LOCUS       sequence-342441-        7075 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses recoded mouse Peg10 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Segel M, Lash B, Song J, Ladha A, Liu CC, Jin X, Mekhedov SL, Macrae
            RK, Koonin EV, Zhang F
  TITLE     Mammalian retrovirus-like protein PEG10 packages its own mRNA and 
            can be pseudotyped for mRNA delivery.
  JOURNAL   Science. 2021 Aug 20;373(6557):882-889. doi: 
            10.1126/science.abg6155.
  PUBMED    34413232
REFERENCE   2  (bases 1 to 7075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-08-20- 20"; volume: "373"; 
            issue: "6557"; pages: "882-889"
COMMENT     Sequence Label: pCMV-MmPeg10rc4
FEATURES             Location/Qualifiers
     source          1..7075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          718..1193
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             4146..4172
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4257..4651
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(4990..5007)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5161..5749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5241..5260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5920..6711,6712..6780))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6543..6562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6781..6885)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6955..6974)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccc ctcgaagctt acatgtggta ccgagctcgg atcctgagaa cttcagggtg
      721 agtctatggg acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat
      781 aggaagggga gaagtaacag ggtacacata ttgaccaaat cagggtaatt ttgcatttgt
      841 aattttaaaa aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact
      901 ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca
      961 ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa
     1021 atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac
     1081 aatccagcta ccattctgct tttattttat ggttgggata aggctggatt attctgagtc
     1141 caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg
     1201 gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agcacgtgag atctgaattc
     1261 cccaagctgg ctagcgttta aaaccgccac atggctgctg caggtggttc ctccaactgc
     1321 ccgccccctc cccctccccc tcctcccaac aacaacaaca acaacaacac cccaaagagc
     1381 ccaggcgtgc ctgacgccga agatgatgat gaacgcagac acgatgagct ccctgaagac
     1441 atcaacaact ttgacgaaga catgaacagg cagtttgaga atatgaacct gctggatcag
     1501 gtggagttgc ttgcacagag ctacagtctg ctggatcatt tagatgactt tgatgatgat
     1561 gatgaagacg atgactttga tccagaacct gaccaggatg agctccctga gtacagtgac
     1621 gatgatgacc tggagcttca gggtgctgca gcagccccta tcccaaactt tttctccgat
     1681 gatgactgcc ttgaagacct tcctgagaag ttcgatggca accctgacat gctgggtcct
     1741 ttcatgtatc agtgccagct cttcatggaa aagagcacca gagatttctc agttgaccgc
     1801 atccgtgtgt gcttcgtgac aagcatgctg atcggccgtg ccgcccgctg ggctactgcc
     1861 aagctgcaaa gatgtactta cctgatgcac aactacactg cctttatgat ggagctgaag
     1921 catgtctttg aagaccctca gagacgtgaa gctgccaaac gcaagatcag acgtctgcgc
     1981 cagggccctg ggcctgttgt ggactactcc aatgcattcc agatgattgc ccaggacctg
     2041 gattggactg agcctgccct gatggatcag ttccaggaag gtctcaaccc agacattcgc
     2101 gcagagctgt ctcgccagga ggcccccaag accctggctg ctctgattac tgcctgtatt
     2161 cacatcgaga gaaggctggc tcgtgacgct gctgcaaagc ccgatccttc acccagagcc
     2221 ttggtgatgc ctccaaacag ccagaccgat cccaccgagc ctgtgggagg tgcccgcatg
     2281 cgcctgtcca aggaagaaaa ggagagacgc cgcaaaatga atttgtgtct ctactgtggc
     2341 aatggaggcc atttcgccga cacgtgtcca gcgaaagcct ccaagaattc gccgccggga
     2401 aactccccgg ccccgctgta gggggacctt cagcgacagg gccagaacga ataaggtccc
     2461 caccctccga ggcttcgact cagcacctgc aagtgatgct ccagattcat atgccgggca
     2521 gacccaccct gtttgtccga gctatgattg attctggtgc atctggcaac ttcattgatc
     2581 aagactttgt catacaaaat gcaattcctc tcagaatcaa agactggcca gtgatggtgg
     2641 aagctattga tgggcatcca attgcctcgg gcccaatcat tttggaaacc caccacctga
     2701 tagttgatct gggagaccac cgtgagatac tgtcatttga tgtgactcag tctccattct
     2761 ttcctattgt cctaggaatt cgttggctct ctactcacga cccacacatc acctggagta
     2821 ctcgctccat agtcttcaac tctgattact gtcggttgag atgccgaatg ttcgctcaga
     2881 tcccaagcaa cctgctcttt accgttcccc aaccaaatct ccacccctat ctcctgcatc
     2941 atgtacaccc ccatgtacac cctcacatgc atcaacacct tcatcaacac ctgcatcagt
     3001 ttcttcatcc tgatccacat cagtatccac acccagaccc ccattaccat catcaccagc
