LOCUS       sequence-338787-       21241 bp    DNA     circular SYN 15-APR-2025
DEFINITION  CRISPR dCas9 directly fused to a catalytic inactive version of 
            bacterial DNA methyltransferase MQ1 to target to the FWA locus with 
            three guide RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21241)
  AUTHORS   Ghoshal B, Picard CL, Vong B, Feng S, Jacobsen SE
  TITLE     CRISPR-based targeting of DNA methylation in Arabidopsis thaliana by
            a bacterial CG-specific DNA methyltransferase.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jun 8;118(23):e2125016118. doi: 
            10.1073/pnas.2125016118.
  PUBMED    34074795
REFERENCE   2  (bases 1 to 21241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-06-8"; volume: "118(23):e2125016118. 
            doi"; pages: " 10.1073/pnas.2125016118"
COMMENT     Sequence Label: pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18
FEATURES             Location/Qualifiers
     source          1..21241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             425..1219
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2644..3232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3133..3152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3386..3403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3418..3558
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3484..3503)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3644..3666
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3902..4096
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4162..5235)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5664..6293)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    7594..7618
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     7806..7828
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7820..7837
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7821..7837
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7861..7879
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7861..7878
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      complement(7912..8619)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(9814..9834)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(9847..11361,11362..11364,11365..11430,
                     11431..11436,11437..13920,13921..13923,13924..13950))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(9847..11430,11431..11433,11434..13920,
                     13921..13923,13924..13950))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(9847..13950)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(13960..13980)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(13987..14052)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    14066..14121
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    complement(14140..14164)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             16875..17036
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             17064..17114
                     /codon_start=1
                     /product="nuclear localization signal from the human T-cell
                     leukemia virus type 1 (HTLV-1) Rex protein"
                     /label=Rex NLS
                     /translation="PKTRRRPRRSQRKRPPT"
     misc_RNA        17865..17940
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        18427..18502
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        18984..19059
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        19144..19820
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     19757..19777
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             19887..20912
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    20953..21127
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    21217..21241
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
       61 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
      121 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
      181 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
      241 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
