LOCUS       sequence-341733-       27118 bp    DNA     circular UNA 15-APR-2025
DEFINITION  Drosophila Gal4 expression under introduced sequence control, PhiC31
            transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 27118)
  TITLE     McCabe lab pBID2 plasmids 2021
REFERENCE   2  (bases 1 to 27118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBID2_Gateway>Gal4
FEATURES             Location/Qualifiers
     source          1..27118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3201..3332,
                     3403..4017)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4792..4825
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4838..5268
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5291..5415
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5440..5445,5446..5463,5464..5470)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5524..6183
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             6525..6830
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(6871..6995)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          7183..7249
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     protein_bind    10619..11049
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    11083..11152
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(11371..11389)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11410..11426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11434..11450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11458..11464,11465..11482,11483..11488))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11503..11524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(11812..12400)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(12571..13362,13363..13431))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13432..13536)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13562..14017)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     14159..14175
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        14182..14200
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(14704..14775,15177..15450,15525..16179,16241..16556,
                     16760..16891,16962..17576)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    18351..18384
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    18397..18827
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    18850..18974
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(18999..19004,19005..19022,19023..19029)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             19083..19742
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             20084..20389
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(20430..20554)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          20742..20808
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     protein_bind    24178..24608
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    24642..24711
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(24930..24948)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(24969..24985)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    24993..25009
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(25017..25023,25024..25041,25042..25047))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    25062..25083
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(25371..25959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(26130..26921,26922..26990))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(26991..27095)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggctctaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcctt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agtattacat aacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgccattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatctggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagata gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaagat aaataaattt catttaactc gcgaatatta atgagatgcg
     3181 agtaacattt taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac
     3241 aactcacgca agtgggtgtg atgaatatca acggagccat cttcctcttc ctgaccaaca
     3301 tgacctttca aaacgtcttt gccacgataa atgtaagtca tgtttagaat acatttgcat
     3361 ttcaataatt tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg
     3421 ccagttttta tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc
     3481 aaaacgattg ccgaattgcc gctttttctc acagtgccac tggtcttcac ggcgattgcc
     3541 tatccgatga tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc
     3601 actctggtgg ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg
     3661 acctcgatgg cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc
     3721 ttcttcttga actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg
     3781 ttccgttacg ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa
     3841 attagctgca catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg
     3901 cttaacttct ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg
     3961 agcttccggg tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg
     4021 acatatatcc gaaataactg cttgtttttt ttttttacca ttattaccat cgtgtttact
     4081 gtttattgcc ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg
     4141 acctatagaa tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc
     4201 ttctaaccaa aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca
     4261 taaaacaccg ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa
     4321 ttcacctcca ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg
     4381 caacacattc accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc
     4441 ggttcgctca aatggttccg agtggttcat ttcgtctcaa tagaaattag taataaatat
     4501 ttgtatgtac aatttatttg ctccaatata tttgtatata tttccctcac agctatattt
     4561 attctaattt aatattatga ctttttaagg taattttttg tgacctgttc ggagtgatta
     4621 gcgttacaat ttgaactgaa agtgacatcc agtgtttgtt ccttgtgtag atgcatctca
     4681 aaaaaatggt gggcataata gtgttgttta tatatatcaa aaataacaac tataataata
     4741 agaatacatt taatttagaa aatgcttgga tttcactgga actaggctag cataacttcg
     4801 tataatgtat gctatacgaa gttatgctag cggatcctgg ccacgtaata agtgtgcgtt
     4861 gaatttattc gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta
     4921 tcaaaaaata agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg
     4981 tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa
     5041 acaaattttc gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa
     5101 atacattgca taccctcttt taataaaaaa tattgcatac gttgacgaaa caaattttcg
     5161 ttgcataccc aataaaagat tattatattg catacctttt cttgccatac catttagccg
     5221 atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa ccaacaacag atctgaattc
     5281 gcttaattaa acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     5341 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     5401 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
     5461 tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct
     5521 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     5581 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     5641 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     5701 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     5761 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     5821 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     5881 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     5941 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     6001 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     6061 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     6121 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     6181 taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     6241 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg
     6301 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     6361 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     6421 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     6481 tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt
     6541 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     6601 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     6661 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     6721 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     6781 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     6841 cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag
     6901 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     6961 tacgtttctc gttcagcttt cttgtacaaa gtggtgataa acggccggcc gagctcgccc
     7021 ggggatcgag cgcagcggta taaaagggcg cggggtggct gagagcatca gttgtgaatg
     7081 aatgttcgag ccgagcagac gtgccgctgc cttcgttaat atcctttgaa taagccaact
     7141 ttgaatcaca agacgcatac caaacggtac cagatctaaa aggtaggttc aaccactgat
     7201 gcctaggcac accgaaacga ctaaccctaa ttcttatcct ttacttcagg cggccgcgtt
     7261 aaacaactta aaaaaaaaaa tcaaaatgaa gctgctgagt agtattgaac aagcgtgtga
     7321 catatgccga cttaagaagc tgaagtgcag caaggaaaag ccgaagtgcg ccaaatgtct
     7381 taagaacaat tgggagtgcc ggtactcgcc aaagacgaag cgaagccctt tgacacgcgc
     7441 tcatttgact gaagtggaga gtcgcctgga gcgccttgag cagttgttcc tcctcatttt
     7501 tccccgtgaa gacctcgaca tgatactgaa gatggatagt ctccaagata ttaaggccct
     7561 gctcactggc ctgtttgttc aggataacgt caacaaggat gccgtgacgg accgccttgc
