tcctcggata aacaattatc ctcacgggta atcgcttatc
     5461 cgctcgggta atcgcttatc ctcgggtaat cgcttatcct ttcacgttgg gacgtcgaga
     5521 ctctagcgag ctcgcccggg gatcgagcgc agcggtataa aagggcgcgg ggtggctgag
     5581 agcatcagtt gtgaatgaat gttcgagccg agcagacgtg ccgctgcctt cgttaatatc
     5641 ctttgaataa gccaactttg aatcacaaga cgcataccaa acgaattcgc agatcttaat
     5701 taaccggtac cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata
     5761 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
     5821 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
     5881 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
     5941 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     6001 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     6061 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     6121 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     6181 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     6241 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     6301 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     6361 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     6421 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     6481 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     6541 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     6601 gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
     6661 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat
     6721 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca
     6781 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
     6841 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
     6901 ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt
     6961 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
     7021 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
     7081 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     7141 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
     7201 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
     7261 tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca
     7321 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
     7381 ttacgtttct cgttcagctt tcttgtacaa agtggtgata tcgcatgcgg ccgcggcgcg
     7441 cctctagaaa tgaatcgttt ttaaaataac aaatcaattg ttttataata ttcgtacgat
     7501 tctttgatta tgtaataaaa tgtgatcatt aggaagatta cgaaaaatat aaaaaatatg
     7561 agttctgtgt gtataacaaa tgctgtaaac gccacaattg tgtttgttgc aaataaaccc
     7621 atgattattt gattaaaatt gttgttttct ttgttcatag acaatagtgt gttttgccta
     7681 aacgtgtact gcataaactc catgcgagtg tatagcgagc tagtggctaa cgcttgcccc
     7741 accaaagtag attcgtcaaa atcctcaatt tcatcaccct cctccaagtt taacatttgg
     7801 ccgtcggaat taacttctaa agatgccaca taatctaata aatgaaatag agattcaaac
     7861 gtggcgtcat cgtccgtttc gaccatttcc gaaaagaact cgggcataaa ctctatgatt
     7921 tctctggacg tggtgttgtc gaaactctca aagtacgcag tcaggaacgt gcgcgacatg
     7981 tcgtcgggaa actcgcgcgg aaacatgttg ttgtaaccga acgggtccca tagcgccaaa
     8041 accaaatctg ccagcgtcaa tagaatgagc acgatgccga caatggagct ggcttggata
     8101 gcgattcgag ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8161 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8221 atcaatgtat cttatcatgt ctggatcttg gccacgtaat aagtgtgcgt tgaatttatt
     8281 cgcaaaaaca ttgcatattt tcggcaaagt aaaattttgt tgcatacctt atcaaaaaat
     8341 aagtgctgca tactttttag agaaaccaaa taatttttta ttgcataccc gtttttaata
     8401 aaatacattg cataccctct tttaataaaa aatattgcat actttgacga aacaaatttt
     8461 cgttgcatac ccaataaaag attattatat tgcatacccg tttttaataa aatacattgc
     8521 ataccctctt ttaataaaaa atattgcata cgttgacgaa acaaattttc gttgcatacc
     8581 caataaaaga ttattatatt gcataccttt tcttgccata ccatttagcc gatcaattgt
     8641 gctcggcaac agtatatttg tggtgtgcca accaacaacg gatccactag tgtcgacgat
     8701 gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg
     8761 cgcgtactcc acctcaccca tctggtccat catgatgaac gggtcgaggt ggcggtagtt
     8821 gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt
     8881 ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt
     8941 cagcgggttc tcgacggtca cggcgggcat gtcgacacta gttctagcca gcttttgttc
     9001 cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     9061 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     9121 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9181 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9241 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9301 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9361 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9421 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9481 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9541 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9601 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9661 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9721 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9781 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9841 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9901 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9961 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10021 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10081 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10141 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10201 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10261 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10321 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10381 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10441 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10501 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10561 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10621 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10681 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10741 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    10801 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    10861 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    10921 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10981 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11041 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11101 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11161 tccgcgcaca tttccccgaa aagtgccac
//