      301 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
      361 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
      421 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
      481 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
      541 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
      601 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
      661 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
      721 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
      781 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
      841 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
      901 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
      961 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     1021 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     1081 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     1141 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     1201 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gctgagagat
     1261 cccctcataa tttccccaaa gcgtaaccat gtgtgaataa attttgagct agtagggttg
     1321 cagccacgag taagtcttcc cttgttattg tgtagccaga atgccgcaaa acttccatgc
     1381 ctaagcgaac tgttgagagt acgtttcgat ttctgactgt gttagcctgg aagtgcttgt
     1441 cccaaccttg tttctgagca tgaacgcccg caagccaaca tgttagttga agcatcaggg
     1501 cgattagcag catgatatca aaacgctctg agctgctcgt tcggctatgg cgtaggccta
     1561 gtccgtaggc aggacttttc aagtctcgga aggtttcttc aatctgcatt cgcttcgaat
     1621 agatattaac aagttgtttg ggtgttcgaa tttcaacagg taagttagtt gctagaatcc
     1681 atggctcctt tgccgacgct gagtagattt taggtgacgg gtggtgacaa tgagtccgtg
     1741 tcgagcgctg attttttcgg cctttagagc gagatttata caatagaatt tggcatgaga
     1801 ttggattgct tttagtcagc ctcttatagc ctaaagtctt tgagtgacta gatgacatat
     1861 catgtaagtt gctgataggt ttccagtttt ccgctcctag gtctgcatat tgtacttttc
     1921 ctcttactcg acttaaccag taccaaccca gcttctcaac ggatttatac catggcactt
     1981 taaagccagc atcactgaca atgagcggtg tggtgttact cggtagaatg ctcgcaaggt
     2041 cggctagaaa ttggtcatga gctttctttg aacattgctc tgaaagcggg aacgctttct
     2101 cataaagagt aacagaacga ccgtgtagtg cgactgaagc tcgcaatacc ataagccgtt
     2161 tttgctcacg gatatcagac cagtcaacaa gtacaatggg catcgtattg cccgaacaga
     2221 taaagctagc atgccaacgg tatacagcga gtcgctcttt gtggaggtga cgattaccta
     2281 acaatcggtc gattcgtttg atgttatgtt ttgttctcgc tttggttggc aggttacggc
     2341 caagttcggt aagagtgaga gttttacagt caagtaaggc gtggcaagcc aacgttaagc
     2401 tgttgagtcg ttttaagtgt aattcggggc agaattggta aagagagtcg tgtaaaatat
     2461 cgagttcgca cattttgttg tctgattatt gatttttggc gaaaccattt gatcatatga
     2521 caagatgtgt atctacctta acttaatgat tttgataaaa atcattaggg gattcatcag
     2581 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2641 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2701 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2761 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     2821 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2881 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2941 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3001 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3061 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     3421 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     3481 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     3541 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     3601 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     3661 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
     3721 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc
     3781 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
     3841 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
     3901 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
     3961 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
     4021 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
     4081 gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg
     4141 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