     7621 ctcggtggag acagatatgc cacttacctt gcggcagcat cgcatctcgg ctaccagcag
     7681 tagtgaagaa agttccaata agggtcaacg gcaactgacg gtatcgatcg acagtgcggc
     7741 ccaccacgac aacagcacca ttccactgga cttcatgcct cgtgacgcac tgcatggatt
     7801 tgactggagc gaggaggatg atatgagcga cggactccca ttcctgaaga ccgaccccaa
     7861 taacaatggt ttctttggcg acggcagcct gctgtgtatc ctgcgaagta ttggttttaa
     7921 gccggaaaat tacacgaaca gtaatgtcaa tcgtcttccc acaatgatca ccgaccgtta
     7981 cacgctggcc agtcgcagta ctacaagccg cctgctgcaa agctatctta acaacttcca
     8041 cccctactgc cccatagtgc atagtcctac tctcatgatg ctttacaaca atcagattga
     8101 gatagcatcg aaggaccagt ggcagatcct cttcaactgc attctcgcca tcggcgcttg
     8161 gtgtattgaa ggcgagagca cagatatcga cgtgttttac tatcaaaacg ccaagtcgca
     8221 cctcacctcg aaggtcttcg aaagtggaag cattatcctc gtgacagccc tccatctgct
     8281 gagtcgttat acccagtggc gtcagaaaac taatacatcc tacaactttc attcgttcag
     8341 cattcggatg gcgattagcc tgggacttaa tcgtgacctc ccaagtagct tcagcgattc
     8401 gtcgatcctg gaacagcgcc ggaggatttg gtggagtgtg tacagctggg agatccagct
     8461 gtccctcttg tacggacgca gcattcagct gagtcagaac acgatttcct tcccgtccag
     8521 cgtggacgac gttcaacgga caactacggg cccgaccatc taccacggta tcattgagac
     8581 ggcccgcttg cttcaagtgt ttacgaagat ttatgagctg gataagacag tgaccgcgga
     8641 gaagagtccg atctgcgcca agaaatgcct gatgatctgc aatgagatag aggaggtgag
     8701 ccgccaggca cccaagttcc tgcagatgga cataagcact acggcgctga ctaatctcct
     8761 caaagaacac ccctggctga gtttcacccg cttcgagctg aagtggaaac agctgagtct
     8821 gattatatac gttttgcggg actttttcac aaattttacg cagaagaagt cgcagcttga
     8881 gcaagatcag aacgaccatc agtcctacga ggtaaagagg tgcagcataa tgctctccga
     8941 cgcagcccag cgcaccgtga tgtcggtgtc gtcctatatg gacaaccata atgtgacccc
     9001 ttatttcgcc tggaattgct cctactattt gttcaacgcc gtgttggtcc caatcaagac
     9061 tcttctgagc aactccaaat cgaacgccga gaataacgag acagcacagc tcttgcagca
     9121 aattaacacc gtgttgatgc tgttgaagaa actcgctaca ttcaagattc agacttgcga
     9181 aaagtacatc caggtgttgg aagaggtgtg cgccccattc cttctttcgc aatgcgcaat
     9241 tcccctcccg cacataagtt acaataattc caacggctcc gctatcaaga atatcgtcgg
     9301 ctccgctaca atcgcgcagt atcccacgct gcctgaggaa aatgttaaca acatatcggt
     9361 gaagtatgta agtccaggca gcgtcggccc gagccctgtg ccgctgaaaa gcggagcctc
     9421 cttcagcgat ctggtcaagc tgctcagcaa ccgcccacca tcccgcaact cccctgtcac
     9481 tataccccga tcgaccccgt cgcatcgtag cgttacccca ttcctgggac aacagcagca
     9541 actgcagagt ctggtgccac ttacgccctc ggcattgttt ggcggagcca acttcaacca
     9601 gagtggtaac attgcggaca gctccttgag cttcacgttt accaacagct ccaacggtcc
     9661 aaacttgatc acgactcaga caaactccca ggcattgtcc cagcccattg ccagctcgaa
     9721 tgtgcatgat aactttatga ataatgaaat tactgcctcc aagattgacg acggcaacaa
     9781 cagcaagccc ctttccccag gctggactga ccagacggcc tacaatgctt tcggtatcac
     9841 cactggcatg ttcaacacca caacgatgga cgacgtatat aactatcttt ttgatgatga
     9901 ggatactcca ccgaacccaa agaaggagta gcctgcagga atgaatcgtt tttaaaataa
     9961 caaatcaatt gttttataat attcgtacga ttctttgatt atgtaataaa atgtgatcat
    10021 taggaagatt acgaaaaata taaaaaatat gagttctgtg tgtataacaa atgctgtaaa
    10081 cgccacaatt gtgtttgttg caaataaacc catgattatt tgattaaaat tgttgttttc
    10141 tttgttcata gacaatagtg tgttttgcct aaacgtgtac tgcataaact ccatgcgagt
    10201 gtatagcgag ctagtggcta acgcttgccc caccaaagta gattcgtcaa aatcctcaat
    10261 ttcatcaccc tcctccaagt ttaacatttg gccgtcggaa ttaacttcta aagatgccac
    10321 ataatctaat aaatgaaata gagattcaaa cgtggcgtca tcgtccgttt cgaccatttc
    10381 cgaaaagaac tcgggcataa actctatgat ttctctggac gtggtgttgt cgaaactctc
    10441 aaagtacgca gtcaggaacg tgcgcgacat gtcgtcggga aactcgcgcg gaaacatgtt
    10501 gttgtaaccg aacgggtccc atagcgccaa aaccaaatct gccagcgtca atagaatgag
    10561 cacgatgccg acaatggagc tggcttggat agcgattcga gttaactcta gaagatcttg
    10621 gccacgtaat aagtgtgcgt tgaatttatt cgcaaaaaca ttgcatattt tcggcaaagt
    10681 aaaattttgt tgcatacctt atcaaaaaat aagtgctgca tactttttag agaaaccaaa
    10741 taatttttta ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa
    10801 aatattgcat actttgacga aacaaatttt cgttgcatac ccaataaaag attattatat
    10861 tgcatacccg tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata
    10921 cgttgacgaa acaaattttc gttgcatacc caataaaaga ttattatatt gcataccttt
    10981 tcttgccata ccatttagcc gatcaattgt gctcggcaac agtatatttg tggtgtgcca
    11041 accaacaacg gatccactag tgtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg
    11101 gtgccagggc gtgcccttgg gctccccggg cgcgtactcc acctcaccca tctggtccat
    11161 catgatgaac gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa
    11221 gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc
    11281 gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc tcgacggtca cggcgggcat