     3061 aggctgatat gcagcatcag ctgcaacagt atttgtatca gtacttgtac taccatctgt
     3121 accctgtcat gcatcatcat cttcctcctg accaacatga acatctgcac gaatatcttc
     3181 accaatacct ccatcagtac cttcaccaat tcctccacca ccatcttcac cctgacttgc
     3241 accaatattt gtaccagtat cttcacaacc acatgaatcc agatccacat caccatcccc
     3301 atccagatcc ccctcaggat ccacatcacc ctccacatca ggatccccat caggatcctc
     3361 cacatcagga tccacatcag gatgcacatc aggatcccca tatggatcca cacctgcatc
     3421 agcaccagca tccgcagccg cagccgcatc cacaacagca tcctaaccat cctcagcagc
     3481 caccattctt ctaccacatg gctggattca gaatttacca ccctgtaagg tattactata
     3541 ttcagaatgt gtatacacct gttgatgagc atgtctatcc gggtcaccgg gtggttgacc
     3601 ctaacattga gatgattcct ggagcgcaca gcctgcccag tggacatttg tactcaatgt
     3661 ctgagtctga aatgaatgct ctgcgaaatt tcgtggacag gaatgttaaa gatgggctca
     3721 tgactcccac tgtggcgccc aatggagccc aagtcctgca agtgaaaaga gggtggaaac
     3781 tccaagtcac ttacaattgc cgagctccac agagtggcac catccaaaat cagtacctac
     3841 gcatgtctct tccaaatatg ggagaccctg cacacctggc aagctatggt gaatttgtcc
     3901 aagttcctgg ctacccatat ccagcctatg tttactatac aagcccgcat atgatgactg
     3961 cgtggtaccc agtaggacga gatgtacatg gacgaataat cgttgtgcct gttgtaatca
     4021 cctggtctca aaatacgaac cgccagcctc cggtgcccca gtatcctcct ccgcagccac
     4081 ctccaccacc accaccacct ccaccgccac caccacctcc accagcatca tcctgcagtg
     4141 ctgcgtatcc gtatgatgtt ccggattatg catgagaatt caccccacca gtgcaggctg
     4201 cctatcagaa agtggtggct ggtgtggcta atgccctggc ccacaagtat cactaagctc
     4261 gctttcttgc tgtccaattt ctattaaagg ttcctttgtt ccctaagtcc aactactaaa
     4321 ctgggggata ttatgaaggg ccttgagcat ctggattctg cctaataaaa aacatttatt
     4381 ttcattgcaa tgatgtattt aaattatttc tgaatatttt actaaaaagg gaatgtggga
     4441 ggtcagtgca tttaaaacat aaagaaatga agagctagtt caaaccttgg gaaaatacac
     4501 tatatcttaa actccatgaa agaaggtgag gctgcaaaca gctaatgcac attggcaaca
     4561 gcccctgatg cctatgcctt attcatccct cagaaaagga ttcaagtaga ggcttgattt
     4621 ggaggttaaa gttttgctat gctgtatttt acattactta ttgttttagc tgtcctcatg
     4681 aatgtctttt cactacccat ttgcttatcc tgcatctctc agccttgact ccactcagtt
     4741 ctcttgctta gagataccac ctttcccctg aagtgttcct tccatgtttt acggcgagat
     4801 ggtttctcct cgcctggcca ctcagcctta gttgtctctg ttgtcttata gaggtctact
     4861 tgaagaagga aaaacagggg gcatggtttg actgtcctgt gagcccttct tccctgcctc
     4921 ccccactcac agtgacccgg aatccctcga catggcagtc tagcactagt gcggccgcag
     4981 atctgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5041 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5101 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5161 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5221 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5281 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5341 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5401 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5461 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5521 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5581 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5641 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5701 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5761 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5821 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5881 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5941 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6001 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6061 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6121 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6181 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6241 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6301 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6361 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6421 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6481 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6541 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6601 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6661 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6721 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6781 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6841 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6901 agtgccacct gacgtggatc ccctgagggg gcccccatgg gctagaggat ccggcctcgg
     6961 cctctgcata aataaaaaaa attagtcagc catgagcttg gcccattgca tacgttgtat
     7021 ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc atgtt
//