     4201 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
     4261 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
     4321 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
     4381 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
     4441 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
     4501 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
     4561 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tctttgtacg
     4621 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
     4681 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
     4741 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
     4801 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
     4861 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
     4921 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
     4981 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
     5041 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
     5101 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
     5161 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
     5221 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     5281 caggcaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     5341 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     5401 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     5461 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     5521 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     5581 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     5641 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     5701 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
     5761 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
     5821 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
     5881 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
     5941 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
     6001 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
     6061 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
     6121 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
     6181 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
     6241 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
     6301 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
     6361 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
     6421 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
     6481 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
     6541 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
     6601 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
     6661 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
     6721 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
     6781 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
     6841 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
     6901 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
     6961 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
     7021 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
     7081 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
     7141 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
     7201 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
     7261 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
     7321 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
     7381 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
     7441 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
     7501 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
     7561 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
     7621 cactgatagt ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta
     7681 gcattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     7741 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     7801 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcagag
     7861 atttaggtga cactatagaa tatcactagt aagctagctt gcatgccggt cctgctgagc
     7921 ctcgacatgt tgtcgcaaaa ttcgccctgg acccgcccaa cgatttgtcg tcactgtcaa
     7981 ggtttgacct gcacttcatt tggggcccac atacaccaaa aaaatgctgc ataattctcg
