LOCUS       sequence-337724-       12644 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Fluorescent reporter for CHO cells expression studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12644)
  AUTHORS   Orlova NA, Kovnir SV, Hodak JA, Vorobiev II, Gabibov AG, Skryabin KG
  TITLE     Improved elongation factor-1 alpha-based vectors for stable 
            high-level expression of heterologous proteins in Chinese hamster 
            ovary cells.
  JOURNAL   BMC Biotechnol. 2014 Jun 14;14:56. doi: 10.1186/1472-6750-14-56.
  PUBMED    24929670
REFERENCE   2  (bases 1 to 12644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-06-14"; volume: "14:56. doi"; pages: " 
            10.1186/1472-6750-14-56"
COMMENT     Sequence Label: p1.1-eGFP
FEATURES             Location/Qualifiers
     source          1..12644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      6263..6272
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6269..6271,6272..6274,6275..6988)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6269..6271,6272..6274,6275..6985)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6314..6335)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6575..6594)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6922..6943
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7014..7587
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7037..7589
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7181..7198)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7408..7427
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      7604..7613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7610..8173
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     primer_bind     complement(12474..12491)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gcatctcatc acggtcacgc atggctgcgc ccgcgcagcg ccccccgacc
     1801 cccccccgat ccccggaacg ccagccgcca tccaccgccc gcagcccccg gagcccgcgg
     1861 accccgaccc cccgccgctc gcccgccgtt gcgccgctct ctgcgggggg gctagggggc
     1921 cgcgggggga aggccacgcc ccctccactt tttccaggaa tgcgcggcat gacccgcccc
     1981 ccccatgacc cgccaacacc cccccgatgc cccccggggg tctttcctgg ggggcctttg
     2041 tcagggttgc ctgtgtcacc cacgggcccc ttggccacgc ttgcctgggt caccccggag
     2101 ccccgggtca cgtttgccgg ggtcagccct ggggcgcttc ttcacgctcc attgccatgc
     2161 agaattcact agtgattaga atcccacgtt gtgcatagaa acagatgcag gcaaaacatc
     2221 cacacatata aaacaaaaaa ttaaaaccaa taaaactcct aaacttttgg tctttcttga
     2281 atcttcaatc cctcaggtta tgaaataatc atttatgcag tcaaaaattt gccattcttg
     2341 ttgccaggtg tggtgatgat tcggggaagc agaagcaggc agatctctgt gaatgaggcc
     2401 agcctggtct acaaagtgag tcccaggaca gtcaggcctg ttacacagag aaaccttgaa
     2461 aaaaaaaaga taatatgtac tgttgtatta ccccaatata taaggctaaa ccattagaag
     2521 cacaacactg ttaagtacgg aaaataatat ctagtgtggt acagttacta ctactataat
     2581 acactaatat agctgtggga aactagttcc aaagatgaat tactaaccag tgtttccaag
     2641 gaaataaatg aaagcagaga gattagttct attgctagtg tttcattttc gtatatttct
     2701 tacaatttct cttgttacaa ataggcacta gggtatcaag ataattttaa cgactggctg
