LOCUS       sequence-340277-        9666 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector to constitutively express overactive YAP (5 LATS 
            phosporylation sites mutated to Ala) under control of the human PGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Pearson JD, Huang K, Pacal M, McCurdy SR, Lu S, Aubry A, Yu T, 
            Wadosky KM, Zhang L, Wang T, Gregorieff A, Ahmad M, Dimaras H, 
            Langille E, Cole SPC, Monnier PP, Lok BH, Tsao MS, Akeno N, Schramek
            D, Wikenheiser-Brokamp KA, Knudsen ES, Witkiewicz AK, Wrana JL, 
            Goodrich DW, Bremner R
  TITLE     Binary pan-cancer classes with distinct vulnerabilities defined by 
            pro- or anti-cancer YAP/TEAD activity.
  JOURNAL   Cancer Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016.
  PUBMED    34270926
REFERENCE   2  (bases 1 to 9666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2021 Jul 13. pii: S1535-6108(21)00338-X. doi: 
            10.1016/j.ccell.2021.06.016."
COMMENT     Sequence Label: PGKp-GFP-YAP (5SA)
FEATURES             Location/Qualifiers
     source          1..9666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     40..57
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    266..290
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1806..1871)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(1891..2401)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1954..1972)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    2450..2567
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2780..2824)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3009..3242
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3735..3860
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3907..4087
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4088..4314)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4342..4362)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4342..4360)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4381..4397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4381..4397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4394..4416)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4405..4421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4429..4435,4436..4453,4454..4459))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4474..4495
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4612..4629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4783..5371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4863..4882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5542..6333,6334..6402))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6165..6184
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6403..6507)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6533..6988)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6670..6691)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6882..6901
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7115..7137
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7129..7146
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7130..7146
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7156..7175
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7156..7174
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7195..7330
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7370..7491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7381..7400)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7435..7454
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7563..7796
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(7924..8523)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8007..8027)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8504..8523
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(8524..8577)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(8587..9297,9298..9300,9301..9303))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8587..9297,9298..9300,9301..9303))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8629..8650)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8978..8997
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9237..9258
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(9304..9312,9313..9315,9316..9666,1..224)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9477..9496)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 ccagaggaac tgcttccttc acgacattca acagaccttg cattcctttg gcgagagggg
       61 aaagacccct aggaatgctc gtcaagaaga cagggccagg tttccgggcc ctcacattgc
      121 caaaagacgg caatatggtg gaaaataaca tatagacaaa cgcacaccgg ccttattcca
      181 agcggcttcg gccagtaacg ttaggggggg gggagggaga gggggggggg cggaattcga
      241 ttcgaggccg cgttttagct agatcaccac tttgtacaag aaagctgggt ctataaccat
      301 gtaagaaagc tttctttatc tagcttggtg gcagccaaaa cagactccat gtcattaagg
      361 atgtcagaac tcaaagcttc ctgcagactt ggcatcagct cctctccttc tatgttcatt
      421 ccatctcctt ccagtgttcc aaggtccaca tttgtcccag gaatggcttc aaggtagtct
      481 gggaaacggt tctgctgtga gggcagggtg ctttggttga tagtatcacc tgtatccatc
      541 tcatccacac tgttcaggaa gtcatctggg gttcgaggga cactgtagct gctcatgctt
      601 agtccactgt ctgtagcctc atctcgagag tgataggtgc cactgttaag gaaaggatct
      661 gagctattgg tcgtcattgt tctcaattcc tgagacatcc cgggagaaga cactggattt
      721 tgagtcccac catcctgctc cagtgttggt aactggctac gcagggctaa ctcctgacat
      781 tttggagaat ttgctgtgct gggattgata ttccgcattg cctgccgaag cagttcttgc
      841 tgtttcagcc gcagcctctc cttctccatc tgcagttgct gcagtcgcat ctgttgctgc
      901 tggttggagt tgctgccacc catgacgcct ccctgtgggc tctggggagc caggggtggt
      961 ggctgtttca ctggagcact ctgactgatt ctctggttca tggcaaaacg agggtcaagc
     1021 cttgggtcta gccaagaggt ggtcttgttc ttatggttta tatagtaaat ttctccatcc
     1081 tgagtcatgg cttgttccca tccatcagga agaggacctg aagccgagtt catcatattc
     1141 tgctgcactg gtggactggt gggggctgtg acgttcatct gggacagcat ggccttcctg
     1201 gggtcctgcc atgttgttgt ctgatcgatg tgatttaaga agtatctctg accagaagat
     1261 gtctttgcca tctcccaacc tgctggcaga ggtacatcat caggtatctc aaaagcagac
     1321 tgtcgaagat gctgagctgt gggtgtagct gctgggccag agactactcc agtgggggtc
     1381 agtgtcccag gagaaacagc tcccaactgc agagaagctg gagaggcatg agctcgaaca
     1441 tgctgtggag tcagggctcc tgcagtgcct gcatcagtag cggcctgtcg ggagtgggat
     1501 ttgggctccg gcggcttgaa gaaggagtcg ggcagcttcc ggagcctcat gggcacggtc
     1561 tggggcacgt tggccgtctt ggggttcatg acggcgttga agagcgcctc caggtcggtc
     1621 tccgcgtccc cgcggacgtg cacgatctga tgcccggcgg ggggtgcctg cggcgccgcc
     1681 tgggtcgccg cgggtgccgg ttgcccgggt ccggacggcg ggccctgccc ctgcgggggc
     1741 tgcgaaggcg gctgcccttg gccctggggg gccggttgag gcggcggctg ctgcccggga
     1801 tccatcttgt catcgtcatc cttgtagtcg atgtcatgat ctttataatc accgtcatgg
     1861 tctttgtagt ccatgctccg gtggatcccc ctggggagag aggtcggtga ttcggtcaac
     1921 gagggagccg actgccgacg tgcgctccgg aggcttgcag aatgcggaac accgcgcggg
     1981 caggaacagg gcccacacta ccgccccaca ccccgcctcc cgcaccgccc cttcccggcc
     2041 gctgctctcg gcgcgccctg ctgagcagcc gctattggcc acagcccatc gcggtcggcg
     2101 cgctgccatt gctccctggc gctgtccgtc tgcgagggta ctagtgagac gtgcggcttc
     2161 cgtttgtcac gtccggcacg ccgcgaaccg caaggaacct tcccgactta ggggcggagc
     2221 aggaagcgtc gccggggggc ccacaagggt agcggcgaag atccgggtga cgctgcgaac
     2281 ggacgtgaag aatgtgcgag acccagggtc ggcgccgctg cgtttcccgg aaccacgccc
     2341 agagcagccg cgtccctgcg caaacccagg gctgccttgg aaaaggcgca accccaaccc
     2401 cctcgagccg cggccaaagt ggatctctgc tgtccctgta ataaacccga aaattttgaa
     2461 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
     2521 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata
     2581 ctgccatttg tctcgaggtc gagaattgtc ccctcggggt tgggaggtgg gtctgaaacg
     2641 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
     2701 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
     2761 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
     2821 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
     2881 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
     2941 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
     3001 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
     3061 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
     3121 gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg
     3181 tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc
     3241 ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta
     3301 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
     3361 tcatatctcc tcctccaggt ctgaagatca gcggccgctt gctgtgcggt ggtcttactt
     3421 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
     3481 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
     3541 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
     3601 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
     3661 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
     3721 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
     3781 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
     3841 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
     3901 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
     3961 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
     4021 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
     4081 gagacccgtt tatgtatcga gctaggcact taaatacaat atctctgcaa tgcggcaatt
     4141 cagtggttcg tccaatccat gtcagacccg tctgttgcct tcctaataag gcacgatcgt
     4201 accaccttac ttccaccaat cggcatgcac ggtgcttttt ctctccttgt aaggcatgtt
     4261 gctaactcat cgttaccatg ttgcaagact acaagagtat tgcataagac tacattaagc
     4321 ttgcagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     4381 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4441 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4501 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     4561 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4621 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4681 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4741 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4801 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4861 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4921 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4981 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5041 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5101 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5161 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5221 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5281 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5341 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5401 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5461 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5521 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5581 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5641 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5701 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5761 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5821 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5881 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5941 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6001 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6061 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6121 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6181 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6241 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6301 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6361 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6421 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6481 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     6541 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     6601 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     6661 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     6721 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     6781 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     6841 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     6901 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     6961 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     7021 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     7081 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     7141 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacgtc cctaggcctc
     7201 caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg
     7261 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
     7321 ggggcgggat gggcggagtt aggggcggga tagctagagc cagacatgat aagatacatt
     7381 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     7441 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt tcctctcact
     7501 ctctgatatt catttctttg caagttataa atactgaata ataagatgac atgaactact
     7561 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
     7621 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
     7681 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
     7741 cccagtacaa gcaaaaagca gatcttgtct tcgttgggag tgaattagcc cttccagtcc
     7801 ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa
     7861 aggtaccgat gcatggggtc gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg
     7921 gcgtcaggca ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac
     7981 gtcggcggtg acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt
     8041 ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc
     8101 gctgcccaga cccttgccct ggtggtcggg cgagacgccg acggtggcca ggaaccacgc
     8161 gggctccttg ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg
     8221 ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac
     8281 gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc
     8341 gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg
     8401 gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt
     8461 gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt
     8521 catgggtcca ggattctcct cgacgtcacc gcatgttagc agacttcctc tgccctctcc
     8581 gctgcccttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
     8641 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag
     8701 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
     8761 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
     8821 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
     8881 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
     8941 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
     9001 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
     9061 ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac
     9121 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
     9181 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc
     9241 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac
     9301 catggttgtg gccatattat catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg
     9361 gggggcctag acgttttttt aacctcgact aaacacatgt aaagcatgtg caccgaggcc
     9421 ccagatcaga tcccatacaa tggggtacct tctgggcatc cttcagcccc ttgttgaata
     9481 cgcttgagga gagccatttg actctttcca caactatcca actcacaacg tggcactggg
     9541 gttgtgccgc ctttgcaggt gtatcttata cacgtggctt ttggccgcag aggcacctgt
     9601 cgccaggtgg ggggttccgc tgcctgcaaa gggtcgctac agacgttgtt tgtcttcaag
     9661 aagctt
//