     8041 gggcagcaag tcggttaccc ggccgccgtg ctggaccggg ttgaatggtg cccgtaactt
     8101 tcggtagagc ggacggccaa tactcaactt caaggaatct cacccatgcg cgccggcggg
     8161 gaaccggagt tcccttcagt gagcgttatt agttcgccgc tcggtgtgtc gtagatacta
     8221 gcccctgggg cacttttgaa atttgaataa gatttatgta atcagtcttt taggtttgac
     8281 cggttctgcc gcttttttta aaattggatt tgtaataata aaacgcaatt gtttgttatt
     8341 gtggcgctct atcatagatg tcgctataaa cctattcagc acaatatatt gttttcattt
     8401 taatattgta catataagta gtagggtaca atcagtaaat tgaacggaga atattattca
     8461 taaaaatacg atagtaacgg gtgatatatt cattagaatg aaccgaaacc ggcggtaagg
     8521 atctgagcta cacatgctca ggttttttac aacgtgcaca acagaattga aagcaaatat
     8581 catgcgatca taggcgtctc gcatatctca ttaaagcagg actctagtta tcatggcgcg
     8641 ctagctaggg ccgccgatct tgtcaatgat ggcctccagc acctccacgc tgatgctgtt
     8701 gccacacacg aagatcttct ggttctcggt caggaactcg atttcgttca ctctcttgcc
     8761 gtcctggctg tcaaagccga tgtacaggaa ggtctcgtcg ctgttcatct tcctgatgtt
     8821 gctgccgtcc ttgatcttga tcctggagtt ggcgccggca gctgtcagtg tggggcctgt
     8881 gaactcgggg tcgtacacat agccctcgga gttgaacttg gagtagccga tcaggctggc
     8941 cttgttgatg ttggacttgg tcttcttgaa ttcggtgagg ttgtatttca gcaggttgtt
     9001 gaggatgtcc ttctcgctca cgatcttgtt cagcaccttt ttgatgctct tgggcttctt
     9061 gtcgccctta ggcagttcca cgaactcatt gagggtggag atcatgaaca ctctcctcct
     9121 ggcctggctg gatccgaaat cggcagcatt gaggacttcg atgctgttct ggtagcccag
     9181 gctctccagc ttctgcttcc attggttcag ctcctcctcg ttcttcttgt gcagcagggc
     9241 tcccacgttc tccatgagga ggtacttagg caggtcgttc ttctcggtgg agtccagggc
     9301 tctttcgatc tcccagagca ggccggatct ggtgccgctt cccctcttca tgcccttctg
     9361 gatgccttgc tggctcaggt cctgggaagg gaagctgtag gtgagcaggt cgatgttctt
     9421 cagtgtcctc ttgtagaggt ccctgatgtc gaaaatgttg ccctcctttt cggacagctt
     9481 gatggcgttg tagatgatct tcagctcgtc atccttcttc ctcttccagt atccgttgct
     9541 gacggggttc ttgctgttcc aggacagggt cttgttctcg aggtagtcga tcatctcctc
     9601 tctgctgacg gacttgtact cgagcttggt gtggaagttg ttatggatgg cctggtacat
     9661 gacgatggcg ggcacatacc actcggccag tcccacgatc tcgtactcgt ccttcctcac
     9721 tttctccagg gcctttctct gggcgccaat gccggcgaag gcttcgaaca ctctcagctt
     9781 ctttgttttg ttctccactt tgctgtccct agccaccttc ctcttcttct tggggtcagc
     9841 cctgctgtct ccaccgagct gagagaggtc gattcttgtt tcatagagcc ccgtaattga
     9901 ctgatgaatc agtgtggcgt ccaggacctc ctttgtagag gtgtaccgct ttctgtctat
     9961 ggtggtgtcg aagtacttga aggctgcagg cgcgcccaag ttggtcagag taaacaagtg
    10021 gataatgttt tctgcctgct ccctgatggg cttatccctg tgcttattgt aagcagaaag
    10081 caccttatcg aggttagcgt cggcgaggat cactcttttg gagaattcgc ttatttgctc
    10141 gatgatctca tcaaggtagt gtttgtgttg ttccacgaac agctgcttct gctcattatc
    10201 ttcgggagac cctttgagct tttcatagtg gctggccaga tacaagaaat taacgtattt
    10261 agagggcagt gccagctcgt tacctttctg cagctcgccc gcactagcga gcattcgttt
    10321 ccggccgttt tcaagctcaa agagagagta cttgggaagc ttaatgatga ggtctttttt
    10381 gacctcttta tatcctttcg cctcgagaaa gtcgatgggg tttttttcga agcttgatcg
    10441 ctccatgatt gtgatgccca gcagttcctt gacgcttttg agttttttag acttcccttt
    10501 ctccactttg gccacaacca gtacactgta agcgactgta ggagaatcga atccgccgta
    10561 tttcttgggg tcccaatctt ttttgcgtgc gatcagcttg tcgctgttcc ttttcgggag
    10621 gatactttcc ttggagaagc ctccggtctg tacttcggtc tttttaacga tgttcacctg
    10681 cggcatggac aggaccttcc ggactgtcgc gaaatcccta cccttgtccc acacgatttc
    10741 tcctgtttct ccgtttgttt cgataagtgg tcgcttccga atctctccat tggccagtgt
    10801 aatctcggtc ttgaaaaaat tcataatatt gctgtaaaag aagtacttag cggtggcctt
    10861 gcctatttcc tgctcagact ttgcgatcat tttcctaaca tcgtacactt tatagtctcc
    10921 gtaaacaaat tcagattcaa gcttgggata ttttttgata agtgcagtgc ctaccactgc
    10981 attcaggtag gcatcatgcg catggtggta attgttgatc tctctcacct tataaaactg
    11041 aaagtccttt ctgaaatctg agaccagctt agacttcaga gtaataactt tcacctctcg
    11101 aatcagtttg tcattttcat cgtacttggt gttcatgcgt gaatcgagaa tttgggccac
    11161 gtgcttggtg atctggcgtg tctcaacaag ctgccttttg atgaagccgg ctttatccaa
    11221 ctcagacagg ccacctcgtt cagccttagt cagattatcg aacttccgtt gtgtgatcag
