LOCUS       sequence-342442-        6020 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses mouse syncytin A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Segel M, Lash B, Song J, Ladha A, Liu CC, Jin X, Mekhedov SL, Macrae
            RK, Koonin EV, Zhang F
  TITLE     Mammalian retrovirus-like protein PEG10 packages its own mRNA and 
            can be pseudotyped for mRNA delivery.
  JOURNAL   Science. 2021 Aug 20;373(6557):882-889. doi: 
            10.1126/science.abg6155.
  PUBMED    34413232
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-08-20- 20"; volume: "373"; 
            issue: "6557"; pages: "882-889"
COMMENT     Sequence Label: pCMV-SynA
FEATURES             Location/Qualifiers
     source          1..6020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          338..813
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      881..890
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2822..3216
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(3555..3572)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3726..4314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3806..3825)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4485..5276,5277..5345))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5108..5127
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5346..5450)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5520..5539)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        5641..6020
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
      301 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
      361 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
      421 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
      481 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
      541 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
      601 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
      661 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
      721 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
      781 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
      841 ctggcccatc actttggcaa agcacgtgag atctgaattc gccaccatgg ttcgtccttg
      901 ggttttctgt ctcctcctgt tcccttgttc ctctgcctac tcggacagct ggatgcccct
      961 ggtaaacctc actcaacacc tcctccagga ggctaactct tccttctctt ccaactgctg
     1021 ggtctgctta tccatccaaa cccagcgctc tctagccatg ccagccccac taaggacttg
     1081 gacagagaca cccatgaaac ttcgaatcat gtactcagcc cggaccctct ccggcccata
     1141 ccctatcacc gaccttgaga ggcgcctcca gaatttccaa ccattgactc cccactcctc
     1201 ttttgtcaac cctgaccagc gggccattgc tttccttcag atcaccagcg tgacaggcat
     1261 acttcccata ctttctcgga tcacctcggt gagatacccc gatgaccacg tctatgaatc
     1321 tgcccagcgc cccatatggg gctcactctc cacccagacg atcctcacct cccaggcccc
     1381 tctctgcata tcccgcttct tcaagaattc aaaccatgcc accttcgtgg gcaaactccc
     1441 tgcctctctt tgcaatcaca cctttcagct ctccccctct gccaaccacc aatccataga
     1501 tctgtcctcc agctatgcat tcgccccatt aatggccatg ccagggtcta aatggagaaa
     1561 ccccttacgc ttttcaggac ccccttccct gaactcaggg atgcctcact actcctgccc
     1621 gatagatgac atccactgcc acacctaccc caccaccccc tggaggtcct gtccttcctt
     1681 cccagctagc acctgctata atctcaccct attcgagccg gacaattcga gccaccctat
     1741 taccctgtct gtggacacca catacttcaa gattaaactc cagggacaca aagaccccta
     1801 tccactcttt cagtaccagc ccctcatggg ggcagccctc tctggacaat attcaatctg
     1861 ggaatatgaa cccactgtta agaaaaacgg cggtatcact ccaaatatct tctcccacct
     1921 tgtctcctta acatactcct tctgcctcaa ctcctctggt gttttcttcc tctgtggaaa
     1981 ctcaacttat gtctgtctcc cggccaattg gtccggcgtc tgtacccttg tcttccaata
     2041 cccggatatt gaactccttc ccaacaacca aaccatatct gtcccgcttt ttgctacagt
     2101 tccctcctct gtccccgctt ctcgccggaa gcgagccctt cctctccttc ctctcctcgc
     2161 gggcctgggc attgcttctg ccctggggtt aggcatcgcg ggtatcacca cctcaactgt
     2221 gtatttccaa cagctttcca aggctctctc ggacagccta gatgaaatag ccacctccat
     2281 catcagcctc caagaccaaa tagactcgct ggcgggtgtc gttctccaaa accgcagagc
     2341 tctggacctc attgtggctg agaggggggg cacctgcctc ttcctccagg aagagtgctg
     2401 cttctacata aaccagtccg gggtagtccg gcacgcggca aggaaacttc gagaaagggc
     2461 ctcggaactc ggcacaagct cgagttcttg gatccagtgg ctggggctag gaccctggct
     2521 gccctcttgg ttgacttccc tcatggctcc cattctcttt atcctggtac tgctggtttt
     2581 caggccttgt cttcttaact gcctgactca ttctgtatcg cggcgaatga gttctttcat
     2641 tcacaccacc accgaaggac acgtggacaa gatccttctg cttcgagagt cccagtacaa
     2701 gagacttccc caagagcccc cggaggagga tgccgtctag gaattcaccc caccagtgca
     2761 ggctgcctat cagaaagtgg tggctggtgt ggctaatgcc ctggcccaca agtatcacta
     2821 agctcgcttt cttgctgtcc aatttctatt aaaggttcct ttgttcccta agtccaacta
     2881 ctaaactggg ggatattatg aagggccttg agcatctgga ttctgcctaa taaaaaacat
     2941 ttattttcat tgcaatgatg tatttaaatt atttctgaat attttactaa aaagggaatg
     3001 tgggaggtca gtgcatttaa aacataaaga aatgaagagc tagttcaaac cttgggaaaa
     3061 tacactatat cttaaactcc atgaaagaag gtgaggctgc aaacagctaa tgcacattgg
     3121 caacagcccc tgatgcctat gccttattca tccctcagaa aaggattcaa gtagaggctt
     3181 gatttggagg ttaaagtttt gctatgctgt attttacatt acttattgtt ttagctgtcc
     3241 tcatgaatgt cttttcacta cccatttgct tatcctgcat ctctcagcct tgactccact
     3301 cagttctctt gcttagagat accacctttc ccctgaagtg ttccttccat gttttacggc
     3361 gagatggttt ctcctcgcct ggccactcag ccttagttgt ctctgttgtc ttatagaggt
     3421 ctacttgaag aaggaaaaac agggggcatg gtttgactgt cctgtgagcc cttcttccct
     3481 gcctccccca ctcacagtga cccggaatcc ctcgacatgg cagtctagca ctagtgcggc
     3541 cgcagatctg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3601 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3661 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3721 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3781 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3841 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3901 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3961 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4021 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4081 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4141 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4201 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4261 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4321 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4381 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4441 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4501 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4561 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4621 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4681 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4741 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4801 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4861 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4921 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4981 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5041 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5101 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5161 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5221 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5281 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5341 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5401 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5461 cgaaaagtgc cacctgacgt ggatcccctg agggggcccc catgggctag aggatccggc
     5521 ctcggcctct gcataaataa aaaaaattag tcagccatga gcttggccca ttgcatacgt
     5581 tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt
     5641 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     5701 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     5761 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     5821 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     5881 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     5941 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     6001 tagtcatcgc tattaccatg
//