    11341 gtcgacacta gttctagcca gcttttgttc cctttagtga gggttaattt cgagcttggc
    11401 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    11461 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    11521 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    11581 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    11641 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    11701 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    11761 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    11821 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    11881 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    11941 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    12001 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    12061 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    12121 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    12181 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    12241 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    12301 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    12361 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    12421 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    12481 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    12541 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    12601 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    12661 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    12721 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    12781 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    12841 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    12901 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    12961 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    13021 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    13081 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    13141 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    13201 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    13261 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    13321 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    13381 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    13441 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    13501 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    13561 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
    13621 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
    13681 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
    13741 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
    13801 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
    13861 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
    13921 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
    13981 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
    14041 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
    14101 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
    14161 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattggg tacgtaccgg
    14221 gcccctagta tgtatgtaag ttaataaaac ccatttttgc ggaaagtaga taaaaaaaac
    14281 attttttttt tttactgcac tggatatcat tgaacttatc tgatcagttt taaatttact
    14341 tcgatccaag ggtatttgat gtaccaggtt ctttcgatta cctctcactc aaaatgacat
    14401 tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc gccgtctctt
    14461 tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacgtag cgtcaatgtc
    14521 cgccttcagt tgcattttgt cagcggtttc gtgacgaagc tccaagcggt ttacgccatc
    14581 aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt ttgttttttg
    14641 agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg
    14701 gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca cccatctgcc
    14761 gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact tgatctatag
    14821 gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc
    14881 aaaccgcaat tgcacaaaca aatagtgaca cgaaacagat tattctggta gctgttctcg
    14941 ctatataaga caatttttga gatcatatca tgatcaagac atctaaaggc attcattttc
    15001 gactatattc ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc
    15061 acaaaaaata aataaaagta taaacctact tcgtaggata cttcggggta ctttttgttc
    15121 ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca ttgcagggtg
    15181 acagcggagc ggcttcgcag agctgcatta accagggctt cgggcaggcc aaaaactacg
    15241 gcacgctccg gccacccagt ccgccggagg actccggttc agggagcggc caactagccg
    15301 agaacctcac ctatgcctgg cacaatatgg acatctttgg ggcggtcaat cagccgggct
    15361 ccggatggcg gcagctggtc aaccggacac gcggactatt ctgcaacgag cgacacatac
    15421 cggcgcccag gaaacatttg ctcaagaacg gtgagtttct attcgcagtc ggctgatctg