    11281 tttggcgttc agcagctgcc gccaataatt tttcattttc ttgacaactt cttctgaggg
    11341 gacgttatca ctcttccctc tatttttatc ggatcttgtc aacactttat tatcaataga
    11401 atcatctttg agaaaagact ggggcacgat ggcatccacg tcgtagtcgg agagccgatt
    11461 gatgtccagt tcctgatcca cgtacatgtc cctgccgttc tgcaggtagt acaggtagag
    11521 cttctcattc tgaagctggg tgttttcaac tgggtgttcc ttaaggattt gggaccccag
    11581 ttcttttata ccctcttcaa tcctcttcat cctttcccta ctgttcttct gtcccttctg
    11641 ggtagtttgg ttctctcggg ccatctcgat aacgatattc tcgggcttat gccttcccat
    11701 tactttgacg agttcatcca cgaccttaac ggtctgcagt attccctttt tgatagctgg
    11761 gctacctgca agattagcga tgtgctcgtg aagactgtcc ccctggccag aaacttgtgc
    11821 tttctggatg tcctccttaa aggtgagaga gtcatcatgg atcaactgca tgaagttccg
    11881 gttggcaaat ccatcggact taagaaaatc caggattgtc tttccactct gcttgtctcg
    11941 gatcccattg atcagttttc ttgacagccg cccccatcct gtatatcggc gcctcttgag
    12001 ctgtttcatg actttgtcgt cgaagagatg agcgtaagtt ttcaagcgtt cttcaatcat
    12061 ctccctatct tcaaacaacg taagggtgag gacaatgtcc tcaagaatgt cctcgttctc
    12121 ctcattgtcc aggaagtcct tgtctttaat gattttcagg agatcgtgat acgttcccag
    12181 ggatgcgttg aagcgatcct ccactccgct gatttcaaca gagtcgaaac attcaatctt
    12241 tttgaaatag tcttctttga gctgtttcac ggtaactttc cggttcgtct tgaagaggag
    12301 gtccacgata gctttcttct gctctccaga caggaatgct ggctttctca tcccttctgt
    12361 gacgtatttg accttggtga gctcgttata aactgtgaag tactcgtaca gcagagagtg
    12421 tttaggaagc accttttcgt taggcagatt tttatcaaag ttagtcatcc tttcgatgaa
    12481 ggactgggca gaggccccct tatccacgac ttcctcgaag ttccagggag tgatggtctc
    12541 ttctgatttg cgagtcatcc acgcgaatct ggaatttccc cgggcgaggg ggcctacata
    12601 gtagggtatc cgaaatgtga ggattttctc aatcttttcc ctgttatctt tcaaaaaggg
    12661 gtagaaatcc tcttgccgcc tgaggatagc gtgcagttcg cccaggtgaa tctggtgggg
    12721 gatgcttcca ttgtcgaaag tgcgctgttt gcgcaacaga tcttctctgt taagctttac
    12781 cagcagctcc tcggtgccgt ccattttttc caagatgggc ttaataaatt tgtaaaattc
    12841 ctcctggctt gctccgccgt caatgtatcc ggcgtagcca tttttagact gatcgaagaa
    12901 aatttccttg tacttctcag gcagttgctg tctgacaagg gccttcagca aagtcaagtc
    12961 ttggtggtgc tcatcatagc gcttgatcat actagcgctc agcggagctt tggtgatctc
    13021 cgtgttcact cgcagaatat cactcagcag aatggcgtct gacaggttct ttgccgccaa
    13081 aaaaaggtct gcgtactggt cgccgatctg ggccagcaga ttgtcgagat catcatcgta
    13141 ggtgtctttg ctcagttgaa gcttggcatc ttcggccagg tcgaagttag atttaaagtt
    13201 gggggtcagc ccgagtgaca gggcgataag attaccaaac aggccgttct tcttctcccc
    13261 agggagctgt gcgatgaggt tttcgagccg ccgggatttg gacagcctag cgctcaggat
    13321 tgctttggcg tcaactccgg atgcgttgat cgggttctct tcgaaaagct gattgtaagt
    13381 ctgaaccagt tggataaaga gtttgtcgac atcgctgttg tctgggttca ggtccccctc
    13441 gatgaggaag tgtccccgaa atttgatcat atgcgccagc gcgagataga tcaaccgcaa
    13501 gtcagcctta tcagtactgt ctacaagctt cttcctcaga tgatatatgg ttgggtactt
    13561 ttcatggtac gccacctcgt ccacgatatt gccaaagatt gggtggcgct cgtgcttttt
    13621 atcctcctcc accaaaaagg actcctccag cctatggaag aaagagtcat ccaccttagc
    13681 catctcatta ctaaagatct cctgcaggta gcagatccga ttctttctgc gggtatatct
    13741 gcgccgtgct gttcttttga gccgcgtggc ttcggcggtt tccccggagt cgaacaggag
    13801 ggcgccaatg aggttcttct ttatgctgtg gcgatcggta ttgcccagaa ctttgaattt
    13861 tttgctcggc accttgtact cgtccgtaat gacggcccag ccgacgctgt ttgtgccgat
    13921 ggcgagccca atggagtact tcttgtccat tccgcggccc accttcctct tcttcttggg
    13981 ggccatcttg tcatcgtcat ccttgtaatc gatgtcatga tctttataat caccgtcatg
    14041 gtctttgtag tccataactt tttttgtaat tgtaaatagt aattgtaatg ttgtttgttg
    14101 tttgttgttg ttggtaattg ttgtaaaaat actttaaaga gcctgctttt ttgtacaaac
    14161 ttgttgatga tccctgttaa tcagaaaaac tcagattaat cgacaaattc gatcgcacaa
    14221 actagaaact aacaccagat ctagatagaa atcacaaatc gaagagtaat tattcgacaa
    14281 aactcaaatt atttgaacaa atcggatgat atctatgaaa ccctaatcga gaattaagat
    14341 gatatctaac gatcaaaccc agaaaatcgt cttcgatcta agattaacag aatctaaacc
    14401 aaagaacata tacgaaattg ggatcgaacg aaaacaaaat cgaagatttt gagagaataa
    14461 ggaacacaga aatttacctt gatcacggta gagagaattg agagaaagtt tttaagattt
