LOCUS       sequence-341502-        6891 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) 
            for pseudotyping of retroviral or lentiviral vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Mirow M, Schwarze LI, Fehse B, Riecken K
  TITLE     Efficient Pseudotyping of Different Retroviral Vectors Using a 
            Novel, Codon-Optimized Gene for Chimeric GALV Envelope.
  JOURNAL   Viruses. 2021 Jul 27;13(8). pii: v13081471. doi: 10.3390/v13081471.
  PUBMED    34452336
REFERENCE   2  (bases 1 to 6891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Viruses."; 
            date: "2021-07-27"; volume: "13(8). pii: v13081471. doi"; pages: " 
            10.3390/v13081471"
COMMENT     Sequence Label: phCMV-coGALVenv-C4070A
FEATURES             Location/Qualifiers
     source          1..6891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        83..462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        463..666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     617..637
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     663..687
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          778..1350
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1417..1426
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3582..3601)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3647..3702
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3701..3720)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4060..4076)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4060..4076)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4073..4095)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4084..4100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4108..4114,4115..4132,4133..4138))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4153..4174
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4291..4308)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4462..5050)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4542..4561)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5221..6012,6013..6081))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5844..5863
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6082..6186)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6212..6667)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6349..6370)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6561..6580
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6794..6816
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6808..6825
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6809..6825
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6835..6854
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6835..6853
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca
       61 tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
      121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      721 catagaagac accgggaccg atccagcctc cggtcgaccg atcctgagaa cttcagggtg
      781 agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga
      841 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga
      901 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt
      961 attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt
     1021 tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt
     1081 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata
     1141 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
     1201 attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga
     1261 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
     1321 catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg
     1381 tctcatcatt ttggcaaaga attcctcgac ggatccgcca ccatggtttt gctgcctggc
     1441 tctatgctgc tgaccagcaa cctgcaccac ctgagacacc agatgagccc tggcagctgg
     1501 aagcggctga tcatcctgct gagctgtgtg tttggcggcg gaggcacaag cctgcagaac
     1561 aagaatcccc accagcctat gacactgacc tggcaggtcc tgtctcagac cggggatgtc
     1621 gtgtgggata caaaggctgt gcagcctcct tggacctggt ggcctacact gaagcctgat
     1681 gtgtgtgccc tggccgcctc tctggaatct tgggatattc ctggcaccga cgtgtccagc
     1741 agcaagagag tcagacctcc tgacagcgat tacaccgccg cctacaagca gatcacatgg
     1801 ggcgccatcg gctgcagcta ccctagagcc agaaccagaa tggccagcag caccttctac
     1861 gtgtgcccca gagatggcag aaccctgagc gaggctagaa gatgtggcgg cctggaaagc
     1921 ctgtactgca aagagtggga ctgcgagaca accggcacag gctactggct gagcaagagc
     1981 agcaaggacc tgatcaccgt gaagtgggac cagaacagcg agtggaccca gaaattccag
     2041 cagtgccacc agacaggctg gtgcaaccct ctgaagatcg acttcaccga caagggcaag
     2101 ctgtccaagg attggatcac cggcaagacc tggggcctgc gcttctatgt gtctggacat
     2161 cctggcgtgc agttcaccat ccggctgaag atcaccaaca tgcccgctgt ggctgtgggc
     2221 cctgatctgg tgcttgttga acagggccct cctagaacct ctctggcttt gcctcctcca
     2281 ctgcctccta gagaagcccc tccacctagc ctgcctgaca gcaattctac agccctggcc
     2341 accagcgctc agacccctac agtgcggaaa accatcgtga ccctgaacac ccctcctcca
     2401 accaccggcg acagactgtt tgatctggtc cagggcgcct tcctgacact gaacgctaca
     2461 aatcctggcg ccaccgagag ctgttggctg tgtcttgcta tgggcccacc ttactacgag
     2521 gccattgcct ctagcggcga ggtggcctat agcaccgacc tggatagatg cagatggggc
     2581 acccagggaa agctgaccct gacagaagtt tctggccacg gcctgtgcat cggcaaggtg
     2641 ccctttacac accagcacct gtgcaatcag accctgagca tcaacagcag cggcgaccac
     2701 cagtacctgc tgcctagcaa tcattcttgg tgggcctgca gcaccggcct gacaccttgt
     2761 ctgagcacca gcgtgttcaa ccagaccagg gacttctgca tccaagtgca gctgatcccc
     2821 aggatctact actaccccga ggaagtgctg ctccaggcct acgacaattc tcaccctcgg
     2881 accaagcggg aagccgtgtc tctgacactg gctgtgctgc tcggactggg aatcacagcc
     2941 ggaatcggaa caggcagcac tgccctgatc aagggcccta tcgatctgca gcagggactg
     3001 acctctctgc agattgccat cgacgccgat ctgagagccc tgcaggatag cgtgtccaag
     3061 ctggaagata gcctgaccag cctgagcgaa gtcgtgctgc agaatagaag aggcctggac
     3121 ctgctgttcc tgaaagaagg cggactttgc gccgctctga aagaggaatg ctgtttctac
     3181 atcgaccact ctggcgccgt gcgggacagc atgaagaagc tgaaagagaa gctggacaag
     3241 cgccagctgg aaagacagaa gtctcagaat tggtacgaag gctggttcaa cacaagcccc
     3301 tggttcacca cactgatcag caccatcatg ggccctctga tcgtgctgct gctgatcctg
     3361 ctgtttggcc cctgcatcct gaacagactg gtgcagttcg tgaaggacag aatcagcgtc
     3421 gtgcaggccc tggtgctgac acagcagtat caccagctga agcctctgga atacgagccc
     3481 tgataaggat ccgtcgagga attcactcct caggtgcagg ctgcctatca gaaggtggtg
     3541 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     3601 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     3661 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     3721 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     3781 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     3841 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     3901 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     3961 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     4021 tggagatccc tcgacggatc ggccgcaatt cgtaatcatg tcatagctgt ttcctgtgtg
     4081 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     4141 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     4201 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     4261 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4321 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4381 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4441 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4501 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4561 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4621 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4681 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4741 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4801 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4861 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4921 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4981 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5041 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5101 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5161 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5221 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5281 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5341 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5401 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5461 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5521 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5581 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5641 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5701 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5761 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5821 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5881 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5941 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     6001 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     6061 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6121 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6181 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     6241 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     6301 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     6361 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     6421 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     6481 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     6541 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     6601 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     6661 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     6721 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     6781 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
     6841 gactcactat agggcgaatt ggagctccac cgcggtggcg gccgctctag a
//
