LOCUS       sequence-341827-        8725 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Destination transgenesis vector for expressing mCherry fused with an
            optimized photoactivatable Rac1 protein in zebrafish spinal motor 
            neurons using 3 tandem repeats of the mnx1 enhancer..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Harris JM, Wang AY, Boulanger-Weill J, Santoriello C, Foianini S, 
            Lichtman JW, Zon LI, Arlotta P
  TITLE     Long-Range Optogenetic Control of Axon Guidance Overcomes 
            Developmental Boundaries and Defects.
  JOURNAL   Dev Cell. 2020 Jun 8;53(5):577-588.e7. doi: 
            10.1016/j.devcel.2020.05.009.
  PUBMED    32516597
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2020.05"; journalName: "Dev Cell"; date: 
            "2020-06-8- 8"; volume: "53"; issue: "5"; pages: "577-588.e7"
COMMENT     Sequence Label: pDestTol2CG2-mnx1:mCherry-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..8725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1165..1181
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1201..1221
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1665..1689
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1844..1861
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1868..2572
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2010..2028)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2278..2297
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3594..3618)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3620..3754
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3817..3837)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3845..3861)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3877..4011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(4313..5026,5027..5029,5030..5032))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4316..5026,5027..5029,5030..5032))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4358..4379)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4707..4726
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4966..4987
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6488..6504)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6488..6504)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6541..6561)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6541..6559)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6580..6596)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6593..6615)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6604..6620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6628..6634,6635..6652,6653..6658))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6673..6694
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6811..6828)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6982..7570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7062..7081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7741..8532,8533..8601))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8364..8383
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8602..8706)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa aggctggcta agaactcatc agcctccccg
      361 gtccatctac ccacgtacca atgcaccaat tggccacaat gacggctact acatggtgcc
      421 attccttcct ctttatagga atggagacta cctcctgtcc aacaatgctc ttggatacga
      481 gtacgcctac ctgttggacc caggtcattg cacaacacca gaaatgccct ctgatctgca
      541 aaagacgtga atatctgttc agacacccat atccactctg ttccacacag gtcagaggtt
      601 tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
      661 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
      721 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag
      781 taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa
      841 atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta
      901 taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac
      961 ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa
     1021 agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta
     1081 gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa
     1141 acacaggcca gatgggccct cgagcaggaa acagctatga ccatgattac gccaagctat
     1201 caactttgta tagaaaagtt ggctccgaat tcgccctttc ctcgagagtg gttagctgat
     1261 gaattgagga aaactaatcc gctttattgg tagacaggtt aagggcaaca gagtgtcaat
     1321 aattctctcc ctgacctctt tctccattag cttaaagagg cttataagag ggcatgccga
     1381 gagtggttag ctgatgaatt gaggaaaact aatccgcttt attggtagac aggttaaggg
     1441 caacagagtg tcaataattc tctccctgac ctctttctcc attagcttaa agaggcttat
     1501 aagaggccta ggcgagagtg gttagctgat gaattgagga aaactaatcc gctttattgg
     1561 tagacaggtt aagggcaaca gagtgtcaat aattctctcc ctgacctctt tctccattag
     1621 cttaaagagg cttataagag gaagcttgta agggcgaatt cgacccaagt ttgtacaaaa
     1681 aagcaggctc cgcggccaat ctgctcagag aggacagagt gggcaggagc cagcattggg
     1741 tatataaagc tgagcagggt cagttgcttc ttacgtttgc ttctgagtct gttgtgttga
     1801 cttgcaacct cagaaacaga catccgcccc cttcaccatg gcacaccatc accaccatca
     1861 cggatctgtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     1921 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     1981 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     2041 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     2101 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     2161 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     2221 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     2281 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     2341 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     2401 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     2461 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     2521 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agggatcctt
     2581 ggctactaca cttgaacgta ttgagaagaa ctttgtcatt actgacccaa gattgccaga
     2641 taatcccatt atattcgcgt ccgatagttt cttgcagttg acagaatata gccgtgaaga
     2701 aattttggga agaaactgca ggtttctaca aggtcctgaa actgatcgcg cgacagtgag
     2761 aaaaattaga gatgccatag ataaccaaac agaggtcact gttcagctga ttaattatac
     2821 aaagagtggt aaaaagttct ggaacctctt tcacttgcag cctatgcgag atcagaaggg
     2881 agatgtccag tactttattg gggttcagaa ggatggaact gagcatgtcc gagatgctgc
     2941 cgagagagag ggagtcatgg agattaagaa aactgcagaa aatattgatg aggcggcaaa
