LOCUS       sequence-337284-        5569 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express ExbD D25N protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Ollis AA, Manning M, Held KG, Postle K
  TITLE     Cytoplasmic membrane protonmotive force energizes periplasmic 
            interactions between ExbD and TonB.
  JOURNAL   Mol Microbiol. 2009 Aug;73(3):466-81. doi: 
            10.1111/j.1365-2958.2009.06785.x. Epub 2009 Jul 16.
  PUBMED    19627500
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2958.2009.06785.x"; journalName: "Mol Microbiol"; 
            date: "2009-08"; volume: "73"; issue: "3"; pages: "466-81"
COMMENT     Sequence Label: pKP1064
FEATURES             Location/Qualifiers
     source          1..5569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(508..525)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      678..764
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      856..883
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        902..993
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(994..1062,1063..1854)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1212..1231)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1896..2351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1983..2002)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2193..2214
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      2462..3050
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2951..2970
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3204..3221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3387..3409
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3547..3566
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atggcaatgc atcttaacga aaacctcgac gataacggcg aaatgcatga tatcaacgtg
       61 acgccgttta tcaacgtgat gttggttctg ctgattatct ttatggtggc ggcaccgtta
      121 gcgacggtag atgtgaaggt gaacttgcct gcttctacca gcacgccgca gccgcgcccg
      181 gaaaaaccgg tttatctgtc ggtgaaggca gacaactcga tgtttatcgg taacgatccg
      241 gtcaccgatg aaacaatgat tacggcgttg aatgcgttaa ccgaaggcaa gaaagacacc
      301 accatcttct tccgagcgga caaaaccgtc gattacgaga cgttgatgaa ggtaatggat
      361 acgctgcatc aggcgggtta cctgaagata ggtctggtcg gcgaagaaac cgccaaagcg
      421 aagtaaccat ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggctg
      481 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
      541 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
      601 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt
      661 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      721 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
      781 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
      841 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
      901 ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      961 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     1021 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1081 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1141 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1201 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     1261 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1321 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1381 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1441 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1501 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1561 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1621 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1681 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     1741 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1801 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1861 gaccaagttt actcatatat actttagatt gatttacgcg ccctgtagcg gcgcattaag
     1921 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     1981 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     2041 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     2101 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     2161 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     2221 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     2281 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     2341 gtttacaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2401 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2461 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2521 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2581 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     2641 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2701 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2761 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2821 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2881 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2941 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3001 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     3061 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     3241 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataggg tcatggctgc
     3301 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     3361 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3421 atcaccgaaa cgcgcgaggc agaaggagat ggcgcccaac agtcccccgg ccacggggcc
     3481 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     3541 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     3601 ggccacgatg cgtccggcgt agaggatctg ctcatgtttg acagcttatc atcgattcag
     3661 cttttcagcc gccgccagaa cgtcgtccgg ctgatgccta aataattcgc cgctgctgtt
     3721 ttatcgccat taaatttctc cagtgcctgt tgtggtgtca gtaagcgtgg agcgggagtt
     3781 ttcgccgact cgcgcgccag ttccggcagt agcagctgca aaaattgcgg cgttaaatcc
     3841 ggcgtcggtt ccacacttaa aaatagcgcc agtcgctcca tcatattgcg cagttcacga
     3901 atattgcccg gccagtcgta gtgcaccagc acggtttcgc ttgcctgtaa tccctggcgt
     3961 aatgcggcag aaaacggggt ggagagcgcc gccagagaca ctttcaaaaa gctttccgcc
     4021 agtggcagaa tatccgccac ccgctcgcgc agtggtggca attgcagacg caaaatactc
     4081 agccgataaa acaggtcacg gcgaaactgc ccttgccgca tatcttcttc cagattgcag
     4141 tgagtggcgc taatgacccg cacatccacc ggaacaggct gatgcccgcc gacgcgggtg
     4201 acctcttttt cttccagcac ccgcagcagc cgggtctgca acggcagcgg catttcgcca
     4261 atctcatcga gaaacagcgt acctccgtgg gcgatttcaa acagcccggc gcgaccgccg
     4321 cgtcgcgagc cggtaaacgc cccttcctca tagccaaaca gttctgcttc cagcagcgat
     4381 tcggcaatcg ccccgcagtt gactgcaaca aacggatgcg actttttgcc ctgtcgcgca
     4441 tcgtggcggg caaaatattc ccgatgaatc gcctgggccg ccagctcttt gcccgtcccc
     4501 gtttccccct caatcaacac cgctgcactg gagcgggcat acagcaaaat agtctgccgt
     4561 acttgttcca tctgtggtga ttgaccgagc atatcgccca gcacgtaacg agttctcagg
     4621 gcgttgcggg tggcatcgtg agtgttatgg cgtaacgaca tgcgcgtcat atccagcgca
     4681 tcgctgaacg cctggcgcac ggtggcggcg gaatagataa aaattccggt cattccggct
     4741 tcttctgcca gatcggtaat cagccccgcg ccgaccaccg cttcggtgcc gttagctttt
     4801 agctcgttaa tctgcccgcg tgcgtcttct tcggtaatgt agctacgttg atcgaggcgc
     4861 aaattaaagg ttttttgaaa cgccaccagt gccggaatag tttcctgata agtgacaacg
     4921 ccgatagaag aggtgagttt tccggctttt gccagtgcct gtaacacatc gtagccgctc
     4981 ggtttaatca aaataactgg cactgacagg cggcttttca ggtacgcgcc gttagatcca
     5041 gcggcgatga tggcgtcaca gcgttcgttt gccagtttct tgtggatgta ggtcaccgct
     5101 ttttcaaagc caagctggat aggggtaatg ttcgccaggt gatcaaactc gaggctgata
     5161 tcgcgaaaca gctcgaacag gcgcgttaca gataccgtcc agataaccgg tttgtcgtca
     5221 ttaagccgtg gtggatgtgc catagcgcac cgcaaagtta agaaaccgaa tattgggttt
     5281 agtcttgttt cataattgtt gcaatgaaac gcggtgaaac attgcctgaa acgttaactg
     5341 aaacgcatat ttgcggatta gttcatgact ttatctctaa caaattgaaa ttaaacattt
     5401 aattttatta aggcaattgt ggcacacccc ttgctttgtc tttatcaacg caaataacaa
     5461 gttgataaca agctagcagg aggaattcac catggacctg gatctggaag ccagcgccgc
     5521 tgcgcatccg gttcgtgtcg cacaaaaatt acgcgcagga taatatccg
//
