LOCUS       sequence-340224-        8901 bp    DNA     circular SYN 14-APR-2025
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: MAC-TOP3B
FEATURES             Location/Qualifiers
     source          1..8901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(959..1750,1751..1819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1582..1601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1820..1924)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1999..2018)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2190..2569
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2570..2773
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2724..2744
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2775..2793
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2796..2814
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2824..2848
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3016..3366,3367..3369,3370..3978)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             3979..4005
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4036..4059
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4096..4119
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    4132..4156
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4578..4587
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6746..6770)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6808..6825)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6814..7038
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7171..7190)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7322..7369
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7377..8399
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7377..8399
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8529..8650
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8566..8585)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8620..8639
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8699..8715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8699..8715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8712..8734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8723..8739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8747..8753,8754..8771,8772..8777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8792..8813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1921 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     1981 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     2041 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     2101 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     2161 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     2221 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2281 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2341 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2401 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2461 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2521 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2581 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2641 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2701 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2761 tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg
     2821 acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2881 ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg
     2941 taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatcc agcacagtgg
     3001 cggccgctcg agaccatgaa ggacaacacc gtgcccctga agctgatcgc cctgctggcc
     3061 aacggcgagt tccactctgg cgagcagctg ggcgagacac tgggcatgag cagagccgcc
     3121 atcaacaagc acatccagac cctgcgggac tggggcgtgg acgtgttcac agtgcccggc
     3181 aagggctaca gcctgcccga gcccatccag ctgctgaacg ccaagcagat cctgggccag
     3241 ctggatggcg gatctgtggc cgtgctgccc gtgatcgaca gcaccaacca gtatctgctc
     3301 gaccggatcg gcgagctgaa gtccggcgac gcctgtatcg ccgagtacca gcaggccggc
     3361 agaggcggca ggggcagaaa gtggttcagc cccttcggcg ccaacctgta cctgagcatg
     3421 ttctggagac tggaacaggg cccagccgcc gctattggcc tgagcctggt catcggcatc
     3481 gtgatggccg aggtgctgcg gaagctgggc gccgacaaag tgcgcgtgaa gtggcccaac
     3541 gacctgtatc tgcaggaccg gaagctggcc ggcatcctgg tggaactgac cggcaaaaca
     3601 ggcgacgccg cccagatcgt gatcggcgct ggcatcaaca tggccatgcg gcgggtggaa
     3661 gagagcgtgg tcaaccaggg ctggatcacc ctgcaggaag ccggcatcaa cctggaccgg
     3721 aacaccctgg ccgccatgct gatcagagag ctgagagccg ccctggaact gttcgagcag
     3781 gaaggcctgg ccccctacct gagcagatgg gagaagctgg acaacttcat caaccggccc
     3841 gtgaaactga tcatcggcga caaagagatc ttcggcatca gccggggcat cgacaagcag
     3901 ggggccctgc tcctggaaca ggacggcatc atcaagccct ggatgggcgg cgagatcagc
     3961 ctgcggagcg ccgagaagta cccatacgat gttcctgact atgccggtac cgagctcgga
     4021 tccaccatgg ctagctggag ccacccgcag ttcgagaaag gtggaggttc cggaggtgga
     4081 tcgggaggtg gatcgtggag ccacccgcag ttcgaaaaag cggccgatat cacaagtttg
     4141 tacaaaaaag ttggcaccat gaagactgtg ctcatggttg ctgaaaagcc gtccttggca
     4201 cagtcaattg ccaaaatcct ctctagaggg agcctgtcct cacacaaagg gctgaacggg
     4261 gcctgctcag tccacgagta cactgggacc tttgctggcc agccagtgcg cttcaagatg
     4321 acgtctgtct gtggtcacgt gatgaccctg gatttcctgg gaaaatacaa caaatgggac
