LOCUS       sequence-338426-        8118 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Equipped with an autoinducible promoter. Possible sucrose selection.
            Works in a general host and in a general medium. Real-time 
            monitoring of the protein production. 8xHis at the N-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Chae YK, Kim H
  TITLE     Development of an Autoinducible Plasmid for Recombinant Protein 
            Production.
  JOURNAL   Protein Pept Lett. 2021;28(12):1398-1407. doi: 
            10.2174/0929866528666211105113750.
  PUBMED    34749604
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Pept Lett."; date: "2021"; volume: "28(12):1398-1407. doi"; pages: "
            10.2174/0929866528666211105113750"
COMMENT     Sequence Label: pVP65KR-gb-SacB(-)
FEATURES             Location/Qualifiers
     source          1..8118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(20..106)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(139..849)
                     /codon_start=1
                     /product="tobacco vein mottling virus NIa protease 
                     (Nallamsetty et al., 2004)"
                     /label=TVMV protease
                     /translation="MSKALLKGVRDFNPISACVCLLENSSDGHSERLFGIGFGPYIIAN
                     QHLFRRNNGELTIKTMHGEFKVKNSTQLQMKPVEGRDIIVIKMAKDFPPFPQKLKFRQP
                     TIKDRVCMVSTNFQQKSVSSLVSESSHIVHKEDTSFWQHWITTKDGQCGSPLVSIIDGN
                     ILGIHSLTHTTNGSNYFVEFPEKFVATYLDAADGWCKNWKFNADKISWGSFTLVEDAPE
                     DDFMAKKTVAAIMD"
     RBS             855..863
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(join(873..898,899..904,905..921,922..927,
                     928..946))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    903..921
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    928..946
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             1014..1025
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1032..2135
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MGKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKL
                     EEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGK
                     LIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLI
                     AADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKG
                     ETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEF
                     LENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSA
                     FWYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2109..2132
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2847..2867
                     /codon_start=1
                     /product="tobacco vein mottling virus (TVMV) NIa protease 
                     recognition and cleavage site
                     "
                     /label=TVMV site
                     /translation="ETVRFQS"
     CDS             2868..2891
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             join(2907..2993,2994..4325)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     complement(4442..4460)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     protein_bind    complement(4474..4498)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4584..4670
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4763..5845)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5807..5826
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5846..5923)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(5968..5990)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6076..6216
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6131..6150
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6231..6248)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6391..6979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6471..6490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7156..7950)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7267..7286)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7877..7896
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cgtacgtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
       61 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctctagcacg
      121 cgtatcattg gatctttatt agtccatgat ggcggcaaca gtttttttgg ccatgaagtc
      181 atcttccggc gcatcttcaa ccagggtaaa ggaaccccag ctgattttat ccgcgttgaa
      241 tttccagttt ttgcaccaac catccgcggc atcgagataa gtcgccacga atttttccgg
      301 aaattccacg aagtagttgc taccgttggt ggtatgagtc aggctgtgga tgcccagaat
      361 gttgccatca atgatggaaa ctagtgggct gccacactgg ccatctttag tggtgatcca
      421 gtgctgccag aaagaagtgt cttctttatg cacaatgtga gaggattcag acaccaggct
      481 ggacacgctt ttctgctgaa agttggtgga caccatgcac acacgatctt tgatggtcgg
      541 ctgacggaat ttcagtttct gcgggaacgg cgggaagtct ttagccattt tgataacgat
      601 aatgtcacgg ccttcaaccg gtttcatctg cagctgggta gagtttttga ctttgaattc
      661 accgtgcatg gttttgatgg tcagttcgcc attgttacga cgaaacagat gctggttggc
      721 aatgatatac gggccaaaac caatgccaaa cagacgttca ctatgaccat ccgaggagtt
      781 ttccagcagg catacgcaag cagagatcgg attaaaatcg cgcacgccct tcagcaaagc
      841 cttagacata tcttacctcc ttaaatcaat tcggtcagtg cgtcctgctg atgtgctcag
      901 tatctctatc actgataggg atgtcaatct ctatcactga tagggactcg agaaagcctt
      961 gcttccattg cggatgtaaa agcggctagt atttatttca cacagaattc attaaagagg
     1021 agaaattaac catgggtaaa atcgaagaag gtaaactggt aatctggatt aacggcgata
     1081 aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag
     1141 tcaccgttga gcatccggat aaactggaag agaaattccc acaggttgcg gcaactggcg
     1201 atggccctga cattatcttc tgggcacacg accgctttgg tggctacgct caatctggcc
     1261 tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg
     1321 atgcggtacg ttacaacggc aagctgattg cttacccgat cgctgttgaa gcgttatcgc
     1381 tgatttataa caaagatctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc
     1441 tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt
     1501 acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca
     1561 agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc
     1621 tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag
     1681 ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca
     1741 tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat
     1801 ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc cgccagtccg aacaaagagc
     1861 tggcaaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata
     1921 aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc
     1981 cacgtattgc cgccactatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc
     2041 agatgtccgc tttctggtat gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc
     2101 agactgtcga tgaagccctg aaagacgcgc agactatggt ttccaagggc gaggaggata
     2161 acatggctat cattaaagag ttcatgcgct tcaaagttca catggagggt tctgttaacg