    14521 tgagaaattg aaatctgaat tgtgaagaag aagagctctt tgggtattgt tttatagaag
    14581 aagaagaaga aaagacgagg acgactaggt cacgagaaag ctaaggcggt gaagcaatag
    14641 ctaataataa aatgacacgt gtattgagcg ttgtttacac gcaaagttgt ttttggctaa
    14701 ttgccttatt tttaggttga ggaaaagtat ttgtgctttg agttgataaa cacgactcgt
    14761 gtgtgccggc tgcaaccact ttgacgccgt ttattactga ctcgtcgaca accacaattt
    14821 ctaacggtcg tcataagatc cagccgttga gatttaacga tcgttacgat ttatattttt
    14881 ttagcattat cgttttattt tttaaatata cggtggagct gaaaattggc aataattgaa
    14941 ccgtgggtcc cactgcattg aagcgtattt cgtattttct agaattcttc gtgctttatt
    15001 tcttttcctt tttgtttttt tttgccattt atctaatgca agtgggctta taaaatcagt
    15061 gaatttcttg gaaaagtaac ttctttatcg tataacatat tgtgaaatta tccatttctt
    15121 ttaatttttt agtgttattg gatatttttg tatgattatt gatttgcata ggataatgac
    15181 ttttgtatca agttggtgaa caagtctcgt taaaaaaggc aagtggtttg gtgactcgat
    15241 ttattcttgt tatttaattc atatatcaat ggatcttatt tggggcctgg tccatattta
    15301 acactcgtgt tcagtccaat gaccaataat attttttcat taataacaat gtaacaagaa
    15361 tgatacacaa aacattcttt gaataagttc gctatgaaga agggaactta tccggtccta
    15421 gatcatcagt tcatacaaac ctccatagag ttcaacatct taaacaagaa tatcctgatc
    15481 tgaagaatgt ggaggcttta gtcccttgga tacttgggag gctgtggaag aacagaaacg
    15541 agctggtgct taaagggagg gaatttggaa ccaatgaggt attagtaagg acacaagaag
    15601 atgcagatga gtggattaga aggaaagagg ctcagaatgt aaggaaagca ccaaccacaa
    15661 caccgtcggg acagacgaga aacatgggaa gctccaccac aatcttgggt taagtgcaac
    15721 tttgatgggg catggccaac agaaggatta aaatgtggct tagggtgggt gcttcgcgat
    15781 catacaggga aggtgttatg gttaggtgca agagctgtgg taaaagtaag aagcgtgctg
    15841 gaagtagaag tggaggctct tagatgggct gtgctgtcat tatcccgatt caattatagg
    15901 aagatcattt ttgaggtgga ttctcagcaa cttgtgtctt tggttacatg aaagttatgc
    15961 ttgtcaagtc tcaatccaat tatccaagac ataaagtatc tacttagcaa gtttgaggat
    16021 tttatgcttg tgcatacaag ccgagaagga aatggagtgg cagatagaat agctaaggaa
    16081 tctctttctt ttgagaatta tgatctaaag ttgtattcta ttgtaccaat ttgggttaaa
    16141 agctctgttg agctagactc atatgcatgc ggccgcgttc ctaacacctg gagaaccttt
    16201 tatgtacttc acaaccctca atgctgcttc caggtgtgac tgtttgggtt gctgcatgaa
    16261 ttgactcagc acctgcactg caaagctgat atctggcctt gtgattgtca agtagagaag
    16321 ctttcccacc agtctctgat aggaacccac atcctctaac tctgcatcat cagtctttcc
    16381 taagtgcttg tcatattcta cagtagtcag cctttggttc tgttccattg gtgttgacac
    16441 tggtctgcag cctcccagac caacacctga tatcagttcc agtgcatact tcctctggtt
    16501 cagtaggatt cccttttctg atctcagcac ctcaatgcct aagaagtatt ttagttctcc
    16561 caaatctttc atcttgaaat gctgatgcag ggttgccttt gcttctgaaa tcaaaacatt
    16621 gctgctgcct gttattaaca gatcatccac ataaatcagg attatgacaa ggtcagtccc
    16681 ctctcttttg gtaaacaagg agtgatcata agcactttgc ataaaaccag cctgcataag
    16741 gacagtggta agcttgatgt tccactgcct tgatgcttgc tttaaaccat agagggattc
    16801 aacagcctgc acactttggt ctccccttgg ctgtgaaaac cctgagtacg ggaggaggat
    16861 ctcggaccga agagtacaag cttatcctga acggtaaaac cctgaaaggt gaaaccacca
    16921 ccgaagctgt tgacgctgct accgcggaaa aagttttcaa acagtacgct aacgacaacg
    16981 gtgttgacgg tgaatggacc tacgacgacg ctaccaaaac cttcacggta accgaaggtg
    17041 gtggtagcgg tggtggtact agtccaaaaa caaggaggag accgcgaaga tcacaacgga
    17101 aaaggccgcc tacgccatgg ccgtaagttt gagctcgaat ttccccgatc gttcaaacat
    17161 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
    17221 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
    17281 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
    17341 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tacgaagctt
    17401 tcgttgaaca acggaaactc gacttgcctt ccgcacaata catcatttct tcttagcttt
    17461 ttttcttctt cttcgttcat acagtttttt tttgtttatc agcttacatt ttcttgaacc
    17521 gtagctttcg ttttcttctt tttaactttc cattcggagt ttttgtatct tgtttcatag
    17581 tttgtcccag gattagaatg attaggcatc gaaccttcaa gaatttgatt gaataaaaca
    17641 tcttcattct taagatatga agataatctt caaaaggccc ctgggaatct gaaagaagag
    17701 aagcaggccc atttatatgg gaaagaacaa tagtatttct tatataggcc catttaagtt
