LOCUS       sequence-337149-        9200 bp    DNA     circular SYN 14-APR-2025
DEFINITION  expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for 
            pseudovirus production..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9200)
  AUTHORS   Cho H, Gonzales-Wartz KK, Huang D, Yuan M, Peterson M, Liang J, 
            Beutler N, Torres JL, Cong Y, Postnikova E, Bangaru S, Talana CA, 
            Shi W, Yang ES, Zhang Y, Leung K, Wang L, Peng L, Skinner J, Li S, 
            Wu NC, Liu H, Dacon C, Moyer T, Cohen M, Zhao M, Lee FE, Weinberg 
            RS, Douagi I, Gross R, Schmaljohn C, Pegu A, Mascola JR, Holbrook M,
            Nemazee D, Rogers TF, Ward AB, Wilson IA, Crompton PD, Tan J
  TITLE     Bispecific antibodies targeting distinct regions of the spike 
            protein potently neutralize SARS-CoV-2 variants of concern.
  JOURNAL   Sci Transl Med. 2021 Oct 20;13(616):eabj5413. doi: 
            10.1126/scitranslmed.abj5413. Epub 2021 Oct 20.
  PUBMED    34519517
REFERENCE   2  (bases 1 to 9200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2021-10-20"; volume: "13(616):eabj5413. doi"; pages: "
            10.1126/scitranslmed.abj5413. Epub 2021 Oct 20"
COMMENT     Sequence Label: pcDNA3.3-SARS2-B.1.617.2
FEATURES             Location/Qualifiers
     source          1..9200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     542..562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     588..612
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             complement(2024..2041)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     primer_bind     complement(4544..4563)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4588..4636
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4838..5266
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4925..4944)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5135..5156
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5275..5295)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5280..5609
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5460..5595
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5522..5541
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5676..6470
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5730..5749)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6340..6359
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6646..6767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6683..6702)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6737..6756
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6816..6832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6816..6832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6829..6851)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6840..6856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6864..6870,6871..6888,6889..6894))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6909..6930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7047..7064)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7218..7806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7298..7317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7977..8768,8769..8837))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8600..8619
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8838..8942)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9017..9036)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      601 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
      661 gaccgatcca gcctccggac tgagctcctt aaggttaact ctagagaatt cggatccatg
      721 tttgtgttcc tggtgctgct gccactggtg tccagccagt gtgtgaacct gaggaccagg
      781 acccaacttc ctcctgccta caccaactcc ttcaccaggg gagtctacta ccctgacaag
      841 gtgttcaggt cctctgtgct gcacagcacc caggacctgt tcctgccatt cttcagcaat
      901 gtgacctggt tccatgccat ccatgtgtct ggcaccaatg gcaccaagag gtttgacaac
      961 cctgtgctgc cattcaatga tggagtctac tttgccagca cagagaagag caacatcatc
     1021 aggggctgga tttttggcac caccctggac agcaagaccc agtccctgct gattgtgaac
     1081 aatgccacca atgtggtgat taaggtgtgt gagttccagt tctgtaatga cccattcctg
     1141 ggagtctact accacaagaa caacaagtcc tggatggagt ctggggtcta ctcctctgcc
     1201 aacaactgta cctttgaata tgtgagccaa ccattcctga tggacttgga gggcaagcag
     1261 ggcaacttca agaacctgag ggagtttgtg ttcaagaaca ttgatggcta cttcaagatt
     1321 tacagcaaac acacaccaat caacctggtg agggacctgc cacagggctt ctctgccttg
     1381 gaaccactgg tggacctgcc aattggcatc aacatcacca ggttccagac cctgctggct
     1441 ctgcacaggt cctacctgac acctggagac tcctcctctg gctggacagc aggagcagca
     1501 gcctactatg tgggctacct ccaaccaagg accttcctgc tgaaatacaa tgagaatggc
     1561 accatcacag atgctgtgga ctgtgccctg gacccactgt ctgagaccaa gtgtaccctg
     1621 aaatccttca cagtggagaa gggcatctac cagaccagca acttcagggt ccaaccaaca
     1681 gagagcattg tgaggtttcc aaacatcacc aacctgtgtc catttggaga ggtgttcaat
     1741 gccaccaggt ttgcctctgt ctatgcctgg aacaggaaga ggattagcaa ctgtgtggct
     1801 gactactctg tgctctacaa ctctgcctcc ttcagcacct tcaagtgtta tggagtgagc
     1861 ccaaccaaac tgaatgacct gtgtttcacc aatgtctatg ctgactcctt tgtgattagg
     1921 ggagatgagg tgagacagat tgcccctgga caaacaggca agattgctga ctacaactac
     1981 aaactgcctg atgacttcac aggctgtgtg attgcctgga acagcaacaa cctggacagc
     2041 aaggtgggag gcaactacaa ctacaggtac agactgttca ggaagagcaa cctgaaacca
     2101 tttgagaggg acatcagcac agagatttac caggctggca gcaaaccatg taatggagtg
     2161 gagggcttca actgttactt tccactccaa tcctatggct tccaaccaac caatggagtg
     2221 ggctaccaac catacagggt ggtggtgctg tcctttgaac tgctccatgc ccctgccaca
     2281 gtgtgtggac caaagaagag caccaacctg gtgaagaaca agtgtgtgaa cttcaacttc
     2341 aatggactga caggcacagg agtgctgaca gagagcaaca agaagttcct gccattccaa
     2401 cagtttggca gggacattgc tgacaccaca gatgctgtga gggacccaca gaccttggag
     2461 attctggaca tcacaccatg ttcctttgga ggagtgtctg tgattacacc tggcaccaac
     2521 accagcaacc aggtggctgt gctctaccag ggtgtgaact gtactgaggt gcctgtggct
     2581 atccatgctg accaacttac accaacctgg agggtctaca gcacaggcag caatgtgttc
     2641 cagaccaggg ctggctgtct gattggagca gagcatgtga acaactccta tgagtgtgac
     2701 atcccaattg gagcaggcat ctgtgcctcc taccagaccc agaccaacag cagaaggagg
     2761 gcaaggtctg tggcaagcca gagcatcatt gcctacacaa tgagtctggg agcagagaac
     2821 tctgtggctt acagcaacaa cagcattgcc atcccaacca acttcaccat ctctgtgacc
     2881 acagagattc tgcctgtgag tatgaccaag acctctgtgg actgtacaat gtatatctgt
     2941 ggagacagca cagagtgtag caacctgctg ctccaatatg gctccttctg tacccaactt
     3001 aacagggctc tgacaggcat tgctgtggaa caggacaaga acacccagga ggtgtttgcc