     2221 gtcacgagtt cgagatcgga ggcgaaggcg agggccgtcc gtatgaaggc acccagaccg
     2281 ccaaactgaa agtgactaaa ggcggcccgc tgccttttgc gtgggacatc ctgagcccgc
     2341 aatttatgta cggttctaaa gcgtatgtta aacacccagc ggatatcccg gactatctga
     2401 agctgtcttt tccggaaggt ttcaagtggg aacgcgtaat gaattttgaa gatggtggtg
     2461 tcgtgaccgt cactcaggac tcctccctgc aggatggcga gttcatctat aaagttaaac
     2521 tgcgtggtac taattttcca tctgatggcc cggtgatgca gaaaaagacg atgggttggg
     2581 aggcgtctag cgaacgcatg tatccggaag atggtgcgct gaaaggcgaa attaaacagc
     2641 gcctgaaact gaaagatggc ggccattatg acgctgaagt gaaaaccacg tacaaagcca
     2701 agaaacctgt gcagctgcct ggcgcgtaca atgtgaatat taaactggac atcacctctc
     2761 ataatgaaga ttatacgatc gtagagcaat atgagcgcgc ggagggtcgt cattctaccg
     2821 gtggcatgga tgagctgtac aaaggggaaa ccgtgcgttt ccagtctcat caccatcatc
     2881 accatcacca tgcatccgcg atcgccatga acatcaaaaa gtttgcaaaa caagcaacag
     2941 tattaacctt tactaccgca ctgctggcag gaggcgcaac tcaagcgttt gcgaaagaaa
     3001 cgaaccaaaa gccatataag gaaacatacg gcatttccca tattacacgc catgatatgc
     3061 tgcaaatccc tgaacagcaa aaaaatgaaa aatatcaagt tcctgaattc gattcgtcca
     3121 caattaaaaa tatctcttct gcaaaaggcc tggacgtttg ggacagctgg ccattacaaa
     3181 acgctgacgg cactgtcgca aactatcacg gctaccacat cgtctttgca ttagccggag
     3241 atcctaaaaa tgcggatgac acatcgattt acatgttcta tcaaaaagtc ggcgaaactt
     3301 ctattgacag ctggaaaaac gctggccgcg tctttaaaga cagcgacaaa ttcgatgcaa
     3361 atgattctat cctaaaagac caaacacaag aatggtcagg ttcagccaca tttacatctg
     3421 acggaaaaat ccgtttattc tacactgatt tctccggtaa acattacggc aaacaaacac
     3481 tgacaactgc acaagttaac gtatcagcat cagacagctc tttgaacatc aacggtgtag
     3541 aggattataa atcaatcttt gacggtgacg gaaaaacgta tcaaaatgta cagcagttca
     3601 tcgatgaagg caactacagc tcaggcgaca accatacgct gagagatcct cactacgtag
     3661 aagataaagg ccacaaatac ttagtatttg aagcaaacac tggaactgaa gatggctacc
     3721 aaggcgaaga atctttattt aacaaagcat actatggcaa aagcacatca ttcttccgtc
     3781 aagaaagtca aaaacttctg caaagcgata aaaaacgcac ggctgagtta gcaaacggcg
     3841 ctctcggtat gattgagcta aacgatgatt acacactgaa aaaagtgatg aaaccgctga
     3901 ttgcatctaa cacagtaaca gatgaaattg aacgcgcgaa cgtctttaaa atgaacggca
     3961 aatggtacct gttcactgac tcccgcggat caaaaatgac gattgacggc attacgtcta
     4021 acgatattta catgcttggt tatgtttcta attctttaac tggcccatac aagccgctga
     4081 acaaaactgg ccttgtgtta aaaatggatc ttgatcctaa cgatgtaacc tttacttact
     4141 cacacttcgc tgtacctcaa gcgaaaggaa acaatgtcgt gattacaagc tatatgacaa
     4201 acagaggatt ctacgcagac aaacaatcaa cgtttgcgcc gagcttcctg ctgaacatca
     4261 aaggcaagaa aacatctgtt gtcaaagaca gcatccttga acaaggacaa ttaacagtta
     4321 acaaattgtt taaactgaat tcgagctcgg tacccgggga tcctctagag tcgacctgca
     4381 ggcatgcaag ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     4441 accgctgagc aataactagc aaactcgttt ctcgttcagc tttcttgtac aaagtggtga
     4501 tggcgcgcct gtaggacgtc gacggtacca tcgatacgcg ttcgaagctt cgcctggggt
     4561 aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     4621 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccctcta
     4681 gattacgtgc agtcgatgat aagctgtcaa acatgagaat tgtgcctaat gagtgagcta
     4741 acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4801 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg
     4861 tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct
     4921 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga
     4981 tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg
     5041 agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca
     5101 tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg
     5161 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt
     5221 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta
     5281 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca
     5341 gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat
     5401 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat
     5461 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg
     5521 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt
     5581 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg
     5641 aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg
     5701 gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt
     5761 ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga
     5821 catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct
     5881 atcatgccat accgcgaaag gttttgcgcc attcgatggt gtcggaacct agagctgcct
     5941 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     6001 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     6061 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     6121 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     6181 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     6241 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     6301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6361 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6421 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6481 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6541 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6601 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6661 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6721 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6781 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     6841 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6901 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6961 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7021 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7081 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7141 gtctgacagc ctaggtcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     7201 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
     7261 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
     7321 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
     7381 atcgccatga gtcacgacga gatcctcacc atccggcata cgcgccttga gcctggcgaa
     7441 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
     7501 ggcttccatc cgagtgcgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     7561 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     7621 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
     7681 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     7741 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt
     7801 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     7861 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     7921 acctgcgtgc aatccatctt gttcaagcat gcgaaacgac cgtcatcctg tctcttgatc
     7981 agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
     8041 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattcttttg aagctcacgc
     8101 tgccgcaagc actcaggg
//
