LOCUS       sequence-341681-       11966 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Drosophila QF2 expression under introduced sequence control, PhiC31 
            transgenesis .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11966)
  TITLE     McCabe lab pBID2 plasmids 2021
REFERENCE   2  (bases 1 to 11966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBID2_Gateway>QF2
FEATURES             Location/Qualifiers
     source          1..11966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4791..4824
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4837..5267
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5290..5414
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5439..5444,5445..5462,5463..5469)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5523..6182
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5589..5608)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6524..6829
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6708..6727
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6870..6994)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          7182..7248
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     protein_bind    9026..9456
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    9490..9559
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(9778..9796)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9817..9833)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9817..9833)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9830..9852)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9841..9857
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9865..9871,9872..9889,9890..9895))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9910..9931
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10048..10065)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10219..10807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10299..10318)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10978..11769,11770..11838))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11601..11620
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11839..11943)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt tttttaccat tattaccatc gtgtttactg
     4081 tttattgccc cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa caagatatga
     4141 cctatagaat acaagtattt ccccttcgaa catccccaca agtagacttt ggatttgtct
     4201 tctaaccaaa agacttacac acctgcatac cttacatcaa aaactcgttt atcgctacat
     4261 aaaacaccgg gatatatttt ttatatacat acttttcaaa tcgcgcgccc tcttcataat
     4321 tcacctccac cacaccacgt ttcgtagttg ctctttcgct gtctcccacc cgctctccgc
     4381 aacacattca ccttttgttc gacgaccttg gagcgactgt cgttagttcc gcgcgattcg
     4441 gttcgctcaa atggttccga gtggttcatt tcgtctcaat agaaattagt aataaatatt
     4501 tgtatgtaca atttatttgc tccaatatat ttgtatatat ttccctcaca gctatattta
     4561 ttctaattta atattatgac tttttaaggt aattttttgt gacctgttcg gagtgattag
     4621 cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc cttgtgtaga tgcatctcaa
     4681 aaaaatggtg ggcataatag tgttgtttat atatatcaaa aataacaact ataataataa
     4741 gaatacattt aatttagaaa atgcttggat ttcactggaa ctaggctagc ataacttcgt
     4801 ataatgtatg ctatacgaag ttatgctagc ggatcctggc cacgtaataa gtgtgcgttg
     4861 aatttattcg caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat
     4921 caaaaaataa gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt
     4981 ttttaataaa atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa
     5041 caaattttcg ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa
     5101 tacattgcat accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt
     5161 tgcataccca ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga
     5221 tcaattgtgc tcggcaacag tatatttgtg gtgtgccaac caacaacaga tctgaattcg
     5281 cttaattaaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
     5341 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     5401 atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
     5461 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
     5521 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     5581 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     5641 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     5701 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
     5761 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     5821 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     5881 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     5941 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     6001 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     6061 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     6121 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     6181 aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
     6241 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
     6301 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
     6361 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
     6421 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
     6481 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
     6541 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     6601 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     6661 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     6721 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
     6781 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
     6841 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt
     6901 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     6961 acgtttctcg ttcagctttc ttgtacaaag tggtgataaa cggccggccg agctcgcccg
