LOCUS       sequence-339831-       10626 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses uTEV1 and reporter receiver construct in DIPG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Cho KF, Gillespie SM, Kalogriopoulos NA, Quezada MA, Jacko M, Monje 
            M, Ting AY
  TITLE     A light-gated transcriptional recorder for detecting cell-cell 
            contacts.
  JOURNAL   Elife. 2022 Mar 21;11:e70881. doi: 10.7554/eLife.70881.
  PUBMED    35311648
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-03-21"; volume: "11:e70881. doi"; pages: " 
            10.7554/eLife.70881"
COMMENT     Sequence Label: pPB UbC Arrestin-uTEV1-V5; UAS-mCherry
FEATURES             Location/Qualifiers
     source          1..10626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    1367..1484
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     5'UTR           1988..2030
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      2076..2085
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2082..2792
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2227..2245)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2495..2514
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2808..3396
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3279..3290)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3403..4614
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4451..4470
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4631..4640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6554..6595
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6605..6661
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      6668..6677
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6674..7072
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7196..7317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7233..7252)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7287..7306
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8080..8114
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8605..8621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8605..8621)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8618..8640)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8629..8645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8653..8659,8660..8677,8678..8683))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8698..8719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8836..8853)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9007..9595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9087..9106)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9766..10557,10558..10626))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10389..10408
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagac tagaactttt aaaagaaaag
     1381 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     1441 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttatcgat cacgagacta
     1501 gcctcgaggg atcgcaaaaa gcttaggcct ccaaggcgga gtactgtcct ccgggctggc
     1561 ggagtactgt cctccggcaa ggtcggagta ctgtcctccg acactagagg tcggagtact
     1621 gtcctccgac gcaaggcgga gtactgtcct ccgggctgcg gagtactgtc ctccggcaag
     1681 gtcggagtac tgtcctccga cactagaggt cggagtactg tcctccgacg caaggtcgga
     1741 gtactgtcct ccgacactag aggtcggagt actgtcctcc gacgcaaggt cggagtactg
     1801 tcctccgaca ctagaggtcg gagtactgtc ctccgacgca aggcggagta ctgtcctccg
     1861 ggctggcgga gtactgtcct ccggcaaggg tcgactctag agggtatata atggatccca
     1921 tcgcgtctca gcctcacttt gagctcctcc acacgaattc cccgataccg tcgattcaag
     1981 gagcttgctt gttctttttg cagaagctca gaataaacgc tcaactttgg cagatctacc
     2041 aaggagctcg gatccactag tgatcccctg ctagcgccac catggtgagc aagggcgagg
     2101 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     2161 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     2221 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     2281 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     2341 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     2401 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     2461 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     2521 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     2581 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     2641 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     2701 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     2761 ccaccggcgg catggacgag ctgtacaagt gaagcggccg cgtcgacaat caacctctgg
     2821 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2881 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2941 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     3001 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3061 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     3121 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3181 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     3241 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3301 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3361 agacgagtcg gatctccctt tgggccgcct ccccgcggat ccggcctccg cgccgggttt
     3421 tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc
     3481 gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag
     3541 actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact
     3601 ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg
     3661 gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc
     3721 cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg
     3781 atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc
     3841 cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg
     3901 tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt
     3961 tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg
     4021 gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct
     4081 cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact
     4141 gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg
     4201 cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg
     4261 ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt
     4321 cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc
     4381 tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt
     4441 cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt
     4501 gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt
     4561 tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacgctagc
     4621 ggattctaga gccaccatgg gtgaaaaacc cgggaccagg gtcttcaaga agtcgagccc
     4681 taactgcaag ctcaccgtgt acttgggcaa gcgtgacttt gtggatcact tggacaaagt
     4741 ggatcctgtc gatggtgtgg tgcttgtgga tcctgactac ttgaaggacc ggaaagtgtt