     2761 agaaccctag aaaatctctg tgaaaaaggg atttgtgaaa tgagagaggg taatgtggcc
     2821 attatagaaa aggcttttgt gtgccttgca tgcatagacc ctgtgtttga tctcttaaca
     2881 ccctccttga ccagaaaaag cttctgtgga tagaaaatga ttagttatat atacttttag
     2941 ggaaacgtag ttctggattc tttggttaca attaacagaa ttaagtgcaa acaaagccag
     3001 aaacctcctg ataaatgaga aaacctgctt gtagaaggtt gtaaggctct gtaatatagg
     3061 aattaggaga aaagaaacct gtgtggtggg gcacgtctgt aatcccagca ttgggaagta
     3121 gaggtagaag attagaaatc aaaggccagc ctcagcaaca cagtgagttt gaggccaccc
     3181 tgaactacat caggttctgt ctcctttctt tttttttttt ttcttttctt tttttggttt
     3241 ctctgtgtag ttttggagcc tatcctggca ctagctctga agagcaggct ggcctcgaac
     3301 tcagagatca gccagcctct gctgggatta aaggtatgca ccaccaacgc cccaggtttt
     3361 gtctcaaaca aacaaaaata acatcaggag gtggtgagag ggctcagtgg tcacaggcat
     3421 tctctgcaaa gcctgactct gagttggatc ctttagagct acatggttga gggaagagaa
     3481 ctgactcctg gaaggtgtcc tctggtcccc acacatagct atacacagca tgtgcattca
     3541 cacacactaa ataatgctat ttttaaaaaa attaaaaaca acaacagttt gggttgtgaa
     3601 aactagaact agataatagg taagaatcaa gtatcatgta aatttgcttt caactcatcc
     3661 caaaatttgt tttatatttc agtttttttc cttcctagct tgactgtgga gtcttgtccg
     3721 gaagcaaata gttcctttgc agatcccaca tgtggacacc ggacagtagg tcctcaaatg
     3781 ctccttatta ggttggttca ataatatcaa ttgtttgtta ctaggcagtg atgttgtaca
     3841 tctggaggag atctcttgag cccataatca ggttattagg aataaatact ctaaggctaa
     3901 aaatgtagct tagtgataag agtgcttgcc tggtgtgctg agaccctcgg ttccatctcc
     3961 acaaccccat attccattac aaaatacctt ttcaccgtcc ctagcattaa gaaacaaaac
     4021 aacaaagaag tttttctttc ttctgagatc ctgcccggag aggcatttaa aactggccag
     4081 ggccaaaaaa aaaaaaaaaa gaaaaaaaag aaaagaaaac aggctagggc cggcatggtg
     4141 gcgcacgcct ttaatcccag cacgcaggag gcagaggcag ggcggatctc tgtgagtttg
     4201 aggtcagcct ggtctaccta gtgagtttca gggcacccag ggctaaagag actgtctcaa
     4261 aaacaaaaca gccacacaat cagaaccaca gcaaaacgca gttatgatcc ttggaactgt
     4321 aggaatgaca agcatttaaa taataggacg agccattttt gagaagctct gatttcacaa
     4381 gtgtcaggga tgggctctgg gcgagtaaga ttgctaatgc tggcctctaa atgagaccac
     4441 gtggagttga ttagattctt ttcatgttcc tcgtgctcta tcaaataact gtacccaaat
     4501 acacacacac acacacacac acaatgcgcg cacacacaaa atcctttttt agcttaagaa
     4561 gcccagaatc agaagtaaag ctaactgtgg gacttaagta ttattctgaa cggaactccc
     4621 agggcgtgaa gcgcgcttca ggcttccaga gaagcagctg gcgctggatg gaatgaacca
     4681 agaggccagc acaggggcag atccgtcgag ctctcggcca ccgagctgag cccttaggtt
     4741 ctggggctgg gaagggtccc taggattgtg cacctctccc gcgggggaca agcaggggga
     4801 tggcggggct gacgtcggga ggtggcctcc acgggaaggg acacccggat ctcgacacag
     4861 ccttggcagt ggagtcagga agggtaggga cagattctgg acgccctctt ggccagctcc
     4921 tcaccgcccc acccccgact ggagccgaga gtaattcata caaaaggagg gatcgccttc
     4981 gcccctggga atcccaggga ccgtcgctaa attctggccg gcctcccagc ccggaaccgc
     5041 tgtgcccgcc cagcgcggcg ggaggagcct gcgcctaggg cggatcgcgg gtcggcggga
     5101 gagcacaagc ccacagtccc cggcggtggg ggaggggcgc gctgagcggg ggcccgggag
