LOCUS       sequence-340273-        6007 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Localization: Microtubules, Excitation: 683, Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: mIFP-MAPTau-N-10
FEATURES             Location/Qualifiers
     source          1..6007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      559..568
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1651..2610
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     polyA_signal    2735..2856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2772..2791)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2826..2845
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2863..3318)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3000..3021)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3212..3231
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3345..3449
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3451..3808
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3451..3642
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3474..3494)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3659..3794
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3843..4637
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3843..4637
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3897..3916)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4507..4526
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4825..4844)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4869..4916
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5245..5833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5734..5753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgaggc caccatggct gagccccgcc aggagttcga agtgatggaa
      601 gatcacgctg ggacgtacgg gttgggggac aggaaagatc aggggggcta caccatgcac
      661 caagaccaag agggtgacac ggacgctggc ctgaaagctg aagaagcagg cattggagac
      721 acccccagcc tggaagacga agctgctggt cacgtgaccc aagctcgcat ggtcagtaaa
      781 agcaaagacg ggactggaag cgatgacaaa aaagccaagg gggctgatgg taaaacgaag
      841 atcgccacac cgcggggagc agcccctcca ggccagaagg gccaggccaa cgccaccagg
      901 attccagcaa aaaccccgcc cgctccaaag acaccaccca gctctggtga acctccaaaa
      961 tcaggggatc gcagcggcta cagcagcccc ggctccccag gcactcccgg cagccgctcc
     1021 cgcaccccgt cccttccaac cccacccacc cgggagccca agaaggtggc agtggtccgt
     1081 actccaccca agtcgccgtc ttccgccaag agccgcctgc agacagcccc cgtgcccatg
     1141 ccagacctga agaatgtcaa gtccaagatc ggctccactg agaacctgaa gcaccagccg
     1201 ggaggcggga aggtgcaaat agtctacaaa ccagttgacc tgagcaaggt gacctccaag
     1261 tgtggctcat taggcaacat ccatcataaa ccaggaggtg gccaggtgga agtaaaatct
     1321 gagaagcttg acttcaagga cagagtccag tcgaagattg ggtccctgga caatatcacc
     1381 cacgtccctg gcggaggaaa taaaaagatt gaaacccaca agctgacctt ccgcgagaac
     1441 gccaaagcca agacagacca cggggcggag atcgtgtaca agtcgccagt ggtgtctggg
     1501 gacacgtctc cacggcatct cagcaatgtc tcctccaccg gcagcatcga catggtagac
     1561 tcgccccagc tcgccacgct agctgacgag gtgtctgcct ccctggccaa gcagggtttg
     1621 agctccggag gggatccacc ggtcgccacc atgtcggtac cgctgactac ctcagcattc
     1681 ggccacgcgt ttctggctaa ctgtgaacgc gagcagatcc acctggcggg ctccattcag
     1741 ccgcacggta tcctgctggc tgtgaaagag ccggacaacg tggtgatcca ggcttctatt
     1801 aacgctgcgg agttcctgaa caccaactct gttgttggcc gtccgctgcg tgacctgggc
     1861 ggcgatctgc ctttgcagat cctgccgcac ctgaacggcc cgctgcacct ggctccgatg
     1921 accctgcgtt gtaccgtggg ttctccgccg cgtcgtgtgg actgtaccat tcatcgtccg
     1981 tctaacggcg gcctgatcgt agaactggaa ccagcaacca agaccactaa cattgcgccg
     2041 gctctggacg gtgcgtttca tcgtatcact tcttcatcct ccctgatggg cctgtgtgac
     2101 gaaaccgcga ctattatccg tgagattact ggctacgacc gtgtgatggt agtacgtttc
     2161 gatgaagagg gtaatggcga aattctgtcc gaacgtcgtc gtgcggacct ggaagcgttc
     2221 ctgggtaacc gctacccggc gtctactatt ccgcagatcg ctcgtcgcct gtacgaacat
     2281 aaccgtgttc gcctgctggt agatgtgaac tatactccgg ttccgctaca gccgcgcatc
     2341 agcccgctga acggtcgtga tctggatatg tccctgtctt gcctgcgctc tatgtccccg
     2401 atccaccaga aatacatgca ggacatgggc gttggcgcga ccctggtttg ctctctgatg
     2461 gtgtctggtc gtctgtgggg tctgatcgct tgccaccact acgaaccgcg cttcgttccg
     2521 ttccacattc gcgctgctgg cgaagcgctg gcggaaactt gtgcgatccg catcgcgacg
     2581 ctggagagct ttgcacagtc tcagtccaaa tgagcggccg cgactctaga tcataatcag
     2641 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2701 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2761 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2821 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     2881 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     2941 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3001 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3061 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3121 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3181 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3241 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3301 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3361 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3421 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     3481 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3541 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3601 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3661 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3721 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3781 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     3841 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     3901 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     3961 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4021 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4081 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4141 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4201 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4261 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4321 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4381 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4441 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4501 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4561 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4621 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     4681 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4741 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4801 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     4861 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     4921 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     4981 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     5041 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     5101 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5161 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5221 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5281 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5341 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5401 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5461 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5521 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5581 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5641 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5701 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5761 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5821 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5881 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5941 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     6001 agttccg
//
