LOCUS       sequence-342308-        3597 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  EphrinA1 RTK fusion to Cry2olig - includes SAM domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Wurz AI, Zheng KS, Hughes RM
  TITLE     Optogenetic Regulation of EphA1 RTK Activation and Signaling.
  JOURNAL   bioRxiv [Preprint]. 2024 Feb 7:2024.02.06.579139. doi: 
            10.1101/2024.02.06.579139.
  PUBMED    38370612
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Feb 7:2024.02.06.579139. doi: 
            10.1101/2024.02.06.579139."
COMMENT     Sequence Label: EphA1-SAM-Cry2olig
FEATURES             Location/Qualifiers
     source          1..3597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1369..2862
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     regulatory      2881..2890
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2887..3597
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3032..3050)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3300..3319
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggtgtcca ggggcctgac tggaggagag attgtagccg tcatctttgg gctgctgctt
       61 ggtgcagcct tgctgcttgg gattctcgtt ttccggtcca ggagagccca gcggcagagg
      121 cagcagaggc agcgtgaccg cgccaccgat gtggatcgag aggacaagct gtggctgaag
      181 ccttatgtgg acctccaggc atacgaggac cctgcacagg gagccttgga ctttacccgg
      241 gagcttgatc cagcgtggct gatggtggac actgtcatag gagaaggaga gtttggggaa
      301 gtgtatcgag ggaccctgag gctccccagc caggactgca agactgtggc cattaagacc
      361 ttaaaagaca catccccagg tggccagtgg tggaacttcc ttcgagaggc aactatcatg
      421 ggccagttta gccacccgca tattctgcat ctggaaggcg tcgtcacaaa gcgaaagccg
      481 atcatgatca tcacagaatt tatggagaat ggagccctgg atgccttcct gagggagcgg
      541 gaggaccagc tggtccctgg gcagctagtg gccatgctgc agggcatagc atctggcatg
      601 aactacctca gtaatcacaa ttatgtccac cgggacctgg ctgccagaaa catcttggtg
      661 aatcaaaacc tgtgctgcaa ggtgtctgac tttggcctga ctcgcctcct ggatgacttt
      721 gatggcacat acgaaaccca gggaggaaag atccctatcc gttggacagc ccctgaagcc
      781 attgcccatc ggatcttcac cacagccagc gatgtgtgga gctttgggat tgtgatgtgg
      841 gaggtgctga gctttgggga caagccttat ggggagatga gcaatcagga ggttatgaag
      901 agcattgagg atgggtaccg gttgccccct cctgtggact gccctgcccc tctgtatgag
      961 ctcatgaaga actgctgggc atatgaccgt gcccgccggc cacacttcca gaagcttcag
     1021 gcacatctgg agcaactgct tgccaacccc cactccctgc ggaccattgc caactttgac
     1081 cccagggtga ctcttcgcct gcccagcctg agtggctcag atgggatccc gtatcgaacc
     1141 gtctctgagt ggctcgagtc catacgcatg aaacgctaca tcctgcactt ccactcggct
     1201 gggctggaca ccatggagtg tgtgctggag ctgaccgctg aggacctgac gcagatggga
     1261 atcacactgc ccgggcacca gaagcgcatt ctttgcagta ttcagggatt caaggacttg
     1321 ggcggcagcg actcgctagc gctaccggac tcagatctcg aggccaccat gaagatggac
     1381 aaaaagacta tagtttggtt tagaagagac ctaaggattg aggataatcc tgcattagca
     1441 gcagctgctc acgaaggatc tgtttttcct gtcttcattt ggtgtcctga agaagaagga
     1501 cagttttatc ctggaagagc ttcaagatgg tggatgaaac aatcacttgc tcacttatct
     1561 caatccttga aggctcttgg atctgacctc actttaatca aaacccacaa cacgatttca
     1621 gcgatcttgg attgtatccg cgttaccggt gctacaaaag tcgtctttaa ccacctctat
     1681 gatcctgttt cgttagttcg ggaccatacc gtaaaggaga agctggtgga acgtgggatc
     1741 tctgtgcaaa gctacaatgg agatctattg tatgaaccgt gggagatata ctgcgaaaag
     1801 ggcaaacctt ttacgagttt caattcttac tggaagaaat gcttagatat gtcgattgaa
     1861 tccgttatgc ttcctcctcc ttggcggttg atgccaataa ctgcagcggc tgaagcgatt
     1921 tgggcgtgtt cgattgaaga actagggctg gagaatgagg ccgagaaacc gagcaatgcg
     1981 ttgttaacta gagcttggtc tccaggatgg agcaatgctg ataagttact aaatgagttc
     2041 atcgagaagc agttgataga ttatgcaaag aacagcaaga aagttgttgg gaattctact
     2101 tcactacttt ctccgtatct ccatttcggg gaaataagcg tcagacacgt tttccagtgt
     2161 gcccggatga aacaaattat atgggcaaga gataagaaca gtgaaggaga agaaagtgca
     2221 gatctttttc ttaggggaat cggtttaaga gagtattctc ggtatatatg tttcaacttc
     2281 ccgtttactc acgagcaatc gttgttgagt catcttcggt ttttcccttg ggatgctgat
     2341 gttgataagt tcaaggcctg gagacaaggc aggaccggtt atccgttggt ggatgccgga
     2401 atgagagagc tttgggctac cggatggatg cataacagaa taagagtgat tgtttcaagc
     2461 tttgctgtga agtttcttct ccttccatgg aaatggggaa tgaagtattt ctgggataca
     2521 cttttggatg ctgatttgga atgtgacatc cttggctggc agtatatctc tgggagtatc
     2581 cccgatggcc acgagcttga tcgcttggac aatcccgcgt tacaaggcgc caaatatgac
     2641 ccagaaggtg agtacataag gcaatggctt cccgagcttg cgagattgcc aactgaatgg
     2701 atccatcatc catgggacgc tcctttaacc gtactcaaag cttctggtgt ggaactcgga
     2761 acaaactatg cgaaacccat tgtagacatc gacacagctc gtgagctact agctaaagct
     2821 atttcaagaa cccgtggagc acagatcatg atcggagcag cagcccggga tccaccggtc
     2881 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     2941 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     3001 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     3061 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     3121 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     3181 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     3241 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     3301 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     3361 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     3421 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     3481 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     3541 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaa
//
