LOCUS       sequence-340496-       17486 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Encodes OptoQ-INVRT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17486)
  AUTHORS   Lalwani MA, Zhao EM, Wegner SA, Avalos JL
  TITLE     The Neurospora crassa Inducible Q System Enables Simultaneous 
            Optogenetic Amplification and Inversion in Saccharomyces cerevisiae 
            for Bidirectional Control of Gene Expression.
  JOURNAL   ACS Synth Biol. 2021 Aug 20;10(8):2060-2075. doi: 
            10.1021/acssynbio.1c00229. Epub 2021 Aug 4.
  PUBMED    34346207
REFERENCE   2  (bases 1 to 17486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00229"; journalName: "ACS Synth Biol"; date: 
            "2021-08-20- 20"; volume: "10"; issue: "8"; pages: "2060-2075"
COMMENT     Sequence Label: pMAL513
FEATURES             Location/Qualifiers
     source          1..17486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        314..501
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        2083..2114
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     terminator      complement(7314..7479)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     7400..7424
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(7495..8202)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(7535..7555)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     8150..8178
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     promoter        complement(11586..11617)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     terminator      complement(11788..12035)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     12001..12019
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(12924..12944)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(12974..13371)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(14034..14053)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14035..14053)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14060..14077)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14060..14076)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14069..14091)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      14217..14672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14304..14323)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14514..14535
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(15028..15047)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15147..15169
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(15207..15225)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        15309..15413
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15414..15482,15483..16274)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15632..15651)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      16445..17033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16934..16953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17187..17204
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    17321..17342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(17357..17362,17363..17380,17381..17387)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17395..17411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     17400..17422
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     17419..17435
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17419..17435
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        17456..17474
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgct gcacggtcct gttccctagc
