LOCUS       sequence-337857-        7907 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Constitutive overexpression of the 3'UTR of the human ARID3A gene 
            after a GFP, to evaluate miRNA binding. Regions not containing 
            miR-125b or let-7c binding regions not included..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich
            S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, 
            Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, 
            Klusmann JH
  TITLE     The megakaryocytic transcription factor ARID3A suppresses leukemia 
            pathogenesis.
  JOURNAL   Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 
            10.1182/blood.2021012231.
  PUBMED    34570885
REFERENCE   2  (bases 1 to 7907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2021
            Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231."
COMMENT     Sequence Label: SIN40C.EFS.eGFP.ARID3A-3'UTR-WT
FEATURES             Location/Qualifiers
     source          1..7907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        169..395
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             396..576
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    623..748
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1241..1474
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1659..1703
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1916..2033
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2103..2314
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2331..2340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2337..2339,2340..2342,2343..3056)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2337..2339,2340..2342,2343..3053)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2382..2403)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2643..2662)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2990..3011
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3265..3853
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3318..3338)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3736..3747)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3925..4158
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4236..4357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4273..4292)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4327..4346
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4397..4532
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4552..4571)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4553..4571)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4581..4598)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4581..4597)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4590..4612)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4739..5194
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4826..4845)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5036..5057
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5220..5324
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5325..5393,5394..6185)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5543..5562)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6356..6944
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6845..6864
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7098..7115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7232..7253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7268..7273,7274..7291,7292..7298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7306..7322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7311..7333
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7330..7346
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7330..7346
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7365..7385
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7367..7385
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        7430..7809
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        join(7866..7907,1..150)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(join(7889..7907,1..2))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
       61 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
      121 gcatctcaat tagtcagcaa ccatagtccc ttaagaggcg cgccttaatg tagtcttatg
      181 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      241 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      301 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      361 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      421 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      481 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      541 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      601 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      661 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      721 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
      781 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
      841 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
      901 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
      961 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1021 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1081 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1141 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1201 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1261 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1321 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1381 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1441 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1501 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1561 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1621 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1681 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1741 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     1801 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     1861 caaccccgag gggacaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     1921 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     1981 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2041 attacaggga cagcagagat ccactttggc gccggctcga gttggctccg gtgcccgtca
     2101 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2161 atccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2221 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2281 tctttttcgc aacgggtttg ccgccagaac acaggtgtcg tgacggatcc gccaccatgg
     2341 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     2401 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     2461 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     2521 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     2581 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     2641 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     2701 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     2761 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     2821 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     2881 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     2941 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3001 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaatgtt
     3061 tacctcatac atcgtgttcc ttcagggaaa ctggtcttga aaaagcaccc aggcatcttg
     3121 tctcagggat gaatgtggac ctcactcagg gtgaggatgc ccctgagtct aggttcttct
     3181 cggatctcag ggccgtggac tctgtctacc tcaggtcttt actactctct acctctgact
     3241 aagcgcagcc cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     3301 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3361 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3421 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3481 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3541 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3601 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3661 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3721 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3781 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3841 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     3901 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     3961 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4021 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4081 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4141 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     4201 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     4261 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4321 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     4381 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4441 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4501 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     4561 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4621 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4681 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     4741 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4801 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4861 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4921 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4981 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5041 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5101 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5161 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     5221 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5281 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5341 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5401 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5461 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5521 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5581 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5641 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5701 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5761 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5821 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5881 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5941 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6001 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6061 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6121 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6181 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6241 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6301 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6361 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6421 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6481 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6541 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6601 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6661 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6721 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6781 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6841 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6901 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6961 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7021 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7081 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7141 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     7201 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     7261 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7321 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     7381 aaagggaaca aaagctggag ctgcaagcgg taccgtcgac ctgcaggttg acattgatta
     7441 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     7501 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     7561 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     7621 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     7681 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     7741 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     7801 attaccatgg cgcgccatgc agggcctgaa ataacctctg aaagaggaac ttggttaggt
     7861 accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagg
//