     4381 aaagtggacc ccgcagaact gttcagccaa gctcccacgg agaagaaaga agctaacccc
     4441 aagctgaaca tggtgaagtt cctgcaggtg gagggcagag gctgcgacta catcgtgctg
     4501 tggctggact gcgacaagga gggggagaac atctgctttg aggttcttga tgctgttctg
     4561 cccgtcatga acaaggccca tggtggcgag aagaccgtgt tccgggccag gtttagctcc
     4621 atcacggaca cagacatctg taatgccatg gcctgcctag gcgagcctga ccacaacgag
     4681 gcgctctcag tggatgctcg ccaggagctg gacctgcgaa tcggctgtgc attcaccagg
     4741 tttcagacta aatatttcca ggggaaatac ggtgatttag acagctctct catctccttt
     4801 gggccgtgtc agactccaac cctgggattc tgtgtggaga gacatgataa aatccagtcc
     4861 ttcaaaccag agacctactg ggtgctgcag gccaaggtta acactgacaa agacagatct
     4921 ctccttttgg actgggaccg agtaagagtg tttgaccggg agatcgcaca gatgttttta
     4981 aacatgacaa agctggagaa ggaagcccag gtggaggcca caagcaggaa agaaaaggcc
     5041 aagcagaggc ccctggccct gaacactgtg gagatgctgc gtgtggccag ctcttctctg
     5101 ggcatggggc cgcagcacgc catgcagacg gctgagcggc tctacacgca aggctacatc
     5161 agctacccac ggacagagac cacccactac cctgagaact ttgacctgaa gggctctctg
     5221 cggcagcagg ccaaccaccc ctactgggcc gacacggtga agcggttgtt agcagaaggt
     5281 atcaaccgcc cgcggaaagg ccatgacgcc ggcgaccatc cccccatcac ccccatgaag
     5341 tctgccacag aggccgaatt agggggtgac gcgtggcggc tctatgagta catcaccaga
     5401 cacttcatcg ccacggtcag ccatgactgc aagtacctgc agagcaccat ctccttcaga
     5461 attgggcccg agctcttcac ctgctccggg aagaccgtcc tctcaccagg cttcacggag
     5521 gtcatgccct ggcagagcgt gcccctggag gagagcctgc ccacttgcca gcggggtgat
     5581 gccttccctg tgggcgaggt gaagatgctg gagaagcaga cgaacccacc cgactacctg
     5641 acggaggccg agctcatcac gctcatggag aagcatggca tcggcacgga tgccagcatc
     5701 cctgtgcata tcaacaacat ctgccagcgc aactatgtca cggtggagag cgggcgccgg
     5761 ctcaagccca ccaacctcgg catcgtcctg gtgcacggct actataagat tgatgcagag
     5821 ctggtgctcc ccaccatccg cagtgcagtg gagaagcagc tgaacctgat cgcccagggc
     5881 aaggccgact accgccaggt cctgggccac accctggacg tgttcaagag gaagttccac
     5941 tactttgtcg actccattgc tggcatggat gagttgatgg aggtgtcttt ctcgcccctg
     6001 gcggccacag gcaagcccct ctcacgctgt gggaagtgcc accgcttcat gaagtacatc
     6061 caggccaagc caagccgcct gcactgctcc cactgcgatg agacctacac gctcccccag
     6121 aacggcacca tcaagctcta caaggagctc cgctgccctc tggatgactt cgagctggtc
     6181 ctgtggtcat caggctctcg gggcaagagc tacccgctgt gcccctactg ctacaaccac
     6241 ccacccttcc gagacatgaa gaaaggcatg ggctgcaacg agtgtacgca cccctcctgc
     6301 cagcactcgc tgagcatgct gggcatcggc cagtgcgtgg aatgtgagag cggggtgctg
     6361 gtgctggacc ccacctcggg ccccaagtgg aaggtggcct gcaacaagtg caacgtggta
     6421 gcgcactgct tcgagaacgc ccaccgcgtg cgggtgtccg ccgacacctg cagtgtctgt
     6481 gaggccgcct tgcttgatgt ggacttcaac aaggccaagt ccccactccc gggcgatgag
     6541 acgcagcaca tgggctgcgt cttttgtgac cccgtcttcc aggagctggt ggagctgaag
     6601 catgcggcct cctgccaccc catgcaccgc ggtggaccag ggagaaggca gggtcgaggg
     6661 cggggccggg ccaggaggcc ccctgggaag cccaacccca gacggcccaa ggacaagatg
     6721 tcagccctgg ccgcctactt tgtatgacca actttcttgt acaaagtggt gacgtaagct
     6781 aggggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     6841 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6901 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6961 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7021 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     7081 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7141 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7201 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7261 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtacct
     7321 agaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcc ttggccaaaa
     7381 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     7441 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     7501 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     7561 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     7621 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     7681 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     7741 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     7801 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     7861 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     7921 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     7981 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     8041 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     8101 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     8161 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     8221 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     8281 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     8341 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc
     8401 acgtactacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     8461 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     8521 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8581 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8641 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     8701 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8761 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8821 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8881 gccaacgcgc ggggagaggc g
//
