LOCUS       sequence-338282-        8102 bp    DNA     circular SYN 14-APR-2025
DEFINITION  3rd gen lentiviral backbone for cloning and expression of new shRNA 
            sequences. Uses eGFP for selection. Please see cloning information 
            for suggested restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8102)
  TITLE     pLKO.3G variant (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLKO.3G_X7
FEATURES             Location/Qualifiers
     source          1..8102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    129..150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(165..170,171..188,189..195)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    203..219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     208..230
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     227..243
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     227..243
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     262..282
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        264..282
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        310..536
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             537..717
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    764..889
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1382..1615
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1800..1844
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2361..2609
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2361..2381
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2532..2551
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2701..2818
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2867..3377
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3296..3314
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      3393..3402
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3399..3401,3402..3404,3405..4118)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3399..3401,3402..3404,3405..4115)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3444..3465)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3705..3724)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4052..4073
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4137..4725
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4190..4210)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4608..4619)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4728..4744)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4729..4745)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4930..5163
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5235..5356
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5272..5291)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5326..5345
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5396..5531
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5551..5570)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5552..5570)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5580..5597)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5580..5596)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5589..5611)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5738..6193
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5825..5844)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6035..6056
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6219..6323
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6324..6392,6393..7184)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6542..6561)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7355..7943
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7844..7863
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(8097..8102,1..12)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
       61 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      121 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      181 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
      241 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
      301 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
      361 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
      421 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
      481 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
      541 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      601 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      661 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
      721 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
      781 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
      841 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
      901 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
      961 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     1021 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     1081 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     1141 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     1201 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     1261 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1321 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1381 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1441 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1501 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1561 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1621 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1681 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     1741 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     1801 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     1861 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     1921 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     1981 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2041 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
     2101 atcgatcacg agactagcct cgaccacgac gcaatggaga aagacggaga gcgccaacgg
     2161 cgtccatctc gaaggagtcg ccagcgataa ccggagtagt tgaaatggta ataagacgac
     2221 caatctgacc agcaaggaag ccaagatggg aaaggtcatg cggcatacgc tcggcgccag
     2281 tttgaatatt agacataatt tatcctcaag taagggggta cacgcgtcag tcgagtcctg
     2341 tcgacaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat ttgcatatac
     2401 gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag
     2461 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     2521 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
     2581 tatatatctt gtggaaagga cgaaacaccg gtccgcaggt atgcttgcgt gaattccacc
     2641 tcgagctact taattaaacc agttttcgac ctcgagacaa atggcagtat tcatccacaa
     2701 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     2761 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     2821 ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc
     2881 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     2941 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     3001 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     3061 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     3121 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     3181 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     3241 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     3301 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     3361 ccgacctctc tccccagggg gatccaacgg tcgccaccat ggtgagcaag ggcgaggagc
     3421 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3481 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     3541 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     3601 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3661 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     3721 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3781 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     3841 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     3901 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     3961 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     4021 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     4081 ccgggatcac tctcggcatg gacgagctgt acaagtaaga tatcaagctt atcgataatc
     4141 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4201 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4261 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4321 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4381 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4441 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4501 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     4561 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4621 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4681 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     4741 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     4801 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     4861 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     4921 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     4981 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5041 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     5101 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     5161 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     5221 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5281 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5341 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     5401 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5461 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5521 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     5581 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     5641 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     5701 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     5761 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5821 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5881 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5941 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6001 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6061 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6121 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6181 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6241 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6301 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6361 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6421 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6481 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6541 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6601 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6661 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6721 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6781 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6841 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6901 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6961 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7021 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7081 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7141 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7201 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7261 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7321 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7381 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7441 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7501 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7561 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7621 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7681 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7741 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7801 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7861 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7921 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7981 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8041 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8101 ag
//