       61 atgtacgtga gcgtatttcc ttttaaacca cgacgctttg tcttcattca acgtttccca
      121 ttgttttttt ctactattgc tttgctgtgg gaaaaactta tcgaaagatg acgacttttt
      181 cttaattctc gttttaagag cttggtgagc gctaggagtc actgccaggt atcgtttgaa
      241 cacggcatta gtcagggaag tcataacaca gtcctttccc gcaattttct ttttctatta
      301 ctcttggcct cctctagtac actctatatt tttttatgcc tcggtaatga ttttcatttt
      361 tttttttcca cctagcggat gactcttttt ttttcttagc gattggcatt atcacataat
      421 gaattataca ttatataaag taatgtgatt tcttcgaaga atatactaaa aaatgagcag
      481 gcaagataaa cgaaggcaaa gaggctctat atgtatataa agctgcggat gccttctgat
      541 ttaatctagt tctagtttac ttcaggttta cttaaagttg agataattga agcaaagaga
      601 agcatcgagc aaaggatggc gtttgttaag agggttacgc aggagacgaa tatacagctg
      661 gcgctggatc ttgacggtgg gtctgtttct gtacgggaga gcatactggg caaggaatat
      721 gctagtggtg atgggcagac catccatgtg cacactggag ttgggttttt ggaccacatg
      781 ttgactgcgc tggcgaagca tggcgggtgg tctctgatcc tggagtgtat aggggacttg
      841 cacatcgatg accaccacac tgttgaggac tgtgggattg cgctgggcca agcgttcaag
      901 gaggcgcttg gctccgtgcg tggtatcaag aggttcgggc atgggtttgc accactggac
      961 gaggcgctga gccgcgctgt ggttgacttc tccaataggc ctttcgccgt ggtggagctg
     1021 ggcctgaagc gagaacgcat aggccagcta tccacagaga tgatcccgca cttcttggag
     1081 agtttcgcca ctgaggcgcg tatcaccatg catgtggact gtctgcgggg caccaacgac
     1141 caccaccgct ccgaatcagc tttcaaggcg ctcgccatcg ccatcagaga ggcaagaaca
     1201 cctacgggtc gcgatgacgt tccatccact aagggtgtcc tagcatagaa cacagcccac
     1261 agctaccacc atcaacaata tttatataga taacgtacac atagaaatca cacaaacaga
     1321 gtatttattc ttaactacat gaactaccat cagaccgtct gggccactat ataatgtgct
     1381 attcataaac gtgatcactc tacgtagcag gcgaccccag gtgaaaattt ttcagcgagc
     1441 tgccagattg tcaggtgaaa aactgaaaaa aacttctggg cgatgagctt gtggcgggaa
     1501 attaagtata taaagcagtt agtttcttct gcttcttgtg gttctgggta ttcttgtaat
     1561 atttggaata atagaaaacc aagactactg cctttctttt catattatag aggaagaaat
     1621 acgcacgaac acgatataga ggtaaatttt ttgcaaaaag ataccaaact ataaaagaaa
     1681 tagaagacta aactacaaaa aataaagaaa gtaatacata tatccaatat gactgagtta
     1741 gcagagcaag tttccatgcc tgaagggcaa caaagacgtg gtggttacgt tccacctcac
     1801 atccgtaacg gtggtggcag aagaggcgat tctgctcctc gtggtggttt caacaacggt
     1861 ggcggccgtg gtggtttctt cagaaacaac ggtgaccgtg gtggattcgg ctttgggtac
     1921 ctccgggtac gtgagttcgc cagcttcgag taggtagcct ttagtccatg cgttataggt
     1981 agcctttagt ccatgcgtta taggtagcct ttagtccatg cgttataggt agcctttagt
     2041 ccatgcgtta taggtagcct ttagtccatg cttaagagac actagagggt atataatgga
     2101 agctcgactt ccagcttggc aatccggtac tgttggtaaa gccaccgcgg ccgctaaaat
     2161 catgaacacc attccagcta gacacgtcgg cgacgttgcc gccagagatc ctttaccact
     2221 accccacatc tctagctccg tagcatctgg tatgaagcgt agcttcgcaa caatggcgat
     2281 gctgtataac gacaccggca acagcaatga cgttggagcc catgcgagaa gacctccaag
     2341 gaccctgagt aatagcaggt caaccagcgc gcatagagtc ccgctaggaa gctggtccgc
     2401 accgaactca cctccgaggc gtgccttgcc acatcatcca attaccgcgt ccttcgaccc
     2461 tgatgctagc attgtcattg cggggatcag gggggctggg aagagcacgc ttgcaattat
     2521 ggcaagcaca gcgatgaaaa gaaaaattgt agacttagaa tctgaattcc accatttgac
     2581 gggattgagt agttcaagct ataaaaaaac gcatggtcct gttgattatg gcagaaggca
     2641 aatcgccatc ttacaaaaca tcctgaattt gcataggact agagctattt tggtatgtag
     2701 ttggcttgag agagatgtac aggcaatgct gcaagacttt agtgtgagta atcctgtcat
     2761 ctatgttctg agagatgcga aggctatcga ggcacacctg aagggatatg ataagtctaa
     2821 ggtgggcacg cttttggacg ctacctcaac agttcttagg aggtgcaccc gttttgaatt
