LOCUS       sequence-342430-       10982 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Cas9 gap repair vector - ZeoR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10982)
  TITLE     Ellis Lab Yeast CRISPR Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pWS176
FEATURES             Location/Qualifiers
     source          1..10982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             778..4881
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             778..4881
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4894..4914
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4918..4938
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             5848..5859
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5866..6579
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      6591..6662
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6678..6705
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_RNA        6719..6794
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      8028..8225
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      8230..9212
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(9270..10085)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9270..10085)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9993..10012
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10077..10096
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10310..10898
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10799..10818
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgtggata accgtgcggc cgcccctgaa ttcgcatcta gactgaactg gccgataatt
       61 gcagacgaac ggtgagtaag gaaagagtga ggaactatcg catacctgca tttaaagatg
      121 ccgatttggg cgcgaatcct ttattttggc ttcaccctca tactattatc agggccagaa
      181 aaaggaagtg tttccctcct tcttgaattg atgttaccct cataaagcac gtggcctctt
      241 atcgagaaag aaattaccgt cgctcgtgat ttgtttgcaa aaagaacaaa actgaaaaaa
      301 cccagacacg ctcgacttcc tgtcatccta ttgattgcag cttccaattt cgtcacacaa
      361 caaggtccta gcgacggctc acaggttttg taacaagcaa tcgaaggttc tggaatggcg
      421 ggaaagggtt tagtaccaca tgctatgatg cccactgtga tctccagagc aaagttcgtt
      481 cgatcgtact gttactctct ctctttcaaa cagaattgtc cgaatcgtgt gacaacaaca
      541 gcctgttctc acacactctt ttcttctaac caagggggtg gtttagttta gtagaacctc
      601 gtgaaactta catttacata tatataaact tgcataaatt ggtcaatgca agaaatacat
      661 atttggtctt ttctaattcg tagtttttca agttcttaga tgctttcttt ttctcttttt
      721 tacagatcat caaggaagta attatctact ttttacaaca aatataaaac aagatctatg
      781 gacaagaagt attctatcgg actggatatc gggactaata gcgtcgggtg ggccgtgatc
      841 actgacgagt acaaggtgcc ctctaagaag ttcaaggtgc tcgggaacac cgaccggcat
      901 tccatcaaga aaaatctgat cggagctctc ctctttgatt caggggaaac cgctgaagca
      961 acccgcctca agcggactgc tagacggcgg tacaccagga ggaagaaccg gatttgttac
     1021 cttcaagaga tattctccaa cgaaatggca aaggtcgacg acagcttctt ccataggctg
     1081 gaagaatcat tcctcgtgga agaggataag aagcatgaac ggcatcccat cttcggtaat
     1141 atcgtcgacg aggtggccta tcacgagaaa tacccaacca tctaccatct tcgcaaaaag
     1201 ctggtggact caaccgacaa ggcagacctc cggcttatct acctggccct ggcccacatg
     1261 atcaagttca gaggccactt cctgatcgag ggcgacctca atcctgacaa tagcgatgtg
     1321 gataaactgt tcatccagct ggtgcagact tacaaccagc tctttgaaga gaaccccatc
     1381 aatgcaagcg gagtcgatgc caaggccatt ctgtcagccc ggctgtcaaa gagccgcaga
     1441 cttgagaatc ttatcgctca gctgccgggt gaaaagaaaa atggactgtt cgggaacctg
     1501 attgctcttt cacttgggct gactcccaat ttcaagtcta atttcgacct ggcagaggat
     1561 gccaagctgc aactgtccaa ggacacctat gatgacgatc tcgacaacct cctggcccag
     1621 atcggtgacc aatacgccga ccttttcctt gctgctaaga atctttctga cgccatcctg
     1681 ctgtctgaca ttctccgcgt gaacactgaa atcaccaagg cccctctttc agcttcaatg