    15481 tgtgaaatct taataaaggg tccaattacc aatttgaaac tcagtttgcg gcgtggccta
    15541 tccgggcgaa cttttggccg tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa
    15601 tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg ccatccggga tgcgactgct
    15661 caatggccaa cctgtggacg ccaaggagat gcaggccagg tgcgcctatg tccagcagga
    15721 tgacctcttt atcggctccc taacggccag ggaacacctg attttccaag ccatggtgcg
    15781 gatgccacga catctgacct atcggcagcg agtggcccgc gtggatcagg tgatccagga
    15841 gctttcgctc agcaaatgtc agcacacgat catcggtgtg cccggcaggg tgaaaggtct
    15901 gtccggcgga gaaaggaagc gtctggcatt cgcctccgag gctctaaccg atccgccgct
    15961 tctgatctgc gatgagccca cctccggact ggactccttt accgcccaca gcgtcgtcca
    16021 ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc atcagccgtc
    16081 ttccgagctg tttgagctct ttgacaagat ccttctgatg gccgagggca gggtagcttt
    16141 cttgggcact cccagcgaag ccgtcgactt cttttcctag tgagttcgat gtgtttatta
    16201 agggtatcta gtattacata acatctcaac tcctatccag cgtgggtgcc cagtgtccta
    16261 ccaactacaa tccggcggac ttttacgtac aggtgttggc cgttgtgccc ggacgggaga
    16321 tcgagtcccg tgatcggatc gccaagatat gcgacaattt tgccattagc aaagtagccc
    16381 gggatatgga gcagttgttg gccaccaaaa atctggagaa gccactggag cagccggaga
    16441 atgggtacac ctacaaggcc acctggttca tgcagttccg ggcggtcctg tggcgatcct
    16501 ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg acttattcag acaacggtga
    16561 gtggttccag tggaaacaaa tgatataacg cttacaattc ttggaaacaa attcgctaga
    16621 ttttagatag aattgcctga ttccacaccc ttcttagttt ttttcaatga gatgtatagt
    16681 ttatagtttt gcagaagata aataaatttc atttaactcg cgaatattaa tgagatgcga
    16741 gtaacatttt aatttgcaga tggttgccat cttgattggc ctcatctttt tgggccaaca
    16801 actcacgcaa gtgggtgtga tgaatatcaa cggagccatc ttcctcttcc tgaccaacat
    16861 gacctttcaa aacgtctttg ccacgataaa tgtaagtcat gtttagaata catttgcatt
    16921 tcaataattt actaactttc taatgaatcg attcgattta ggtgttcacc tcagagctgc
    16981 cagtttttat gagggaggcc cgaagtcgac tttatcgctg tgacacatac tttctgggca
    17041 aaacgattgc cgaattgccg ctttttctca cagtgccact ggtcttcacg gcgattgcct
    17101 atccgatgat cggactgcgg gccggagtgc tgcacttctt caactgcctg gcgctggtca
    17161 ctctggtggc caatgtgtca acgtccttcg gatatctaat atcctgcgcc agctcctcga
    17221 cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc attcctgctc tttggcggct
    17281 tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac ctctcatggt
    17341 tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag ccgggcgaaa
    17401 ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc ctggagacgc
    17461 ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggccatt ctcatcgtga
    17521 gcttccgggt gctcgcatat ctggctctaa gacttcgggc ccgacgcaag gagtagccga
    17581 catatatccg aaataactgc ttgttttttt tttttaccat tattaccatc gtgtttactg
    17641 tttattgccc cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa caagatatga
    17701 cctatagaat acaagtattt ccccttcgaa catccccaca agtagacttt ggatttgtct
    17761 tctaaccaaa agacttacac acctgcatac cttacatcaa aaactcgttt atcgctacat
    17821 aaaacaccgg gatatatttt ttatatacat acttttcaaa tcgcgcgccc tcttcataat
    17881 tcacctccac cacaccacgt ttcgtagttg ctctttcgct gtctcccacc cgctctccgc
    17941 aacacattca ccttttgttc gacgaccttg gagcgactgt cgttagttcc gcgcgattcg
    18001 gttcgctcaa atggttccga gtggttcatt tcgtctcaat agaaattagt aataaatatt
    18061 tgtatgtaca atttatttgc tccaatatat ttgtatatat ttccctcaca gctatattta
    18121 ttctaattta atattatgac tttttaaggt aattttttgt gacctgttcg gagtgattag
    18181 cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc cttgtgtaga tgcatctcaa
    18241 aaaaatggtg ggcataatag tgttgtttat atatatcaaa aataacaact ataataataa
    18301 gaatacattt aatttagaaa atgcttggat ttcactggaa ctaggctagc ataacttcgt
    18361 ataatgtatg ctatacgaag ttatgctagc ggatcctggc cacgtaataa gtgtgcgttg
    18421 aatttattcg caaaaacatt gcatattttc ggcaaagtaa aattttgttg cataccttat
    18481 caaaaaataa gtgctgcata ctttttagag aaaccaaata attttttatt gcatacccgt
    18541 ttttaataaa atacattgca taccctcttt taataaaaaa tattgcatac tttgacgaaa
    18601 caaattttcg ttgcataccc aataaaagat tattatattg catacccgtt tttaataaaa
    18661 tacattgcat accctctttt aataaaaaat attgcatacg ttgacgaaac aaattttcgt
    18721 tgcataccca ataaaagatt attatattgc ataccttttc ttgccatacc atttagccga
    18781 tcaattgtgc tcggcaacag tatatttgtg gtgtgccaac caacaacaga tctgaattcg
    18841 cttaattaaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
    18901 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
    18961 atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
    19021 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
    19081 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