     2881 tttcaacgtt tcagaggaaa acctagatac ccactccgca agtactagtc ctccagctgt
     2941 ccccgatcag aggcacaccg ccccatatct taccctaaaa cgtgcggaaa gacactttct
     3001 gaagttttta agtcttatac tacccaaggg gacaatacct ttcgtagaaa gtgcgttccc
     3061 gctagcctct gtaccagttg aacagcgtcg tttcacctat gctctagctt tacctgtatc
     3121 agctctgctt gacaaagggg ttgatattca ggagttggat gttggagtcg atgccataga
     3181 gattatcgtg gacgatcttg caacttccga atctggacct acaagccccc tgggcttagc
     3241 tccgcacagg gcttcagaaa taagccgtgt cgtgggcgag attagaagag atactgttat
     3301 acctattatc cttcatgttg tgttccctga gagggcccta tatgaggaag cccttctagc
     3361 gttgtacatg acttatctta accatgcact taggcttgca cccgactacc tgaccgtaga
     3421 ccttgggctt gacagcggtt tgttaggaca gctaacaacc gtccaaggga ccacgaaagt
     3481 gataggaaat aaacaacttg cagaggtaaa ctccccgaga tggggtgatc cgagttggtt
     3541 acaagcatac gagaaagcac aaaacacagg gtgtgattta gtgaggttga ccagacccgc
     3601 tagcaatccc cgtgataaca cagatattag acagttccat gttgcagttg aggcggttgg
     3661 gggacctagg ctgccattta tcgcttataa caccgggaga ttgggaagaa cgtctatgtg
     3721 ctttaatgag atcctgaccc ccgtcacccc agtaccgacg aaggaggatg cgataggcct
     3781 tcgtaatccg gctcacagat acttacagcc tcctctaaca gcactggaag ccacacaagc
     3841 cctttattcc gcgtttgtgc acgatcctat gaaattatat gtgttcgggg ctaacgttgg
     3901 ctactccctt tcaccggcta tgcacaacgc agcgctaaaa gcttgcggaa taccacacca
     3961 ctataaaccg ctatcaactg ctaatatagg gactttgagg gaagtaatta gtgatccaca
     4021 atttgctgga gcttcagtag ggttaccgtt caaagttgag attatttcac ttacacatag
     4081 tctgtctaga cacgcaaagg ctatcggagc cgtgaatacg ttaatcccag tgcgtcatct
     4141 tactgcggac gggggcattc cagatgaagt cagtatgttt aataacattt ctcaggcggg
     4201 agcagtgaga gctctttatg gtgaaaatac tgattggatt ggaataagag catgtttaag
     4261 aagggggctt agcccagcaa acgcagtgag gtctacaagc accggtttag tgataggtgc
     4321 cggggggatg gcgagagccg cagtgtatgc aatgctgcaa cttggggtca aaaaaatttt
     4381 aattttcaac cgtactttcg ctaatgctga aaaactggtt cttcatttcg aaaacctgtt
     4441 agttcgtgat gcacttccat tgttaagcac ggggcccagg agtcatgata atacgtgctt
     4501 tcatatcatc cgttctagag atgacccatt gccagagaat ttcaaaaatc ctactatgat
     4561 cgtgagctgt attccaaccc atacagtcga taacacaccg gacccggaat ttacagtccc
     4621 cctacactgg cttgacaatc ctaccggcgg tatagttcta gagttagatt ataagtgctt
     4681 aacctcccca ctgctagagc agacacgtcg tgaagctcac cgtggctggg tggccatgga
     4741 cggacttgac ttactgcctg aacaaggctt tgcccagttc gaactgttta caggtagacg
     4801 tgcgccgcgt aggttgatga ggcgtgaagt gctacgtgcg tatccagacg accaggctaa
     4861 aagtcacacg gcacaactac agccccgtct aaacgggata gccacgcaaa tctctctcga
     4921 gagcttttga ttaagccttc tagtccaaaa aacacgtttt tttgtcattt atttcatttt
     4981 cttagaatag tttagtttat tcattttata gtcacgaatg ttttatgatt ctatataggg
     5041 ttgcaaacaa gcatttttca ttttatgtta aaacaatttc aggtttacct tttattctgc
     5101 ttgtggtgac gcgtgtatcc gcccgctctt ttggtcaccc atgtatcgcc gggaaacagc
     5161 tgcaagaatt actcgtgagt aaggaaagag tgaggaacta tcgcatacct gcatttaaag
     5221 atgccgattt gggcgcgaat cctttatttt ggcttcaccc tcatactatt atcagggcca
     5281 gaaaaaggaa gtgtttccct ccttcttgaa ttgatgttac cctcataaag cacgtggcct
     5341 cttatcgaga aagaaattac cgtcgctcgt gatttgtttg caaaaagaac aaaactgaaa
     5401 aaacccagac acgctcgact tcctgtcttc ctattgattg cagcttccaa tttcgtcaca
     5461 caacaaggtc ctagcgacgg ctcacaggtt ttgtaacaag caatcgaagg ttctggaatg
     5521 gcgggaaagg gtttagtacc acatgctatg atgcccactg tgatctccag agcaaagttc
     5581 gttcgatcgt actgttactc tctctctttc aaacagaatt gtccgaatcg tgtgacaaca
     5641 acagcctgtt ctcacacact cttttcttct aaccaagggg gtggtttagt ttagtagaac