     1741 attaagcggt atgatgagca ccaccaggac ctgaccctgc ttaaggcact cgtccggcag
     1801 cagcttccgg agaagtacaa ggaaatcttc tttgaccagt caaagaatgg atacgccggc
     1861 tacatcgacg gaggtgcctc ccaagaggaa ttttataagt ttatcaaacc tatccttgag
     1921 aagatggacg gcaccgaaga gctcctcgtg aaactgaatc gggaggatct gctgcggaag
     1981 cagcgcactt tcgacaatgg gagcattccc caccagatcc atcttgggga gcttcacgcc
     2041 atccttcggc gccaagagga cttctacccc tttcttaagg acaacaggga gaagattgag
     2101 aaaattctca ctttccgcat cccctactac gtgggacccc tcgccagagg aaatagccgg
     2161 tttgcttgga tgaccagaaa gtcagaagaa actatcactc cctggaactt cgaagaggtg
     2221 gtggacaagg gagccagcgc tcagtcattc atcgaacgga tgactaactt cgataagaac
     2281 ctccccaatg agaaggtcct gccgaaacat tccctgctct acgagtactt taccgtgtac
     2341 aacgagctga ccaaggtgaa atatgtcacc gaagggatga ggaagcccgc attcctgtca
     2401 ggcgaacaaa agaaggcaat tgtggacctt ctgttcaaga ccaatagaaa ggtgaccgtg
     2461 aagcagctga aggaggacta tttcaagaaa attgaatgct tcgactctgt ggagattagc
     2521 ggggtcgaag atcggttcaa cgcaagcctg ggtacttacc atgatctgct taagatcatc
     2581 aaggacaagg attttctgga caatgaggag aacgaggaca tccttgagga cattgtcctg
     2641 actctcactc tgttcgagga ccgggaaatg atcgaggaga ggcttaagac ctacgcccat
     2701 ctgttcgacg ataaagtgat gaagcaactt aaacggagaa gatataccgg atggggacgc
     2761 cttagccgca aactcatcaa cggaatccgg gacaaacaga gcggaaagac cattcttgat
     2821 ttccttaaga gcgacggatt cgctaatcgc aacttcatgc aacttatcca tgatgattcc
     2881 ctgaccttta aggaggacat ccagaaggcc caagtgtctg gacaaggtga ctcactgcac
     2941 gagcatatcg caaatctggc tggttcaccc gctattaaga agggtattct ccagaccgtg
     3001 aaagtcgtgg acgagctggt caaggtgatg ggtcgccata aaccagagaa cattgtcatc
     3061 gagatggcca gggaaaacca gactacccag aagggacaga agaacagcag ggagcggatg
     3121 aaaagaattg aggaagggat taaggagctc gggtcacaga tccttaaaga gcacccggtg
     3181 gaaaacaccc agcttcagaa tgagaagctc tatctgtact accttcaaaa tggacgcgat
     3241 atgtatgtgg accaagagct tgatatcaac aggctctcag actacgacgt ggaccatatc
     3301 gtccctcaga gcttcctcaa agacgactca attgacaata aggtgctgac tcgctcagac
     3361 aagaaccggg gaaagtcaga taacgtgccc tcagaggaag tcgtgaaaaa gatgaagaac
     3421 tattggcgcc agcttctgaa cgcaaagctg atcactcagc ggaagttcga caatctcact
     3481 aaggctgaga ggggcggact gagcgaactg gacaaagcag gattcattaa acggcaactt
     3541 gtggagactc ggcagattac taaacatgtc gcccaaatcc ttgactcacg catgaatacc
     3601 aagtacgacg aaaacgacaa acttatccgc gaggtgaagg tgattaccct gaagtccaag
     3661 ctggtcagcg atttcagaaa ggactttcaa ttctacaaag tgcgggagat caataactat
     3721 catcatgctc atgacgcata tctgaatgcc gtggtgggaa ccgccctgat caagaagtac
     3781 ccaaagctgg aaagcgagtt cgtgtacgga gactacaagg tctacgacgt gcgcaagatg
     3841 attgccaaat ctgagcagga gatcggaaag gccaccgcaa agtacttctt ctacagcaac
     3901 atcatgaatt tcttcaagac cgaaatcacc cttgcaaacg gtgagatccg gaagaggccg
     3961 ctcatcgaga ctaatgggga gactggcgaa atcgtgtggg acaagggcag agatttcgct
     4021 accgtgcgca aagtgctttc tatgcctcaa gtgaacatcg tgaagaaaac cgaggtgcaa
     4081 accggaggct tttctaagga atcaatcctc cccaagcgca actccgacaa gctcattgca
     4141 aggaagaagg attgggaccc taagaagtac ggcggattcg attcaccaac tgtggcttat
     4201 tctgtcctgg tcgtggctaa ggtggaaaaa ggaaagtcta agaagctcaa gagcgtgaag
     4261 gaactgctgg gtatcaccat tatggagcgc agctccttcg agaagaaccc aattgacttt
     4321 ctcgaagcca aaggttacaa ggaagtcaag aaggacctta tcatcaagct cccaaagtat
     4381 agcctgttcg aactggagaa tgggcggaag cggatgctcg cctccgctgg cgaacttcag
     4441 aagggtaatg agctggctct cccctccaag tacgtgaatt tcctctacct tgcaagccat
     4501 tacgagaagc tgaaggggag ccccgaggac aacgagcaaa agcaactgtt tgtggagcag
     4561 cataagcatt atctggacga gatcattgag cagatttccg agttttctaa acgcgtcatt
     4621 ctcgctgatg ccaacctcga taaagtcctt agcgcataca ataagcacag agacaaacca
