LOCUS       sequence-33752-e         897 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Shechner DM, Grant RA, Bagby SC, Koldobskaya Y, Piccirilli JA, 
            Bartel DP
  TITLE     Crystal structure of the catalytic core of an RNA-polymerase 
            ribozyme.
  JOURNAL   Science. 2009 Nov 27. 326(5957):1271-5.
  PUBMED    19965478
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Nov 27. 326(5957):1271-5."
COMMENT     Sequence Label: p11U1ADb
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(350..368)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      364..411
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(586..591,592..608,609..614))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(641..659)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        727..832
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atatggcagt tcccgagacc cgccctaacc acactattta tatcaacaac ctcaatgaga
       61 agatcaagaa ggatgagcta aaaaagtccc tgcacgccat cttctcccgg tttggccaga
      121 tcctggatat cctggtatca cggagcctga agatgagggg ccaggccttt gtcatcttca
      181 aggaggtcag cagcgccacc aacgccctgc gctccatgca gggtttccct ttctatgaca
      241 aacctatgcg tatccagtat gccaagaccg actcagatat cattgccaag atgaaatgag
      301 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      361 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      421 ggaactatat ccggatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc
      481 ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac
      541 acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga
      601 tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt
      661 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
      721 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcnaatat gtatccgctc
      781 atgagacaat aaccctgata aatgcttcaa taatatttga aaagggaaga gtatgagtat
      841 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttt
//