    19141 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
    19201 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
    19261 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
    19321 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
    19381 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
    19441 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
    19501 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
    19561 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
    19621 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
    19681 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
    19741 aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
    19801 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
    19861 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
    19921 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
    19981 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
    20041 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
    20101 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
    20161 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
    20221 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
    20281 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
    20341 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
    20401 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt
    20461 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
    20521 acgtttctcg ttcagctttc ttgtacaaag tggtgataaa cggccggccg agctcgcccg
    20581 gggatcgagc gcagcggtat aaaagggcgc ggggtggctg agagcatcag ttgtgaatga
    20641 atgttcgagc cgagcagacg tgccgctgcc ttcgttaata tcctttgaat aagccaactt
    20701 tgaatcacaa gacgcatacc aaacggtacc agatctaaaa ggtaggttca accactgatg
    20761 cctaggcaca ccgaaacgac taaccctaat tcttatcctt tacttcaggc ggccgcgtta
    20821 aacaacttaa aaaaaaaaat caaaatgaag ctgctgagta gtattgaaca agcgtgtgac
    20881 atatgccgac ttaagaagct gaagtgcagc aaggaaaagc cgaagtgcgc caaatgtctt
    20941 aagaacaatt gggagtgccg gtactcgcca aagacgaagc gaagcccttt gacacgcgct
    21001 catttgactg aagtggagag tcgcctggag cgccttgagc agttgttcct cctcattttt
    21061 ccccgtgaag acctcgacat gatactgaag atggatagtc tccaagatat taaggccctg
    21121 ctcactggcc tgtttgttca ggataacgtc aacaaggatg ccgtgacgga ccgccttgcc
    21181 tcggtggaga cagatatgcc acttaccttg cggcagcatc gcatctcggc taccagcagt
    21241 agtgaagaaa gttccaataa gggtcaacgg caactgacgg tatcgatcga cagtgcggcc
    21301 caccacgaca acagcaccat tccactggac ttcatgcctc gtgacgcact gcatggattt
    21361 gactggagcg aggaggatga tatgagcgac ggactcccat tcctgaagac cgaccccaat
    21421 aacaatggtt tctttggcga cggcagcctg ctgtgtatcc tgcgaagtat tggttttaag
    21481 ccggaaaatt acacgaacag taatgtcaat cgtcttccca caatgatcac cgaccgttac
    21541 acgctggcca gtcgcagtac tacaagccgc ctgctgcaaa gctatcttaa caacttccac
    21601 ccctactgcc ccatagtgca tagtcctact ctcatgatgc tttacaacaa tcagattgag
    21661 atagcatcga aggaccagtg gcagatcctc ttcaactgca ttctcgccat cggcgcttgg
    21721 tgtattgaag gcgagagcac agatatcgac gtgttttact atcaaaacgc caagtcgcac
    21781 ctcacctcga aggtcttcga aagtggaagc attatcctcg tgacagccct ccatctgctg
    21841 agtcgttata cccagtggcg tcagaaaact aatacatcct acaactttca ttcgttcagc
    21901 attcggatgg cgattagcct gggacttaat cgtgacctcc caagtagctt cagcgattcg
    21961 tcgatcctgg aacagcgccg gaggatttgg tggagtgtgt acagctggga gatccagctg
    22021 tccctcttgt acggacgcag cattcagctg agtcagaaca cgatttcctt cccgtccagc
    22081 gtggacgacg ttcaacggac aactacgggc ccgaccatct accacggtat cattgagacg
    22141 gcccgcttgc ttcaagtgtt tacgaagatt tatgagctgg ataagacagt gaccgcggag
    22201 aagagtccga tctgcgccaa gaaatgcctg atgatctgca atgagataga ggaggtgagc
    22261 cgccaggcac ccaagttcct gcagatggac ataagcacta cggcgctgac taatctcctc
    22321 aaagaacacc cctggctgag tttcacccgc ttcgagctga agtggaaaca gctgagtctg
    22381 attatatacg ttttgcggga ctttttcaca aattttacgc agaagaagtc gcagcttgag
    22441 caagatcaga acgaccatca gtcctacgag gtaaagaggt gcagcataat gctctccgac
    22501 gcagcccagc gcaccgtgat gtcggtgtcg tcctatatgg acaaccataa tgtgacccct
    22561 tatttcgcct ggaattgctc ctactatttg ttcaacgccg tgttggtccc aatcaagact
    22621 cttctgagca actccaaatc gaacgccgag aataacgaga cagcacagct cttgcagcaa
    22681 attaacaccg tgttgatgct gttgaagaaa ctcgctacat tcaagattca gacttgcgaa
    22741 aagtacatcc aggtgttgga agaggtgtgc gccccattcc ttctttcgca atgcgcaatt
    22801 cccctcccgc acataagtta caataattcc aacggctccg ctatcaagaa tatcgtcggc
    22861 tccgctacaa tcgcgcagta tcccacgctg cctgaggaaa atgttaacaa catatcggtg
    22921 aagtatgtaa gtccaggcag cgtcggcccg agccctgtgc cgctgaaaag cggagcctcc
    22981 ttcagcgatc tggtcaagct gctcagcaac cgcccaccat cccgcaactc ccctgtcact