     4681 attcgggagc aggctgagaa tatcatccac ctgttcaccc tcaccaatct tggtgcccct
     4741 gccgcattca agtacttcga caccaccatc gaccggaaac gctatacctc caccaaagaa
     4801 gtgctggacg ccaccctcat ccaccagagc atcaccggac tttacgaaac tcggattgac
     4861 ctctcacagc tcggagggga tgagggagct gatccaaaaa agaagagaaa ggtagatcca
     4921 aaaaagaaga gaaaggtata aggatcctaa ctcgagattg aattgaattg aaatcgatag
     4981 atcaattttt ttcttttctc tttccccatc ctttacgcta aaataatagt ttattttatt
     5041 ttttgaatat tttttattta tatacgtata tatagactat tatttatctt ttaatgatta
     5101 ttaagatttt tattaaaaaa aaattcgctc ctcttttaat gcctttatgc agtttttttt
     5161 tcccattcga tatttctatg tgctgccaag atatcaacaa cagaggacat atgccctacc
     5221 tccatgatct actgacgtcc ctgaggctgc aattcatgta atggggcagt atccgcggca
     5281 agtcctagtg caatggcggt tttttaccct cgttctgaag aagaggcgac gcgggtgcgg
     5341 tcatcactaa tgtggaaatt ggtaagactc tcgggcctcc gcctttaggc ggtgcttact
     5401 ctttcataaa ggggctgtta gttatgcaac gatgtgcttc agtattacat tttttgcctt
     5461 caacgccttg attgttctat ttttgctaat aataaatcta tttcatcgga ctaaaagtcc
     5521 attagttgta agcggattta gctcagttgg gagagcgcca gactgaagaa aaacttcggt
     5581 caagtcatct ggaggtcctg tgttcgatcc acagaattcg cagatggccg gcatggtccc
     5641 agcctcctcg ctggcgccgg ctgggcaaca ccttcgggtg gcgaatggga cttgagacgg
     5701 aaagtgaaac gtgatttcat gcgtcatttt gaacattttg taaatcttat ttaataatgt
     5761 gtgcggcaat tcacatttaa tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac
     5821 ggcaggttcg gatcttagct actagagaaa gaggagaaat actagatgcg taaaggcgaa
     5881 gagctgttca ctggtgtcgt ccctattctg gtggaactgg atggtgatgt caacggtcat
     5941 aagttttccg tgcgtggcga gggtgaaggt gacgcaacta atggtaaact gacgctgaag
     6001 ttcatctgta ctactggtaa actgccggtt ccttggccga ctctggtaac gacgctgact
     6061 tatggtgttc agtgctttgc tcgttatccg gaccatatga agcagcatga cttcttcaag
     6121 tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt cctttaagga tgacggcacg
     6181 tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg cattgagctg
     6241 aaaggcattg actttaaaga ggacggcaat atcctgggcc ataagctgga atacaatttt
     6301 aacagccaca atgtttacat caccgccgat aaacaaaaaa atggcattaa agcgaatttt
     6361 aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac
     6421 actccaatcg gtgatggtcc tgttctgctg ccagacaatc actatctgag cacgcaaagc
     6481 gttctgtcta aagatccgaa cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc
     6541 gcagcgggca tcacgcatgg tatggatgaa ctgtacaaat gaccaggcat caaataaaac
     6601 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     6661 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat acgtctcagt
     6721 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
     6781 caccgagtcg gtgctttttt tattttttgt cactattgtt atgtaaaatg ccacctctga
     6841 cagtatggaa cgcaaacttc tgtctagtgg atagctggac attccaggca gtgcctctgc
     6901 cgccggaccc ctctcgtgat tgggtagctg gacatgccct tgtaagatat aacaagagcc
     6961 tgcctgtcta atgatctcac ggcgaaagtc ggggagacag cagcggctgc agacattata
     7021 ccgcaacaac actaaggtga gataactccg tacattgact acgcgttcct ctagacctta
     7081 cttgaccgga tacagtgtct ttgacacgtt tatgggttac agcaatcaca tccaagactg
     7141 gctatgcacg aagcaactct tgagtgttaa aatgttgacc cctgtatttg ggatgcgggt
     7201 agtagatgag tgcaggatat cagcagttac agagatgtta cgaaccacta gtgcactgca
     7261 gtacagttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga
     7321 ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt
     7381 acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca
     7441 cggcgcgaag caaaaattac ggctcctcgc tccagacctg cgagcaggga aacgctcccc
     7501 tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg
     7561 atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt
     7621 ctcacatcac atccgaacat aaacaaaaat ggctaaatta acatctgccg ttcctgtttt
     7681 aacagctagg gatgttgcag gtgctgtaga gttttggaca gataggttag gattctcaag
     7741 agactttgtt gaggacgatt ttgctggtgt tgtcagggat gacgttactt tatttatctc
     7801 agcagtccaa gatcaagttg tccctgataa tacattggct tgggtctggg tcaggggttt
     7861 agatgaatta tatgctgaat ggtcagaagt tgtatctaca aacttcagag atgcttctgg
     7921 tccagctatg accgagattg gtgaacagcc atggggtaga gaatttgctt tgagagatcc
     7981 agctggaaat tgtgttcatt ttgttgctga agaacaagat taaagtaact gacaataaaa
     8041 agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat
     8101 tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg
     8161 cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta
     8221 tactggagta acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg
     8281 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa
     8341 gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga
     8401 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
     8461 cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat
     8521 cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc
     8581 tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg
     8641 ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac
     8701 tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc
     8761 tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc
     8821 attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa
     8881 atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt
     8941 ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca
     9001 agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata
     9061 gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc
     9121 gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc atcagagcgc ttttggtttt
     9181 caaaagcgct ctgaagttcc tatactttct agctagagaa taggaacttc ccgagcggcc
     9241 gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac
     9301 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     9361 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     9421 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     9481 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     9541 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     9601 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac gcgatcgctg
     9661 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     9721 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     9781 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     9841 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     9901 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     9961 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
    10021 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
    10081 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
    10141 atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga
    10201 ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct taacgtgagt
    10261 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    10321 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    10381 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10441 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10501 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10561 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10621 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10681 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10741 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10801 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10861 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10921 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10981 at
//