     5161 ccagcgcggg gcaaactggg aaagtggcgt cgtgtgctgg ctccgccctc ttcccgaggg
     5221 tgggggagaa cggtataaaa gtgcggtagt cgcgttggac gttctttttc gcaacgggtt
     5281 tgccgtcaga acgcaggtga gtggcgggtg tggcctccgc gggcccgggc tccctccttt
     5341 gagcggggtc gggccgccgt gcgggtgtcg tcggccgggc ttctctgcga gcgttcccgc
     5401 cctggatggc gggctgtgcg ggagggcgag ggggggaggc ctggcggcgg ccccggagcc
     5461 tcgcctcgtg tcgggcgtga ggcctagcgt ggcttccgcc ccgccgcgtg ccaccgcggc
     5521 cgcgctttgc tgtctgcccg gctgccctcg attgcctgcc cgcggcccgg gccaacaaag
     5581 ggagggcgtg gagctggctg gtagggagcc ccgtagtccg catgtcgggc agggagagcg
     5641 gcagcagtcg ggggggggac cgggcccgcc cgtcccgcag cacatgtcca acgccgcctg
     5701 gacgggtagc ggcctgtgtc ctgataaggc ggccgggcgg tgggttttag atgccgggtt
     5761 caggtggccc cgggtcccgg cccggtctgg ccagtacccc gtagtggctt agctccgagg
     5821 agggcgaggc ccgcccgccc ggcaccagtt gcgtgcgcgg aaagatggcc gctcccgggc
     5881 cctgtagcaa ggagctcaaa atggaggacg cggcagcccg gcggagcggg gcgggtgagt
     5941 cacccacaca aaggaagagg gccttgcccc tcgccggccg ctgcttcctg tgaccccgtg
     6001 gtgtaccggc cgcacttcag tcaccccggg cgctctttcg gagcaccgct ggcctccgct
     6061 gggggagggg atctgtctaa tggcgttgga gtttgctcac atttggtggg tggagactgt
     6121 agccaggcca gcctggccat gaaagtaatt cttggaattt gcccattttg agtttggagc
     6181 gaagctgatt gacaaagctg cttagccgtt caaaggtatt cttcgaactt tttttttaag
     6241 gtgttgtgaa aacctcgagg ccgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     6301 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     6361 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     6421 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     6481 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     6541 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     6601 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     6661 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     6721 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     6781 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     6841 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     6901 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     6961 tctcggcatg gacgagctgt acaagtaagc tagcggccgc gcagttaacg ccgcccctct
     7021 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     7081 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     7141 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     7201 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     7261 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     7321 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     7381 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     7441 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc
     7501 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     7561 gttttccttt gaaaaacacg atgataatat ggccacaaga tctgccacca tggttcgacc
     7621 attgaactgc atcgtcgccg tgtcccaaaa tatggggatt ggcaagaacg gagacctacc
     7681 ctggcctccg ctcaggaacg agttcaagta cttccaaaga atgaccacaa cctcttcagt
     7741 ggaaggtaaa cagaatctgg tgattatggg taggaaaacc tggttctcca ttcctgagaa
