LOCUS       sequence-337251-       10353 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG
            at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 
            170125.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Katrekar D, Xiang Y, Palmer N, Saha A, Meluzzi D, Mali P
  TITLE     Comprehensive interrogation of the ADAR2 deaminase domain for 
            engineering enhanced RNA editing activity and specificity.
  JOURNAL   Elife. 2022 Jan 19;11. pii: 75555. doi: 10.7554/eLife.75555.
  PUBMED    35044296
REFERENCE   2  (bases 1 to 10353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-01-19"; volume: "11. pii: 75555. doi"; pages: " 
            10.7554/eLife.75555"
COMMENT     Sequence Label: MS2-adRNA (3'UAG)
FEATURES             Location/Qualifiers
     source          1..10353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(58..78)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        63..392
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      243..378
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     305..324
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        440..487
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             506..880
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1010..1131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1047..1066)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1101..1120
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1180..1196)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1180..1196)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1193..1215)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1204..1220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1228..1234,1235..1252,1253..1258))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1273..1294
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1411..1428)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1582..2170)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1662..1681)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2341..3132,3133..3201))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2964..2983
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3202..3306)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3381..3400)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3572..3951
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3953..4151
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4106..4126
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4169..4349
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4396..4521
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5014..5247
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5432..5476
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5774..5891
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5942..6190
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5942..5962
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6113..6132
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6193..6211
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        6234..6252
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        6287..6600
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     6519..6539
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        6605..6623
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        6646..6664
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        6728..6939
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6958..6967
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6964..7671
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7109..7127)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7377..7396
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7681..7737
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7738..8763
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    8779..9367
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8832..8852)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9250..9261)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9439..9672
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9698..9715)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9704..9928
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9974..10353,1..49)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10061..10080)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10271..10292
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
       61 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
      121 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
      181 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
      241 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
      301 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
      361 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
      421 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
      481 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
      541 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
      601 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
      661 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
      721 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
      781 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
      841 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
      901 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
      961 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     1021 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     1081 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     1141 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1201 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     1261 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1321 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     1381 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1441 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1501 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1561 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1621 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1681 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1741 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1801 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1861 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1921 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1981 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2041 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2101 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2161 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2221 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2281 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2341 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2401 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2461 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2521 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2581 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2641 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2701 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2761 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2821 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2881 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2941 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3001 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3061 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3121 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3181 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3241 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3301 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     3361 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     3421 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     3481 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     3541 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     3601 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     3661 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     3721 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     3781 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     3841 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     3901 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     3961 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     4021 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     4081 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     4141 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     4201 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4261 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4321 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     4381 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     4441 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     4501 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     4561 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     4621 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     4681 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     4741 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     4801 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     4861 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     4921 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     4981 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     5041 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     5101 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     5161 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     5221 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     5281 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     5341 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     5401 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     5461 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     5521 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     5581 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     5641 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     5701 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     5761 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     5821 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     5881 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta attaaggtac
     5941 cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     6001 gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     6061 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     6121 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     6181 acgaaacacc gaacatgagg atcacccatg tctcttccca gttcgtcttt gcaacatgag
     6241 gatcacccat gtcttttttt cgtactgagt cgcccagtct cagatagatc cgacgccgcc
     6301 atctctaggc ccgcgccggc cccctcgcac agacttgtgg gagaagctcg gctactcccc
     6361 tgccccggtt aatttgcata taatatttcc tagtaactat agaggcttaa tgtgcgataa
     6421 aagacagata atctgttctt tttaatacta gctacatttt acatgatagg cttggatttc
     6481 tataagagat acaaatacta aattattatt ttaaaaaaca gcacaaaagg aaactcaccc
     6541 taactgtaaa gtaattgtgt gttttgagac tataaatatc ccttggagaa aagccttgtt
     6601 tggaacatga ggatcaccca tgtctcttcc cagttcgtct ttgcaacatg aggatcaccc
     6661 atgtcttttt ttgaattcgc tagctaggtc ttgaaaggag tgggaattgg ctccggtgcc
     6721 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     6781 aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     6841 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     6901 aacgttcttt ttcgcaacgg gcttgccgcc agaacacagg accggttcta gagcgctgcc
     6961 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     7021 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     7081 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     7141 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     7201 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     7261 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     7321 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     7381 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     7441 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     7501 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     7561 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     7621 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gggatccggc
     7681 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg
     7741 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     7801 gtgtccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     7861 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     7921 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     7981 gagttcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     8041 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     8101 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     8161 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     8221 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     8281 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     8341 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     8401 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     8461 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     8521 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     8581 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     8641 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     8701 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     8761 taaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8821 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8881 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8941 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     9001 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     9061 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     9121 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     9181 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     9241 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     9301 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     9361 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     9421 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     9481 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9541 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9601 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9661 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     9721 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     9781 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     9841 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     9901 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     9961 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    10021 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    10081 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    10141 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    10201 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    10261 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    10321 tcttttgatt tataagggat tttgccgatt tcg
//
