LOCUS       sequence-337808-        8369 bp    DNA     circular SYN 15-APR-2025
DEFINITION  inducible expression of mCherry fused to C-tag TNF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Pinci F, Gaidt MM, Jung C, Kuut G, Jackson MA, Bauernfried S, 
            Hornung V
  TITLE     C-tag TNF: a reporter system to study TNF shedding.
  JOURNAL   J Biol Chem. 2020 Dec 25;295(52):18065-18075. doi: 
            10.1074/jbc.RA120.015248. Epub 2020 Oct 20.
  PUBMED    33082141
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA120.015248"; journalName: "J Biol Chem"; date: 
            "2020-12-25- 25"; volume: "295"; issue: "52"; pages: "18065-18075"
COMMENT     Sequence Label: pLI_C-tag TNF linker mCherry
FEATURES             Location/Qualifiers
     source          1..8369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             543..1250
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(688..706)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     956..975
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        1971..2481
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2400..2418
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(2482..3099,3100..3105,3106..3141,3142..3144,3145..3180,
                     3181..3183,3184..3219,3220..3228)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3268..3288)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3686..3697)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3902..4135
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4207..4328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4244..4263)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4298..4317
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4368..4503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4503..4574
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4575..4643,4644..5435)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4793..4812)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5606..6194
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6095..6114
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6348..6365
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6482..6503
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6518..6523,6524..6541,6542..6548)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6556..6572
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6561..6583
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6580..6596
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6580..6596
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6615..6635
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6617..6635
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6663..6889
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6890..7070
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7117..7242
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7735..7968
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8153..8197
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
      601 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      661 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
      721 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
      781 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
      841 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
      901 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
      961 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     1021 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     1081 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     1141 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     1201 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag gggggaggcg
     1261 gaagtagcac tgaaagcatg atccgggacg tggagctggc cgaggaggcg ctccccaaga
     1321 agacaggggg gccccagggc tccaggcggt gcttgttcct cagcctcttc tccttcctga
     1381 tcgtggcagg cgccaccacg ctcttctgcc tgctgcactt tggagtgatc ggcccccaga
     1441 gggaagagtt ccccagggac ctctctctaa tcagccctga gccggaagct gtcagatcat
     1501 cttctcgaac cccgagtgac aagcctgtag cccatgttgt agcaaaccct caagctgagg
     1561 ggcagctcca gtggctgaac cgccgggcca atgccctcct ggccaatggc gtggagctga
     1621 gagataacca gctggtggtg ccatcagagg gcctgtacct catctactcc caggtcctct
     1681 tcaagggcca aggctgcccc tccacccatg tgctcctcac ccacaccatc agccgcatcg
     1741 ccgtctccta ccagaccaag gtcaacctcc tctctgccat caagagcccc tgccagaggg
     1801 agaccccaga gggggctgag gccaagccct ggtatgagcc catctatctg ggaggggtct
     1861 tccagctgga gaagggtgac cgactcagcg ctgagatcaa tcggcccgac tatctcgact
     1921 ttgccgagtc tgggcaggtc tactttggga tcattgccct gtgaggatcc ggggttgggg
     1981 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     2041 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     2101 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     2161 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     2221 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     2281 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     2341 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     2401 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     2461 atcaccgacc tctctcccca gatgtctaga ctggacaaga gcaaagtcat aaacggagct
     2521 ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag
     2581 ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat
     2641 gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag
     2701 tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat
     2761 cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg
     2821 gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct
     2881 ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta
     2941 gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca
     3001 attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc
     3061 atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac
     3121 gattttgact tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg
     3181 cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaact aagtaaggat
     3241 cgatccaaga tatcgtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3301 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3361 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3421 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3481 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3541 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3601 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     3661 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3721 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3781 ctccctttgg gccgcctccc cgcctgtttc gcctcggcgt ccggactaga ggtaccttta
     3841 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     3901 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     3961 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4021 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     4081 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     4141 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     4201 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4261 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4321 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     4381 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4441 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4501 gcctttcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4561 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4621 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4681 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4741 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4801 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4861 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4921 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4981 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5041 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5101 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5161 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5221 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5281 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5341 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5401 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5461 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5521 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5581 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5641 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5701 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     5761 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5821 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5881 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5941 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6001 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6061 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6121 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6181 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6241 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6301 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6361 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6421 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6481 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6541 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6601 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     6661 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     6721 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     6781 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     6841 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     6901 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6961 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7021 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7081 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7141 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7201 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7261 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7321 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7381 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7441 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7501 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7561 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     7621 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7681 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     7741 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     7801 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     7861 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     7921 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     7981 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8041 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8101 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8161 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8221 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8281 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8341 tcagacccac ctcccaaccc cgaggggac
//
