LOCUS       sequence-343134-        8486 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses codon-optimized catalytically inactive HPDL with a 
            3xFlag-V5 tag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Banh RS, Kim ES, Spillier Q, Biancur DE, Yamamoto K, Sohn ASW, Shi 
            G, Jones DR, Kimmelman AC, Pacold ME
  TITLE     The polar oxy-metabolome reveals the 4-hydroxymandelate CoQ10 
            synthesis pathway.
  JOURNAL   Nature. 2021 Sep 1. pii: 10.1038/s41586-021-03865-w. doi: 
            10.1038/s41586-021-03865-w.
  PUBMED    34471290
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Sep 1. pii: 10.1038/s41586-021-03865-w. doi: 
            10.1038/s41586-021-03865-w."
COMMENT     Sequence Label: pLenti6.2-coHPDL H258A 3xFlag-V5
FEATURES             Location/Qualifiers
     source          1..8486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     233..252
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        314..361
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             380..778
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             865..1098
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1170..1291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1207..1226)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1261..1280
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1331..1466
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(1487..1505)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1515..1532)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1515..1531)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1524..1546)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1673..2128
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1760..1779)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1970..1991
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2154..2258
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2259..2327,2328..3119)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2477..2496)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3290..3878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3779..3798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4032..4049
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4166..4187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4202..4207,4208..4225,4226..4232)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4240..4256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4245..4267
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4264..4280
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4264..4280
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4299..4319
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4301..4319
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4347..4573
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4574..4754
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4801..4926
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5419..5652
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5837..5881
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        6226..6528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6529..6732
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6683..6703
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        6777..6795
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6883..6907
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6911..6920
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8027..8051)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8053..8118
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             8137..8178
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8213..8254
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        join(8287..8486,1..300)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8322..8345)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
       61 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
      121 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
      181 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
      241 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
      301 actctagaca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
      361 caaggtgagg aactaaacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga
      421 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
      481 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
      541 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
      601 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
      661 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
      721 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagc
      781 acaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
      841 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
      901 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      961 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     1021 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     1081 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     1141 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     1201 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     1261 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     1321 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     1381 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     1441 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     1501 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     1561 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     1621 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     1681 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     1741 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     1801 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     1861 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     1921 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     1981 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     2041 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     2101 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     2161 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2221 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     2281 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     2341 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2401 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2461 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2521 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2581 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2641 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2701 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2761 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2821 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2881 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2941 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     3001 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3061 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3121 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3181 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3241 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3301 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3361 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3421 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3481 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3541 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3601 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3661 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3721 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3781 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3841 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3901 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3961 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4021 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     4081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     4141 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     4201 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     4261 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     4321 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     4381 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     4441 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     4501 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     4561 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4621 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4681 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4741 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     4801 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     4861 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     4921 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     4981 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     5041 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     5101 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     5161 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     5221 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     5281 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     5341 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     5401 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     5461 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     5521 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     5581 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     5641 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     5701 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     5761 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     5821 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     5881 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     5941 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     6001 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     6061 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     6121 gaacggatct cgacggtatc gatgtcgacg ttaacgctag cgactgggtc tgcgatgtcg
     6181 acgttaacgc tagcgactgg gtctgcgata agcttgggag ttccgcgtta cataacttac
     6241 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     6301 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     6361 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     6421 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     6481 ctttcctact tggcagtaca tctagtatta gtcatcgcta ttaccatggt gatgcggttt
     6541 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     6601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     6661 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     6721 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
     6781 acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgagc
     6841 tcggatccac tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa
     6901 gcaggcttca ccatggcggc gccagcgctc cgtctgtgtc acattgcgtt ccacgttccg
     6961 gccggtcaac cactggcgcg taatctgcag cgtctgtttg gctttcagcc actggccagc
     7021 cgtgaagttg atggttggcg ccagctggcg ctccgtagtg gcgatgcggt ttttctggtg
     7081 aatgagggtg ccggcagtgg cgaaccactg tatggtcttg atccacgcca tgcggtgcca
     7141 agtgcgacca atctgtgctt cgatgtggcg gatgccggtg cggccacccg cgaactggcg
     7201 gcgctgggtt gtagtgttcc ggttccgccg gttcgtgttc gcgatgcgca aggtgccgcg
     7261 acgtatgcgg ttgttagcag cccggcgggt attctgagtc tcacgctgct ggaacgtgcg
     7321 ggttatcgtg gtccatttct gccgggcttt cgtccggtta gcagtgcccc gggtccgggt
     7381 tgggttagtc gtgttgacca tctgacgctg gcgtgcaccc cgggtagtag cccaacgctg
     7441 ctccgttggt ttcacgactg tctgggcttc tgccatctgc cgctgagtcc gggcgaagat
     7501 ccggaactgg gtctggaaat gacggccggc tttggtctcg gtggtctgcg tctgacggcc
     7561 ctccaagccc aaccgggcag cattgttcca accctcgttc tggcggaaag tctgccgggt
     7621 gccacgaccc gtcaagatca agtggaacag tttctggcgc gccacaaagg tccgggtctc
     7681 caagctgtgg gcctctacac cccgaatatt gttgaagcca ccgaaggtgt tgcgaccgcc
     7741 ggtggccaat ttctggcccc accgggcgcc tactaccaac aaccgggcaa agagcgccaa
     7801 attcgcgcgg ccggtcacga accacatctg ctggcccgcc aaggtattct gctcgatggc
     7861 gacaagggca agtttctgct ccaagttttc accaagagtc tgttcaccga agacaccttc
     7921 tttctggagc tgatccagcg tcaaggcgcc acgggttttg gccaaggcaa catccgtgcg
     7981 ctgtggcaga gtgttcaaga acagagcgcc cgtagccaag aagcccaccc agctttcttg
     8041 tacaaagtgg ttgattacaa agaccatgac ggtgattata aagatcatga catcgactac
     8101 aaggatgacg atgacaaggg ctcgggctcg acctcgggta agcctatccc taaccctctc
     8161 ctcggtctcg attctacatg ataactcgag tctagagggc ccgcggttcg aaggtaagcc
     8221 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gacgatggta
     8281 cctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     8341 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     8401 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     8461 tagtcaggaa gttccccccc gccccg
//