    17761 gaaaacaatc ttcaaaagtc ccacatcgct tagataagaa aacgaagctg agtttatata
    17821 cagctagagt cgaagtagtg attgacggaa agatgtatgg gcttgtttta gagctagaaa
    17881 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
    17941 tttttttggt ttaggaaagc tttcgttgaa caacggaaac tcgacttgcc ttccgcacaa
    18001 tacatcattt cttcttagct ttttttcttc ttcttcgttc atacagtttt tttttgttta
    18061 tcagcttaca ttttcttgaa ccgtagcttt cgttttcttc tttttaactt tccattcgga
    18121 gtttttgtat cttgtttcat agtttgtccc aggattagaa tgattaggca tcgaaccttc
    18181 aagaatttga ttgaataaaa catcttcatt cttaagatat gaagataatc ttcaaaaggc
    18241 ccctgggaat ctgaaagaag agaagcaggc ccatttatat gggaaagaac aatagtattt
    18301 cttatatagg cccatttaag ttgaaaacaa tcttcaaaag tcccacatcg cttagataag
    18361 aaaacgaagc tgagtttata tacagctaga gtcgaagtag tgattggcgc tcgtatgaat
    18421 gttgaagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
    18481 aaaagtggca ccgagtcggt gcttttttta aacaagcttt cgttgaacaa cggaaactcg
    18541 acttgccttc cgcacaatac atcatttctt cttagctttt tttcttcttc ttcgttcata
    18601 cagttttttt ttgtttatca gcttacattt tcttgaaccg tagctttcgt tttcttcttt
    18661 ttaactttcc attcggagtt tttgtatctt gtttcatagt ttgtcccagg attagaatga
    18721 ttaggcatcg aaccttcaag aatttgattg aataaaacat cttcattctt aagatatgaa
    18781 gataatcttc aaaaggcccc tgggaatctg aaagaagaga agcaggccca tttatatggg
    18841 aaagaacaat agtatttctt atataggccc atttaagttg aaaacaatct tcaaaagtcc
    18901 cacatcgctt agataagaaa acgaagctga gtttatatac agctagagtc gaagtagtga
    18961 ttgatgttga atgggataaa gaggttttag agctagaaat agcaagttaa aataaggcta
    19021 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggta ccatggtgga
    19081 gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc
    19141 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
    19201 tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca
    19261 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg
    19321 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
    19381 agtggattga tgtgaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
    19441 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa
    19501 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg
    19561 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct
    19621 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
    19681 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg
    19741 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
    19801 atttggagag gacacgctga aatcaccagt ctctctctac aaatctatct ctctcgagct
    19861 ttcgcagatc cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg
    19921 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
    19981 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
    20041 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
    20101 tcccgattcc ggaagtgctt gacattgggg agtttagcga gagcctgacc tattgcatct
    20161 cccgccgttc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
    20221 tacaaccggt cgcggaggct atggatgcga tcgctgcggc cgatcttagc cagacgagcg
    20281 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
    20341 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
    20401 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
    20461 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
    20521 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
    20581 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
    20641 ggcatccgga gcttgcagga tcgccacgac tccgggcgta tatgctccgc attggtcttg
    20701 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
    20761 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
    20821 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
    20881 gccccagcac tcgtccgagg gcaaagaaat agagtagatg ccgaccggga tctgtcgatc
    20941 gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg gaattagggt
    21001 tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
    21061 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat
    21121 ccagatcccc cgaattagag ctcgaattaa ttcggcgtta attcagtaca ttaaaaacgt
    21181 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
    21241 a
//