     7021 gggatcgagc gcagcggtat aaaagggcgc ggggtggctg agagcatcag ttgtgaatga
     7081 atgttcgagc cgagcagacg tgccgctgcc ttcgttaata tcctttgaat aagccaactt
     7141 tgaatcacaa gacgcatacc aaacggtacc agatctaaaa ggtaggttca accactgatg
     7201 cctaggcaca ccgaaacgac taaccctaat tcttatcctt tacttcaggc ggccgcgttt
     7261 aaacaactta aaaaaaaaaa tcaaaatgcc acccaagcgc aaaacgctta acgctgcggc
     7321 tgaggctaac gctcatgccg acggacacgc cgacggaaac gccgacggac acgtggccaa
     7381 tacggccgcg tcctcgaata atgcgaggtt cgctgatctc actaacatcg atactccggg
     7441 tctgggaccc acaactacga ccctgctcgt ggaaccagca cgctcaaagc gtcaacgagt
     7501 gtcccgcgca tgcgaccagt gccgtgcagc ccgagagaaa tgcgacggaa tacagcctgc
     7561 gtgtttcccg tgcgtttccc agggaaggtc ctgcacttat caggcttcgc cgaaaaagag
     7621 gggagttcaa accggttata ttcgtacgct ggagctcgcc ctcgcctgga tgtttgaaaa
     7681 tgtcgcgcgt tccgaagatg ccttgcataa cctcctcgtc cgtgacgccg gacaaggatc
     7741 agctctgctc gttggtaaag attcgccggc tgccgagcga ctccatgccc gttgggctac
     7801 tagccgtgtc aataagagca ttacccgcct cctccgtcag ttggagctcc ctcctaccgc
     7861 cacggctacg gcctcgataa tgccgcacgt gatggagcag cctctcagta ccagcattaa
     7921 ccccgtcaac gaccgcttca acggtattcc caaccccact ccgtataact ccgatgcagc
     7981 tctcgatgct atcactcaga ccaacgatta tggaagcgta aatacacatg gtatcctctc
     8041 tacttacccg ccaccggcta cgcaccttaa tgaagcttcc gtcgctctcg ctcccggtgg
     8101 cgcccccccc cgaccgcctc ctccgtatgt tgacagcacg accaatcacc cgccgtacca
     8161 ctcgaatctg gttccaatgg cgaactttgg ttactcgacc gttgattacg atgccatggt
     8221 tgacgatttg gctagcattg aatacacgga cgctgtggat gtcgacccac agtttatgac
     8281 caatctggga ttcgttcctg gatgtaactt ctccgacatt aatacatacg aacagtgacc
     8341 tgcaggaatg aatcgttttt aaaataacaa atcaattgtt ttataatatt cgtacgattc
     8401 tttgattatg taataaaatg tgatcattag gaagattacg aaaaatataa aaaatatgag
     8461 ttctgtgtgt ataacaaatg ctgtaaacgc cacaattgtg tttgttgcaa ataaacccat
     8521 gattatttga ttaaaattgt tgttttcttt gttcatagac aatagtgtgt tttgcctaaa
     8581 cgtgtactgc ataaactcca tgcgagtgta tagcgagcta gtggctaacg cttgccccac
     8641 caaagtagat tcgtcaaaat cctcaatttc atcaccctcc tccaagttta acatttggcc
     8701 gtcggaatta acttctaaag atgccacata atctaataaa tgaaatagag attcaaacgt
     8761 ggcgtcatcg tccgtttcga ccatttccga aaagaactcg ggcataaact ctatgatttc
     8821 tctggacgtg gtgttgtcga aactctcaaa gtacgcagtc aggaacgtgc gcgacatgtc
     8881 gtcgggaaac tcgcgcggaa acatgttgtt gtaaccgaac gggtcccata gcgccaaaac
     8941 caaatctgcc agcgtcaata gaatgagcac gatgccgaca atggagctgg cttggatagc
     9001 gattcgagtt aactctagaa gatcttggcc acgtaataag tgtgcgttga atttattcgc
     9061 aaaaacattg catattttcg gcaaagtaaa attttgttgc ataccttatc aaaaaataag
     9121 tgctgcatac tttttagaga aaccaaataa ttttttattg catacccgtt tttaataaaa
     9181 tacattgcat accctctttt aataaaaaat attgcatact ttgacgaaac aaattttcgt
     9241 tgcataccca ataaaagatt attatattgc atacccgttt ttaataaaat acattgcata
     9301 ccctctttta ataaaaaata ttgcatacgt tgacgaaaca aattttcgtt gcatacccaa
     9361 taaaagatta ttatattgca taccttttct tgccatacca tttagccgat caattgtgct
     9421 cggcaacagt atatttgtgg tgtgccaacc aacaacggat ccactagtgt cgacgatgta
     9481 ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc
     9541 gtactccacc tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat
     9601 cccggcgaac gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt
     9661 cacggtgagc acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag
     9721 cgggttctcg acggtcacgg cgggcatgtc gacactagtt ctagccagct tttgttccct
     9781 ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9841 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9901 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9961 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    10021 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    10081 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    10141 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    10201 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    10261 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10321 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10381 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10441 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10501 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10561 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10621 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    10681 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10741 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10801 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10861 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10921 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10981 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    11041 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    11101 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    11161 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    11221 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    11281 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    11341 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11401 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11461 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    11521 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    11581 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    11641 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    11701 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    11761 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11821 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11881 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11941 gcgcacattt ccccgaaaag tgccac
//
