LOCUS       sequence-340565-        7493 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses BMPR-2 lacking the extracellular domain in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Aihara S, Fujimoto S, Sakaguchi R, Imai T
  TITLE     BMPR-2 gates activity-dependent stabilization of primary dendrites 
            during mitral cell remodeling.
  JOURNAL   Cell Rep. 2021 Jun 22;35(12):109276. doi: 
            10.1016/j.celrep.2021.109276.
  PUBMED    34161760
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-22"; volume: "35(12):109276. doi"; pages: " 
            10.1016/j.celrep.2021.109276"
COMMENT     Sequence Label: pCAG-Bmpr2-Delta-EC
FEATURES             Location/Qualifiers
     source          1..7493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(4515..4534)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4580..4635
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4634..4653)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4996..5012)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4996..5012)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5009..5031)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5020..5036
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5044..5050,5051..5068,5069..5074))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5089..5110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5169..5364
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5215..5350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5370..5504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5407..5426)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5461..5480
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5572..5589)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5743..6331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5823..5842)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6502..7293,7294..7362))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7125..7144
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7363..7467)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggsgcgc gccrggsggg gsggggsggg gsgrggggsg
      541 gggsggggsg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgccaccat
     1741 gacttcctcg ctgcatcggc ccataatcat tgctttggca tcagtttctg tgttagctgt
     1801 tttgatagtc gccttatgtt ttggatacag aatgttgaca ggagaccgga aacagggtct
     1861 tcacagcatg aacatgatgg aggcggcagc agcagagccc tcccttgacc tggataacct
     1921 gaagctgctg gagctgattg gacggggtcg atacggagca gtatataaag gttccttgga
     1981 tgagcgtcca gttgctgtaa aagtattttc ttttgcaaac cgtcagaatt ttataaatga
     2041 aaaaaacatt tacagagtgc ctttgatgga acatgacaac attgctcgct tcatagttgg
     2101 agacgagagg ctcactgcag acggccgcat ggagtatttg cttgtgatgg agtattatcc
     2161 caatggatct ctgtgcaaat atctgagtct ccacacaagt gattgggtaa gctcttgccg
     2221 tctggctcat tctgtgacta gaggactggc ttatcttcac acagaattac cacgaggaga
     2281 tcattataaa cccgcaatct cccaccgaga tttaaacagc aggaatgtcc tggtaaagaa
     2341 tgacggcgcg tgtgttatca gtgactttgg tttatccatg aggctaactg gaaatcggct
     2401 ggtgcgccca ggggaagaag ataatgcggc tataagtgag gttggcacaa ttcgctatat
     2461 ggcaccagaa gtgctagaag gagctgtgaa cctgagggac tgtgagtcag ctctgaagca
     2521 agtggacatg tatgcgcttg gactcatcta ctgggaggtg tttatgaggt gtacagacct
     2581 cttcccaggt gaatctgtac cagattacca gatggctttt cagacagaag ttggaaacca
     2641 tcccacattt gaggatatgc aggttcttgt gtccagagag aagcagagac ccaagttccc
     2701 agaagcctgg aaagaaaata gcctggcagt gaggtcactc aaggaaacaa ttgaagactg
     2761 ctgggaccag gatgcagagg ctcggctcac tgcacagtgt gctgaggaga ggatggctga
     2821 actcatgatg atatgggaga gaaacaagtc tgtgagccca acggtcaacc caatgtcaac
     2881 tgctatgcag aatgaacgca acctgtcaca taataggcgt gtgccaaaaa tcgggcctta
     2941 cccagattat tcctcttcct catatattga agactctatc catcatactg acagcattgt
     3001 gaagaatatt tcctctgagc attcgatgtc cagcacacca ttgacaatag gagaaaagaa
     3061 tcgaaattca attaattatg aacgacagca agcacaagct cgaatcccta gcccagaaac
     3121 aagcgtcaca agcctgtcca caaacacaac caccacaaac accaccggcc tcactccaag
     3181 tactggcatg accactatat ctgagatgcc atacccagat gagacacatt tgcacgccac
     3241 aaatgttgca cagtcaatcg ggccaacccc tgtctgctta cagctgacag aagaagactt
     3301 ggagactaat aagctagatc caaaagaagt tgataagaac ctcaaggaaa gctctgatga
     3361 gaatctcatg gagcattctc tgaagcagtt cagtgggcca gacccattga gcagtaccag
     3421 ttctagcttg ctttatccac tcataaagct cgcagtggaa gtgactggac aacaggactt
     3481 cacacaggct gcaaatgggc aagcatgttt aattcctgat gttccacctg ctcagatcta
     3541 tcctctccct aagcaacaga accttcctaa gagacctact agtttgcctt tgaacaccaa
     3601 aaattcaaca aaagaacccc ggctaaaatt tggcaacaag cacaaatcaa acttgaaaca
     3661 agtagaaact ggagttgcca agatgaatac aatcaatgca gcagagcctc atgtggtgac
     3721 agtaactatg aatggtgtgg caggtagaag ccacaatgtt aattcccatg ctgccacaac
     3781 ccagtatgcc aatggcgcag tgccagctgg ccaggcagcc aacatagtgg cacataggtc
     3841 ccaagaaatg ctgcagaatc aatttattgg tgaggatacc aggctgaata tcaattccag
     3901 tcctgatgag catgaacctt tactgagacg agagcaacag gctggccatg atgaaggggt
     3961 tctggatcgt ttggtagata ggagggaacg gccattagaa ggtggccgaa caaattccaa
     4021 taacaacaac agcaatccat gttcagaaca agatatcctt acacaaggtg ttacaagcac
     4081 agctgcagat cctgggccat caaagcccag aagagcacag aggcccaatt ctctggatct
     4141 ttcagccaca aatatcctgg atggcagcag tatacagata ggtgagtcaa cacaagatgg
     4201 caaatcagga tcaggtgaaa agatcaagag acgtgtgaaa actccatact ctcttaagcg
     4261 gtggcgcccg tccacctggg tcatctccac cgagccgctg gactgtgagg tcaacaacaa
     4321 tggcagtgac agggcagtcc attctaaatc tagcactgct gtgtaccttg cagagggagg
     4381 cactgccacg accacagtgt ctaaagatat aggaatgaat tgtctgtgag cggccgcact
     4441 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac
     4501 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
     4561 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     4621 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     4681 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
     4741 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     4801 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     4861 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     4921 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
     4981 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5041 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     5101 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5161 gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5221 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5281 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     5341 tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca
     5401 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5461 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat
     5521 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5581 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5641 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5701 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5761 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5821 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5881 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5941 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6001 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6061 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6121 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6181 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6241 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6301 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6361 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6421 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6481 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6541 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6601 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6661 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6721 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6781 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6841 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6901 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6961 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7021 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7081 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7141 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7201 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7261 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7321 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7381 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7441 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctg
//