     7801 gaatcgacct ctaaaggaca gaattaatat agttctcagt agagaactca aagaaccacc
     7861 acgaggagct cattttcttg ccaaaagttt ggatgatgcc ttaagactta ttgaacaacc
     7921 ggaattggca agtaaagtag acatggtttg gatagtcgga ggcagttctg tttaccagga
     7981 agccatgaat caaccaggcc acctcagact ctttgtgaca aggatcatgc aggaatttga
     8041 aagtgacacg tttttcccag aaattgattt ggggaaatat aaacttctcc cagaataccc
     8101 aggcgtcctc tctgaggtcc aggaggaaaa aggcatcaag tataagtttg aagtctacga
     8161 gaagaaagac taatctagca tattacccct aacacctgcc accccagtct taatcagtgg
     8221 tggaagaacg gtctcagaac tgtttgtctc aattggccat ttaagtttaa tagtgaaaga
     8281 ctggttaatg ataacaatgc atcggaaaac cttcaggagg aaaggagaat gttttgtgga
     8341 acatttttgt gtgtgtggca gttttaagtt attagttttc aaaatcagta ctttttaatg
     8401 gaaacaactt gaccaaaaat ctgtcacaga attttgagac ccattaaaat acaagtttaa
     8461 tgagaagtct gtctctgtta atgctgaagt cattactaag tgcttagctt agcaaggtat
     8521 gtggatgccc atttgtgttc caagggattg gactgttcat caggacccag agctgagttt
     8581 caagggctca agagatggct tattacctgt gggtgtcttg aaggttctgg ttgggacaaa
     8641 ttaggaatgt ttttggcaga catggtgact acccttcatc tgggtgagtt cagttgattt
     8701 gtcttgagcc tttggggttt acacaagtaa atgacatcat acagttagtg tattgttagt
     8761 gaatattaat atatgaggca ggctttgctc tagcaatttt agaactagtt ttcaggaaag
     8821 ggttcatctt gtgcattgga tgtttgattc tatcacttag agtttaaact gaaagtgctc
     8881 aagaggtttt atttaggctg ggatataaat aagcctttct gtagcttgta atggtatcag
     8941 gaatttaaaa ggccatctgg ggcacaaaga ttaagcagaa aaggtagaag gtgagattgg
     9001 gggactttga gtacttcaca cactttaatg tgtgagtgct ttagtgcata tagtacaact
     9061 gccagataag ggcatccaca tctgattgtt tggaaggcac cttgtggttt ctgggaattc
     9121 agaattggga gaaaaagtgc tcccaaccgc tgaagccctt ggtaatctgc agggtgttta
     9181 tttagcagga gataaggaca aaaagttata gtgtggagtt ggttgagttg gtagatgtca
     9241 ttacaacagg tggtcttaaa ttgggttagg agtcactttg aaatacctgg gccataagca
     9301 aagtggcatt ttcacctttc aggagaaact ggtacactta tccattctat agtgcatgct
     9361 tgttcaattg ggctgatgac taaaccggtg actaaaggtt tgtcagtata aatggatggg
     9421 ttgtaggcag acggtgagga attactatac ctgcaaggag tcattgcctg atctgcctgg
     9481 aaaggggcag gattgagtct cagaaggtgt acaccatagg atatggaaaa atttgtcacg
     9541 cctagcattc aacttagtgg tgtagcgcca cctactggca ctttaaaagc ttagcataga
     9601 ggagcatgtg tgttaggagc tcggatggga tccagggcct caaggtttgc atgtaaataa
     9661 aagcccttta ccaaattaac tacataccag catacatcag tcctttagtg ttgaaaaaca
     9721 gaagtgaaag ctaatatata tagtgcttgc tttatttaag tctagctgat tacgtgtttg
     9781 gttgccagtg tgactagtct ggagttgaat ttgtcctcag acacgtaaaa tggaatttgg
     9841 gattcacaac actctagtat gagggaccta atggcctgta ccaggcacaa acgtgtctat
     9901 aaactacaca aaacgaagga atttacagga attaggaagg tattcttaac attaaaacat
     9961 tatgggcatt ttaaaaaaag ctttgacagg atttctttgt catggctgtc ctggagctag
    10021 ttgtgtagac caggctgggc tgaaatcttg tctgcctgcc tggcttggac acttttttat
    10081 tatgtataca acattctgct tccatgtata tctgcacatt agaagacggc accagatctc
    10141 ctaatggatg gttgtgagcc accatgtggt tgctgggaat tgaactcagg acctctggaa