     4801 tgtgaccctc acctgtgcct tccgctatgg ccgagaagac ctggatgtac tgggcctgtc
     4861 tttccgcaaa gatctgttca tcgccaccta ccaggccttc ccccccatgc ccaacccacc
     4921 tcggcccccc acccgcctac aggaccgact gctgaagaag ttgggccagc acgcccaccc
     4981 cttttttttc acaatacccc agaatttgcc ttgctccgtc acactgcagc caggaccgga
     5041 ggacacaggg aaggcctgtg gagtagactt tgagattcga gccttctgtg ccaaatctat
     5101 agaagaaaaa agccacaaaa ggaactccgt gcggcttatc atcagaaagg tacagtttgc
     5161 tcctgagaca cccggccccc agccatcagc tgaaaccaca cgccacttcc tcatgtctga
     5221 ccggaggtcc ctgcacctag aggcttccct ggacaaagag ctgtactacc atggggaacc
     5281 cctcaatgtc aacgtccacg tcaccaacaa ttctgccaaa accgtcaaga agatcagagt
     5341 gtctgtgaga cagtatgccg acatttgcct cttcagcacc gcgcagtaca agtgtcctgt
     5401 ggctcagctt gaacaagatg accaggtgtc tcccagttcc acattctgca aggtgtacac
     5461 cataaccccg ctgctcagtg acaaccgaga gaagcgtggc cttgcccttg atgggcaact
     5521 caagcacgaa gacaccaacc tggcttccag caccattgtg aaggagggag ccaacaagga
     5581 ggtgctggga atcctagtat cctacagggt caaggtgaag ctggtggtgt ctcgaggcgg
     5641 ggatgtctcc gtggagctac ctttcgtcct aatgcacccc aagccccacg accacatcac
     5701 ccttccccga ccccagtcag ccccccggga aatagacatc cctgtggata ccaacctcat
     5761 tgaattcgat accaactatg ccacagacga cgacatcgtg tttgaggact ttgcgaggct
     5821 tcggctgaag gggatgaagg atgacgactg tgatgaccag ttctgcggtg gaagtggatc
     5881 aggcagcggt ggatctggag aaagcttgtt taagggacca cgtgattaca acccgatatc
     5941 gagcaccatt tgtcatttga cgaatgaatc tgatgggcac acaacatcgt tgtatggtat
     6001 tggatttggt cccttcatca ttacaaacaa gcacttgttt agaagaaata atggaacact
     6061 gttggtccaa tcactacatg gtgtattcaa ggtcaagaac accacgactt tgcaacaaca
     6121 cctcattgat gggagggaca tgataattat tcgcatgcct aaggatttcc caccatttcc
     6181 tcaaaagctg aaatttagag agccacaaag ggaagagcgc atatgtcttg tgacaaccaa
     6241 cttccaaact aagagcatgt ctagcatggt gtcagacact agttgcacat tcccttcatc
     6301 tgatggcata ttctggaagc attggattca aaccaaggat gggcagtgtg gcaatccatt
     6361 agtatcaact agagatgggt tcattgttgg tatacactca gcatcgaatt tcaccaacac
     6421 aaacaattat ttcacaagcg tgccgaaaaa cttcatggaa ttgttgacaa atcaggaggc
     6481 gcagcagtgg gttagtggtt ggcgattaaa tgctgactca gtattgtggg ggggccataa
     6541 agttttcatg gtgggcaagc cgatccctaa ccctctgctg ggcctggaca gcactggaag
     6601 cggagctact aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc
     6661 ttctagagcc accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     6721 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     6781 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     6841 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     6901 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     6961 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     7021 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct aagtcgacaa
     7081 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7141 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgttaact aactaaactt
     7201 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7261 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7321 tgtctggaat tgactcaaat gatgtcaatt agtctatcag aagctcatct ggtctccctt
     7381 ccgggggaca agacatccct gtttaatatt taaacagcag tgttcccaaa ctgggttctt
     7441 atatcccttg ctctggtcaa ccaggttgca gggtttcctg tcctcacagg aacgaagtcc
     7501 ctaaagaaac agtggcagcc aggtttagcc ccggaattga ctggattcct tttttagggc
     7561 ccattggtat ggctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     7621 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     7681 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc
     7741 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg
     7801 tccctgatat ctataacaag aaaatatata tataataagt tatcacgtaa gtagaacatg
     7861 aaataacaat ataattatcg tatgagttaa atcttaaaag tcacgtaaaa gataatcatg
     7921 cgtcattttg actcacgcgg tcgttatagt tcaaaatcag tgacacttac cgcattgaca
     7981 agcacgcctc acgggagctc caagcggcga ctgagatgtc ctaaatgcac agcgacggat
     8041 tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga
     8101 ctatctttct agggttaatc tagctgcatc aggatcatat cgtcgggtct tttttccggc
     8161 tcagtcatcg cccaagctgg cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt
     8221 cccgcgaggt tgaagcggca tggaaagagt ttgccgagga tgactgctgc tgcattgacg
     8281 ttgagcgaaa acgcacgttt accatgatga ttcgggaagg tgtggccatg cacgccttta
     8341 acggtgaact gttcgttcag gccacctggg ataccagttc gtcgcggctt ttccggacac
     8401 agttccggat ggtcagcccg aagcgcatca gcaacccgaa caataccggc gacagccgga
     8461 actgccgtgc cggtgtgcag attaatgaca gcggtgcggc gctgggatat tacgtcagcg
     8521 aggacgggta tcctggctgg atgccgcaga aatggacatg gataccccgt gagttacccg
     8581 gcgggcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8641 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8701 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8761 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     8821 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8881 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8941 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9001 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9061 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9121 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9181 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9241 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9301 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9361 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9421 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9481 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9541 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9601 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9661 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9721 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9781 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9841 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9901 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9961 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10021 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10081 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10141 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10201 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10261 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10321 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10381 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10441 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10501 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10561 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10621 actcat
//
