LOCUS       sequence-342254-        2094 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  SynZip1 linked surfactant protein that localizes to RGG condensate 
            interface.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Guan M, Garabedian MV, Leutenegger M, Schuster BS, Good MC, Hammer 
            DA
  TITLE     Incorporation and Assembly of a Light-Emitting Enzymatic Reaction 
            into Model Protein Condensates.
  JOURNAL   Biochemistry. 2021 Oct 14. doi: 10.1021/acs.biochem.1c00373.
  PUBMED    34648259
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2021 Oct 14. doi: 10.1021/acs.biochem.1c00373."
COMMENT     Sequence Label: SZ1-GST-mCherry-RGG
FEATURES             Location/Qualifiers
     source          1..2094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             148..798
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPP"
     primer_bind     759..781
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             1528..1548
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2074..2091
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgaacttgg tagcccagct tgagaacgaa gttgcgagtc tggaaaacga aaacgagacg
       61 ttgaagaaaa agaacttgca caagaaagat ttgattgcct acttggaaaa ggaaattgcc
      121 aatctgcgta aaaagattga ggaatcgatg tcccctatac taggttattg gaaaattaag
      181 ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat
      241 ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag
      301 tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc
      361 atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag
      421 atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat
      481 agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa
      541 atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct
      601 gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat
      661 gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag
      721 tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt
      781 ggtggcgacc atcctccagg atccggtatg gtcagcaagg gcgaggagga caacatggcg
      841 atcatcaagg agtttatgcg gttcaaggtg cacatggaag gttctgtcaa tggacacgaa
      901 tttgaaatcg agggtgaggg ggaaggacgc ccatacgagg ggacccaaac tgcgaagctt
      961 aaagttacaa aggggggtcc gttgccgttt gcttgggata tcttatcacc gcagttcatg
     1021 tatggcagca aagcttacgt caaacaccca gcagatattc ctgattatct taaattgtcg
     1081 ttccccgaag gcttcaagtg ggaacgggta atgaacttcg aagatggagg ggtagttacg
     1141 gtcacccagg attcttctct gcaagatggc gagttcattt acaaagtgaa acttagaggc
     1201 acaaatttcc cctctgatgg acccgttatg cagaagaaaa cgatggggtg ggaggcatct
     1261 agtgagcgta tgtatccaga agatggggct ttaaaagggg agatcaaaca gcgtctgaag
     1321 ttgaaagacg gcggtcatta tgatgccgag gtcaaaacta cgtacaaggc aaaaaagcca
     1381 gttcagttgc ctggtgctta caacgtgaat atcaaacttg atatcacaag ccacaatgag
     1441 gattatacca ttgttgaaca atatgaaaga gcagagggcc ggcacagtac aggcggtatg
     1501 gatgagttgt ataaaggcgg cggatctgag aatttatatt tccagggcga attcgggaag
     1561 cttatggaat ccaatcaatc caataacggt ggctcgggta atgccgctct gaatcgtggt
     1621 ggccgctacg tgccgccgca tctgcgtggt ggcgatggtg gtgcagcagc agctgcatct
     1681 gctggcggtg atgaccgtcg cggcggtgcg ggcggtggcg gttatcgtcg cggcggtggc
     1741 aatagtggtg gcggtggcgg tggcggttat gatcgtggtt acaatgacaa ccgtgatgac
     1801 cgcgataacc gtggcggttc cggcggttac ggtcgtgatc gtaattatga agaccgtggt
     1861 tacaacggcg gtggcggtgg cggtggcaat cgtggctata acaataaccg tggtggcggt
     1921 ggcggtggct acaatcgcca ggatcgtggt gacggtggca gctctaactt ttcgcgcggt
     1981 ggctataata accgtgatga aggcagcgac aaccgcggta gtggccgttc ctacaataac
     2041 gatcgtcgcg acaatggtgg cgatggcctc gagcaccacc accaccacca ctga
//