     3061 caggtgaagc agatttacaa gacacctcca atcaaggact ttggaggctt caacttcagc
     3121 cagattctgc ctgacccaag caagccaagc aagaggtcct tcattgagga cctgctgttc
     3181 aacaaggtga ccctggctga tgctggcttc atcaagcaat atggagactg tctgggagac
     3241 attgctgcca gggacctgat ttgtgcccag aagttcaatg gactgacagt gctgcctcca
     3301 ctgctgacag atgagatgat tgcccaatac acctctgccc tgctggctgg caccatcacc
     3361 tctggctgga cctttggagc aggagcagcc ctccaaatcc catttgctat gcagatggct
     3421 tacaggttca atggcattgg agtgacccag aatgtgctct atgagaacca gaaactgatt
     3481 gccaaccagt tcaactctgc cattggcaag attcaggact ccctgtccag cacagcctct
     3541 gccctgggca aactccaaaa tgtggtgaac cagaatgccc aggctctgaa caccctggtg
     3601 aagcaacttt ccagcaactt tggagccatc tcctctgtgc tgaatgacat cctgagcaga
     3661 ctggacaagg tggaggctga ggtccagatt gacagactga ttacaggcag actccaatcc
     3721 ctccaaacct atgtgaccca acaacttatc agggctgctg agattagggc atctgccaac
     3781 ctggctgcca ccaagatgag tgagtgtgtg ctgggacaaa gcaagagggt ggacttctgt
     3841 ggcaagggct accacctgat gagttttcca cagtctgccc ctcatggagt ggtgttcctg
     3901 catgtgacct atgtgcctgc ccaggagaag aacttcacca cagcccctgc catctgccat
     3961 gatggcaagg ctcactttcc aagggaggga gtgtttgtga gcaatggcac ccactggttt
     4021 gtgacccaga ggaacttcta tgaaccacag attatcacca cagacaacac ctttgtgtct
     4081 ggcaactgtg atgtggtgat tggcattgtg aacaacacag tctatgaccc actccaacct
     4141 gaactggact ccttcaagga ggaactggac aaatacttca agaaccacac cagccctgat
     4201 gtggacctgg gagacatctc tggcatcaat gcctctgtgg tgaacatcca gaaggagatt
     4261 gacagactga atgaggtggc taagaacctg aatgagtccc tgattgacct ccaagaactg
     4321 ggcaaatatg aacaatacat caagtggcca tggtacatct ggctgggctt cattgctgga
     4381 ctgattgcca ttgtgatggt gaccataatg ctgtgttgta tgacctcctg ttgttcctgt
     4441 ctgaaaggct gttgttcctg tggctcctgt tgtaagtgac tcgagccatg gaagcttgat
     4501 atctaactga ctgaaccggt ggtaccaaac gggggaggct aactgaaaca cggaaggaga
     4561 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt
     4621 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     4681 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     4741 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     4801 gcagatctgc gcagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     4861 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4921 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4981 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5041 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5101 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5161 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5221 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     5281 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5341 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5401 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     5461 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5521 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     5581 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     5641 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     5701 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5761 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     5821 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     5881 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     5941 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     6001 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     6061 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     6121 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     6181 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     6241 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     6301 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     6361 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     6421 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     6481 ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     6541 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6601 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     6661 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     6781 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6841 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6901 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6961 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7021 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7081 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7141 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7201 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7261 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7321 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7381 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7441 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7501 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7561 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7621 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7681 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7741 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7801 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7861 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7921 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7981 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8041 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8101 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8161 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8221 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8281 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8341 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8401 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8461 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8521 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8581 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8641 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8701 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8761 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8821 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8881 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8941 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     9001 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     9061 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     9121 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     9181 gcgatgtacg ggccagatat
//