    23041 ataccccgat cgaccccgtc gcatcgtagc gttaccccat tcctgggaca acagcagcaa
    23101 ctgcagagtc tggtgccact tacgccctcg gcattgtttg gcggagccaa cttcaaccag
    23161 agtggtaaca ttgcggacag ctccttgagc ttcacgttta ccaacagctc caacggtcca
    23221 aacttgatca cgactcagac aaactcccag gcattgtccc agcccattgc cagctcgaat
    23281 gtgcatgata actttatgaa taatgaaatt actgcctcca agattgacga cggcaacaac
    23341 agcaagcccc tttccccagg ctggactgac cagacggcct acaatgcttt cggtatcacc
    23401 actggcatgt tcaacaccac aacgatggac gacgtatata actatctttt tgatgatgag
    23461 gatactccac cgaacccaaa gaaggagtag cctgcaggaa tgaatcgttt ttaaaataac
    23521 aaatcaattg ttttataata ttcgtacgat tctttgatta tgtaataaaa tgtgatcatt
    23581 aggaagatta cgaaaaatat aaaaaatatg agttctgtgt gtataacaaa tgctgtaaac
    23641 gccacaattg tgtttgttgc aaataaaccc atgattattt gattaaaatt gttgttttct
    23701 ttgttcatag acaatagtgt gttttgccta aacgtgtact gcataaactc catgcgagtg
    23761 tatagcgagc tagtggctaa cgcttgcccc accaaagtag attcgtcaaa atcctcaatt
    23821 tcatcaccct cctccaagtt taacatttgg ccgtcggaat taacttctaa agatgccaca
    23881 taatctaata aatgaaatag agattcaaac gtggcgtcat cgtccgtttc gaccatttcc
    23941 gaaaagaact cgggcataaa ctctatgatt tctctggacg tggtgttgtc gaaactctca
    24001 aagtacgcag tcaggaacgt gcgcgacatg tcgtcgggaa actcgcgcgg aaacatgttg
    24061 ttgtaaccga acgggtccca tagcgccaaa accaaatctg ccagcgtcaa tagaatgagc
    24121 acgatgccga caatggagct ggcttggata gcgattcgag ttaactctag aagatcttgg
    24181 ccacgtaata agtgtgcgtt gaatttattc gcaaaaacat tgcatatttt cggcaaagta
    24241 aaattttgtt gcatacctta tcaaaaaata agtgctgcat actttttaga gaaaccaaat
    24301 aattttttat tgcatacccg tttttaataa aatacattgc ataccctctt ttaataaaaa
    24361 atattgcata ctttgacgaa acaaattttc gttgcatacc caataaaaga ttattatatt
    24421 gcatacccgt ttttaataaa atacattgca taccctcttt taataaaaaa tattgcatac
    24481 gttgacgaaa caaattttcg ttgcataccc aataaaagat tattatattg catacctttt
    24541 cttgccatac catttagccg atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa
    24601 ccaacaacgg atccactagt gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
    24661 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
    24721 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
    24781 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
    24841 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
    24901 tcgacactag ttctagccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg
    24961 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    25021 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    25081 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    25141 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    25201 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    25261 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    25321 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    25381 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    25441 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    25501 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    25561 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    25621 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    25681 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    25741 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    25801 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    25861 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    25921 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    25981 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    26041 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    26101 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    26161 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    26221 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    26281 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    26341 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    26401 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    26461 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    26521 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    26581 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    26641 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    26701 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    26761 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    26821 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    26881 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    26941 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    27001 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    27061 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
