LOCUS       sequence-341673-       11186 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Drosophila transgene expression under QUAS/QF control, PhiC31 
            transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11186)
  TITLE     McCabe lab pBID2 plasmids 2021
REFERENCE   2  (bases 1 to 11186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBID2_QUAS>Gateway
FEATURES             Location/Qualifiers
     source          1..11186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4789..4822
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4835..5265
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5709..5833
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5858..5863,5864..5881,5882..5888)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5942..6601
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6008..6027)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6943..7248
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7127..7146
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7289..7413)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    8110..8244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8147..8166)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8201..8220
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    8246..8676
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    8710..8779
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(8998..9016)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9037..9053)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9037..9053)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9050..9072)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9061..9077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9085..9091,9092..9109,9110..9115))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9130..9151
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9268..9285)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9439..10027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9519..9538)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10198..10989,10990..11058))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10821..10840
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11059..11163)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca caccacgttt cgtagttgct ctttcgctgt ctcccacccg ctctccgcaa
     4381 cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc gcgattcggt
     4441 tcgctcaaat ggttccgagt ggttcatttc gtctcaatag aaattagtaa taaatatttg
     4501 tatgtacaat ttatttgctc caatatattt gtatatattt ccctcacagc tatatttatt
     4561 ctaatttaat attatgactt tttaaggtaa ttttttgtga cctgttcgga gtgattagcg
     4621 ttacaatttg aactgaaagt gacatccagt gtttgttcct tgtgtagatg catctcaaaa
     4681 aaatggtggg cataatagtg ttgtttatat atatcaaaaa taacaactat aataataaga
     4741 atacatttaa tttagaaaat gcttggattt cactggaact aggctagcat aacttcgtat
     4801 aatgtatgct atacgaagtt atgctagcgg atcctggcca cgtaataagt gtgcgttgaa
     4861 tttattcgca aaaacattgc atattttcgg caaagtaaaa ttttgttgca taccttatca
     4921 aaaaataagt gctgcatact ttttagagaa accaaataat tttttattgc atacccgttt
     4981 ttaataaaat acattgcata ccctctttta ataaaaaata ttgcatactt tgacgaaaca
     5041 aattttcgtt gcatacccaa taaaagatta ttatattgca tacccgtttt taataaaata
     5101 cattgcatac cctcttttaa taaaaaatat tgcatacgtt gacgaaacaa attttcgttg
     5161 catacccaat aaaagattat tatattgcat accttttctt gccataccat ttagccgatc
     5221 aattgtgctc ggcaacagta tatttgtggt gtgccaacca acaacagatc caagctggcc
     5281 gcggctcgat aagcttgtca ctgggtcagt gggtaatcgc ttatcctcgg ataaacaatt
     5341 atcctcacgg gtaatcgctt atccgctcgg gtaatcgctt atcctcgggt aatcgcttat
     5401 ccttgggtaa tcgcttatcc tcggataaac aattatcctc acgggtaatc gcttatccgc
     5461 tcgggtaatc gcttatcctc gggtaatcgc ttatcctttc acgttgggac gtcgagactc
     5521 tagcgagctc gcccggggat cgagcgcagc ggtataaaag ggcgcggggt ggctgagagc
     5581 atcagttgtg aatgaatgtt cgagccgagc agacgtgccg ctgccttcgt taatatcctt
     5641 tgaataagcc aactttgaat cacaagacgc ataccaaacg aattcgcaga tcttaattaa
     5701 ccggtaccac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
     5761 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
     5821 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
     5881 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
     5941 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     6001 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     6061 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     6121 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     6181 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     6241 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     6301 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     6361 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     6421 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     6481 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     6541 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     6601 aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     6661 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
     6721 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     6781 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     6841 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     6901 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
     6961 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     7021 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     7081 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     7141 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     7201 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     7261 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta
     7321 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     7381 cgtttctcgt tcagctttct tgtacaaagt ggtgatatcg catgcggccg cggcgcgcct
     7441 ctagaaatga atcgttttta aaataacaaa tcaattgttt tataatattc gtacgattct
     7501 ttgattatgt aataaaatgt gatcattagg aagattacga aaaatataaa aaatatgagt
     7561 tctgtgtgta taacaaatgc tgtaaacgcc acaattgtgt ttgttgcaaa taaacccatg
     7621 attatttgat taaaattgtt gttttctttg ttcatagaca atagtgtgtt ttgcctaaac
     7681 gtgtactgca taaactccat gcgagtgtat agcgagctag tggctaacgc ttgccccacc
     7741 aaagtagatt cgtcaaaatc ctcaatttca tcaccctcct ccaagtttaa catttggccg
     7801 tcggaattaa cttctaaaga tgccacataa tctaataaat gaaatagaga ttcaaacgtg
     7861 gcgtcatcgt ccgtttcgac catttccgaa aagaactcgg gcataaactc tatgatttct
     7921 ctggacgtgg tgttgtcgaa actctcaaag tacgcagtca ggaacgtgcg cgacatgtcg
     7981 tcgggaaact cgcgcggaaa catgttgttg taaccgaacg ggtcccatag cgccaaaacc
     8041 aaatctgcca gcgtcaatag aatgagcacg atgccgacaa tggagctggc ttggatagcg
     8101 attcgagtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8161 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8221 aatgtatctt atcatgtctg gatcttggcc acgtaataag tgtgcgttga atttattcgc
     8281 aaaaacattg catattttcg gcaaagtaaa attttgttgc ataccttatc aaaaaataag
     8341 tgctgcatac tttttagaga aaccaaataa ttttttattg catacccgtt tttaataaaa
     8401 tacattgcat accctctttt aataaaaaat attgcatact ttgacgaaac aaattttcgt
     8461 tgcataccca ataaaagatt attatattgc atacccgttt ttaataaaat acattgcata
     8521 ccctctttta ataaaaaata ttgcatacgt tgacgaaaca aattttcgtt gcatacccaa
     8581 taaaagatta ttatattgca taccttttct tgccatacca tttagccgat caattgtgct
     8641 cggcaacagt atatttgtgg tgtgccaacc aacaacggat ccactagtgt cgacgatgta
     8701 ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc
     8761 gtactccacc tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat
     8821 cccggcgaac gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt
     8881 cacggtgagc acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag
     8941 cgggttctcg acggtcacgg cgggcatgtc gacactagtt ctagccagct tttgttccct
     9001 ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9061 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9121 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9181 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9241 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9301 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9361 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9421 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9481 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9541 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9601 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9661 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9721 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9781 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9841 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9901 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9961 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10021 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10081 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10141 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10201 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10261 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10321 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10381 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10441 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10501 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10561 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10621 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10681 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10741 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10801 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10861 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10921 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10981 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11041 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11101 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11161 gcgcacattt ccccgaaaag tgccac
//