    10201 gagcagtgct cttaacctct gagccatctc cagccccagc ttgggcacat ttttaatggc
    10261 tgggaaatca aaccccctag gccttctgtc agtaatgaag ggcttttggc taccgagagt
    10321 aggatttaag gttattcgga gctgcaggtc tgcctcagtg caggtttggg agtccagcat
    10381 cttagaaaat gcagtgaagc caagctgagc tatattttgt ttaaaaaaaa aataagtggg
    10441 taaagtgctg ctgagcctga tgaccaagct gggacacaag tagaagaaca taggccaatg
    10501 ctctatatta aaagcatggg tcatttttaa tgctcttgag aaggctatgc ctacactact
    10561 ctcagccacc gcagcgtgtt taaattaaac tagtttggaa attttctttg ggggtaagct
    10621 atttaaccta gtgccttggc aggtatacta ctgaactctc ctcctcattc ctttttgttt
    10681 tttaagaatt tcagtcaggc tcaggcagcc cttaaacttg tgattaagcc tgagaacagt
    10741 tacgattatg agcctattag tataccgatc aatatgtgaa tttttttggg atgggggtca
    10801 ggcctccctg cctcccaaat actgggacta aaggctgcac caccacaacc tggctcttga
    10861 aatacttttc tacatttttt ggggggcatg ggtgggagag cagggtttct ctgtattagc
    10921 cctggctctc ctggaactct gtagaccagg ctattcttga gctcagatta gcctgtctct
    10981 gcctcctaaa ttctgggatt aaaggtgtgt gctactgctg cctggctaca aagacatttt
    11041 tttttttctt aaatttaaaa acaaaagtgg ttcttttaga agggtggttg gtgttggcac
    11101 atactccaag cactcaggtt ttgagtttgt cccaggaatg aagactgcat tactgccgcc
    11161 cctccctggt aagggctaca cagagaaatc ctatttggag cctgtcctgg taactcgctc
    11221 tgtagaccag gctggcctcg aactcaagag aaccacctgc ctctgaatgc tggtattaag
    11281 ggcaggcacc accaacaccc agcctaaaaa atgtcttttt tttaaagatt tttttttttt
    11341 ttttacagaa taaacattct gtttacaata ttctgcttct atgtatatct gcacactaga
    11401 agagggcacc cgatctcata atggatggtt gtgagccacc aagtggttgc tgggaattga
    11461 actcagaacc tctggaagag cagtcagtgc tcttaacctc tgagccatct ctccagcccc
    11521 taaaaatggc tcttgagata gggtctcaag tagtttgaga ctgagttggc tatataaaca
    11581 aggctggcac atagcaccat gtacagctgg gtttagttta catggggtgt ttttgtctct
    11641 ggaggcagga ggatcatttg agcataggga gttaatagtg aggtcatgtt ttatctactc
    11701 ttctgaattg agaactaagc tgatgcaaag caagtttgac tgaagaagtc cagtttatga
    11761 gaacaagggt ggaaactaat gtgtcaaaga tggccttgca tgtgttttag atgatgaccc
    11821 agtcacttgg gaattactgg atgtgtaaga cctatatctt gacaggagtg aacagtgtct
    11881 tataggtcct atatgaaaga aatgagacat acccattttg tttcccctaa gaattcactt
    11941 ttcctaacct ggttcatgct atttaggtta ttttacttgc aaatcctagg gtgctccctt
    12001 acccagtatt gcttatgtgg caccaaagtc actcactccc atgatttgca agtctctggg
    12061 aacttccatg acaacctaga atagcaactc aaatacattt tctcagtacc aattttgaag
    12121 aaaaaatatt ttgcaaaata gctgtatgga tgggtactaa atagtgaggt tatctccaga
    12181 aggcctatga agaattaagg ttgagttcag ttgagttcag cagcaagttt aaggttcatc
    12241 catttttgta cagtgttttc ctattacggt aagtgttttg cctgcaggaa tatctgtacc
    12301 acatgcttgc ctggtaccta tatcggccag aagagggctt tggatcctct ggacttgaat
    12361 tacagatggg tattagccac catttaggtg ctgggaattg aaaccaagtc ctctggaaga
    12421 acagcaagtg actcgacgtc cggagctctg gcgcgccatg gctcttccgc ttcctcgctc
    12481 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    12541 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    12601 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtt
//