     3001 agaacttatc aagtgtgtgg tggtgggaga cggagctgta ggtaaaactt gcctactgat
     3061 cagttacaca accaatgcat ttcctggaga atatatccct actgtctttg acaattattc
     3121 tgccaatgtt atggtagatg gaaaaccggt gaatctgggc ttatgggata cagctggact
     3181 agaagattat gacagattac gccccctatc ctatccgcaa acagatgtgt tcttaatttg
     3241 cttttccctt gtgagtcctg catcattcca ccacgtccgt gcaaagtggt atcctgaggt
     3301 gcggcaccac tgtcccaaca ctcccatcat cctagtggga actaaacttg atcttaggga
     3361 tgataaagac acgatcgaga aactgaagga gaagaagctg actcccatca cctatccgca
     3421 gggtctagcc atggctaagg agattggtgc tgtaaaatac ctggagtgct cggcgctcac
     3481 acagcgaggc ctcaagacag tgtttgacga agcgatccga gcagtcctct gcccgcctcc
     3541 cgtgaagaag aggaagagaa aatgcctgct gttgtaaaag ggtgggcgcg ccgacccagc
     3601 tttcttgtac aaagtggggg atccagacat gataagatac attgatgagt ttggacaaac
     3661 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     3721 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     3781 gtttcaggtt cagggggagg tgtgggaggt tttttccaac tttattatac atagttgata
     3841 attcactggc cgtcgtttta cggtaccatc gatgatgatc cagacatgat aagatacatt
     3901 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     3961 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     4021 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     4081 taaaacctct acaaatgtgg tatggctgat tatgatcctc tagatcagat ctcttgttta
     4141 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4201 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4261 ggatctacgt aatacgactc actatagttc tagaggctcg agaggggccg ctttacttgt
     4321 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
     4381 gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt
     4441 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
     4501 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct
     4561 gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca
     4621 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
     4681 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
     4741 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
     4801 ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc
     4861 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg
     4921 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
     4981 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtcactg
     5041 tctgctttgc tgttggtctg ggctcctggg tcactggctt actaatggag tctttatgta
     5101 tgaggactct tatcaattgt tcttctataa aggtctgcag tgtttctgtt cgtcccctac
     5161 atggacaccc agagcctcct aaatacagga gccctgataa ctgcacaagt gctcagattc
     5221 cagcagggtg gaaaatgaga taaagtgtgc agatggggag ggggacgtga atgagagatt
     5281 tgagggatga aaaggatgga tgaacgcatt gaaaatagcc cctttcacaa aataatacca
     5341 gtaaattgtc atataattaa tgaccagacc tttacctgta aatgatgtga tgttcatata
     5401 cagaagaatg ccaacaattt acagatgatt ttacaaattc ttaattttcc ttttctaaat
     5461 ggatttgttc acacattatc tcttcaaagc aattgaattt tctggaaaag attggctgtg
     5521 tgtgtaaaag gtgctactga ggaatttaat gtgacatgga atgaagcaaa cagcagccta
     5581 agacaggatg gcaggaaaaa tgtcacatgt ttaaaataag agtgaaacca acgacctgga
     5641 cttatagaga ctgtgtgtcc ttaggcaaag ctcttgtaca aacagtgctt gtgactgtga
     5701 aattcggggt ttgcctggat tgtgttaaat gtgtgcttgg atgtatcata aaaaagaatc
     5761 ggttctctgt atttgacatt ttctgtaatt tatctaaagt tacaactggc tatgcctgat
     5821 ttttatcact attgaatcaa tcattttaaa gaatgttaag tctgctgatt tggagagctc
     5881 atttacattt attttgaatg tctcttattt aacacaactg atttaagctt tagatctgcg
     5941 aagatacggc cacgggtgct cttgatcctg tggctgattt tggactgtgc tgctcgcagc
     6001 tgctgatgaa tcacatactt cctccatttt cttccactga ttgactgtta taatttccct
     6061 aatttccagg tcaaggtgct gtgcattgtg gtaatagatg tgacatgacg tcacttccaa
     6121 aggaccaatg aacatgtctg accaatttca tataatgtga aaacgatttt cataggcaga
     6181 ataaataaca tttaaattaa actgggcatc agcgcaattc aattggtttg gtaatagcaa
     6241 gggaaaatag aatgaagtga tctccaaaaa ataagtactt tttgactgta aataaaattg
     6301 taaggagtaa aaagtacttt tttttctaaa aaaatgtaat taagtaaaag taaaagtatt
     6361 gatttttaat tgtactcaag taaagtaaaa atccccaaaa ataatactta agtacagtaa
     6421 tcaagtaaaa ttactcaagt actttacacc tctggttctt gaccccctac cttcagcaag
     6481 cccagcagat ccactagttc tagagcggcc gccaccgcgg tggagctcca gcttttgttc
     6541 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6601 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6661 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6721 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6781 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6841 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6901 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6961 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7021 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7081 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7141 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7201 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7261 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7321 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7381 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7441 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7501 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7561 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7621 ctcacgttaa ggcattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7681 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7741 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7801 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7861 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7921 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7981 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8041 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8101 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8161 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8221 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8281 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8341 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8401 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8461 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8521 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8581 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8641 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8701 tccgcgcaca tttccccgaa aagtg
//