     5701 ctcgtgaaac ttacatttac atatatataa acttgcataa attggtcaat gcaagaaata
     5761 catatttggt cttttctaat tcgtagtttt tcaagttctt agatgctttc tttttctctt
     5821 ttttacagat catcaaggaa gtaattatct actttttaca acaaatataa aacaccgcgg
     5881 tggcggccgc taaaatcatg ccacccaaga gaaagacgct gaacgcggca gcggaagcta
     5941 acgcccatgc agatggccac gcggacggta atgcggacgg tcatgtagct aacaccgcgg
     6001 cttcctctaa caacgcacgt ttcgctgact tgacgaatat cgatacgcca gggttgggac
     6061 cgactactac caccttattg gtggagcctg ccaggtctaa aagacaaagg gtcagtcgtg
     6121 cgtgtgacca atgcagagcc gctcgtgaaa aatgtgacgg tattcagcct gcatgttttc
     6181 cttgcgtgag tcagggcagg tcttgcacgt atcaggcttc ccccaagaag cgtggcgttc
     6241 agactggtta tattaggacc ttggaattgg cgttggcgtg gatgtttgaa aacgtagcaa
     6301 ggtccgaaga cgcgctacat aatctattag tcagggacgc tggccaaggc tcagcgttac
     6361 tggtagggaa ggattcaccg gcggcggaaa gactgcatgc aaggtgggcg acctccaggg
     6421 tcaataaaag tatcacaagg cttctccgtc aattagagct gccgcccacg gctactgcca
     6481 ctgcgtcaat catgcctcac gtcatggaac aacccttgag tacgtcaata aaccctgtta
     6541 atgatcgttt taatgggatt ccaaatccga cgccttacaa ttctgacgct gctcttgacg
     6601 ctataaccca aaccaacgat tacggttccg tgaataccca cggaatcctg agcacctacc
     6661 cccctccagc aacacacctt aacgaggcgt cagtagctct agctccggga ggggcgcctc
     6721 cgcgtccacc cccaccgtac gtggactcaa cgactaatca ccctccctat catagcaacc
     6781 tggtccctat ggcaaatttt ggttacagta cagttgatta tgatgccatg gtagatgatt
     6841 tagcgagtat tgaatatact gatgctgtag atgttgaccc acaatttatg acgaatcttg
     6901 ggttcgtccc tgggtgcaat ttcagtgaca ttaacacgta tgaacaataa tgactcgagt
     6961 aatgatcatc tctgcttttg tgcgcgtatg tttatgtatg tacctctctc tctatttcta
     7021 tttttaaacc accctctcaa taaaataaaa ataataaagt atttttaagg aaaagacgtg
     7081 tttaagcact gactttatct actttttgta cgttttcatt gatataatgt gttttgtctc
     7141 tcccttttct acgaaaattt caaaaattga ccaaaaaaag gaatatatat acgaaaaact
     7201 attatattta tatatcatag tgttgataaa aaatgtttat ccattggacc gtgtatcata
     7261 tttatcgccg gcgaataaat taaggcgcgc cttaatttat tcgccggcga taagagcgac
     7321 ctcatgctat acctgagaaa gcaacctgac ctacaggaaa gagttactca agaataagaa
     7381 ttttcgtttt aaaacctaag agtcacttta aaatttgtat acacttattt tttttataac
     7441 ttatttaata ataaaaatca taaatcataa gaaattcgct gatcattact cgagtttgta
     7501 tagttcatcc atgccatgtg taatcccagc agctgttaca aactcaagaa ggaccatgtg
     7561 gtctctcttt tcgttgggat ctttcgaaag ggcagattgt gtggacaggt aatggttgtc
     7621 tggtaaaagg acagggccat cgccaattgg agtattttgt tgataatggt ctgctagttg
     7681 aacgcttcca tcttcaatgt tgtgtctaat tttgaagtta actttgattc cattcttttg
     7741 tttgtctgcc atgatgtata cattgtgtga gttatagttg tattccaatt tgtgtccaag
     7801 aatgtttcca tcttctttaa aatcaatacc ttttaactcg attctattaa caagggtatc
     7861 accttcaaac ttgacttcag cacgtgtctt gtagttcccg tcatctttga aaaatatagt
     7921 tctttcctgt acataacctt cgggcatggc actcttgaaa aagtcatgcc gtttcatatg
     7981 atctgggtat cttgaaaagc attgaacacc ataagtgaaa gtagtgacaa gtgttggcca
     8041 tggaacaggt agttttccag tagtgcaaat aaatttaagg gtaagttttc cgtatgttgc
     8101 atcaccttca ccctctccac tgacagaaaa tttgtgccca ttaacatcac catctaattc
     8161 aacaagaatt gggacaactc cagtgaaaag ttcttctcct ttgtcgacgc tagccatgat
     8221 tttagcggcc gctattaatt tagtgtgtgt atttgtgttt gtgtgtctat agaagtatag
     8281 taatttatgc tgcaaaggtc ctaatgtata aggaaagaat atttagagaa aagaagaaaa
     8341 caagagtttt atatacatac agagcacatg catgccatat gatcatgtgt cgtcgcacac
     8401 atatatatat ctagaaagga taagcgatta ccctaggata agcgattacc ctagaggata
     8461 agcgattacc cttgaggata attgtttatc ctaggataag cgattaccca ctagtttaat
