LOCUS       sequence-340318-       10054 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encoding dog Egf..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10054)
  AUTHORS   Lin S, Hirayama D, Maryu G, Matsuda K, Hino N, Deguchi E, Aoki K, 
            Iwamoto R, Terai K, Matsuda M
  TITLE     Redundant roles of EGFR ligands in the ERK activation waves during 
            collective cell migration.
  JOURNAL   Life Sci Alliance. 2021 Oct 19;5(1). pii: 5/1/e202101206. doi: 
            10.26508/lsa.202101206. Print 2022 Jan.
  PUBMED    34667080
REFERENCE   2  (bases 1 to 10054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2021-10-19"; volume: "5(1). pii: 5/1/e202101206. 
            doi"; pages: " 10.26508/lsa.202101206. Print 2022 Jan"
COMMENT     Sequence Label: pPBpuro-UbC-canisEgf
FEATURES             Location/Qualifiers
     source          1..10054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        241..1452
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1289..1308
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    5197..5746
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5341..5358)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5568..5587
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5749..6348
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5749..5768)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6245..6265
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6375..6392)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6381..6605
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6652..6668)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6676..6692
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6700..6706,6707..6724,6725..6730))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6745..6766
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7000..7062
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7103..7123)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7103..7121)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7142..7158)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7166..7182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7190..7196,7197..7214,7215..7220))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7235..7256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7373..7390)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7544..8132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7624..7643)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8303..9094,9095..9163))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8926..8945
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9164..9268)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9294..9749)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9431..9452)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9643..9662
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9876..9898
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9890..9907
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9891..9907
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9917..9936
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9917..9935
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   9965..9999
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ttaggacatc tcagtcgccg cttggagctc ccgtgaggcg tgcttgtcaa tgcggtaagt
       61 gtcactgatt ttgaactata acgaccgcgt gagtcaaaat gacgcatgat tatcttttac
      121 gtgactttta agatttaact catacgataa ttatattgtt atttcatgtt ctacttacgt
      181 gataacttat tatatatata ttttcttgtt atagatatca actagaatgc tagcctcgag
      241 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
      301 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      361 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      421 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      481 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      541 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      601 cgcagttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      661 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaggcgtgtg gagagaccgc
      721 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      781 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      841 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      901 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      961 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
     1021 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
     1081 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
     1141 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
     1201 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctctggtcg
     1261 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1321 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1381 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1441 tttttgttag acggatccat gaattcacca tgctgctccc ccttatcatt ctgtggccgg
     1501 tagtttttaa atgtagtttt gctagtctct cagacccgga gaactggaac tgtcctgaag
     1561 tctctccctc aggaaagggg agccctgctt gtgtgggtcc tgcacccttc ttaattttct
     1621 cccatggaat cagtatcttt aggattgacc tggaaggcac taatcatgag caattggtgg
     1681 cagatgctgg tgtatcagtg atcatggatt ttcattataa taaagaaaga atctattggg
     1741 tagatccaga aagacaactt ttgcaaagag tttttctgaa tgggacaagg caagagagag
     1801 tatgcaatat agagaaaaat gtttcaggaa tggcaataaa ttggataaat gaagaactta
     1861 tttggtcaaa tcaacaggaa ggaatcatca cagtaacaga tatgaaagga aacaattcgc
     1921 gagttctctt aagagcctta aactatcctg caaatgtagc aattgatcca atagaaaggt
     1981 ttatattttg gtcttcagag gtggcagtgg ctggcagcct tcacagagca gatctcaatg
     2041 gtgtggaaga gaagattctt ttacagacat cagaaagaat aacagctgtg tcattggatg
     2101 tgcttgataa acagcttttt tggattcagt acagcagaga tggaagcaat tctcatattt
     2161 attcctgtaa ttatgatgga ggttctgtcc atcttagcaa acatcttaca cagcataatt
     2221 tttttgcaat gtcccttttt gggaatcaga tcttctattc aacatggaaa aagaagacaa
     2281 tttggatagc taacaaacac agtgggaagg atatggttag aattaaccta gattcatcat
     2341 ttgtaccacc tggtggaatc aaagtagtgc atccactctt acagcccaag gcagagagtg
     2401 gcacttgggc acctgatcag aaactctgca aatggaagca aggtaactgc agaggcagca
     2461 cgtgtggtca agattcaaag tcctattcat gcacgtgtgc agagggatat actttaagcc
     2521 aagatggaaa atactgtgaa gatgtcaatg aatgtgcctt ttggaatcat ggctgtactc
     2581 ttgggtgtga aaacatccct ggatcttatt attgcacatg ccctgtagga tttattctgc
     2641 ttcctgatgg gaaacggtgt catcaattaa ttgcctgtcc gagcaataca tctaaatgta
     2701 gccatgactg tgttctgaca tcagatggtc ccatatgttt ctgtcctgaa ggctcagtgc
     2761 ttgaggcaga tggaaaaaca tgtagtggct gttcatcacc tgataatggt ggatgtagcc
     2821 agctctgcct ccctctcagc ccagtatcct gggaatgtgg ttgctttcct gggtatgacc
     2881 tacaactgga caaacaaagc tgtgcagcat caggaccaca accatttttg ctgtttgcca
     2941 attctcaaga tattcgacac atgcattttg atggaacaga ttatggaacc ctgctcagcc
     3001 agcaaatggg aatggttttt gcccttgatc acgaccctgt ggaaaataag atatactttg
     3061 cccatacagc cctgaagtgg atagagagag ctaacatgga tggttcccag cgagaaaggc
     3121 ttattgagga aggagtggat gtgccagaag gtcttgccat agattggatt gaccgtaaat
     3181 tctactggac agacagcgga aaatctctta ttgaagggag tgatttaaat ggaaaacatc
     3241 gtgaggtaat catcaaggaa gacatctctc agccacgagg aattgctgtt catccaatgg
     3301 ccaagagatt attctggact gatatgggga ttaatccacg aattgaaagt tcttcccttc
