LOCUS       sequence-342617-         642 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Encodes codon optimized putative thermophilic PET hydrolase enzyme 
            for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Erickson E, Gado JE, Avilan L, Bratti F, Brizendine RK, Cox PA, Gill
            R, Graham R, Kim DJ, Konig G, Michener WE, Poudel S, Ramirez KJ, 
            Shakespeare TJ, Zahn M, Boyd ES, Payne CM, DuBois JL, Pickford AR, 
            Beckham GT, McGeehan JE
  TITLE     Sourcing thermotolerant poly(ethylene terephthalate) hydrolase 
            scaffolds from natural diversity.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7850. doi: 10.1038/s41467-022-35237-x.
  PUBMED    36543766
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7850. doi"; pages: " 
            10.1038/s41467-022-35237-x"
COMMENT     Sequence Label: pEE024
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 catatgcaac cgtacccggt cggtactcgt accattactt accaggatcc ggtacgtaac
       61 aaccgcaaca tccagacgta cctgtactat ccggcgaccg cagccggtgc taaccagcct
      121 gttgctggtg gtcagtttcc ggtcgtagtg gtggggcacg gtttcactat gaattacgcg
      181 ccgtatgcgt tttggggtaa cgcgctggct gagtctggtt atatcgtagc tatcccgaac
      241 acggaaaccg gcttttctcc gtcccatagc gccttcgctg ctgatatggc tttcctggtg
      301 gcgaaactgt acaccgaaaa caccaactcc tcctcccctt tttatcagca tgttcagtac
      361 aattcttgca ttattggtca ctctatgggt ggtggatgca cttacctggc ggcccaaaac
      421 aacgcagacg tgagcgctac ggttaccttc gcagccgcag aaaccaaccc gtctgctacc
      481 gcggctgcag caaacgttaa ctgtccgtct ctggttttct ctggttccgc cgactgcatc
      541 accccgccgg ctcagcacca ggtaccgatg tataacgctc tgccggactg taaagcgtac
      601 ggcggttctt cccgcgttga cctgcaagca tgcaaactcg ag
//