     8521 ttattcttaa gctggcccgg cgataacccg gcgatacatg ggtgaccaaa agagcgggcg
     8581 gatacacgcg tcaccacaag cagaataaaa ggtaaacctg aaattgtttt aacataaaat
     8641 gaaaaatgct tgtttgcaac cctatataga atcataaaac attcgtgact ataaaatgaa
     8701 taaactaaac tattctaaga aaatgaaata aatgacaaaa aaacgtgttt tttggactag
     8761 aaggcttaat caaaagctct cgagagagat ttgcgtggct atcccgttta gacggggctg
     8821 tagttgtgcc gtgtgacttt tagcctggtc gtctggatac gcacgtagca cttcacgcct
     8881 catcaaccta cgcggcgcac gtctacctgt aaacagttcg aactgggcaa agccttgttc
     8941 aggcagtaag tcaagtccgt ccatggccac ccagccacgg tgagcttcac gacgtgtctg
     9001 ctctagcagt ggggaggtta agcacttata atctaactct agaactatac cgccggtagg
     9061 attgtcaagc cagtgtaggg ggactgtaaa ttccgggtcc ggtgtgttat cgactgtatg
     9121 ggttggaata cagctcacga tcatagtagg atttttgaaa ttctctggca atgggtcatc
     9181 tctagaacgg atgatatgaa agcacgtatt atcatgactc ctgggccccg tgcttaacaa
     9241 tggaagtgca tcacgaacta acaggttttc gaaatgaaga accagttttt cagcattagc
     9301 gaaagtacgg ttgaaaatta aaattttttt gaccccaagt tgcagcattg catacactgc
     9361 ggctctcgcc atccccccgg cacctatcac taaaccggtg cttgtagacc tcactgcgtt
     9421 tgctgggcta agcccccttc ttaaacatgc tcttattcca atccaatcag tattttcacc
     9481 ataaagagct ctcactgctc ccgcctgaga aatgttatta aacatactga cttcatctgg
     9541 aatgcccccg tccgcagtaa gatgacgcac tgggattaac gtattcacgg ctccgatagc
     9601 ctttgcgtgt ctagacagac tatgtgtaag tgaaataatc tcaactttga acggtaaccc
     9661 tactgaagct ccagcaaatt gtggatcact aattacttcc ctcaaagtcc ctatattagc
     9721 agttgatagc ggtttatagt ggtgtggtat tccgcaagct tttagcgctg cgttgtgcat
     9781 agccggtgaa agggagtagc caacgttagc cccgaacaca tataatttca taggatcgtg
     9841 cacaaacgcg gaataaaggg cttgtgtggc ttccagtgct gttagaggag gctgtaagta
     9901 tctgtgagcc ggattacgaa ggcctatcgc atcctccttc gtcggtactg gggtgacggg
     9961 ggtcaggatc tcattaaagc acatagacgt tcttcccaat ctcccggtgt tataagcgat
    10021 aaatggcagc ctaggtcccc caaccgcctc aactgcaaca tggaactgtc taatatctgt
    10081 gttatcacgg ggattgctag cgggtctggt caacctcact aaatcacacc ctgtgttttg
    10141 tgctttctcg tatgcttgta accaactcgg atcaccccat ctcggggagt ttacctctgc
    10201 aagttgttta tttcctatca ctttcgtggt cccttggacg gttgttagct gtcctaacaa
    10261 accgctgtca agcccaaggt ctacggtcag gtagtcgggt gcaagcctaa gtgcatggtt
    10321 aagataagtc atgtacaacg ctagaagggc ttcctcatat agggccctct cagggaacac
    10381 aacatgaagg ataataggta taacagtatc tcttctaatc tcgcccacga cacggcttat
    10441 ttctgaagcc ctgtgcggag ctaagcccag ggggcttgta ggtccagatt cggaagttgc
    10501 aagatcgtcc acgataatct ctatggcatc gactccaaca tccaactcct gaatatcaac
    10561 ccctttgtca agcagagctg atacaggtaa agctagagca taggtgaaac gacgctgttc
    10621 aactggtaca gaggctagcg ggaacgcact ttctacgaaa ggtattgtcc ccttgggtag
    10681 tataagactt aaaaacttca gaaagtgtct ttccgcacgt tttagggtaa gatatggggc
    10741 ggtgtgcctc tgatcgggga cagctggagg actagtactt gcggagtggg tatctaggtt
    10801 ttcctctgaa acgttgaaaa attcaaaacg ggtgcacctc ctaagaactg ttgaggtagc
    10861 gtccaaaagc gtgcccacct tagacttatc atatcccttc aggtgtgcct cgatagcctt
    10921 cgcatctctc agaacataga tgacaggatt actcacacta aagtcttgca gcattgcctg
    10981 tacatctctc tcaagccaac tacataccaa aatagctcta gtcctatgca aattcaggat
    11041 gttttgtaag atggcgattt gccttctgcc ataatcaaca ggaccatgcg tttttttata
    11101 gcttgaacta ctcaatcccg tcaaatggtg gaattcagat tctaagtcta caatttttct
    11161 tttcatcgct gtgcttgcca taattgcaag cgtgctcttc ccagcccccc tgatccccgc
    11221 aatgacaatg ctagcatcag ggtcgaagga cgcggtaatt ggatgatgtg gcaaggcacg
    11281 cctcggaggt gagttcggtg cggaccagct tcctagcggg actctatgcg cgctggttga