     3361 aaggcattgg ccgactggtt atagctagct ctgatctggt ctggcccagt ggaataacga
     3421 ttgattatgt aactgacaaa ttgtactggt gtgataccaa gctgtctgtg attgagatgg
     3481 ccaatctgga tggttcaaaa cgccaaagac ttgcccagaa cgatgtaggt cacccatttg
     3541 ctatggccgt gtttgaggat cacgtgtggt tctctgattg gactatgcca tcaataataa
     3601 gagtggacaa gaggactggc aaaaacaggg tacgtctccg aggcagcatg ctgaagcctt
     3661 catcactggt tgtagttcat ccattggcaa aaccaggagc acagccctgc ttatatcaaa
     3721 atggaggctg tgaacatatt tgcaaagaga ggtttggaac tgctcaatgt ttgtgtcgtg
     3781 aaggttttgt gaaagcccca gatgggaaaa tgtgtctggc tctgaatggc catcagatac
     3841 cggcagtagg tagtgaagca gatctaagta atcacgtaac gccaggggat gtcttaccca
     3901 gaagtgaagg atttgaagat aacattacag aatctcagca tatgctagtg gccgaaatca
     3961 tggtgtcaga tgctgacgac tgtgctcctg tgggatgcag tacatgggct gagtgtgttt
     4021 cagagggaga aaatgccaca tgtcagtgtt tgaaaggatt tactggggat ggaaagctat
     4081 gttttgacat agatgaatgt gagatgggca tcacgatttg ccctcctacc tcctcaaagt
     4141 gcgtcaatac tgaaggtggt tatgtttgtc agtgctcaga aggctaccga ggcgatggga
     4201 tccactgtct ggatattaat gagtgccaac tgggcatgca cacctgtggg gaaaatgcca
     4261 cctgtacaaa tatggaggga aactatacct gcatgtgcgc tggcagcctg tctgaacctg
     4321 gacagatatg tgctgactct actccgcctt ctcatcccat ggaggacagt cactattctg
     4381 tgagaaatgg ttatcgggaa tgcccctcat cctatgatgg gtactgcctc tataatggtg
     4441 tgtgtatgta cattgaagca gtcgacagat acgcatgcaa ctgtgttttt ggctacgtcg
     4501 gggagcgatg tcagcaccga gacctgaaat gggaactgcg ccacgcgggc cagggccggc
     4561 agcggcaggt cgccgcggtg gccgtgggcg tggccgtgct cgtcctgctg ctgctgctcg
     4621 ggctgggggg cgcgcactgc tacaggacta agaagttgtc atcaaaaaat ttaaagaatc
     4681 cttatgaaga gccaagcaga gagggtagca gtagcaggcc ttcagacagc gaggctagga
     4741 tggcctcttt tccccaacct tggtttgtgg ttataaagga acatcaaaat ctcaggaatg
     4801 gaagtcaacc tatggccctc aaggatggtg agtcagcaga tgttagccaa ttttcctctc
     4861 cagagccagg gtcagtaaaa cggacctcat ggagaaatga acaccagtta tataaggaca
     4921 cagagcaagg ctgctgcact ccaccatcca gtaatagagg caccggctct cagtcaatgg
     4981 agcagagctt ttctgtcccc tcctatgagg cacagcccat tgctttgggg gttgagaagc
     5041 cacagtctct cctatcagct aacccttatt gcaacaaagg gccccagatc caccacacca
     5101 aatgaagctg attcagtgag cggccgctct agagtcaact aacttaagct agcaacggtt
     5161 tccctctagc gggatcaatt ccgccccccc cccctaacgt tactggccga agccgcttgg
     5221 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     5281 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     5341 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     5401 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     5461 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     5521 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     5581 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     5641 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     5701 ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat gaccgagtac
     5761 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     5821 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     5881 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     5941 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6001 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6061 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6121 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6181 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6241 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6301 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatc gatgatctag
     6361 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     6421 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     6481 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     6541 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     6601 tatggcttct gaggcggaaa gaacctgcag cccaagcttg gcgtaatcat ggtcatagct
     6661 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6721 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6781 actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atcttaaaag ttttgttact
     6841 ttatagaaga aattttgagt ttttgttttt ttttaataaa taaataaaca taaataaatt
     6901 gtttgttgaa tttattatta gtatgtaagt gtaaatataa taaaacttaa tatctattca
     6961 aattaataaa taaacctcga tatacagacc gataaaacac atgcgtcaat tttacgcatg
     7021 attatcttta acgtacgtca caatatgatt atctttctag ggttaatcta gtatacgcgt
     7081 ttaattaatc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     7141 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7201 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7261 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7321 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7381 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7441 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7501 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7561 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7621 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7681 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7741 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7801 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7861 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7921 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7981 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8041 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8101 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8161 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8221 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8281 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8341 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8401 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8461 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8521 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8581 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8641 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8701 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8761 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8821 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8881 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8941 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9001 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9061 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9121 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9181 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9241 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     9301 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     9361 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     9421 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     9481 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     9541 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     9601 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     9661 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     9721 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     9781 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     9841 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     9901 ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggggcgcg ccattctaga
     9961 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
    10021 tctttctaaa tagcgcgaat ccgtcgctgt gcat
//