    11341 cctgctatta ctcagggtcc ttggaggtct tctcgcatgg gctccaacgt cattgctgtt
    11401 gccggtgtcg ttatacagca tcgccattgt tgcgaagcta cgcttcatac cagatgctac
    11461 ggagctagag atgtggggta gtggtaaagg atctctggcg gcaacgtcgc cgacgtgtct
    11521 agctggaatg gtgttcatga ttttagcggc cgcggtggct ttaccaacag taccggattg
    11581 ccaagctgga agtcgagctt ccattatata ccctctagtg tctcttaagc atggactaaa
    11641 ggctacctat aacgcatgga ctaaaggcta cctataacgc atggactaaa ggctacctat
    11701 aacgcatgga ctaaaggcta cctataacgc atggactaaa ggctacctac tcgaagctgg
    11761 cgaactcacg tacccggcga taaggccgca aattaaagcc ttcgagcgtc ccaaaacctt
    11821 ctcaagcaag gttttcagta taatgttaca tgcgtacacg cgtctgtaca gaaaaaaaag
    11881 aaaaatttga aatataaata acgttcttaa tactaacata actataaaaa aataaatagg
    11941 gacctagact tcaggttgtc taactccttc cttttcggtt agagcggatg tggggggagg
    12001 gcgtgaatgt aagcgtgaca taactaatta catgatcatt actcgaggtc gacttaaata
    12061 cctgcttcta cggcaattct aactaaatct gcactcgttt ttaagtttaa tttttccatt
    12121 accaaacctc tgtgcatttt gacagttttt tctgaaagac ccagtctggc agccacttct
    12181 ttatttctta ggcctgatgc aaccagagtt gtgacttcta attgacgtgg tgaaagagtt
    12241 tttaacatct ccgcagcacg ttctctacgg gccatgccca tgttcggttg atcgtcatcc
    12301 acttccactt ggctaccaag gaagtatagt aattcatcat cgtcatcata gattggtgcc
    12361 accaaaactg cattacgaaa aggagtacca tcctttttgt aattcaaaat ctcaaccaac
    12421 acgggtttat gctcacgaac accttgacgg atcttatctg ttagccaggg ttcagtacca
    12481 gatcctgcca aaaatctaca attacgacca acacattctt cctcagaata cccagttaga
    12541 tcggtaaagg cctggttgat agcaatcaaa ggattatcag ccaatctggg atcactaaca
    12601 acacttgcta tgggtgaggc ctcgatcaaa tccaataccc attgggctgg aggttggact
    12661 tcaactctag tatcatctgc tccgaattca cctccatatt cgtcgatacc aagtgcatca
    12721 gtaaacatct gttcaaattc gaaatctgcc atgtccaacg cgccatacgg agcagaatca
    12781 tgtggtgtaa atccggggcc gggagaatcc ccatcaccta acatgtccag atcaaaatca
    12841 tcaagagcat cggcatgtgc catagctaca tcctcaccgt ctaaatgtaa ttcgtcacca
    12901 agtgaaacgt ccgttggtgg ggctactttt ctcttttttt tcgggctagc catgatttta
    12961 gcggccgcca aaacttagat tagattgcta tgctttcttt ctaatgagca agaagtaaaa
    13021 aaagttgtaa tagaacaaga aaaatgaaac tgaaacttga gaaattgaag accgtttatt
    13081 aacttaaata tcaatgggag gtcatcgaaa gagaaaaaaa tcaaaaaaaa aattttcaag
    13141 aaaaagaaac gtgataaaaa tttttattgc ctttttcgac gaagaaaaag aaacgaggcg
    13201 gtctcttttt tcttttccaa acctttagta cgggtaatta acgacaccct agaggaagaa
    13261 agaggggaaa tttagtatgc tgtgcttggg tgttttgaag tggtacggcg atgcgcggag
    13321 tccgagaaaa tctggaagag taaaaaagga gtagaaacat tttgaagcta tcagctgttt
    13381 cccgggagct ggaactccca cctgcaacat gcgggatcct gagtaaataa gcggccgcca
    13441 ccgcggtgga gctcagatct tgacaccgat tatttaaagc tgcagcatac gatatatata
    13501 catgtgtata tatgtatacc tatgaatgtc agtaagtatg tatacgaaca gtatgatact
    13561 gaagatgaca aggtaatgca tcattctata cgtgtcattc tgaacgaggc gcgctttcct
    13621 tttttctttt tgctttttct ttttttttct cttgaactcc agaaaaaaaa tataaaagag
    13681 atggaggaac gggaaaaagt tagttgtggt gataggtggc aagtggtatt ccgtaagaac
    13741 aacaagaaaa gcatttcata ttatggctga actgagcgaa caagtgcaaa atttaagcat
    13801 caacgacaac aacgagaatg gttatgttcc tcctcactta agaggaaaac caagaagtgc
    13861 cagaaataac agtagcaact acaataacaa caacggcggc tacaacggtg gccgtggcgg
    13921 tggcagcttc tttagcaaca accgtcgtgg tggttacggc aacggtggtt tcttcggtgg
    13981 aaacaacggt ggcagcggcc ggccatttaa atgtttaaac ggccacgctg gccccctata
    14041 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
    14101 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
    14161 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc
    14221 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    14281 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    14341 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    14401 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    14461 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    14521 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    14581 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    14641 gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat
    14701 ctgtgcggta tttcacaccg catagggtaa taactgatat aattaaattg aagctctaat
    14761 ttgtgagttt agtattagga tctctaccct tggcgaaaag tcctctgcca acaatgatga
    14821 tatctgatcc accacttaca acttcgtcga gggttctgta ctgctgaccc aatatgcgta
    14881 tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg
    14941 aatcaaaaaa atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat
    15001 tgaaaaggtg gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    15061 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
    15121 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
    15181 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
    15241 catgataata atggtttctt agacgtcacg cgccgaggac gtcaggtggc acttttcggg
    15301 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    15361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    15421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    15481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    15541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    15601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
    15661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    15721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    15781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    15841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    15901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    15961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    16021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    16081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    16141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    16201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    16261 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    16321 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    16381 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    16441 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    16501 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    16561 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    16621 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    16681 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    16741 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    16801 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    16861 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    16921 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    16981 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    17041 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    17101 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    17161 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    17221 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    17281 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    17341 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    17401 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcggaatta
    17461 accctcacta aagggaacaa aagctg
//
